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Usability of DNA Sequence Data: from Taxonomy over Barcoding to Field Detection. A Case Study of Oomycete Pathogens

  • Choi, Young-Joon;Thines, Marco
    • 한국균학회소식:학술대회논문집
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    • 2015.11a
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    • pp.41-41
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    • 2015
  • Oomycetes belong to the kingdom Straminipila, a remarkably diverse group which includes brown algae and planktonic diatoms, although they have previously been classified under the kingdom Fungi. These organisms have evolved both saprophytic and pathogenic lifestyles, and more than 60% of the known species are pathogens on plants, the majority of which are classified into the order Peronosporales (includes downy mildews, Phytophthora, and Pythium). Recent phylogenetic investigations based on DNA sequences have revealed that the diversity of oomycetes has been largely underestimated. Although morphology is the most valuable criterion for their identification and diversity, morphological species identification is time-consuming and in some groups very difficult, especially for non-taxonomists. DNA barcoding is a fast and reliable tool for identification of species, enabling us to unravel the diversity and distribution of oomycetes. Accurate species determination of plant pathogens is a prerequisite for their control and quarantine, and further for assessing their potential threat to crops. The mitochondrial cox2 gene has been widely used for identification, taxonomy and phylogeny of various oomycete groups. However, recently the cox1 gene was proposed as a DNA barcode marker instead, together with ITS rDNA. To determine which out of cox1 or cox2 is best suited as universal oomycete barcode, we compared these two genes in terms of (1) PCR efficiency for 31 representative genera, as well as for historic herbarium specimens, and (2) in terms of sequence polymorphism, intra- and interspecific divergence. The primer sets for cox2 successfully amplified all oomycete genera tested, while cox1 failed to amplify three genera. In addition, cox2 exhibited higher PCR efficiency for historic herbarium specimens, providing easier access to barcoding type material. In addition, cox2 yielded higher species identification success, with higher interspecific and lower intraspecific divergences than cox1. Therefore, cox2 is suggested as a partner DNA barcode along with ITS rDNA instead of cox1. Including the two barcoding markers, ITS rDNA and cox2 mtDNA, the multi-locus phylogenetic analyses were performed to resolve two complex clades, Bremia lactucae (lettuce downy mildew) and Peronospora effuse (spinach downy mildew) at the species level and to infer evolutionary relationships within them. The approaches discriminated all currently accepted species and revealed several previously unrecognized lineages, which are specific to a host genus or species. The sequence polymorphisms were useful to develop a real-time quantitative PCR (qPCR) assay for detection of airborne inoculum of B. lactucae and P. effusa. Specificity tests revealed that the qPCR assay is specific for detection of each species. This assay is sensitive, enabling detection of very low levels of inoculum that may be present in the field. Early detection of the pathogen, coupled with knowledge of other factors that favor downy mildew outbreaks, may enable disease forecasting for judicious timing of fungicide applications.

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The Effect of Consumer's Interpersonal Anxiety on Shopping Addiction: Focusing on Multi-parallel Mediation Effect of Self-Control and Public-Consciousness and Moderating Effect between Men and Women (소비자의 대인 불안이 구매중독 성향에 미치는 영향: 자기 통제력과 타인 의식성의 다중 병렬매개효과와 남녀 간의 조절 효과를 중심으로)

  • Park, Ok-Hee;Jeong, Jo-Hee;Lee, Seok-Kee
    • Journal of Convergence for Information Technology
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    • v.10 no.2
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    • pp.70-81
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    • 2020
  • Living in the modern world, we can easily access large marts and outlets, and live convenient mobile shopping and same-day and early morning shipping. It is called 'shopping addiction' when the purpose of such shopping is not to be attributed to the original purpose of the goods, but to be purchased by an irresistible impulse. According to past studies, 5.8% of American adults and 15.5% of Korean college students were found to be purchase poisoning[3]. The study identified the relationship between interpersonal anxiety, public-consciousness and self-control among the various factors leading to purchase addiction and confirmed their impact on consumers internal psychology. The study found that although Interpersonal anxiety did not directly affect purchasing addiction, it was influenced by self-control and public-consciousness that increased the tendency to purchase addiction. The results of the group comparison between men and women showed that the self-control of men and the public-consciousness of women has increased their purchasing addiction. Such differences in psychology between men and women suggested related implications, such as the need to apply purchase addiction prevention management and treatment methods differently.

On the Location arid Development of Industrial Complexes in Chonbuk Province (전북지역 산업단지의 입지 및 발달에 관한 연구)

  • 백영기
    • Journal of the Economic Geographical Society of Korea
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    • v.6 no.2
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    • pp.307-326
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    • 2003
  • In Korea, as the state has played a vital role for industrial development, the location and development of industrial complexes have not been merely decided by economic factors but political and social factors as well. The location of industrial complexes in Chonbuk province has not been decided by industrial enterprises to be located there, but by investment strategies of the state. In the early stage of industrialization, Chonbuk could not attract a large-scale major complex because it had relatively inferior infrastructure for industry and uneasy access to overseas export markets, as compared to the capital region or the Southeast region of the country, and it has been left behind as a periphery in the national industrial system. Since 1980, the spatial policies for lessening regional disparities had much contributed to the development of industrial complexes in this province. The industrial complexes leading to the development of manufacturing activities in this region have also great influenced on structural changes of manufacturing as well as changes in the structure of the regional economy. Especially, capital goods manufacturing tends to increase in this region as the development of sectors required relative higher skill and technology mainly occurs in these complexes. And where the large-scale industrial complexes are located greatly influences the locational patterns of manufacturing in this province. The patterns of the industrial complexes in this region have concentrated in Chonju, Iksan, and Kusan, which are the largest cities in this province. Given these three cities to be very closely proximate, it seems to be easy to build network between them to develop the manufacturing activities in the near future.

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The Distribution and Treatment of Outpatients with General Anesthesia in Chonbuk National University Dental Hospital for 9 Years (최근 9년간 전북대학교 치과 병원 외래 전신마취 환자 분포 및 치료)

  • Moon, Yujin;Lee, Daewoo;Kim, Jaegon;Baik, Byeongju;Yang, Yeonmi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.42 no.2
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    • pp.158-163
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    • 2015
  • General anesthesia (GA) for dental care in handicapped patients is necessary to facilitate the provision of safe, efficient, and effective quality treatment. The aims of this study were to determine the anesthetic characteristics of handicapped patients in need of dental treatment in these day care units, and to establish for plan to provide better services. 325 patients who had outpatient general anesthesia from January 2005 to March 2014 were assessed for this study. Patients' distribution and treatment patterns were examined. The proportion of male patients (202, 62.2%) was higher than female patients (123, 37.8%) and the largest group of patients were 5 to 10 years old (85, 26.2%). The reasons for general anesthesia included mental and physical disabilities (207, 63.7%), behavior management (84, 25.8%), parent needs (14, 4.3%), and so on. Restorative treatment was the most common procedure with the average of 4.2 teeth treated per one patient and 43 (13.2%) patients underwent general anesthesia for dental treatment more than once. To expand and improve access to the dental care of the disabled, improvement of the health care system, enhancement of their training for dental care by professionals, and enlarging caregivers'understanding of the importance of oral care in the early stages are required.

Technical Analysis of an MRV System in Relation to the Implementation of a Data Collection System by the International Maritime Organization (국제해사기구 데이터수집시스템 도입에 따른 MRV 지원시스템의 기술적 분석)

  • Kang, Nam-seon;Lee, Jung-yup;Hong, Yeon-jeong;Byeon, Sang-su;Kim, Jin-yhyung
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.1
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    • pp.122-129
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    • 2017
  • This study presents the results from a technical analysis of a portal system that is compatible with MRV regulations and utilized to examine energy efficiency in international shipping, in relation to the implementation of a mandatory data collection system by the International Maritime Organization. The details of the SEEMP guidelines, including the data collection system and methods for collecting data on fuel use, were reviewed. Strategies for domestic shipping companies toward MRV have been recommended by identifying differences with the EU MRV, and the technical adequacy of the MRV system was assessed. The MRV system enhances cost and work efficiency by managing emissions data from the early stage to the final stage. It is capable of collecting and reporting emissions data while adhering to the reporting procedures of shipping companies. By granting different access privileges to users, the system supports shipping companies in their data collection and reporting, and also supports verifiers in their data verification activities. Moreover, it makes possible the submission of reports in electronic from, thereby enabling shipping companies to adopt an integrated response to international MRV regulations.

Trend of Space Development and Issue (우주개발동향과 주요 이슈)

  • Cho, Hong-Je;Shin, Yong-Do
    • The Korean Journal of Air & Space Law and Policy
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    • v.29 no.1
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    • pp.97-126
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    • 2014
  • October 4, 1957 the Soviet Sputnik 1 was launched into space the first time in the history of mankind. After launching, the realm of humankind was expanded to space. Today all countries of the world wage a fierce competition in order to utilize space for various purposes. World powers of space such as United States, Russia, China, and Japan, put reconnaissance satellites and ocean surveillance satellites into orbit, being able to easily see equipment and troops movement on earth. Each country makes efforts to occupy space assets through the militarization of space and expand national interests. Recently private companies or individuals involved in commercial space activities are becoming more prevalent. Thus, in addition to space activities for military purposes, commercial space activities become widespread. Individuals and private companies as well as nations are also involved in space activities. Outer space is not the monopoly of space powers such as the United States and Russia. The whole human race can benefit from free access to space, being the common heritage of mankind. In particular, outer space becomes an indispensable element of military activities and human life. Many countries are now entering space development, putting a lot of budget into new development programs. Republic of Korea also built the Narodo Space Center, starting its space development with budget and manpower. We have to find out ways to use space not only for military purposes but also for commercial space activities that can contribute to the national economy. In addition, through the joint efforts of the international community, we have to make efforts for preservation and peaceful use of space. Various issues relating to space activities and research should be studies in order to contribute to the progress of humanity. Those issues include the definition of outer space, space debris reduction and environmental conservation issues, non-bind measure cooperation - European International Code of Conduct, space law and national legislation related empowerment issues, arms control measures in space, and restrictions on the use of nuclear fuel. We also need to be involved in the discussion of those issues as one of responsible space countries. In addition, we try to find out regional cooperation schemes such as the ESA in the Europe actively. Currently in the Northeast Asia, cooperation bodies led by Japan and China respectively, are operated in the confrontational way. To avoid such confrontation, a new cooperative body needs to be established for cooperation on space exploration and information. The system to allow the exchange of satellite information for early warning of natural disasters needs to be built as well. In addition, efforts to enhance the effectiveness of the relevant international treaties on space, and fill in the blanks in international space laws should be made at the same time. To this end, we have to do a leading role in the establishment of standards such as non-binding measures (resolution) - Code of Conduct, being discussed in the UN and other organizations, and compliance with those standards. Courses in aerospace should be requires in law schools and educational institutes, and professional manpower need to be nurtured. In addition, the space-related technology and policy needs to be jointly studied among the private, public, and military groups, and the cross exchange among them should be encouraged.

SURGICAL APPROACH TO THE INFRAOCCLUDED TEETH BY USING SPACE REGAINING TREATMENT (저위교합 유구치에서의 공간확장술을 이용한 외과적 접근)

  • Bang, Seok-Yun;Kim, Eun-Jung;Kim, Hyun-Jung;Kim, Young-Jin;Nam, Soon-Hyeun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.3
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    • pp.531-536
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    • 2005
  • Infraclusion may be defined as teeth that stop their relative occlusal movement in the dental arches during or after the period of active eruption and then remain under the occlusal plane. Delayed exfoliation, malocclusion, increased susceptibility to dental caries and periodontal disease of both the neighboring teeth and retained molar, and dislocation of the successor are the consequencces of infraclusion of primary molars. Therefore, early diagnosis and appropriate treatments are necessary. The therapeutic approach of the infracluded teeth varied from preservation to extraction. The teeth with simple infraclusion without any signs of interference with occlusal and jaw development may be examined periodically with follow-up check and radiographically. However, if the infracluded tooth interferes with normal eruption of successor or shows any sign of delayed resorption, or the tipping of adjacent teeth or supraeruption of opposing teeth is expected, the teeth inflicted should be extracted and appropriate measures should be provided in order to maintain the normal development of occlusion and dentition. The adjacent teeth which have been collapsed over a infracluded deciduous teeth can disturb the arch length perimeter. In such cases, surgical approach might be necessary, although it would be difficult when teeth are severly leaned. However, an easier surgical access have been obtained by space regaining procedures, in young patients whose arch length has been shortened due to the infracluded teeth.

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A Development of the Unified Object-Oriented Analysis and Design Methodology for Security-Critical Web Applications Based on Object-Relational Database - Forcusing on Oracle11g - (웹 응용 시스템 개발을 위한 보안을 고려한 통합 분석·설계 방법론 개발 - Oracle11g를 중심으로 -)

  • Joo, Kyung-Soo;Woo, Jung-Woong
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.12
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    • pp.169-177
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    • 2012
  • In the development process of application systems, the most important works are analysis and design. Most of the application systems are implemented on database system. So, database design is important. Also, IT System are confronted with more and more attacks by an increase interconnections between IT systems. Therefore security-related processes belong to a very important process. Security is a complex non-functional requirement that can interaction of many parts in the system. But Security is considered in the final stages of development. Therefore, Their increases the potential for the final product to contain vulnerabilities. Accordingly, Early in development related to security analysis and design process is very important. J2EE gives a solution based on RBAC((Role Based Access Control) for security and object-relational database also has RBAC for security. But there is not a object-oriented analysis and design methodology using RBAC of J2EE and object-relational database for security. In this paper, the unified object-oriented analysis and design methodology is developed for security-critical web application systems based on J2EE and object-relational database. We used UMLsec and RBAC of object-relational database and J2EE for this methodology.

Linkage the Development Strategy of Local Government to Spatial Decision Support System (지자체 발전전략과 공간적 의사결정지원체계의 연계 방안 연구)

  • Park, Ji-Man;Hwang, Chul-Sue
    • Journal of the Korean association of regional geographers
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    • v.15 no.5
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    • pp.671-687
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    • 2009
  • This paper aims to link the development strategy of a local government(Guri-si, Kyunggi-do) to a spatial decision support system that is in the early stages of implementation and testing. Spatial Decision for a local governmental authority typically involves multiple stakeholders. To any specific regional problem, stakeholders often have various levels of knowledge and experiences about it, that yield conflicting views. In this point of view, the enhanced access to information can bring the decision process to solve the problem throughout more public participation. Recently, Urban development study was required to improve the decision making of public participation form. This study aim to investigate an analysis local government of Guri-si, can be supported as the suggestion of decision making system. Guri-si has been argued that it would improve citizen's life quality intent theme 'by historical tourism in general dimension' promote region identity, environment friendly and transportational strength, by reflecting various plan of urban development and it executed strategy. There are increasing interest in citizen participation and practical using of policy decision making to respond to uncertain environment and systematic exploration of local government. Focused on the contents of public participation decision making, it is important to investigate how to analysis urban development strategy and geographic information relationship for useful. The paper concludes with a discussion of current issues encountered during development and implementation of spatial decision making(or support) system for the local administrative authority.

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A study on analysis model for real radio spectrum data correlation in High-Mountain Area (고지대에서의 전파도달범위 분석을 위한 실측 데이터 기반 전파도달 상관관계 분석모델 연구)

  • Han, In-Sung;Sohn, Ju-Hang;Park, Moo-Sung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.5
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    • pp.697-708
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    • 2016
  • As the needs for fast wireless communication technology in various fields, including companies, individuals, etc., grows, many research projects related to the coverage of propagation are being carried out for plaining optimized communication services. On the other hand, there are some limitations in surveying and analyzing propagation in highland areas. To provide a better communication service of a new service, a range of radio environment conditions based on a wide radio bandwidth (selection of propagation model, correction of value in accordance with radio bandwidth, etc.) should be considered. In particular, radio environment conditions are becoming increasingly sophisticated. By the early detection of real-time changes in the radio spectrum, which is based on an examination and research of regional occupied band width condition, proper measures should be established. To make a proper solution of above, the basic real-time analysis of spectrum distribution status by regional groups is necessary. In addition, the establishment of prompt measures should be enable by stored or analyzed radio data. In an attempt to predict reliable propagation coverage, this thesis examines the limited propagation data with HTZ Warfare, which were collected from domestic high land sites faced with limited access. Furthermore, a comparative result value evaluation for an optimized propagation model was performed through testing simulations, and frequency-dependent and propagation model-dependent analysis.