• 제목/요약/키워드: Delta sequence

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Production of Gamma-Linolenic Acid in Pichia pastoris by Expression of a Delta-6 Desaturase Gene from Cunninghamella echinulata

  • Wan, Xia;Zhang, Yinbo;Wang, Ping;Huang, Fenghong;Chen, Hong;Jiang, Mulan
    • Journal of Microbiology and Biotechnology
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    • 제19권10호
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    • pp.1098-1102
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    • 2009
  • Gamma-linolenic acid (GLA, C18:3 ${\Delta}^{6,9,12}$) is synthesized by a delta-6 fatty acid desaturase using linoleic acid (LA, C18:2 ${\Delta}^{9,12}$) as a substrate. To enable the production of GLA in the conventional yeast Pichia pastoris, we have isolated a cDNA encoding the delta-6 fatty acid desaturase from Cunninghamella echinulata MIAN6 and confirmed its function by heterogeneous expression in P. pastoris. Sequence analysis indicated that this cDNA sequence has an open reading frame of 1,404 bp, which encodes a 52 kDa peptide of 468 amino acids. This sequence has 64% identity to the previously reported delta-6 fatty acid desaturase from Rhizopus oryzae. The polypeptide has a cytochrome b5 domain at the N-terminus including the HPGG motif in the heme-binding region, as reported for other delta-6 fatty acid desaturases. In addition, this enzyme differs from other desaturases by the presence of three possible N-linked glycosylation sites. Analysis of the fatty acid composition demonstrated the accumulation of GLA to the level of 3.1% of the total fatty acids. Notably, the amounts of ginkgolic acid (C17:1) and palmitic acid (C16:0) were increased from 1.3% to 29.6% and from 15% to 33%, respectively. These results reveal that the modification of the fatty acid biosynthetic pathway by genetic manipulation in order to produce specific polyunsaturated fatty acids in P. pastoris is a promising technique.

상관 함수의 기울기 차에 기반한 GNSS의 부호 추적 기법 (A GNSS Code Tracking Scheme Based in Slope Difference of Correlation Outputs)

  • 유승수;유승환;정다해;안상호;윤석호;김선용
    • 한국통신학회논문지
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    • 제33권6C호
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    • pp.505-511
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    • 2008
  • 위성 항법 시스템은 (global navigation satellite system, GNSS) 통신 물리계층으로 직접 수열 확산 대역 (direct sequence/spread spectrum, DS/SS) 시스템을 사용하고 있다. DS/SS 시스템은 확산된 정보를 복원하기 위해 한 칩 (chip) 또는 그 이하로 동기를 맞추는 획득 단계와 정확한 동기를 결정하고 유지하는 추적 단계를 수행한다. 가장 널리 알려진 추적 기법은 상관 값의 대칭성을 이용해 부호를 추적하는 $\Delta$-DLL이다 (single delta delay lock loop). 여기서, $\Delta$는 이른-늦은 상관 값의 상관 시간 옵셋 간격을 뜻한다. $\Delta$-DLL은 이상적인 환경에서 최적 부호 추적 기법이다. 그러나 이 기법은 다중경로 환경에서 상관 함수의 대칭성이 깨짐으로 인해 판별기 출력이 비대칭으로 나타나기 때문에 큰 추적 편이를 갖는다. 추적 편이는 정착한 동기 시점과 추적이 완료되어 결정한 동기 시점의 차이를 의미한다. 이러한 추적 편이를 감소시키기 위한 기법으로 작은 $\Delta$를 사용한 $\Delta$-DLL과 두 개의 $\Delta$-DLL을 조합한 ${\Delta}^{(2)}$-DLL이 (double delta DLL) 제안되었다. 그러나 두 기법 모두 여전히 추적 편이가 존재하며, 작은 $\Delta$를 사용하기 때문에 추적 단계의 동적 영역이 줄어들어 정확한 획득 단계가 요구되는 단점을 갖고 있다. 본 논문에서는 추적 편이를 효과적으로 줄이면서도 정확한 획득 단계가 요구되지 않는 상관 값의 최대 기울기 변화에 기반한 추적 편이 감소 기법을 제안한다.

Some Difference Paranormed Sequence Spaces over n-normed Spaces Defined by a Musielak-Orlicz Function

  • Raj, Kuldip;Sharma, Sunil K.;Gupta, Amit
    • Kyungpook Mathematical Journal
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    • 제54권1호
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    • pp.73-86
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    • 2014
  • In the present paper we introduce difference paranormed sequence spaces $c_0(\mathcal{M},{\Delta}^n_m,p,u,{\parallel}{\cdot},{\cdots},{\cdot}{\parallel})$, $c(\mathcal{M},{\Delta}^n_m,p,u,{\parallel}{\cdot},{\cdots},{\cdot}{\parallel})$ and $l_{\infty}(\mathcal{M},{\Delta}^n_m,p,u,{\parallel}{\cdot},{\cdots},{\cdot}{\parallel})$ defined by a Musielak-Orlicz function $\mathcal{M}$ = $(M_k)$ over n-normed spaces. We also study some topological properties and some inclusion relations between these spaces.

Molecular Cloning, Characterization, and Expression Analysis of Chicken Δ-6 Desaturase

  • Kang, Xiangtao;Bai, Yichun;Sun, Guirong;Huang, Yanqun;Chen, Qixin;Han, Ruili;Li, Guoxi;Li, Fadi
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권1호
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    • pp.116-121
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    • 2010
  • Long-chain polyunsaturated fatty acids (LC-PUFA) promote the development of brain and vision of the fetus, relieve inflammation, inhibit oral dysplasia of rumor cell, decrease the incidence of cardiovascular disease and regulate arrhythmia. ${\Delta}-6$ desaturase is the rate-limited enzyme in the desaturation process. This study reports the cloning, characterization and tissue expression of a ${\Delta}-6$ desaturase gene in the chicken. PCR primers were designed based on the predicted sequence of chicken ${\Delta}-6$ desaturase (accession number: XM421053) and used to isolate a cDNA fragment of 1,323 bp from chicken liver. Based on the 1,323 bp fragment an EST (BI390105) was obtained by BLAST. The EST and 5'nd of the 1,323 bp fragment were partially overlapped. Gene specific primers derived from the EST were used for amplification of the 5'nd. Another gene-specific primer derived from the 1,323 bp fragment was used for amplification of the 3'nd by 3'ACE. Then the three overlapping cDNA sequences obtained were assembled with DNAMAN software and a full-length ${\Delta}-6$ desaturase of 2,153 bp was obtained. The full-length cDNA contained an ORF of 1,335 bp with a 5'ntranslated region of 147 nucleotides followed by an ATG initiation codon. Stop codon TGA was at position 1,481-1,483 bp. The deduced amino acids shared an homology above 77% with bovine, mice, orangutan, rat and human. The protein sequence had three histidine-rich regions HDFGH (HisI region), HFQHH (HisII region) and HH (HisIII region), a cytochrome $b_{5}$-like domain containing a heme-binding motif and two transmembrane domains. Sequence analysis of the chicken genomic DNA revealed that the coding sequence of chicken ${\Delta}-6$ desaturase included 12 exons and 11 introns. Semi-quantitative RT-PCR showed that the ${\Delta}-6$ desaturase expression levels were in turn liver, spleen, pancreas, lung, breast muscle, heart, and abdominal fat. The expression of ${\Delta}-6$ desaturase in liver was significantly higher than that in breast muscle (p<0.01). The expression of ${\Delta}-6$ desaturase in lung was significantly higher than that in abdominal fat (p<0.01). This is the first clone of chicken ${\Delta}-6$ desaturase.

Calibrations of Ultraviolet Excess as Metallicity Indicator of F,G,K Stars

  • Ann, Hong-Bae
    • 천문학논총
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    • 제1권1호
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    • pp.39-54
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    • 1984
  • For F,G,K stars, their photometric data and metallicity, [Fe/H] are collected and their correlations between $\delta$(U-B) and [Fe/H] for each spectral group are examined, using a reference sequence which is defined by the stars in Woolley's catalogue and other reference sequences. Detailed examination shows that the reference sequence by Woolley's catalogue appears to be properly defined for the calculation of $\delta$(U-B) of population I stars in the solar neighborhood. It is suggested that accuracies of metallicities derived from $\delta$(U-B) can be improved if we derive the correlations between [Fe/H] and $\delta$(U-B) from the stars in each spectral group.

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Sequence Dependent Binding Modes of the ΔΔ- and ΛΛ-binuclear Ru(II) Complexes to poly[d(G-C)2] and poly[d(A-T)2]

  • Chitrapriya, Nataraj;Kim, Raeyeong;Jang, Yoon Jung;Cho, Dae Won;Han, Sung Wook;Kim, Seog K.
    • Bulletin of the Korean Chemical Society
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    • 제34권7호
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    • pp.2117-2124
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    • 2013
  • The binding properties and sequence selectivities of ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ (bip = 4,4'-biphenylene (imidazo [4,4-f][1,10]phenanthroline) complexes with $poly[d(A-T)_2]$ and $poly[d(G-C)_2]$ were investigated using conventional spectroscopic methods. When bound to $poly[d(A-T)_2]$, a large positive circular dichroism (CD) spectrum was induced in absorption region of the bridging moiety for both the ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complexes, which suggested that the bridging moiety sits in the minor groove of the polynucleotide. As luminescence intensity increased, decay times became longer and complexes were well-protected from the negatively charged iodide quencher compared to that in the absence of $poly[d(A-T)_2]$. These luminescence measurements indicated that Ru(II) enantiomers were in a less polar environment compared to that in water and supported by minor groove binding. An angle of $45^{\circ}$ between the molecular plane of the bridging moiety of the ${\Delta}{\Delta}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complex and the local DNA helix axis calculated from reduced linear dichroism ($LD^r$) spectrum further supported the minor groove binding mode. In the case of ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complex, this angle was $55^{\circ}$, suggesting a tilt of DNA stem near the binding site and bridging moiety sit in the minor groove of the $poly[d(A-T)_2]$. In contrast, neither ${\Delta}{\Delta}$-nor ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complex produced significant CD or $LD^r$ signal in the absorption region of the bridging moiety. Luminescence measurements revealed that both the ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complexes were partially accessible to the $I^-$ quencher. Furthermore, decay times became shorter when bis-Ru(II) complexes bound to $poly[d(G-C)_2]$. These observations suggest that both the ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complexes bind at the surface of $poly[d(G-C)_2]$, probably electrostatically to phosphate group. The results indicate that ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ are able to discriminate between AT and GC base pairs.

On Generalized Absolute Riesz Summability Factor of Infinite Series

  • Sonker, Smita;Munjal, Alka
    • Kyungpook Mathematical Journal
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    • 제58권1호
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    • pp.37-46
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    • 2018
  • The objective of the present manuscript is to obtain a moderated theorem proceeding with absolute Riesz summability ${\mid}{\bar{N}},p_n,{\gamma};{\delta}{\mid}_k$ by applying almost increasing sequence for infinite series. Also, a set of reduced and well-known factor theorems have been obtained under suitable conditions.

Analysis of Delta-V Losses During Lunar Capture Sequence Using Finite Thrust

  • Song, Young-Joo;Park, Sang-Young;Kim, Hae-Dong;Lee, Joo-Hee;Sim, Eun-Sup
    • Journal of Astronomy and Space Sciences
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    • 제28권3호
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    • pp.203-216
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    • 2011
  • To prepare for a future Korean lunar orbiter mission, semi-optimal lunar capture orbits using finite thrust are designed and analyzed. Finite burn delta-V losses during lunar capture sequence are also analyzed by comparing those with values derived with impulsive thrusts in previous research. To design a hypothetical lunar capture sequence, two different intermediate capture orbits having orbital periods of about 12 hours and 3.5 hours are assumed, and final mission operation orbit around the Moon is assumed to be 100 km altitude with 90 degree of inclination. For the performance of the on-board thruster, three different performances (150 N with $I_{sp}$ of 200 seconds, 300 N with $I_{sp}$ of 250 seconds, 450 N with $I_{sp}$ of 300 seconds) are assumed, to provide a broad range of estimates of delta-V losses. As expected, it is found that the finite burn-arc sweeps almost symmetric orbital portions with respect to the perilune vector to minimize the delta-Vs required to achieve the final orbit. In addition, a difference of up to about 2% delta-V can occur during the lunar capture sequences with the use of assumed engine configurations, compared to scenarios with impulsive thrust. However, these delta-V losses will differ for every assumed lunar explorer's on-board thrust capability. Therefore, at the early stage of mission planning, careful consideration must be made while estimating mission budgets, particularly if the preliminary mission studies were assumed using impulsive thrust. The results provided in this paper are expected to lead to further progress in the design field of Korea's lunar orbiter mission, particularly the lunar capture sequences using finite thrust.