• Title/Summary/Keyword: Enterobacter aerogenes

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Formate Decarboxylation: Initial Step for Hydrogen Production by Enterobacter aerogenes (Enterobacter aerogenes에 의한 수소 생산 초기 단계인 포메이트 탈카복시 반응 연구)

  • Choi, Jinyoung;Jho, Young Choong;Ahn, Ik-Sung
    • Applied Chemistry for Engineering
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    • v.20 no.4
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    • pp.449-452
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    • 2009
  • The absence of Fe, Se, and Mo in a minimal medium prevented the production of hydrogen from the anaerobic culture of Escherichia coli MC4100. Fe, Se, and Mo are known to be cofactors of formate dehydrogenase ($FDH_{II}$) of both E. coli and Enterobacter aerogenes. Hence when these trace elements are absent in the minimal medium, hydrogen production through formate dehydrogenation would be inhibited not only in E. coli but also in E. aerogenes. Hydrogen production by E. aerogenes 413 was delayed when lacking these trace elements. Therefore, it is believed that hydrogen production of E. aerogenes is initiated not by the reoxidation of nicotinamide adenine dinucleotide (NADH) but by formate decarboxylation.

Effects of Various Chemical Substrates on Heat Resistance of Isolated Enterobacter sp. (각종 화학기질이 분리된 Enterobacter sp. 의 열저항서에 미치는 효과)

  • 정영건;권오진
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.4
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    • pp.395-400
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    • 1991
  • Effects of Various Chemical Substrates on Heat Resistance of Isolated EnterobTo pursue the various properties of Enterobacter sp. which may give an outbreak of food-borne disease to man, isolated from the meats and the small intestines of wild mice, and to offer the significant data in the fields of food hygiene and public health this study was carreied, 5 strains of Enterobacter sp. were isolated from the above samples. 5 strains isolated from sample sources were 1 strain (E. aerogenes) from the beeves, 2 strains (E. aerogenes, E intermedium) from the pork and 2 strains (E. aerogenes, E. cloacae) from the small intstines of wild mice. Of total isolatd 89 strains, including non-Enterobacter sp., these numbers of 5 isolates were showed as 5.6% of dection rate. On heating at temperature of $55^{\circ}C$ for 30min., these 5 stratins were generally more resisted in the 0.1M phosphate buffer when such chemical substrates as 0.1M glycine, 5% mannitol or 0.5% sorbitol was added to it whereas they were appeared weaker resistances in 0.1M phosphate buffer when 1% sodium citrate, 1% casein, 10% sodium chloride or 0.1M systein was infused into it.cter sp.

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Intraventricular Pefloxacine Therapy for a Cerebral Ventriculitis by Enterobacter Aerogenes - Case Report - (Enterobacter Aerogenes에 의한 뇌실염에 대한 Pefloxacine의 뇌실내 주입 - 증 례 보 고 -)

  • Lee, Jeun Haeng;Lee, Kyung Hoe;Hong, Seung Kuan
    • Journal of Korean Neurosurgical Society
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    • v.29 no.1
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    • pp.126-130
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    • 2000
  • A 40-year-old female with hypertensive thalamic hemorrhage, secondary intraventricular hemorrhage, and hydrocephalus was treated with extraventricular drainage. She developed catheter-related ventriculitis caused by gram-negative rods, Enterobacter aerogenes. She was treated with systemic pefloxacine, ceftazidime, amikin and intraventricular vancomycin, gentamicin was unsuccessful. The ventriculitis was successfully controlled by intraventricular administration of the pefloxacine. Regarding their excellent activity against gram-negative rods, Enterobacter aerogenes, and probable safety when administered intraventricularly, administration of the pefloxacine, may be considered in the treatment of ventriculitis if the pathogen is resistant to other conventional antibiotics.

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The Optimization of Biohydrogen Production Medium by Dark Fermentation with Enterobacter aerogenes (Enterobacter aerogenes의 혐기발효에 의한 바이오 수소 생산 배지의 최적화)

  • Kim, Kyu-Ho;Choi, Young-Jin;Kim, Eui-Yong
    • KSBB Journal
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    • v.23 no.1
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    • pp.54-58
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    • 2008
  • Hydrogen is considered as an energy source for the future due to its environmentally friendly use in fuel cells. A promising way is the biological production of hydrogen by fermentation. In this study, the optimization of medium conditions which maximize hydrogen production from Enterobacter aerogenes KCCM 40146 were determined. As a result, the maximum attainable cumulative volume of hydrogen was 431 $m{\ell}$ under the conditions of 0.5M potassium phosphate buffer, pH 6.5 medium containing 30 g/L glucose. The best nitrogen sources were peptone and tryptone for the cell growth as well as hydrogen production. The control of cell growth rate was found to be a important experimental parameter for effective hydrogen production

Kinetics of nitrification and acrylamide biodegradation by Enterobacter aerogenes and mixed culture bacteria in sequencing batch reactor wastewater treatment systems

  • Madmanang, Romsan;Jangkorn, Siriprapha;Charoenpanich, Jittima;Sriwiriyarat, Tongchai
    • Environmental Engineering Research
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    • v.24 no.2
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    • pp.309-317
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    • 2019
  • This study evaluated the kinetics of acrylamide (AM) biodegradation by mixed culture bacteria and Enterobacter aerogenes (E. aerogenes) in sequencing batch reactor (SBR) systems with AQUASIM and linear regression. The zero-order, first-order, and Monod kinetic models were used to evaluate the kinetic parameters of both autotrophic and heterotrophic nitrifications and both AM and chemical oxygen demand (COD) removals at different AM concentrations of 100, 200, 300, and 400 mg AM/L. The results revealed that both autotrophic and heterotrophic nitrifications and both AM and COD removals followed the Monod kinetics. High AM loadings resulted in the transformation of Monod kinetics to the first-order reaction for AM and COD removals as the results of the compositions of mixed substrates and the inhibition of the free ammonia nitrogen (FAN). The kinetic parameters indicated that E. aerogenes degraded AM and COD at higher rates than mixed culture bacteria. The FAN from the AM biodegradation increased both heterotrophic and autotrophic nitrification rates at the AM concentrations of 100-300 mg AM/L. At higher AM concentrations, the FAN accumulated in the SBR system inhibited the autotrophic nitrification of mixed culture bacteria. The accumulation of intracellular polyphosphate caused the heterotrophic nitrification of E. aerogenes to follow the first-order approximation.

Effect of pH, Temperature, and added Sucrose on the Production of Vitamin $B_{12}$ and Riboflavin by Bacillus megaterium and Enterobacter aerogenes (온도, pH 및 첨가된 Sucrose가 Bacillus megaterium 과 Enterobacter aerogenes 에 의한 비타민 $B_{12}$ 와 Riboflavin 생산에 미치는 영향)

  • Chung, Hee-Jong;Marion L. Fields
    • Microbiology and Biotechnology Letters
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    • v.15 no.2
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    • pp.112-115
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    • 1987
  • Optimal pH temperature and sucrose content for the production of vitamin B$_{12}$ and riboflavin by Bacillus megaterium and Enterobacter aerogenes was studied by microbiological analysis. Optimal pH for the production of B$_{12}$ was 6.0 by B. megaterium while the pH for E. aerogenes was 5.0. However, upon the addition of sucrose the optimal pH for B. megaterium shifted to 7.5 but E. aerogenes remained at pH 5.0. In the absence of sucrose, pH did not influence the yields of riboflavin produced by either bacterium. Addition of sucrose stimulated synthesis of riboflavin by both bacteria. Temperature had little effect on the production of vitamins by either bacterium.

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Bioethanol Production Based on Crude Glycerol Using Enterobacter aerogenes (Enterobacter aerogenes를 이용한 crude glycerol 기반의 바이오에탄올 생산)

  • Jung, Hong-Sub;Seong, Pil-Je;Go, A-Ra;Lee, Sang-Jun;Kim, Seung-Wook;Han, Sung-Ok;Cho, Jae-Hoon;Cho, Dae-Haeng;Kim, Yong-Hwan;Park, Chul-Hwan
    • KSBB Journal
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    • v.26 no.3
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    • pp.223-228
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    • 2011
  • The effects of pH, glycerol concentration and salt on cell growth and ethanol production using Enterobacter aerogenes KCTC 2190 were evaluated in the anaerobic culture condition. In condition of initial pH 5, cell growth and ethanol production were highest. An initial concentration of 10 g/L of pure glycerol gave the highest cell growth and ethanol production. However, in case of over 15 g/L of pure glycerol, they decreased. The cell growth and ethanol production decreased with the increase of salt concentration. When 10 g/L of crude glycerol was used as the carbon source, the cell growth and ethanol production were $1.32\;OD_{600}$ and 3.95 g/L, respectively, which were about 94.4% and 88.5% compared to those of pure glycerol. These result indicates that the crude glycerol produced in the biodiesel manufacturing process maybe useful as a potential carbon source for ethanol production form Enterobacter aerogenes KCTC 2190.

Construction of the Phosphate-Limitation Inducible Expression Vector Containing the phoA Promoter of Enterobacter aerogenes (Enterobacter aerogenes 의 phoA 유전자 Promoter를 이용한 인 제한환경에서 발현하는 벡터 구축)

  • 장화형;고병훈;박신영;이성호;김성진;임유정;한갑진;김영호;이영근
    • Korean Journal of Microbiology
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    • v.38 no.4
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    • pp.318-321
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    • 2002
  • To induce recombinant protein under phosphate restricted conditions such as soil, we have constructed the expression vector (pEAAP) with phoA gene promoter of Enterobacter aerogenes. To construct the pEAAP, deletion of the T7 promoter and lac operator from pET-22b(+) by BglII-XhoI digestion and addition of the phoA gene promoter (containing the pho box) were performed. To test pEAAP as an expression vector controled by phosphate limitation, pEAPHY1 was constructed with the phytate gene (Bsa-phy1) of Bacillus subtillis var. amyloliquefaciens (KCTC 8913P). Under the phosphate-limitation condition, CK-PHY1 ( Escherichia coli JM109 was transformed with pEAPHY1) expressed the 41 kD Bsa-Phy1 . Also CK-PHY1 formed the clear zone in solid medium containing phytate as a sole phosphate source.

Screening for Antimicrobial Activity from Korean Plants (국내산 식물의 항균활성 검색)

  • 최원균;김용성;조규성;성창근
    • The Korean Journal of Food And Nutrition
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    • v.15 no.4
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    • pp.300-306
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    • 2002
  • 141 methanol extracts from 125 plant species which populate in Korea were screened for antimicrobial activity against various food-borne pathogens and food spoilage microorganisms. Those plants were selected from 3 different plant groups: traditional herbs, edible plants and flowers. The methanol extracts were tested by using the disk diffusion assay against five bacteria: Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter aerogenes, Escherichia coli. From the evaluation of the inhibition zone diameter of microbial growth, the most significant antimicrobial activity against Bacillus subtilis, Staphylococcus auresus, Pseudomonas aeruginosa, Enterobacter aerogenes, Escherichia coli was observed from the extract of Schizandra chinensis (Turcz.) Baill., Rheum officinale Baill., Schizandra chinensis (Turcz.) Baill., Koelreuteria paniculata Lax and Crataegus pinnatifida Bunge, respectively. The extract from many plants - Koelreuteria paniculata Lax, Chaenomeles sinensis Koehne, Scutellaria bacicalensis Georgi, Castanea crenata Sieb. et Zucc., Rosa centifolia L., Allium fistulosum L. var. giganteum Makino, Crataegus pinnatifida Bunge, Schizandra chinensis (Turcz.) Baill., Lonicera japonica - showed antimicrobial activity all four tested bacteria.

Antimicrobial Activity of Water Soluble Propolis (수용성 프로폴리스의 항균성)

  • Park, Heon-Kuk;Kim, Sang-Bum;Shim, Chang-Hwan
    • The Korean Journal of Food And Nutrition
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    • v.21 no.1
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    • pp.15-21
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    • 2008
  • In this study, the minimum inhibition concentration(MIC), growth inhibition activity, and colony forming inhibitory activity of water soluble propolis against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae and Salmonella enteritidis were tested. The MICs of the water soluble propolis against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella enteritidis were 312.5 ppm, below 156.3 ppm, 625 ppm, 10,000 ppm, above 10,000 ppm, 10,000 ppm, above 10,000 ppm, above 10,000 ppm, 10,000 ppm, and above 10,000 ppm, respectively. The growth inhibition concentrations against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, and Klebsiella pneumoniae were 156.3 ppm, below 156.3 ppm, 625 ppm, 5,000 ppm, 10,000 ppm, 10,000 ppm, 10,000 ppm, 10,000 ppm, and 5,000 ppm, respectively. However, 10,000 ppm did not inhibit the growth of Salmonella enteritidis. Finally, the colony forming inhibitory activities against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella enteritidis were 98.0%, 99.8%, 69.8%, 98.1%, 62.0%, 63.1%, 79.5%, 61.9%, 79.6%, and 0.0%, respectively.