• Journal of Microbiology and Biotechnology
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• v.29 no.10
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• pp.1675-1681
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• 2019

Clostridium difficile toxin A is known to cause colonic epithelial cell apoptosis, which is considered the main causative event that triggers inflammatory responses in the colon, reflecting the concept that the essential role of epithelial cells in the colon is to form a physical barrier in the gut. We previously showed that toxin A-induced colonocyte apoptosis and subsequent inflammation were dependent on prostaglandin E2 ($PGE_2$) produced in response to toxin A stimulation. However, the molecular mechanism by which $PGE_2$ mediates cell apoptosis in toxin A-exposed colonocytes has remained unclear. Here, we sought to identify the signaling pathway involved in toxin A-induced, $PGE_2$-mediated colonocyte apoptosis. In non-transformed NCM460 human colonocytes, toxin A exposure strongly upregulated expression of Bak, which is known to form mitochondrial outer membrane pores, resulting in apoptosis. RT-PCR analyses revealed that this increase in Bak expression was attributable to toxin A-induced transcriptional upregulation. We also found that toxin A upregulation of Bak expression was dependent on $PGE_2$ production, and further showed that this effect was recapitulated by an Prostaglandin E2(PGE2) receptor-1 receptor agonist, but not by agonists of other EP receptors. Collectively, these results suggest that toxin A-induced cell apoptosis involves $PGE_2$-upregulation of Bak through the EP1 receptor.

• Journal of the Korean Society of Clothing and Textiles
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• v.34 no.8
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• pp.1265-1276
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• 2010

The carelessness of basic design form in education can result in a lack of creativeness and critical thinking ability that will decrease adaptability in a rapidly changing fashion industry. If the fashion education just repeats the previous education of basic form of design, it will be impossible to create original value in fashion design. It is necessary to re-establish the characterized program of the basic form of design education. This study develops concrete educational contents of the basic form of design that can be applied to fashion design through the study of the fundamentals and the concepts of the basic form of design. This study is the basis for the cultivation of talented designers with creativity, forming ability, problem solving skills, and critical thinking ability. The research method is the fundamentals, specialty publications, and designs studied. As a result, an education program for the basic form of design to be used in fashion design education was developed. The development of education contents of the basic form of design has been developed as the plane form that aims the perceptual and emotional effects through the expression of the objects, through the use of dots, lines and planes that are the basics in forming practice and the expression of the abstract images. This suggests subjects composed of the various progressive forming conditions with the abstract dots and lines. In this subject and during the process of the idea, development, and fashion design, the overall unity, harmony, and the theory of gestalt (closure, proximity, and similarity) has been obtained. As a result, the content of the subjects was developed from the perspective that comprehensive decision making skills made from the process can lead to an improved sense of the fashion design.

• Korean Institute of Interior Design Journal
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• v.20 no.3
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• pp.110-118
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• 2011

Today aesthetics expression could be a 'Everyday-life Beauty', because today art & design are closely related with a contemporary life. This thesis dealt with a everyday-life and sensitive design which sublimate from everyday-life material to the concept of aesthetics, such a purpose of this thesis establishes a base of everyday-life design through everyday-life aesthetics. This purpose made to pay attention to Japan's aesthetics concept, because Japan has been maintained and developed its own everyday-life aesthetics. Even if Japan doesn't have only everyday-life aesthetics, it is useful that is studies as some kind of a prototype. So this study analyzed about a everyday-life aesthetics and the expression method through the case-study of Japan. According to Yonagi Muneyoshi and Leonard Koren, the Wabi-Sabi has three conceptual characteristics like impermanent, incomplete, and imperfect and six expression characteristics like implication, tranquility, simplicity, coarseness, purity, and association. This study process is as follows. In the first a vocabulary of interior design was derived from three conceptual characteristics and six expression characteristics, and spatial facts was abstracted from it, and then volume, form, and then shape of composition facts of interior space was extracted from it. Finally the expression characteristics of the Wabi-Sabi in interior design was derived from these processes. In conclusion, the expression characteristics of the Wabi-Sabi in interior design is the transitional space through a Shadow, the conversional space through form and material composition, and the copying space through a natural friendship.

• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.196-205
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• 2015

A Sulfolobus-E. coli shuttle vector for an efficient expression of the target gene in S. acidocaldarius strain was constructed. The plasmid-based vector pSM21 and its derivative pSM21N were generated based on the pUC18 and Sulfolobus cryptic plasmid pRN1. They carried the S. solfataricus P2 pyrEF gene for the selection marker, a multiple cloning site (MCS) with C-terminal histidine tag, and a constitutive promoter of the S. acidocaldarius gdhA gene for strong expression of the target gene, as well as the pBR322 origin and ampicillin-resistant gene for E. coli propagation. The advantage of pSM21 over other Sulfolobus shuttle vectors is that it contains a MCS and a histidine tag for the simple and easy cloning of a target gene as well as one-step purification by histidine affinity chromatography. For successful expression of the foreign genes, two genes from archaeal origins (PH0193 and Ta0298) were cloned into pSM21N and the functional expression was examined by enzyme activity assay. The recombinant PH0193 was successfully expressed under the control of the gdhA promoter and purified from the cultures by His-tag affinity chromatography. The yield was approximately 1 mg of protein per liter of cultures. The enzyme activity measurements of PH0913 and Ta0298 revealed that both proteins were expressed as an active form in S. acidocaldarius. These results indicate that the pSM21N shuttle vector can be used for the functional expression of foreign archaeal genes that form insoluble aggregates in the E. coli system.

• JSTS:Journal of Semiconductor Technology and Science
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• v.9 no.1
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• pp.8-13
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• 2009

In this paper, discontinuous interconnect lines are modeled as a cascaded line composed of many uniform interconnect lines. The system functions of respective uniform interconnect lines are determined, followed by its time domain response. Since the time domain response expression is a transcendental form, the waveform expression is reconfigured as an approximated linear expression. The proposed model has less than 2% error in the delay estimation.

• Korean Institute of Interior Design Journal
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• v.15 no.5 s.58
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• pp.60-67
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• 2006

An architecture is a result from representation of design knowledge by architect's creative thinking, and operated only in his internal organization system. Design knowledge based upon the experience that they have achieved in the past as well as the informations they have accumulated over the time. In this point of view, the Schema that formed by problems settlement and process repetitions for the best conclusion, not only play an important part in the designer's thinking system about a specific problem but also appeared by distinctive features. This research looks to achieve a new design method by finding out how architects express traditionality in the contemporary house through schema. This research analyze contemporary house built in seoul of 1990's. The result are as followings. First, Schema is a 'frame' that already exist in the architect's thinking, will make a good guide about express the traditionality. Second, We found out several distinctive characters in the form and the space. The form is divided in five types. It is an condensed form of roof image, the metamorphosis of form grammar, the change of part details, the representation of design principle and the directly expression. Lastly, In the space, it is the borrow of the connection method of inside and external space, the three parts composition of the space, the adaptation of 'Madang' and the Independent composition of the space due to 'Chae'

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.8 s.111
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• pp.788-794
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• 2006

In this paper, a Sommerfeld integral for an impedance half-plane is considered, which is one of classical problems in electromagnetic theory. First, the integral is evaluated into two series representations which are expressed in terms of exponential integral and Lommel function, respectively. Then based on the Lommel function expansion, an exact, closed-form expression of the integral is formulated, written in terms of incomplete Weber integrals. Additionally, based on the exponential integral expansion, an approximate expression of the integral is obtained. Validity of all formulations derived in this paper is demonstrated through comparisons with a numerical integration of the integral for various situations.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.9
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• pp.3016-3033
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• 2014

The downlink performance of distributed antenna systems (DAS) with antennas selection is investigated in Rayleigh fading multicell environment, and the corresponding system capacity and bit error rate (BER) analysis are presented. Based on the moment generating function, the probability density function (PDF) and cumulative distribution function (CDF) of the effective signal to interference plus noise ratio (SINR) of the system are first derived, respectively. Then, with the available CDF and PDF, the accurate closed-form expressions of average channel capacity and average BER are further derived for exact performance evaluation. To simplify the expression, a simple closed-form approximate expression of average channel capacity is obtained by means of Taylor series expansion, with the performance results close to the accurate expression. Besides, the system outage capacity is analyzed, and an accurate closed-form expression of outage capacity probability is derived. These theoretical expressions can provide good performance evaluation for DAS downlink. It can be shown by simulation that the theoretical analysis and simulation are consistent, and DAS with antenna selection outperforms that with conventional blanket transmission. Moreover, the system performance can be effectively improved as the number of receive antennas increases.

• Journal of Digital Convergence
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• v.20 no.4
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• pp.553-563
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• 2022

In the multimedia era and contemporary art, the expression of narrative is expanding in various ways based on formal theory. And the expression form is maximized according to the use of each medium. In terms of understanding artworks, an approach that combines various expressions and media using these narratives is also possible. Therefore, this study analyzed the works of the artists by selecting three artists, Sadic Guissi Alfrazi, Lindsay Sears, and Kara Walker, who fused the media using narrative structures. Through this, it was intended to present the artistic value and significance of the work and the possibility of expanding the narrative expression of contemporary art in the future. As a result of the analysis of previous artists' works, it was shown that the phase of convergence can be derived by using the medium of narrative concepts beyond the dichotomy of content and form, confirming that digital technology and media development are changing the form of art. In addition, it was confirmed that the use of narrative structure facilitates media conversion. It is expected that discussions on the new paradigm of contemporary art will progress in the future.

• Korean Institute of Interior Design Journal
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• v.15 no.5 s.58
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• pp.289-297
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• 2006

This study was begun on the premise that architecture which has the definite disposition for survival even in the urban, social structure of that uncertainty and constructs specialty is biomorphic architecture. This study was made with the following four specific purposes largely. First, through the theoretical investigation of biomorphic architecture that appears in the tendency that mordern architecture develops into the architecture of do-stereotype and new form, its meaning and definition are examined. Second, the analysis of biomorphic architecture is investigated and the generation causes are applied architecturally and generate biomorphic architecture is made. Third, by analyzing the material expression characteristics of biomorphic architecture, the meaning and possibility of architecture, which expresses the vitality of organism, as the material expression characteristics of biomorphic architecture, are investigated. The material expression characteristics was deduced through the generation background of biomorphic architecture. The material expression characteristics of biomorphic architecture is the various modeling by smart-network and the response system of sensitivity poly-logue.