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Construction of the Phosphate-Limitation Inducible Expression Vector Containing the phoA Promoter of Enterobacter aerogenes  

장화형 (배재대학교 바이오의약연구센터(Bio-Med RRC))
고병훈 (배재대학교 자연과학대학 생물의약학과)
박신영 (배재대학교 자연과학대학 생물의약학과)
이성호 (배재대학교 자연과학대학 생물의약학과)
김성진 (배재대학교 자연과학대학 생물의약학과)
임유정 (배재대학교 자연과학대학 생물의약학과)
한갑진 (배재대학교 자연과학대학 생물의약학과)
김영호 (배재대학교 자연과학대학 생물의약학과)
이영근 (한국원자력연구소 방사선응용부)
Publication Information
Korean Journal of Microbiology / v.38, no.4, 2002 , pp. 318-321 More about this Journal
Abstract
To induce recombinant protein under phosphate restricted conditions such as soil, we have constructed the expression vector (pEAAP) with phoA gene promoter of Enterobacter aerogenes. To construct the pEAAP, deletion of the T7 promoter and lac operator from pET-22b(+) by BglII-XhoI digestion and addition of the phoA gene promoter (containing the pho box) were performed. To test pEAAP as an expression vector controled by phosphate limitation, pEAPHY1 was constructed with the phytate gene (Bsa-phy1) of Bacillus subtillis var. amyloliquefaciens (KCTC 8913P). Under the phosphate-limitation condition, CK-PHY1 ( Escherichia coli JM109 was transformed with pEAPHY1) expressed the 41 kD Bsa-Phy1 . Also CK-PHY1 formed the clear zone in solid medium containing phytate as a sole phosphate source.
Keywords
Bacillus subtilis var. amyloliquefaciens; Enterobacter aerogenes; expression vector; phoA promoter; phosphate-limitation; phytase;
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