• Korean Journal of Veterinary Service
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• v.45 no.1
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• pp.19-30
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• 2022

In this study, a simple loop-mediated isothermal amplification (LAMP) combined with visual detection method (vLAMP) assay was developed for the rapid and specific detection of African swine fever virus (ASFV), overcoming the shortcomings of previously described LAMP assays that require additional detection steps or pose a cross-contamination risk. The assay results can be directly detected by the naked eye using hydroxynaphthol blue after incubation for 40 min at 62℃. The assay specifically amplified ASFV DNA and no other viral nucleic acids. The limit of detection of the assay was <50 DNA copies/reaction, which was ten times more sensitive than conventional polymerase chain reaction (cPCR) and comparable to real-time PCR (qPCR). For clinical evaluation, the ASFV detection rate of vLAMP was higher than cPCR and comparable to OIE-recommended qPCR, showing 100% concordance, with a κ value (95% confidence interval) of 1 (1.00~1.00). Considering the advantages of high sensitivity and specificity, no possibility for cross-contamination, and being able to be used as low-cost equipment, the developed vLAMP assay will be a valuable tool for detecting ASFV from clinical samples, even in resource-limited laboratories.

• Journal of Microbiology and Biotechnology
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• v.33 no.8
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• pp.1091-1100
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• 2023

Human papillomavirus (HPV) types 16 and 18 are the major causes of cervical lesions and are associated with 71% of cervical cancer cases globally. However, public health infrastructures to support cervical cancer screening may be unavailable to women in low-resource areas. Therefore, sensitive, convenient, and cost-efficient diagnostic methods are required for the detection of HPV16/18. Here, we designed two novel methods, real-time ERA and ERA-LFD, based on enzymatic recombinase amplification (ERA) for quick point-of-care identification of the HPV E6/E7 genes. The entire detection process could be completed within 25 min at a constant low temperature (35-43℃), and the results of the combined methods could be present as the amplification curves or the bands presented on dipsticks and directly interpreted with the naked eye. The ERA assays evaluated using standard plasmids carrying the E6/E7 genes and clinical samples exhibited excellent specificity, as no cross-reaction with other common HPV types was observed. The detection limits of our ERA assays were 10⁰ and 10¹ copies/µl for HPV16 and 18 respectively, which were comparable to those of the real-time PCR assay. Assessment of the clinical performance of the ERA assays using 114 cervical tissue samples demonstrated that they are highly consistent with real-time PCR, the gold standard for HPV detection. This study demonstrated that ERA-based assays possess excellent sensitivity, specificity, and repeatability for HPV16 and HPV18 detection with great potential to become robust diagnostic tools in local hospitals and field studies.

• Journal of Digital Convergence
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• v.13 no.6
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• pp.151-156
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• 2015

In this paper, we have proposed personal multimodal biometric authentication system based on face detection, recognition and speaker verification for smart-phone environment. Proposed system detect the face with Modified Census Transform algorithm then find the eye position in the face by using gabor filter and k-means algorithm. Perform preprocessing on the detected face and eye position, then we recognize with Linear Discriminant Analysis algorithm. Afterward in speaker verification process, we extract the feature from the end point of the speech data and Mel Frequency Cepstral Coefficient. We verified the speaker through Dynamic Time Warping algorithm because the speech feature changes in real-time. The proposed multimodal biometric system is to fuse the face and speech feature (to optimize the internal operation by integer representation) for smart-phone based real-time face detection, recognition and speaker verification. As mentioned the multimodal biometric system could form the reliable system by estimating the reasonable performance.

• Journal of Korea Multimedia Society
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• v.16 no.12
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• pp.1385-1392
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• 2013

There is a variety of researches about recognition system using biometric data these days. In this study, we propose a new algorithm, uses simultaneous equation that made of the edge of objects, to segment an iris region without threshold values from an anterior eye image. The algorithm attempts to find a center area through calculated outskirts information of an iris, and decides the area where the most points are accumulated. To verify the proposed algorithm, we conducted comparative experiments to Hough transform and Daugman's method, based on 50 images anterior eye images. It was found that proposed algorithm is 5 and 75 times faster than on each algorithm, and showed high accuracy of detecting a center point (95.36%) more than Hough transform (92.43%). In foreseeable future, this study is expected to useful application in diverse department of human's life, such as, identification system using an iris, diagnosis a disease using an anterior image.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.46 no.4
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• pp.70-76
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• 2009

We proposed the device to control a computer mouse with only head movements and eye blinks so that disabilities by car or other accidents can use a computer. The mouse position were estimated from a gyro-sensor which can measure head movements, and the mouse events such as click/double click were from opto sensors which can detect the eyes flicker, respectively. The sensor was mounted on the goggle in order not to disturb the visual field. There was no difference in movement speed between ours and a general mouse, but it required 3$\sim$4 more times in the result of the experiment to evaluate spatial movements and events detection of the proposed mouse because of the low accuracy. We could eliminate cumbersome work to periodically remove the accumulated error and intuitively control the mouse using non-linear relative point method with dead zones. Optical sensors are used in the event detection circuitry designed to remove the influence of the ambient light changes, therefore it was not affected in the change of external light source.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.4C
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• pp.494-504
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• 2004

The researches about gaze detection have been much developed with many applications. Most previous researches only rely on image processing algorithm, so they take much processing time and have many constraints. In our work, we implement it with a computer vision system setting a IR-LED based single camera. To detect the gaze position, we locate facial features, which is effectively performed with IR-LED based camera and SVM(Support Vector Machine). When a user gazes at a position of monitor, we can compute the 3D positions of those features based on 3D rotation and translation estimation and affine transform. Finally, the gaze position by the facial movements is computed from the normal vector of the plane determined by those computed 3D positions of features. In addition, we use a trained neural network to detect the gaze position by eye's movement. As experimental results, we can obtain the facial and eye gaze position on a monitor and the gaze position accuracy between the computed positions and the real ones is about 4.2 cm of RMS error.

• The KIPS Transactions:PartB
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• v.12B no.4 s.100
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• pp.465-472
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• 2005

In this paper, we propose the method of manipulating the gaze direction of 3D FPS game's character by using eye gaze detection from the successive images captured by USB camera, which is attached beneath HMB. The proposed method is composed of 3 parts. At first, we detect user's pupil center by real-time image processing algorithm from the successive input images. In the second part of calibration, when the user gaze on the monitor plane, the geometric relationship between the gazing position of monitor and the detected position of pupil center is determined. In the last part, the final gaze position on the HMD monitor is tracked and the 3D view in game is controlled by the gaze position based on the calibration information. Experimental results show that our method can be used for the handicapped game player who cannot use his(or her) hand. Also, it can Increase the interest and the immersion by synchronizing the gaze direction of game player and the view direction of game character.

• Korean Journal of Agricultural Science
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• v.50 no.3
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• pp.373-382
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• 2023

The task of sorting and excluding blemished apples and others that lack commercial appeal is currently performed manually by human eye sorting, which not only causes musculoskeletal disorders in workers but also requires a significant amount of time and labor. In this study, an automated apple-sorting machine was developed to prevent musculoskeletal disorders in apple production workers and to streamline the process of sorting blemished and non-marketable apples from the better quality fruit. The apple-sorting machine is composed of an arm-rest, a main body, and a height-adjustable part, and uses object detection through a machine learning technology called 'You Only Look Once (YOLO)' to sort the apples. The machine was initially trained using apple image data, RoboFlow, and Google Colab, and the resulting images were analyzed using Jetson Nano. An algorithm was developed to link the Jetson Nano outputs and the conveyor belt to classify the analyzed apple images. This apple-sorting machine can immediately sort and exclude apples with surface defects, thereby reducing the time needed to sort the fruit and, accordingly, achieving cuts in labor costs. Furthermore, the apple-sorting machine can produce uniform quality sorting with a high level of accuracy compared with the subjective judgment of manual sorting by eye. This is expected to improve the productivity of apple growing operations and increase profitability.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.12
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• pp.92-99
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• 2004

The formation degree of sustain (ITO pattern) decides quality of PDP (Plasma Display Panel). For this reason, it makes efforts in searching defects more than 30 un as 100%. Now, the existing inspection is dependent upon naked eye or microscope in off-line PDP manufacturing process. In this study developed prototype inspection system of PDP 170 glass is based on line-scan mechanism. Developed system creates information that detects and sorts kinds of defect automatically. Designed inspection technology adopts multi-vision method by slip-beam formation for the minimum of inspection time and detection algorithm is embodied in detection ability of developed system. Designed algorithm had to make good use of kernel matrix that draws up an approach to geometry. A characteristic of defects, as pin hole, substance, protrusion, are extracted from blob analysis method. Defects, as open, short, spots and et al, are distinguished by line type inspection algorithm. In experiment, we could have ensured ability of inspection that can be detected with reliability of up to 95% in about 60 seconds.

• Smart Structures and Systems
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• v.25 no.4
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• pp.423-432
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• 2020

In this paper, a novel but practical approach named series/parallel multi-sensing technique was proposed to evaluate the bolt looseness in a bolt group. The smart washers (SWs), which were fabricated by embedding a Lead Zirconate Titanate (PZT) transducer into two flat metal rings, were installed to the bolts group. By series connection of SWs, the impedance signals of different bolts can be obtained through only one sweep. Therefore, once the loosening occurred, the shift of different peak frequencies can be used to locate which bolt has loosened. The proposed multi input single output (MISO) damage detection scheme is very suitable for the structural health monitoring (SHM) of joint with a large number of bolts connection. Another notable contribution of this paper is the proposal of 3-dB bandwidth root mean square deviation (3 dB-RMSD) which can quantitatively evaluate the severity of bolt looseness. Compared with the traditional naked-eye observation method, the equivalent circuit based 3-dB bandwidth can accurately define the calculation range of RMSD. An experiment with three bolted connection specimens that installed the SWs was carried out to validate our proposed approach. Experimental result shows that the proposed 3 dB-RMSD based multi-sensing technique can not only identify the loosened bolt but also monitor the severity of bolt looseness.