• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.2
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• pp.307-313
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• 2001

Iron excess is known to affect long-term iron accumulation and tissue change such as fibrosis in liver. To determine the changes of expression level of genes associated with fibrosis by short-term iron exposure, we measured liver mRNA levels by reverse transcription polymerase chain reaction (RT-PCR) in rats fed dietary carbonyl iron (3%, wt/wt) for 9 weeks. The results showed that the expression of the collagen (I, III) and transforming growth factor (TGF)-$\beta$I mRNAs was enhanced in high-dose iron treated rat, compared to normal-dose iron treated rat. An electron microscopy study revealed that excess iron caused increase of collagen fibrils in liver. The cell shapes and compositions of hepatocytes and extracellular matrix(ECM) in liver were changed by the iron-treatment. Also, necrosised hepatocytes were broadly seen in ECM. Taken together, we suggest that iron overload affects changes of collagen and TGF-$\beta$I gene expression and these changes are associated with liver fibrogenesis.

• Journal of Korea Society of Industrial Information Systems
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• v.18 no.6
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• pp.95-101
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• 2013

Social media has many advantages. It can gain latest information with real time, be spread rapidly, easily be reproduced and distributed regardless of its form. These advantages can result in real time predictions using the latest information, which is possible due to the increase in social demand for more quick and accurate economic variable predictions. In this paper we adopted ARIMAX and ECM model to predict the unemployment rate and as a social information we used the Google Index provided by Google Trend. Also we used News Index as a domestic social information. The process of fitting statistical model considered in this paper can be adopted to predict various socio/economic indices as well as unemployment rate.

• Steel and Composite Structures
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• v.19 no.5
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• pp.1055-1075
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• 2015

In order to investigate the accuracy and applicability of steel equivalent constitutive model, the calculated results were compared with typical tests of steel frames under static and dynamic loading patterns firstly. Secondly, four widely used models for time history analysis of steel frames were compared to discuss the applicability and efficiency of different methods, including shell element model, multi-scale model, equivalent constitutive model (ECM) and traditional beam element model (especially bilinear model). Four-story steel frame models of above-mentioned finite element methods were established. The structural deformation, failure modes and the computational efficiency of different models were compared. Finally, the equivalent constitutive model was applied in seismic incremental dynamic analysis of a ten-floor steel frame and compared with the cyclic hardening model without considering damage and degradation. Meanwhile, the effects of damage and degradation on the seismic performance of steel frame were discussed in depth. The analysis results showed that: damages would lead to larger deformations. Therefore, when the calculated results of steel structures subjected to rare earthquake without considering damage were close to the collapse limit, the actual story drift of structure might already exceed the limit, leading to a certain security risk. ECM could simulate the damage and degradation behaviors of steel structures more accurately, and improve the calculation accuracy of traditional beam element model with acceptable computational efficiency.

• Smart Structures and Systems
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• v.7 no.3
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• pp.213-222
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• 2011

The native extracellular matrix (ECM) consists of an integrated fibrous protein network and proteoglycan-based ground (hydrogel) substance. We designed a novel electrospinning technique to engineer a three dimensional fiber-hydrogel composite that mimics the native ECM structure, is injectable, and has practical macroscale dimensions for clinically relevant tissue defects. In a model system of articular cartilage tissue engineering, the fiber-hydrogel composites enhanced the biological response of adult stem cells, with dynamic mechanical stimulation resulting in near native levels of extracellular matrix. This technology platform was expanded through structural and biochemical modification of the fibers including hydrophilic fibers containing chondroitin sulfate, a significant component of endogenous tissues, and hydrophobic fibers containing ECM microparticles.

• BMB Reports
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• v.41 no.12
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• pp.858-862
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• 2008

Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a zinc-dependent proteinase found in cholesterol-rich lipid rafts on the plasma membrane. MT1-MMP hydrolyzes extracellular matrix (ECM) proteins, activates pro-matrix metalloproteinase-2 (proMMP-2) and plays an important role in ECM remodeling, cancer cell migration and metastasis. The role of caveolin-1, an integral protein of caveolae, in the activation of MT1-MMP remains largely unknown. Here, we show that the expression of caveolin-1 attenuates the activation of proMMP-2, reduces proteolytic cleavage of ECM and inhibits cell migration. We utilized the cytoplasmic tail domain deletion (${\Delta}CT$) or the E240A mutant of MT1-MMP. Co-expression of caveolin-1 with the wild-type or the ${\Delta}CT$ MT1-MMP decreased the proMMP-2 activation and inhibited collagen degradation and cell migration. Caveolin-1 had no effect on the catalytically inert E240A MT1-MMP. Our findings suggest that caveolin-1 is essential in the down-regulation of MT1-MMP activity by promoting internalization from the cell surface.

• Molecules and Cells
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• v.22 no.3
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• pp.262-268
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• 2006

During endometrial differentiation the extracellular matrix (ECM) changes dramatically to prepare for implantation of the embryo. However, the genes regulating the ECM build-up in the uterine endometrium during early pregnancy are not well known. Using the PCR-select cDNA subtraction method, dermatopontin was identified in the uterus of a pregnant mouse on day 4 of gestation. Dermatopontin mRNA increased dramatically on day 3, and was at its highest level at the time of implantation. Administration of RU 486 significantly inhibited mRNA expression by day 4 of gestation, but ICI 182,780 did not. Progesterone markedly induced dermatopontin expression in ovariectomized uteri within 4 h of administration, whereas estrogen had little effect. In silico analysis revealed progesterone receptor binding sites in the dermatopontin promoter region. Decidualization did not induce expression of dermatopontin; instead dermatopontin mRNA became strongly localized at the interimplantation site. In situ hybridization revealed that expression gradually decreased in the luminal epithelial cells as pregnancy progressed, whereas it increased in the stromal cells. The pattern of localization and the changes of intensity of dermatopontin mRNA coincided with those of collagen. Collectively, these results strongly suggest that dermatopontin expression is steroid-dependent. They also suggest that, at the time of implantation, dermatopontin expression is primarily regulated spatio-temporally by progesterone via progesterone receptors, and is modulated by the decidual response during implantation. Dermatopontin may be one of the regulators used to remodel the uterine ECM for pregnancy.

• Smart Structures and Systems
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• v.14 no.2
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• pp.247-266
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• 2014

Conventional cantilevered piezoelectric energy harvesters (PEHs) are usually fabricated with continuous electrode configuration (CEC), which suffers from the electrical cancellation at higher vibration modes. Though previous research pointed out that the segmented electrode configuration (SEC) can address this issue, a comprehensive evaluation of the PEH with SEC has yet been reported. With the consideration of delivering power to a common load, the AC outputs from all segmented electrode pairs should be rectified to DC outputs separately. In such case, theoretical formulation for power estimation becomes challenging. This paper proposes a method based on equivalent circuit model (ECM) and circuit simulation to evaluate the performance of the PEH with SEC. First, the parameters of the multi-mode ECM are identified from theoretical analysis. The ECM is then established in SPICE software and validated by the theoretical model and finite element method (FEM) with resistive loads. Subsequently, the optimal performances with SEC and CEC are compared considering the practical DC interface circuit. A comprehensive evaluation of the advantageous performance with SEC is provided for the first time. The results demonstrate the feasibility of using SEC as a simple and effective means to improve the performance of a cantilevered PEH at a higher mode.

• Parasites, Hosts and Diseases
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• v.57 no.2
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• pp.101-115
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• 2019

The pathogenesis of cerebral malaria is biologically complex and involves multi-factorial mechanisms such as microvascular congestion, immunopathology by the pro-inflammatory cytokine and endothelial dysfunction. Recent data have suggested that a pleiotropic T-cell immunomodulatory protein (TIP) could effectively mediate inflammatory cytokines of mammalian immune response against acute graft-versus-host disease in animal models. In this study, we identified a conserved homologue of TIP in Plasmodium berghei (PbTIP) as a membrane protein in Plasmodium asexual stage. Compared with PBS control group, the pathology of experimental cerebral malaria (ECM) in rPbTIP intravenous injection (i.v.) group was alleviated by the downregulation of pro-inflammatory responses, and rPbTIP i.v. group elicited an expansion of regulatory T-cell response. Therefore, rPbTIP i.v. group displayed less severe brain pathology and feverish mice in rPbTIP i.v. group died from ECM. This study suggested that PbTIP may be a novel promising target to alleviate the severity of ECM.

• BMB Reports
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• v.56 no.5
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• pp.287-295
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• 2023

The tumor microenvironment (TME) is a complex system composed of many cell types and an extracellular matrix (ECM). During tumorigenesis, cancer cells constantly interact with cellular components, biochemical cues, and the ECM in the TME, all of which make the environment favorable for cancer growth. Emerging evidence has revealed the importance of substrate elasticity and biomechanical forces in tumor progression and metastasis. However, the mechanisms underlying the cell response to mechanical signals-such as extrinsic mechanical forces and forces generated within the TME-are still relatively unknown. Moreover, having a deeper understanding of the mechanisms by which cancer cells sense mechanical forces and transmit signals to the cytoplasm would substantially help develop effective strategies for cancer treatment. This review provides an overview of biomechanical forces in the TME and the intracellular signaling pathways activated by mechanical cues as well as highlights the role of mechanotransductive pathways through mechanosensors that detect the altering biomechanical forces in the TME.

• Korea Trade Review
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• v.45 no.2
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• pp.103-119
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• 2020

As the easy payment service is popularized and the competition in the service market is fierce, research on factors affecting users' continuous use of the easy payment service is becoming increasingly important. However, in the existing studies, the discussion of the continuous intention to use the consumer's simple payment service has not been sufficiently conducted. Existing research remains on the way of listing technology and consumer characteristics. To bridge this research gap, this study aims to integrate and modify ECM and UTAUT to consider factors influencing the continuous use of easy payment services. The user's expectations and the confirmation were considered as important factors to decide. For empirical analysis, a survey was conducted for 236 users who had experience with domestic easy payment service. As a result, it was verified that confirmation, performance expectancy, effort expectancy, social influence, and habit in regard to easy payment services are important for continuous use intention. Through this study, it is expected that the consumer's technology acceptance mechanism on the continuous use of simple payment will be grasped in more detail to develop academic discussions in the field. Implications from the results as well as limitations of the study are presented.