• Asian-Australasian Journal of Animal Sciences
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• 제15권4호
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• pp.500-503
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• 2002

This study was carried out to investigate the effects of season influencing semen characteristics, frozen-thawed sperm viability and testosterone concentration in Duroc boars. There were no significant differences in the semen volume and sperm concentration of Duroc boars among spring, summer, autumn and winter. However, the pH of sperm-rich and sperm-poor fractions in autumn and winter season was higher than in spring and summer season in Duroc boars. Sperm motility and normal acrosome of raw semen in Duroc boars did not differ significantly among spring, summer, autumn and winter. However, motility and normal acrosome of frozen-thawed sperm were higher in spring season than in summer, autumn and winter. Serum testosterone concentrations in Duroc were higher in spring than summer, autumn and winter. In conclusion, when serum testosterone concentrations were higher in seasons, frozen-thawed sperm viability in Duroc boars were higher.

• 한국가축번식학회지
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• 제25권1호
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• pp.79-84
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• 2001

본 연구는 Duroc 종모돈의 정액성상, 동결-융해후의 정자생존성 및 혈청 중 테스토스테론의 농도에 미치는 영향을 봄 (3~5월)과 여름 (6~8월)으로 나누어 실시한 바, 그 얻어진 결과는 다음과 같았다. 1. Duroc 종모돈의 정액량, pH, 정자농도는 봄과 여름철에 큰 차이가 없었다. 2. 봄철 및 여름철의 전체 정액량, 정자농후부분 그리고 정자희박부분에서 봄과 여름간의 차이는 나타나지 않았다. 3. 원정액 정자에서는 운동성 및 정상첨체에서 봄과 여름철간에 차이가 없었다. 그러나 동결-융해정자의 운동성 및 정상첨체는 봄철의 종모돈에서 생산한 정자가 여름철의 종모돈에서 생산한 정자보다 우수하였다. 4. 듀록 종모돈의 혈청 중 테스토스테론의 농도 변화는 봄과 여름에 각각 2.15 ng/$m\ell$과 0.65 ng/$m\ell$으로, 봄철 테스토스테론의 농도가 여름철의 농도 보다 높았다(P＜0.05). 5. 이상의 결과를 종합해 보면, 듀록 종모돈에 있어 정자의 내동성은 혈청 중 테스토스테른의 농도가 높을수록 우수한 것으로 나타났다.

• Reproductive and Developmental Biology
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• 제29권1호
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• pp.25-30
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• 2005

본 연구는 종모돈의 정액성상 동결-융해 후 정자의 생존성 그리고 혈청 중 FSH, LH, estradiol-17β 및 testosterone 농도에 미치는 품종과 계절의 영향을 조사하여 우수한 종모돈의 선발을 위한 기초자료를 얻고자 실시하였다. 요크셔종이 듀록종보다 봄, 여름, 가을, 겨울에서 정액량이 많았으며, 정액농도에서는 차이가 없었다. 계절별 정액량은 듀록 및 요크셔종에서 봄철이 여름, 가을 및 겨울철에 비하여 많았고, 정자농도는 차이가 없었다. 듀록종과 요크셔종에서 각각 봄철에 생산한 정자가 여름, 가을 및 겨울철에 생산한 정자보다 동결-융해 후 정자운동성 및 정상첨체 비율이 높았다. 한편 듀록종과 요크셔종에서 동결-융해 후 정자운동성은 모든 계절에서 요크셔종이 높게 나타났으나, 정상첨체에서는 차이가 없었다. 혈청 중 FSH의 농도를 비교한 결과 요크셔종이 듀록종보다 모든 계절에서 낮은 농도를 나타내었다. 그러나 두 품종 모두에서 각각 계절 간에 차이가 없었다. 혈청 중 LH와 estradiol-17β의 농도를 비교한 결과 요크셔종과 듀록종 간에 차이가 없었다. 또한 두 품종 모두에서 계절 간에 차이가 없었다. 종모돈의 품종별, 계절별 혈청 중 testosterone의 농도를 비교한 결과 요크셔종이 듀록종보다 모든 계절에서 높게 나타났다. 또한 두품종 모두에서 각각 봄철이 여름, 가을 및 겨울철에 비하여 혈청 중 testosterone의 농도가 높은 것으로 나타났다. 이상의 결과를 종합하여 보면, FSH의 농도가 낮을수록 정액생산량이 높은 것으로 나타났으며, 혈청 중 testosterone의 농도가 높을수록 동결-융해 정자의 운동성 및 정상첨체의 비율이 높은 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.793-798
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• 2006

The quality characteristics of semen are important indicators of the fertility of a boar. Development of genetic markers for the semen quality in boars will be beneficial to the improvement of porcine fertility. We investigated the relationship between the polymorphisms of epidermal growth factor (EGF), prostaglandin-endoperoxide synthase 2 (PTGS2) and prolactin receptor (PRLR) genes, and semen quality traits in boars. The genomic DNA of 233 boars (157 Duroc and 86 Landrace) from a central testing station was subjected to genotyping for surveying gene frequency. The EGF, PTGS2 and PRLR genotypes were determined using the restriction fragment length polymorphism method. Thirty-seven normal, mature Duroc boars from an AI center were also genotyped and their semen quality traits were collected. The effect of genotype on semen quality traits was analyzed by the least-squares means method using data corrected for season. The frequencies of the AA genotype of EGF, PTGS2 and PRLR in Duroc boars were 0.14, 0.01 and 0.66, respectively. In Landrace, the frequencies of the AA genotype were 0.03, 0.09 and 0.62, respectively. Boars with the BB genotype in EGF, with the AB genotype in PTGS2 and with the AA genotype in PRLR had significantly better semen quality with a higher percentage of normal sperm and a lower percentage of immature sperm than those with other genotypes. These findings imply that polymorphisms of EGF, PTGS2 and PRLR genes might be used as markers for improving the semen quality of boars.

• 한국수정란이식학회지
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• 제9권1호
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• pp.49-52
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• 1994

This study was carried out to compare farrowing rate and litter traits for European and American lines with boar sperm frozen in straws. Farrowing rate, litter size and mean pig weght at birth and 21 days were investigated. A total of 36 gilts Landrace, Large white and Duroc were investigated at the Chungnam Provincial Animal Breeding Station. We obtained higher farrowing rate and litter traits for European line boars compared to American line boars.

• Asian-Australasian Journal of Animal Sciences
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• 제18권1호
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• pp.96-101
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• 2005

There were two trials involved in the experiment. Trial 1: the trial was conducted on two Taiwan Sugar Corporation (TSC) pig farms. One was located in the north of Taiwan and the other was located in the south. Both farms had wet pad and forced ventilation (WPFV) and conventional open design (COD) boar and sow houses. There were 12 Duroc boars, age ranging from 12-24 months. Half of them (6 boars) were raised in a WPFV pig house, and the other half were kept in a COD house. Semen was collected at 5-day intervals from May $1^{st}$ to the end of October. Sixteen sows (2-8 parity) were served by artificial insemination each week from the beginning of May to the end of Oct. These sows were checked for heat from 18 days to 25 days after insemination. Trial 2: there were four MPFV boar houses involved in the test. Two houses were located in the north of Taiwan, and the other two houses were located in the south. The test was conducted from January 2000 to December 2001. The total number of serviced sows by MPFV-housed boars was 35,105 head and for COD-housed boars 103,065 head. The results showed that the total semen volume, density of sperm, total sperm per ejaculate, sperm motility and morphological abnormality were significantly better (p<0.01) for boar raised in WPFV house than for COD houses. Average sperm motility in June and July was lower than for the other months. Morphological abnormality was higher during May, June and July. Although the results did not reach a significant level, the average value showed that the total volume of boar semen was higher in the north than for the south. The total semen volume production of boar raised in WPFV was higher than for boars raised in COD house, reaching a significant level only in summer. Boars kept in WPFV house had higher total sperm number than boars kept in COD house, reaching a significant level in spring (p<0.05), summer (p<0.01), and fall (p<0.05) but not in winter (p>0.05). Boars raised in WPFV house had significantly higher sperm motility than boars in COD house during spring (p<0.001), summer (p<0.001), fall (p<0.01) and winter (p<0.05). The average farrowing rate and piglets born alive were higher for boars in WPFV house than for boars in COD house, but neither reached a significant level (p>0.05). The present experiment shows that WPFV house can improve the reproduction performance of boars.

• Asian-Australasian Journal of Animal Sciences
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• 제4권2호
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• pp.143-150
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• 1991

The data on reproductive performance of 1065 purebred Landrace, 1254 purebred Yorkshire. 553 purebred Duroc and 327 purebred Pietrain sows bred to farrow purebred litters and data from 3413 Landrace $\times$ Yorkshire, 487 Duroc $\times$ Pietrain and 3191 commericial litters were udsed in this study. The data were collected form litters farrowed form January 1986 to December 1988 in Holiday Hills Stock and Breeding Farms, San Prero, Laguna. The crossbred sows and litters were better in most traits associated with sow productivity than their contemporary purebred sows and litters. The Duroc $\times$ Pietrain crossbred litters showed 16.67% improvement in litter size at weaning, 10.11% improvement in survival rate form birth to weaning and 16.21% more pigs per sow pre year than the averages of their parental breeds. The litters from the Landrace $\times$ Yorkshire crossbred sows sired by either Duroc or Duroc $\times$ Pietrain boars had 11.01% bigger litter size at birth, 20.74% increase in litter size at weaning and 22.86% more pigs weaned per sow per year than the averages of the four parental breeds. Very little improvement in most traits evaluated were observed form the Landrace $\times$ Yorkshire crossbred litters compared with their parental breeds. Year of birth of litter had litter influence on sow productivity. However, the reproductivity performance declined when sows farrowed in June up to September. The best Months of farrowing were found to be from January to April. The breed $\times$ year, breed $\times$ month and year $\times$ month had no significant effects on all traits evaluated.

• Journal of Animal Science and Technology
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• 제49권2호
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• pp.257-268
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• 2007

본 연구는 멧돼지 교잡종육과 재래 흑돼지육, 개량종 돼지육의 냉장저장중 품질을 비교하고자 실시하였다. 방목사육된 멧돼지 교잡종(멧돼지 ♂×Duroc ♀, 평균 113kg, 거세돈 1두 및 미경산돈 3두) 4두와 옥내사육된 재래 흑돼지 5두(평균 64kg, 거세돈 5두) 및 개량종 돼지 5두(Landrace×Yorkshire×Duroc, 평균 114kg, 거세돈 5두)를 도축한 다음 등심(M. longssimus) 부위를 2±0.2℃에서 12일 동안 저장하면서 품질분석에 이용하였다. 수분 함량은 멧돼지 교잡종육이 재래 흑돼지육보다 높았으나(p<0.05), 조지방 함량은 낮았다(p<0.05). pH는 멧돼지 교잡종육이 저장기간 동안 개량종 돼지육보다 낮았으며(p<0.05), 그에 따라 낮은 보수력을 보였다(p<0.05). 표면육색은 멧돼지 교잡종육의 L*, a*, b*, C* 값이 저장기간 동안 재래 흑돼지육보다 낮았던 반면(p<0.05), 저장 3, 6일부터는 개량종 돼지육보다 높았다(p<0.05). 지방산 조성은 멧돼지 교잡종육이 타품종 돈육에 비해 포화지방산 함량이 낮고(p<0.05), linoleic acid와 arachidonic acid를 포함한 다가불포화지방산이 높았음에도 불구하고 저장기간 동안 지방산화는 지연되었다. 전자코의 PCA에 의한 향기패턴은 저장 0, 12일에 세 품종의 돈육간에 뚜렷한 차이를 보였다.

• Reproductive and Developmental Biology
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• 제29권3호
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• pp.149-154
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• 2005

The purpose of this study was the analysis of sperm ability in Specific Pathogen Free (SPE) miniature pig for production of bio-organ. The collected semen was diluted with extender and stored at $17^{\circ}C$t for up to 7 days. The semen samples were evaluated at 0, 1, 3, 5, and 7 days of storage for analysis of sperm ability. Sperm ability was evaluated by examining viability, progressive motility, sperm abnormality and intensity of the sperm membrane. Also, the semen was processed according to the convenient freezing method, and frozen-thawed sperm was evaluated by examining viability, capacitation and acrosome reaction using chlortetracycline (CTC) staining. Motility of spermatozoa of SPF miniature pig was significantly (P<0.05) lower on 3 days or later compared to the Duroc, Yorkshire and Landrace in domestic boar. The percentage of abnormal spermatozoa of Landrace were significantly (P<0.05) higher than in SPF miniature pig, Duroc and Yorkshire that had a similar percentage on 5 or 7 days of sperm storage. The percentage of spermatozoa with coiled tail decreased during the storage period but there were no significant difference. On the other hand, viability of frozen-thawed spermatozoa had a significantly (P<0.05) lower in SPF miniature pig than in other domestic boars. CTC patterns had no significant difference, but SPF miniature pig had higher percentage of capacitated spermatozoa and lower percentage of acrosome-reacted it than domestic boars. Therefore, this study suggest that it is necessary to develop the suitable extender and freezing methods methods for the high viable rate and fertilizing ability in vitro.

• Journal of Animal Science and Technology
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• 제50권1호
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• pp.121-132
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• 2008

[Landrace×Yorkshire]×Duroc(LYD) 및 [Yorkshire×Berkshire]×Berkshire(YBB) 삼원교잡종과 흑색계 영국 Berkshire(BB; British berkshire), 가고시마 Berkshire(KB; Kagoshima Berkshire), [재래돼지×멧돼지(KNW; Native black pig×Wild boars)] 이원교잡종에서 생산된 돼지 100두를 생체중 110 kg 도달시 도축한 후 등심을 채취하여 분석하였다. 수분 함량은 KW, 조단백질은 YBB와 KW, 콜레스테롤 함량은 YBB가 다른 품종들보다 유의적으로 높았다(p<0.05). 다른 품종들에 비해 YBB는 보수력과 pH는 낮고, 전단가는 높았다(p<0.05). LYD에서 명도(L*)가 유의적으로 높게 나타났으며(p<0.05), 적색도(a*)와 파쇄성에서는 품종들 간에 유의적인 차이가 없었다(p>0.05). UFA/SFA와 EFA/UFA 비율과 EAA은 KW가 높게 나타났으나(p<0.05) SAAA는 유의적으로 낮았다(p<0.05). 총 아미노산 함량은 LYD와 BB가 각각 20.44%, 20.81%로 다른 품종에 비해 유의적으로 많았다(p<0.05). 신선육 상태에서 품종은 육색, 드립로스, 마블링과 전체적인 기호도에 영향을 미쳤지만(p<0.05), 조리육의 경우 풍미를 제외한 관능검사 항목에는 영향을 미치지 않았다(p>0.05).