Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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A Study on Anti-inflammatory and Antioxidant Effect of Fraxinus rhynchophylla Using Carbohydrate-Hydrolyzing Enzymes

당분해효소를 이용한 물푸레나무 효소처리물의 항염 및 항산화 효능 연구

  • Hye Won Lee (Skin & Natural Products Lab., Kolmar Korea Co., Ltd.) ;
  • You Ah Kim (Skin & Natural Products Lab., Kolmar Korea Co., Ltd.) ;
  • Byoung Jun Park (Skin & Natural Products Lab., Kolmar Korea Co., Ltd.) ;
  • Sang Keun Han (Skin & Natural Products Lab., Kolmar Korea Co., Ltd.)
  • 이혜원 (한국콜마(주) 피부천연물연구소) ;
  • 김유아 (한국콜마(주) 피부천연물연구소) ;
  • 박병준 (한국콜마(주) 피부천연물연구소) ;
  • 한상근 (한국콜마(주) 피부천연물연구소)
  • Received : 2023.08.14
  • Accepted : 2023.09.21
  • Published : 2023.09.30
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Abstract

Fraxinus rhynchophylla Hance (F. rhynchophylla) is a traditional medicinal plant that has been widely used in East Asia and has been used for chronic bronchitis, bacterial dysentery and improved eyesight. F. rhynchophylla contains various type of coumarins such as esculin, esculetin, fraxin and fraxetin. Esculetin possesses versatile activities including antioxidant, anti-inflammatory, antimicrobial, anticancer properties and improvement of atopic dermatitis. However, there is no research on the process of increasing active components in F. rhynchophylla. The objectives of the present study were to apply biotransformation technology to F. rhynchophylla for increasing the content of esculetin, and enhancing anti-inflammatory and antioxidant activities. F. rhynchophylla extract (FRE) treated with viscozyme L (FRE-VL) showed 3.1 times higher content of esculetin than FRE, and exhibited effects such as increased anti-inflammatory activity and DPPH radical scavenging activity. Based on the these results, it is concluded that biotransformed FRE-VL could be potentially applicable as a new active ingredient in the cosmetic field.

물푸레나무(Fraxinus rhynchophylla Hance, F. rhynchophylla)는 동아시아에서 널리 사용되어 온 전통적인 약용 식물로, 만성 기관지염, 세균성 이질과 시력 개선 등을 위해 사용돼 왔다. 물푸레나무에는 esculin, esculetin, fraxin 및 fraxetin과 같은 다양한 형태의 쿠마린이 존재하며, esculetin은 항산화, 항염, 항균, 항암 및 아토피성 피부 염증 완화 등의 효과를 가지고 있다. 지금까지 물푸레나무 내 유효 성분을 증가시키는 공정개발에 관한 연구는 보고되지 않았다. 따라서 본 연구의 목적은 물푸레나무 내 esculetin함량을 증가시키고, 항염 및 항산화 효능을 증대시키기 위한 물푸레나무 생물전환 공정을 개발하는 것이다. 당분해효소 viscozyme L을 이용하여 물푸레나무에 처리한 결과 물푸레나무 효소처리물(FRE-VL)은 물푸레나무 추출물(FRE)대비 esculetin 함량이 3.1배 증가하였으며, 항염 활성 증대와 DPPH 라디칼 소거 활성의 효과를 나타내었다. 본 연구를 통해 화장품 분야에서 생물전환 기술을 적용한 물푸레나무 효소처리물의 신규 화장품 기능성 소재로의 활용 가능성을 확인하였다.

Keywords

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