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Protease Activities in Tenderizing Effect of Vegetables used as Cooking Material (조리용 채소의 단백분해효소 활성 및 연육효과)

  • Suh, Hyung-Joo;Chung, Soo-Hyun;Choi, Yang-Mun;Cho, Won-Dai
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.883-887
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    • 1998
  • Protease activities were measured in vegetables used as cooking material or a side dish. Proteases of green pepper (Kwari and Chungyang), perilla leaves, soybean sprout and mungbean sprout were showed high activities. Especially, protease in soybean sprout was the highest activity among them. After dialysis, remaining activities in mungbean sprout, green pepper (Kwari and Chungyang) and perilla leaves were 12, 23, 45% and 37%. In the results of thawing after freezing the proteases, remaining activities in sprout of mungbean and soybean were 100% and 65%. Protease in soybean sprout was showed higher activity and stability than others. Proteolytic effects of soybean sprout on myofibrillar and sarcoplasmic protein were showed higher than stroma protein. In SDS-PAGE, myosin heavy chain, actin and tropomyosin were hydrolyzed with increasing time. Protease activity was rapidly increased with increasing meat concentration in a early proteolysis reaction, but was slightly increased in later.

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Development of the Automatic Watering System for the Soybean Sprouting Equipment by Siphon and Well Bucket (싸이펀과 두레박을 이용한 콩나물 재배기용 자동 수주 시스템 개발)

  • Kim, Joong-Man;Choi, Yong-Bae;Hwang, Ho-Sun;Yang, Dai-Ki;Jeon, Ye-Jung;Song, Young-Ae
    • Korean Journal of Food Science and Technology
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    • v.34 no.3
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    • pp.533-535
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    • 2002
  • The new watering system for the cabinet type of soybean sprouting equipment was developed. It was made of one siphon $({\Phi}35 mm)$, one well bucket $(13{\times}10{\times}10\;cm)$ that its weight-center is changed by water level, and bottle-neck water tank (bottom: $18.5^W{\times}32^L{\times}29^H\;cm$, top: $14^W{\times}14^L{\times}25^H\;cm)$. The watering system can be automatically watered more than 750 mL/sec without electric supply. The soybean sprouting equipment is consisted of the watering system and nine sprouting buckets. In addition siphon phenomena brought about easily by narrowing same as bottle-neck shape top portion of water tank that inserted a siphon. The equipment with the watering system may be produced more than three box a day in the condition of $25{\pm}1^{\circ}C$, and $6{\sim}7$ watering times a day.

An Study on the Construction of Basic Data System for Restoration of 3x3 Kan Central Hall Remains of Buddhist Temple in Joseon Dynasty - Comparison with the floor plan of the extant Hall- (조선시대 사찰의 3X3칸 주불전 건물지 복원을 위한 자료구축에 관한 기초연구 - 현존 주불전과의 평면 계획 비교를 중심으로-)

  • Kim, Hwan-Chol;An, Dai-Whan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.9
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    • pp.163-177
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    • 2016
  • This study suggests a method for making a system of basic materials for the restoration of the 3X3 Kan central hall remains of a Buddhist temple from the Joseon Dynasty. Because there are both an extant hall and remains from the Joseon Dynasty, the basic material can be constructed through comparison with the floor plan. Multi-step comparisons were found to be the most suitable example for restoration. For this study, five parts of the 3x3 Kan remains were compared with 70 extant halls for a case study. For the first comparison, the limit of collection makes four examples in the whole examples. There are many relationships with the area and the length ratio of the front to the side as the comparison articles, so there are examples that satisfied both comparison articles. However, there is little relationship between the length ratio of the front Eokan to the front Hyeopkan and the length ratio of side Eokan to the side Hyeopkan. The construction method of the basic material shows the possibility of expansion with various directions that have pros and cons.

Immunoregulatory Effects of Saengshik on DSS-Induced Inflammatory Bowel Disease in Mouse Model System (DSS로 유도된 염증성 장 질환 마우스 동물모델에서 생식이 장관 임파조직내 면역조절 기능에 미치는 영향)

  • Lim, Beong-Ou;Jeong, Yong-Jun;Park, Mi-Hyoun;Kim, Jong-Dai;Hwang, Sung-Joo;Yu, Byung-Pal
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.1
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    • pp.32-42
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    • 2007
  • This study was conducted on the immunoregulatory effect of Saengshik on gut-associated lymphoid tissue with inflammatory bowel disease. Although the contents of IgA increased in mesenteric lymph node, IgE content was suppressed by Saengshik. The same results were found in spleen, but IgA and IgE responses were very weak. Concentration of fecal IgA was high from the first day through the third day in Saengshik group. In DSS + Saengshik group, concentration of IgA was high till the 2nd day and it maintained the highest level among the test groups on 5th day. Concentration of IFN-gamma and IL-2 was the highest in the Saengshik group, but the concentration of TNF-alpha was lower in DSS + Saengshik compared to DSS. The expressions of STAT1 in Saengshik group were high, while those of STAT6 were low According to these findings, Saengshik exhibited effectiveness via increasing the IgA production, suppressing the IgE production, followed by inhibiting the production of IL-4 and IL-10. Saengshik also strengthened the immune system and alleviated injury in DSS -induced inflammation.

Focus Group Interview on Opinion about the Design and Management of Toothbrushing Facilities (양치시설 설계 및 운영에 대한 관련 실무자의 견해: 초점집단면접 연구)

  • Lee, Hyo-Jin;Paik, Dai-Il
    • Journal of dental hygiene science
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    • v.16 no.2
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    • pp.118-126
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    • 2016
  • This study collected opinions about the design and management of toothbrushing facilities from schoolteachers and the people in charge of public centers, through a focus group interview (FGI), for the development of guidelines. Four moderators conducted eight FGIs of 32 subjects in charge of toothbrushing facilities across all the regions. The qualitative data was analyzed by constant comparative analysis. The findings indicated that more students utilized the facility and brushed their teeth after the installation of toothbrushing facilities. To enhance students' accessibility and safety, guidelines for design and management guidelinesfor toothbrushing facilities need to be developed; the location, sink height and depth, drain size, etc. should be comprehensively considered in the development of the guidelines. This study can help to improve students' satisfaction by enhancing the accessibility, safety, and functionality of toothbrushing facilities, and by providing fundamental data for toothbrushing facilities' development. Finally, this study can maximize the effects of management of toothbrushing facilities management on the improvement of students' oral health and behaviors.

A Study on Elution Behavior of Polystyrene Copolymers in Gel Permeation Chromatography (겔 투과 크로마토그래피에서 폴리스티렌 혼성중합체들의 용리거동에 관한 연구)

  • Lee Dai Woon;Eum Chul Hun
    • Journal of the Korean Chemical Society
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    • v.36 no.1
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    • pp.87-94
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    • 1992
  • The elution behavior of polystyrenes(PS), polymethylmethacrylates (PMMA), polybutadienes(PB), PS-PMMA(SM) block copolymers and PS-PB star shaped copolymers on the cross-linked polystyrene gels was studied. An interpretation was proposed for the plots of log hydrodynamic volume versus retention volume of solutes in the mobile phases such as tetrahydrofuran, toluene, chloroform, methylene chloride and tetrahydrofuran-cyclohexane mixture. In order to predict the retention of solutes from their physical properties, multiple stepwise regression analysis was applied to obtain the correlation. The distribution coefficients($K_p$) of solute-gel interactions in GPC for homopolymers and PS copolymers were also obtained in terms of network-limited separation mechanism. In the cases of PS and PB, $K_p$ values approach unity, while $K_p$ values for PMMA decrease as MW increase in the good solvent, but in poor solvent, $K_p$ values increase as MW increase. $K_p$ values of PS copolymers are dependent on their MW and composition, therefore, morohology of SM block copolymer is predicted to be random phase. A single universal plot of log[η]M vs. $(V_r-V_o)/K_p$

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Selective blockade of spinal D2DR by levo-corydalmine attenuates morphine tolerance via suppressing PI3K/Akt-MAPK signaling in a MOR-dependent manner

  • Dai, Wen-Ling;Liu, Xin-Tong;Bao, Yi-Ni;Yan, Bing;Jiang, Nan;Yu, Bo-Yang;Liu, Ji-Hua
    • Experimental and Molecular Medicine
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    • v.50 no.11
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    • pp.6.1-6.12
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    • 2018
  • Morphine tolerance remains a challenge in the management of chronic pain in the clinic. As shown in our previous study, the dopamine D2 receptor (D2DR) expressed in spinal cord neurons might be involved in morphine tolerance, but the underlying mechanisms remain to be elucidated. In the present study, selective spinal D2DR blockade attenuated morphine tolerance in mice by inhibiting phosphatidylinositol 3 kinase (PI3K)/serine-threonine kinase (Akt)-mitogen activated protein kinase (MAPK) signaling in a ${\mu}$ opioid receptor (MOR)-dependent manner. Levo-corydalmine (l-CDL), which exhibited micromolar affinity for D2DR in D2/CHO-K1 cell lines in this report and effectively alleviated bone cancer pain in our previous study, attenuated morphine tolerance in rats with chronic bone cancer pain at nonanalgesic doses. Furthermore, the intrathecal administration of l-CDL obviously attenuated morphine tolerance, and the effect was reversed by a D2DR agonist in mice. Spinal D2DR inhibition and l-CDL also inhibited tolerance induced by the MOR agonist DAMGO. l-CDL and a D2DR small interfering RNA (siRNA) decreased the increase in levels of phosphorylated Akt and MAPK in the spinal cord; these changes were abolished by a PI3K inhibitor. In addition, the activated Akt and MAPK proteins in mice exhibiting morphine tolerance were inhibited by a MOR antagonist. Intrathecal administration of a PI3K inhibitor also attenuated DAMGO-induced tolerance. Based on these results, l-CDL antagonized spinal D2DR to attenuate morphine tolerance by inhibiting PI3K/Akt-dependent MAPK phosphorylation through MOR. These findings provide insights into a more versatile treatment for morphine tolerance.

A Study on the Dynamic Correlations between Korean Housing Markets (국내 주택시장의 동태적 상관관계 분석)

  • Shin, Jong Hyup;Seo, Dai Gyo
    • Korea Real Estate Review
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    • v.24 no.1
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    • pp.15-26
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    • 2014
  • Using multivariate GARCH model, we estimate the relationship between the housing sale prices and lease prices in the Korean housing market. In the analysis of relationship between the rate of changes in sale and lease prices, the correlation coefficient of the apartment and detached house is higher than that of the townhouse. By housing type, the correlation coefficient between detached house and townhouse is higher than between apartment and detached house or apartment and townhouse. By housing size, there are no significant different results between the sales price and the rental price. The correlation coefficient between medium and small size is the highest in the apartment housing market, whereas the correlation coefficient between large and medium size is the highest in the detached housing market, resulting from the fact that people may be more interested in medium- and small-sized apartment and large- and medium-sized detached house. In the detached housing market, the correlation coefficient between large-medium size and medium-small size in the rental price is higher than that of sales price. This result implies that the process of the decision making between purchasing and leasing a house might be different.

The Effect of Perceived Organizational Supports, Perceived Supervisory Supports and Perceived Peer Supports on Innovative Behavior: Focused on the Job Embeddedness of Automotive Industry Research Institute Using VR Technology

  • Dai, Hao;Zhang, Hao
    • Journal of the Korea Society of Computer and Information
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    • v.24 no.10
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    • pp.139-147
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    • 2019
  • In this paper, we propose to explore ways to create internal motivation to enhance the job disposition of Korean and Chinese employees. In order to achieve this goal, we would like to select parameters that may trigger an increasing number of internal motivations for interest in recent years: perceived organisational support, perceived provider support, and perceived peer support, and to explore the impact on job embeddedness. I would like to find out the effect of job embodiedness on involved beauty. The process also seeks to verify that China and South Korea are different. To verify the research theory of this research, we will use a questionnaire to researchers of automotive companies using VR technology in Gyeonggi-do, Korea and Hadong-seong, China, and conducted a survey. A total of 200 pairs of questionnaires were distributed to the study subjects, 195 of which were retrieved, 191 of which eliminated unfaithful responses were used for the final analysis. The collected data were used for frequency analysis, exploratory factor analysis, reliability analysis, confirmatory factor analysis, correlation analysis, structural equation analysis. The results of this study, identified through empirical analysis, can be summarized as follows: First, Perceived Organizational Support, Perceived Supervisor Support will have a positive impact on Job Embeddedness. Second, Job Embeddedness will have a positive influence on the Innovative Beehivior. Third, Perceived Organizational Support, Perceived Supervisor Support will have a partial positive effect on Innovative Behavior. Fourth, the formation process and influence of Job Embeddedness of corporate employees in Korea and China are partly different, the report showed.

The Effect of Leader's Coaching and LMX on Innovative Behavior of Automotive VR Technology Researcher: Focusing on Mediated Effect of Resilience

  • Dai, Hao;Kim, Tae Hyun;Yang, Xiao Jing
    • Journal of the Korea Society of Computer and Information
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    • v.24 no.11
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    • pp.163-170
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    • 2019
  • In this study, we tried to demonstrate the influence of leader's coaching and LMX on the member's innovation vibrator and to provide implications. This study reviewed the reader's coaching, LMX, the theories of the member's innovation behabior, resiliency, and the studies and discussions that have been carried out in the past, and finally produced five hypotheses. To verify this research theory, this study collected data from 320 automotive company researchers using VR interaction technology. The collected data were used for frequency analysis, reliability analysis, exploratory factor analysis, confirmatory factor analysis, structural equation analysis. An Empirical Analysis are confirming a result of this study can be summarized as follows. First, leader's coaching have a positive effect on resilience. Second, is have a positive effect on resilience lmx. Third, the researchers is high resilience innovative behavior to promote positive influence. Fourth, resilience leader's coaching the innovative behavior the impact on. Fifth, are lmx resilience innovative behavior the impact on. Through the conclusion and discussion section, the theoretical practical significance of this study and the limitations of the research and the direction of future research are presented.