• Title/Summary/Keyword: Delta sequence

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Production of Gamma-Linolenic Acid in Pichia pastoris by Expression of a Delta-6 Desaturase Gene from Cunninghamella echinulata

  • Wan, Xia;Zhang, Yinbo;Wang, Ping;Huang, Fenghong;Chen, Hong;Jiang, Mulan
    • Journal of Microbiology and Biotechnology
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    • v.19 no.10
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    • pp.1098-1102
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    • 2009
  • Gamma-linolenic acid (GLA, C18:3 ${\Delta}^{6,9,12}$) is synthesized by a delta-6 fatty acid desaturase using linoleic acid (LA, C18:2 ${\Delta}^{9,12}$) as a substrate. To enable the production of GLA in the conventional yeast Pichia pastoris, we have isolated a cDNA encoding the delta-6 fatty acid desaturase from Cunninghamella echinulata MIAN6 and confirmed its function by heterogeneous expression in P. pastoris. Sequence analysis indicated that this cDNA sequence has an open reading frame of 1,404 bp, which encodes a 52 kDa peptide of 468 amino acids. This sequence has 64% identity to the previously reported delta-6 fatty acid desaturase from Rhizopus oryzae. The polypeptide has a cytochrome b5 domain at the N-terminus including the HPGG motif in the heme-binding region, as reported for other delta-6 fatty acid desaturases. In addition, this enzyme differs from other desaturases by the presence of three possible N-linked glycosylation sites. Analysis of the fatty acid composition demonstrated the accumulation of GLA to the level of 3.1% of the total fatty acids. Notably, the amounts of ginkgolic acid (C17:1) and palmitic acid (C16:0) were increased from 1.3% to 29.6% and from 15% to 33%, respectively. These results reveal that the modification of the fatty acid biosynthetic pathway by genetic manipulation in order to produce specific polyunsaturated fatty acids in P. pastoris is a promising technique.

A GNSS Code Tracking Scheme Based in Slope Difference of Correlation Outputs (상관 함수의 기울기 차에 기반한 GNSS의 부호 추적 기법)

  • Yoo, Seung-Soo;Yoo, Seung-Hwan;Chong, Da-Hae;Ahn, Sang-Ho;Yoon, Seok-Ho;Kim, Sun-Yong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.6C
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    • pp.505-511
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    • 2008
  • The global navigation satellite system (GNSS) is using a direct sequence/spread spectrum (DS/SS) modulation. In order to recover the information data, the DS/SS system first performs a two-step synchronization process: acquisition and tracking. The acquisition process adjusts the phase difference between the received and locally generated acquisition sequences within ${\pm}T_c/2$ or less, where $T_c$ is the chip period. The tracking process performs fine synchronization. In this paper, we focus on the tracking issue. The single delta delay locked loop($\Delta$-DLL) is the optimal tracking scheme for a GNSS in the absence of multipath signals, where $\Delta$ means the spacing between the early and late correlation time offset. In the multipath environments, however, the $\Delta$-DLL suffers from huge estimation bias(denoted by $\beta$) caused by distorted correlation values. Although some modified schemes such as a $\Delta$-DLL with a narrow $\Delta$ and a double delta DLL (${\Delta}^{(2)}$-DLL) were proposed to reduce the estimation bias, they cannot remove the estimation bias completely and need more accurate acquisition process. This paper proposes a novel tracking scheme that can dramatically reduce the estimation bias, using the maximum slope change among the correlation outputs.

Some Difference Paranormed Sequence Spaces over n-normed Spaces Defined by a Musielak-Orlicz Function

  • Raj, Kuldip;Sharma, Sunil K.;Gupta, Amit
    • Kyungpook Mathematical Journal
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    • v.54 no.1
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    • pp.73-86
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    • 2014
  • In the present paper we introduce difference paranormed sequence spaces $c_0(\mathcal{M},{\Delta}^n_m,p,u,{\parallel}{\cdot},{\cdots},{\cdot}{\parallel})$, $c(\mathcal{M},{\Delta}^n_m,p,u,{\parallel}{\cdot},{\cdots},{\cdot}{\parallel})$ and $l_{\infty}(\mathcal{M},{\Delta}^n_m,p,u,{\parallel}{\cdot},{\cdots},{\cdot}{\parallel})$ defined by a Musielak-Orlicz function $\mathcal{M}$ = $(M_k)$ over n-normed spaces. We also study some topological properties and some inclusion relations between these spaces.

Molecular Cloning, Characterization, and Expression Analysis of Chicken Δ-6 Desaturase

  • Kang, Xiangtao;Bai, Yichun;Sun, Guirong;Huang, Yanqun;Chen, Qixin;Han, Ruili;Li, Guoxi;Li, Fadi
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.1
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    • pp.116-121
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    • 2010
  • Long-chain polyunsaturated fatty acids (LC-PUFA) promote the development of brain and vision of the fetus, relieve inflammation, inhibit oral dysplasia of rumor cell, decrease the incidence of cardiovascular disease and regulate arrhythmia. ${\Delta}-6$ desaturase is the rate-limited enzyme in the desaturation process. This study reports the cloning, characterization and tissue expression of a ${\Delta}-6$ desaturase gene in the chicken. PCR primers were designed based on the predicted sequence of chicken ${\Delta}-6$ desaturase (accession number: XM421053) and used to isolate a cDNA fragment of 1,323 bp from chicken liver. Based on the 1,323 bp fragment an EST (BI390105) was obtained by BLAST. The EST and 5'nd of the 1,323 bp fragment were partially overlapped. Gene specific primers derived from the EST were used for amplification of the 5'nd. Another gene-specific primer derived from the 1,323 bp fragment was used for amplification of the 3'nd by 3'ACE. Then the three overlapping cDNA sequences obtained were assembled with DNAMAN software and a full-length ${\Delta}-6$ desaturase of 2,153 bp was obtained. The full-length cDNA contained an ORF of 1,335 bp with a 5'ntranslated region of 147 nucleotides followed by an ATG initiation codon. Stop codon TGA was at position 1,481-1,483 bp. The deduced amino acids shared an homology above 77% with bovine, mice, orangutan, rat and human. The protein sequence had three histidine-rich regions HDFGH (HisI region), HFQHH (HisII region) and HH (HisIII region), a cytochrome $b_{5}$-like domain containing a heme-binding motif and two transmembrane domains. Sequence analysis of the chicken genomic DNA revealed that the coding sequence of chicken ${\Delta}-6$ desaturase included 12 exons and 11 introns. Semi-quantitative RT-PCR showed that the ${\Delta}-6$ desaturase expression levels were in turn liver, spleen, pancreas, lung, breast muscle, heart, and abdominal fat. The expression of ${\Delta}-6$ desaturase in liver was significantly higher than that in breast muscle (p<0.01). The expression of ${\Delta}-6$ desaturase in lung was significantly higher than that in abdominal fat (p<0.01). This is the first clone of chicken ${\Delta}-6$ desaturase.

Calibrations of Ultraviolet Excess as Metallicity Indicator of F,G,K Stars

  • Ann, Hong-Bae
    • Publications of The Korean Astronomical Society
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    • v.1 no.1
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    • pp.39-54
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    • 1984
  • For F,G,K stars, their photometric data and metallicity, [Fe/H] are collected and their correlations between $\delta$(U-B) and [Fe/H] for each spectral group are examined, using a reference sequence which is defined by the stars in Woolley's catalogue and other reference sequences. Detailed examination shows that the reference sequence by Woolley's catalogue appears to be properly defined for the calculation of $\delta$(U-B) of population I stars in the solar neighborhood. It is suggested that accuracies of metallicities derived from $\delta$(U-B) can be improved if we derive the correlations between [Fe/H] and $\delta$(U-B) from the stars in each spectral group.

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Sequence Dependent Binding Modes of the ΔΔ- and ΛΛ-binuclear Ru(II) Complexes to poly[d(G-C)2] and poly[d(A-T)2]

  • Chitrapriya, Nataraj;Kim, Raeyeong;Jang, Yoon Jung;Cho, Dae Won;Han, Sung Wook;Kim, Seog K.
    • Bulletin of the Korean Chemical Society
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    • v.34 no.7
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    • pp.2117-2124
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    • 2013
  • The binding properties and sequence selectivities of ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ (bip = 4,4'-biphenylene (imidazo [4,4-f][1,10]phenanthroline) complexes with $poly[d(A-T)_2]$ and $poly[d(G-C)_2]$ were investigated using conventional spectroscopic methods. When bound to $poly[d(A-T)_2]$, a large positive circular dichroism (CD) spectrum was induced in absorption region of the bridging moiety for both the ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complexes, which suggested that the bridging moiety sits in the minor groove of the polynucleotide. As luminescence intensity increased, decay times became longer and complexes were well-protected from the negatively charged iodide quencher compared to that in the absence of $poly[d(A-T)_2]$. These luminescence measurements indicated that Ru(II) enantiomers were in a less polar environment compared to that in water and supported by minor groove binding. An angle of $45^{\circ}$ between the molecular plane of the bridging moiety of the ${\Delta}{\Delta}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complex and the local DNA helix axis calculated from reduced linear dichroism ($LD^r$) spectrum further supported the minor groove binding mode. In the case of ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complex, this angle was $55^{\circ}$, suggesting a tilt of DNA stem near the binding site and bridging moiety sit in the minor groove of the $poly[d(A-T)_2]$. In contrast, neither ${\Delta}{\Delta}$-nor ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complex produced significant CD or $LD^r$ signal in the absorption region of the bridging moiety. Luminescence measurements revealed that both the ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complexes were partially accessible to the $I^-$ quencher. Furthermore, decay times became shorter when bis-Ru(II) complexes bound to $poly[d(G-C)_2]$. These observations suggest that both the ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ complexes bind at the surface of $poly[d(G-C)_2]$, probably electrostatically to phosphate group. The results indicate that ${\Delta}{\Delta}$- and ${\Lambda}{\Lambda}-[{\mu}-Ru_2(phen)_4(bip)]^{4+}$ are able to discriminate between AT and GC base pairs.

On Generalized Absolute Riesz Summability Factor of Infinite Series

  • Sonker, Smita;Munjal, Alka
    • Kyungpook Mathematical Journal
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    • v.58 no.1
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    • pp.37-46
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    • 2018
  • The objective of the present manuscript is to obtain a moderated theorem proceeding with absolute Riesz summability ${\mid}{\bar{N}},p_n,{\gamma};{\delta}{\mid}_k$ by applying almost increasing sequence for infinite series. Also, a set of reduced and well-known factor theorems have been obtained under suitable conditions.

Analysis of Delta-V Losses During Lunar Capture Sequence Using Finite Thrust

  • Song, Young-Joo;Park, Sang-Young;Kim, Hae-Dong;Lee, Joo-Hee;Sim, Eun-Sup
    • Journal of Astronomy and Space Sciences
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    • v.28 no.3
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    • pp.203-216
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    • 2011
  • To prepare for a future Korean lunar orbiter mission, semi-optimal lunar capture orbits using finite thrust are designed and analyzed. Finite burn delta-V losses during lunar capture sequence are also analyzed by comparing those with values derived with impulsive thrusts in previous research. To design a hypothetical lunar capture sequence, two different intermediate capture orbits having orbital periods of about 12 hours and 3.5 hours are assumed, and final mission operation orbit around the Moon is assumed to be 100 km altitude with 90 degree of inclination. For the performance of the on-board thruster, three different performances (150 N with $I_{sp}$ of 200 seconds, 300 N with $I_{sp}$ of 250 seconds, 450 N with $I_{sp}$ of 300 seconds) are assumed, to provide a broad range of estimates of delta-V losses. As expected, it is found that the finite burn-arc sweeps almost symmetric orbital portions with respect to the perilune vector to minimize the delta-Vs required to achieve the final orbit. In addition, a difference of up to about 2% delta-V can occur during the lunar capture sequences with the use of assumed engine configurations, compared to scenarios with impulsive thrust. However, these delta-V losses will differ for every assumed lunar explorer's on-board thrust capability. Therefore, at the early stage of mission planning, careful consideration must be made while estimating mission budgets, particularly if the preliminary mission studies were assumed using impulsive thrust. The results provided in this paper are expected to lead to further progress in the design field of Korea's lunar orbiter mission, particularly the lunar capture sequences using finite thrust.