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Characterization of the xaiF Gene Encoding a Novel Xylanase-activity- increasing Factor, XaiF

  • Cho, Ssang-Goo;Choi, Yong-Jin
    • Journal of Microbiology and Biotechnology
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    • v.8 no.4
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    • pp.378-387
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    • 1998
  • The DNA sequence immediately following the xynA gene of Bacillus stearothermophilus 236 [about l-kb region downstream from the translational termination codon (TAA) of the xynA gene]was found to have an ability to enhance the xylanase activity of the upstream xynA gene. An 849-bp ORF was identified in the downstream region, and the ORF was confirmed to encode a novel protein of 283 amino acids designated as XaiF (xylanase-activity-increasing factor). From the nucleotide sequence of the xaiF gene, the molecular mass and pI of XaiF were deduced to be 32,006 Da and 4.46, respectively. XaiF was overproduced in the E. coli cells from the cloned xaiF gene by using the T7 expression system. The transcriptional initiation site was determined by primer extension analysis and the putative promoter and ribosome binding regions were also identified. Blast search showed that the xaiF and its protein product had no homology with any gene nor any protein reported so far. Also, in B. subtilis, the xaiF trans-activated the xylanase activity at the same rate as in E. coli. In contrast, xaiF had no activating effect on the co-expressed ${\beta}-xylosidase$ of the xylA gene derived from the same strain of B. stearothermophilus. In addition, the intracellular and extracellular fractions from the E. coli cells carrying the plasmid-borne xaiF gene did not increase the isolated xylanase activity, indicating that the protein-protein interaction between XynA and XaiF was not a causative event for the xylanase activating effect of the xaiF gene.

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SOME CONDITIONS ON THE FORM OF THIRD ELEMENT FROM DIOPHANTINE PAIRS AND ITS APPLICATION

  • Lee, June Bok;Park, Jinseo
    • Journal of the Korean Mathematical Society
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    • v.55 no.2
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    • pp.425-445
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    • 2018
  • A set {$a_1,\;a_2,{\ldots},\;a_m$} of positive integers is called a Diophantine m-tuple if $a_ia_j+1$ is a perfect square for all $1{\leq}i$ < $j{\leq}m$. In this paper, we show that the form of third element in Diophantine pairs and develop some results which are needed to prove the extendibility of the Diophantine pair {a, b} with some conditions. By using this result, we prove the extendibility of Diophantine pairs {$F_{k-2}F_{k+1},\;F_{k-1}F_{k+2}$} and {$F_{k-2}F_{k-1},\;F_{k+1}F_{k+2}$}, where $F_n$ is the n-th Fibonacci number.

A Study on the Limitations of Trade Terms in the Situtations of Kobe Earthquake -with a Special Reference to Marine Insurance- (고배대지진에 기인한 정형거래조건의 문제점)

  • 강진욱
    • The Journal of Information Technology
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    • v.1 no.2
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    • pp.15-24
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    • 1998
  • C.I.F. and F.O.B. contracts are the chief terms used in international trade contracts. But, in recently, the multimodal transport which is based on the containerization and the improvement of air transport has been grown gradually, Regardless of theese change in international trade environment, most of the contract of sale is made by C.I.F. and F.O.B. contracts which are based on the traditional port to port transport. In other words, there are some limitation in terms of legal base in which traditional C.I.F. and F.O.B. contract is applied to the changed environment. Especially, problems arised in marine insurance which export by F.O.B. trade terms. Therefore, when the parties of the contracts of sale make an sale contracts by using the container ship and Multimodal Transport, they should use the F.C.A. and C.I.P. contracts Instead of F.O.B. and C.I.F. contracts for the transport of goods. And parties of the contracts of sale need to gain a better understanding of the characteristic of F.C.A. and C.I.P. terms and the problem of the F.C.A. and C.I.P. contracts used in the performance on international multimodal transport.

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Behavior of $CaF_2$ at the Initial Adsorption Stage on Si(114)

  • Dugerjav, Otgonbayar;Duvjir, Ganbat;Li, Huiting;Kim, Hui-Dong;Seo, Jae-Myeong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.242-242
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    • 2012
  • From the combined studies of STM and synchrotron photoemission, it has been found that a $CaF_2$ molecule is dissociated to Ca and F atoms on the $Si(114)-2{\times}1$ held at $500^{\circ}C$ at the initial adsorption stage. The Ca atoms form isolated and unique shapes of silicide molecules as shown in Fig. (a), while the F atoms are desorbed from the surface. On the other hand, beyond a $CaF_2$ coverage of 0.3 monolayer, as shown in Fig. (b), in addition to these silicide molecules, a 1-D facet [composed of (113) and (115) faces] adjacent to an etch pit has been observed, and F atoms are also detected from photoemission. These results imply that F atoms act as an etchant on Si(114) and CaF is adsorbed selectively on the (113) face of this facet. From the present studies, it has been concluded that, an insulating $CaF_2$ layer like that on Si(111) cannot be formed on Si(114), but a CaF-decorated nanofacet with a high aspect-ratio can be grown.

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High Yielding [18F]Fluorination Method by Fine Control of the Base

  • Lee, Sang-Ju;Oh, Seung-Jun;Chi, Dae-Yoon;Moon, Dae-Hyuk;Ryu, Jin-Sook
    • Bulletin of the Korean Chemical Society
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    • v.33 no.7
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    • pp.2177-2180
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    • 2012
  • New [$^{18}F$]F-fluorination methods using a minimized amount of precursor has been developed by controlling the base concentration. In the first method, pre-conditioning of the anion exchange cartridge with $K_2CO_3$ solution or water was carried out. The trapped [$^{18}F$]fluoride on the cartridge was then eluted by KOMs or KOTf solution. [$^{18}F$]F-Fluorination could be performed without additional base. In the second method, the QMA cartridge was preconditioned with KOMs solutions. Trapped [$^{18}F$]fluoride on the QMA was then eluted with KOMs and additional base, such as KOH, $K_2CO_3$, and $KHCO_3$, was added into the reaction vessel. Method 1 showed a [$^{18}F$]F-incorporation yield of 20.9% for [$^{18}F$]FLT synthesis with 5 mg of precursor. Unlike method 1, a [$^{18}F$]F-incorporation yield of 91.4% was achieved from the same amount of precursor in method 2.

RADIUS CONSTANTS FOR FUNCTIONS ASSOCIATED WITH A LIMACON DOMAIN

  • Cho, Nak Eun;Swaminathan, Anbhu;Wani, Lateef Ahmad
    • Journal of the Korean Mathematical Society
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    • v.59 no.2
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    • pp.353-365
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    • 2022
  • Let 𝓐 be the collection of analytic functions f defined in 𝔻 := {ξ ∈ ℂ : |ξ| < 1} such that f(0) = f'(0) - 1 = 0. Using the concept of subordination (≺), we define $$S^*_{\ell}\;:=\;\{f{\in}A:\;\frac{{\xi}f^{\prime}({\xi})}{f({\xi})}{\prec}{\Phi}_{\ell}(\xi)=1+{\sqrt{2}{\xi}}+{\frac{{\xi}^2}{2}},\;{\xi}{\in}{\mathbb{D}}\}$$, where the function 𝚽(ξ) maps 𝔻 univalently onto the region Ω bounded by the limacon curve (9u2 + 9v2 - 18u + 5)2 - 16(9u2 + 9v2 - 6u + 1) = 0. For 0 < r < 1, let 𝔻r := {ξ ∈ ℂ : |ξ| < r} and 𝒢 be some geometrically defined subfamily of 𝓐. In this paper, we find the largest number 𝜌 ∈ (0, 1) and some function f0 ∈ 𝒢 such that for each f ∈ 𝒢 𝓛f (𝔻r) ⊂ Ω for every 0 < r ≤ 𝜌, and $${\mathcal{L} _{f_0}}({\partial}{\mathbb{D}_{\rho})\;{\cap}\;{\partial}{\Omega}_{\ell}\;{\not=}\;{\emptyset}$$, where the function 𝓛f : 𝔻 → ℂ is given by $${\mathcal{L}}_f({\xi})\;:=\;{\frac{{\xi}f^{\prime}(\xi)}{f(\xi)}},\;f{\in}{\mathcal{A}}$$. Moreover, certain graphical illustrations are provided in support of the results discussed in this paper.

VARIOUS SHADOWING PROPERTIES FOR INVERSE LIMIT SYSTEMS

  • Lee, Manseob
    • Journal of the Chungcheong Mathematical Society
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    • v.29 no.4
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    • pp.657-661
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    • 2016
  • Let $f:X{\rightarrow}X$ be a continuous surjection of a compact metric space and let ($X_f,{\tilde{f}}$) be the inverse limit of a continuous surjection f on X. We show that for a continuous surjective map f, if f has the asymptotic average, the average shadowing, the ergodic shadowing property then ${\tilde{f}}$ is topologically transitive.

VALUE DISTRIBUTION OF SOME q-DIFFERENCE POLYNOMIALS

  • Xu, Na;Zhong, Chun-Ping
    • Bulletin of the Korean Mathematical Society
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    • v.53 no.1
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    • pp.29-38
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    • 2016
  • For a transcendental entire function f(z) with zero order, the purpose of this article is to study the value distributions of q-difference polynomial $f(qz)-a(f(z))^n$ and $f(q_1z)f(q_2z){\cdots}f(q_mz)-a(f(z))^n$. The property of entire solution of a certain q-difference equation is also considered.

Optimal Rates of Convergence for Tensor Spline Regression Estimators

  • Koo, Ja-Yong
    • Journal of the Korean Statistical Society
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    • v.19 no.2
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    • pp.105-112
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    • 1990
  • Let (X, Y) be a pair random variables and let f denote the regression function of the response Y on the measurement variable X. Let K(f) denote a derivative of f. The least squares method is used to obtain a tensor spline estimator $\hat{f}$ of f based on a random sample of size n from the distribution of (X, Y). Under some mild conditions, it is shown that $K(\hat{f})$ achieves the optimal rate of convergence for the estimation of K(f) in $L_2$ and $L_{\infty}$ norms.

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