• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.3
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• pp.187-192
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• 2003

This study was conducted to investigate dry matter yield and quality of six turf grasses, and thus to apply its result into making mixed species in the mixtures. The six turf grasses were Kentucky bluegrass(Newport), red fescue(Salem), creeping bentgrass(Crenshaw), perennial ryegrass(Palmer Ⅱ), tall fescue(Rebell Jr.) and redtop(Barricuda). This experiment was carried out for 3-years at grassland experimental field of Chungnam National University. The dry matter(DM) yield obtained in tall fescue and redtop was higher than those of the other species(p<0.05). Consideration of crude protein content and dry matter digestibility(DMD), the species of high quality compared to the other species were creeping bentgrass, perennial ryegrass, and Kentucky bluegrass. The crude protein dry matter yield was higher in Kentucky bluegrass and redtop, it was lower in red fescue(p<0.05). On the other hand, digestible dry matter(DDM) yield was higher in tall fescue and redtop, but lower in creeping bentgrass and red fescue(p<0.05). Based on the result mentioned above, therefore, it is suggested that turf grasses such as tall fescue, Kentucky bluegrass and redtop are recommended as species of turf grass which could be used as bottom grasses in mixtures.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.1
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• pp.45-51
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• 2014

This study was conducted to investigate the feeding effects of total mixed fermentations (TMF) with spent mushroom (Flammuliua velutipes) and wet brewer's grain on estimating the productivity and nutrient utilization of Korean black goats. Four experimental groups were set up with each of the 5 animals for a 30-day feeding trial on growth performance. 12 black goats with regard to nutrient digestibility were allocated to 4 groups of 3 animals each by a latin-square design control group (offered concentrate and rice straw) and 3 treatment groups (TMF with 15% spent mushroom and 25% wet brewer's grain (T1), 10, 30% (T2) and 5, 35% (T3)). The average daily gain (ADG) of the control and T1 treatment groups was higher than that of the T3 treatment group (p<0.05). Dry matter (DM) and organic matter (OM) intake (kg/head) for T1 and T2 treatment groups were significantly higher than those of the control group (p<0.05). The digestibility of DM and OM for the control group (62.39~63.39%) and the T1 treatment group (65.02~65.25%) were significantly higher than those of the T3 treatment group (56.39, 59.01%) (p<0.05). Further, nitrogen retention was significantly higher in the T1 treatment group (p<0.05). Thus, these results conveyed that productivity and nutrient utilization were similar between TMF with spent mushroom and wet brewer's grain and commercial feeds. However, this study also suggested that feed must contain 25~30% spent mushroom due to the fact that the productivity of Korean black goats was decreased by increasing the content of spent mushroom.

• Journal of Life Science
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• v.29 no.10
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• pp.1152-1158
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• 2019

Rudbeckia laciniata var. hortensis Bailey is used in home remedy for colic and gastritis in South Korea. Although Rudbeckia laciniata var. hortensis Bailey is used extensively for home remedies, no single study on its efficacy exists. In this study, we investigated the anti-obesity effects of Rudbeckia laciniata var. hortensis Bailey. The anti-obesity effect of a 70% ethanol extract from Rudbeckia laciniata var. hortensis Bailey on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated with an Oil Red O assay, western blot analysis, and mRNA analysis. Compared to the control (only treated with DM), the 70% ethanol extract of Rudbeckia laciniata var. hortensis Bailey significantly inhibited adipocyte differentiation and intracellular triglyceride (TG) levels at a concentration of $100{\mu}g/ml$. To determine how the TG content was reduced, we measured the level of protein and mRNA expression of obesityrelated agents, such as peroxisome proliferators-activated receptor ${\gamma}$ ($PPAR{\gamma}$), CCAAT/enhancer- binding protein ${\alpha}$ ($C/EBP{\alpha}$), AMP-activated protein kinase (AMPK) phosphorylation, LPL, and FAS. As a result, the 70% ethanol extract of Rudbeckia laciniata var. hortensis Bailey significantly increased the expression of AMPK and decreased the expression of genes related to adipogenesis and fat storage, such as $PPAR{\gamma}$, $C/EBP{\alpha}$, LPL, and FAS.

• Animal Bioscience
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• v.34 no.2
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• pp.223-232
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• 2021

Objective: To investigate the improvement in utilization efficiency of total mixed ration (TMR) on Tibetan plateau, TMR were ensiled with different additives. Methods: A total of 150 experimental silos were prepared in a completely randomized design to evaluate the six treatments: i) control (without additive), ii) Lactobacillus buchneri (L. buchneri), iii) acetic acid, iv) propionic acid, v) 1,2-propanediol; and vi) 1-propanol. After 90 days of ensiling, silos were opened for fermentation quality and in vitro analysis, and then subjected to an aerobic stability test for 14 days. Results: Treating with L. buchneri, acetic acid, 1,2-propanediol and 1-propanol decreased propionic acid contents and yeast number, whereas increased (p<0.05) pH, acetic acid and ethanol contents in the fermented TMR. Despite increased dry matter (DM) loss in the TMRs treated with 1,2-propanediol and 1-pronanol, additives did not affect (p>0.05) all in vitro parameters including gas production at 24 h (GP24), GP rate constant, potential GP, in vitro DM digestibility and in vitro neutral detergent fibre digestibility. All additives improved the aerobic stability of ensiled TMR to different extents. Specially, aerobic stability of the ensiled TMR were substantially improved by L. buchneri, acetic acid, 1,2-propanediol, and 1-propanol, indicated by stable pH and lactic acid content during the aerobic stability test. Conclusion: L. buchneri, acetic acid, 1,2-propanediol, and 1-propanol had no adverse effect on in vitro digestibility, while ensiling TMR with the additives produced more acetic acid and ethanol, subsequently resulting in improvement of aerobic stability. There is a potential for some fermentation boosting additives to enhance aerobic stability of fermented TMR on Tibetan plateau.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.408-414
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• 2021

This study aimed to improve the production of bioactive materials with antioxidant activity using a fermented Luffa aegyripia extract and improve the anticancer effect by enhancing UV absorption and inhibiting melanoma cell growth. The total phenolic content (TPC) and antioxidant activity of the fermented extract were 30.23 mg GAE/g DM and 45.12%, respectively, which was 1.4 times higher than that of the hot-water extract (HWE). The fermented extract showed a UV adsorption rate of 53.9%, which was 1.5 times higher than HWE, and it was concluded that UV absorption was increased by TPC, which was increased through the fermentation of L. aegyptiaca extracts using Lactobacillus. In the anticancer effect test, fermented and HWE extracts had carcinogenic effects of 1.0 and 2.0 mg/mL, respectively. This suggests that the increased antioxidant activity due to the increase in TPC caused by fermentation contributed to the anticancer effect. The UV absorption rate of fermented extracts was 2.4 times higher than HWE, giving them potential use as cosmetics and pharmaceutical materials with high polyphenol contents and antioxidant properties and skin cancer prevention.

• Korean Journal of Organic Agriculture
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• v.29 no.4
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• pp.613-623
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• 2021

This study was conducted to investigate the effect of addition levels of coffee and green tea by products extract including polyphenols through hot water extraction on rumen fermentation. The treatment groups consisted of coffee extract (CO), green tea extract (GR) and mixed extract (MIX), and the addition level was 10 µL, 20 µL and 30 µL of three levels. The experiment consisted of a total of 10 experimental groups including the control group, and a full factorial design was used. The effect of polyphenol addition in coffee and green tea by-products was analyzed through main and interaction effect of statistical analysis. The total polyphenol content of the extracts was 106.15, 79.10 and 185.25 ㎍ GAE/g DM for coffee by-product, green tea by-product and mixture, respectively. Total gas production was significantly lower in the treatment groups than in the control (114.00 mL/gDM) (p<0.05). Methane emission tended to decrease as the polyphenol addition level increased. Moreover, the MIX showed the lowest methane emission when 30 µL was added (p<0.05). Volatile fatty acids showed a significant difference compared to the treatment group as a control (98.06 mM) (p<0.05), but there was no change according to the level of polyphenols. As a result of the main effect and interaction, it is thought that the effect on methane reduction and improvement of rumen fermentation in MIX20 can be expected. In a series of studies, the addition of 20 µL of a blended extract of coffee and green tea by-products is thought to reduce methane to levels that do not inhibit rumen fermentation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.307-312
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• 2009

This experiment was conducted to compare the agronomic characteristics and productivity of induced annual legumes at paddy field of Cheonan and Naju from 2004 to 2006. Chinese milk vetch (wild type), crimson clover (C.V. Linkarus), forage pea (C.V. Austrian pea), hairy vetch (C.V. Oregon commen) were used in this study. At the Naju region, the earliest flowering on 2nd may was found in Chinese milk vetch, while the latest on 18th May in forage pea. Fresh, dry matter (DM) and crude protein (CP) yields of crimson clover were highest as 28,870, 6,986 and 943 kg/ha, respectively. but the yield of chinese milk vetch was low by 9,885, 1,749 and 338 kg/ha. At the Cheonan region, the most fast flowering date variety was Chinese milk vetch, hairy vetch was very late as 19th May. Fresh, DM and CP yields of hairy vetch were highest as 7,916, 1,141 and 113 kg/ha, respectively. but the yield of chinese milk vetch showed the lowest by 270, 42 and 8 kg/ha. The CP content of annual legumes was 14.3%. chinese milk vetch showed the highest CP content by 19.3% and hairy vetch showed the lowest content by 9.9%. The present data showed that the productivity was closely dependent on winter hardness, in conclusion, crimson clover at Naju and hairy vetch at Cheonan region was suggested to be the most proper species as winter crop after rice harvest on paddy field when considered the winter hardiness.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.2
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• pp.98-104
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• 2010

Excess nitrogen (N) uptake of rice, which could cause much lodging, disease and reduction of rice quality, could be occurred at the paddy field with previous upland condition at which much soil N could be mineralized by soil-drying effect. N fertilizers of 0, 3, and 6 kg N $10a^{-1}$ were applied to early-maturity rice, cultivar Joanbyeo at the paddy field of first and second year after upland condition, and rice growth and nitrogen uptake were investigated to know the increase of rice N uptake at the paddy field with previous upland condition for one-year. Total dry matter (DM) and N uptake of rice at the paddy field with previous upland condition increased more than continuous paddy field. Total DM and N uptake of rice at the paddy field with previous upland condition increased linearly to N fertilizer 6 kg $10a^{-1}$ at the paddy field owing to vigorous growth compared to continuous paddy field. Rice N uptake was higher at the paddy field of the first year than the second year after upland condition in considering N uptake at the plot of no N fertilizer. Vigorous growth at the paddy field with previous upland condition resulted in higher rice yield which was related with high panicle and spikelet, but much N fertilizer as much as 6 kg $10a^{-1}$ at the paddy field with previous upland condition resulted in higher lodging and protein content of brown and milled rice. Particularly, protein content of brown and milled rice increased more when the same N fertilizer was applied two times splitly at transplanting and panicle initiation stage than when N fertilizer was applied one time at transplanting as basal N. N application with under 3 kg $10a^{-1}$ as only basal N was recommended at the paddy field with previous upland condition to obtain high quality rice without lodging.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.2
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• pp.135-141
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• 2016

This study amied to determine the effect of species and seed mixture on productivity, botanical composition and forage quality in middle mountainous region (Geosan) pasture. Total seven experimental pastures (T1 : Orchardgrass, T2 : Timothy, T3 : Tall fescue, T4 : Perennial ryegrass, T5 : Kentucky bluegrass, T6 : Timothy + Orchardgrass + Tall fescue + Alfalfa, T7 : Timothy + Orchardgrass + Kentucky bluegrass + White clover) were established in autumn, 2014 and evaluated for productivity and agronomic characteristics for 2015. Plant heigh was high in Orchardgrass and Perennial ryegrass pasture. Dry matter (DM) content was high in Kentucky bluegrass. Botanical composition in $1^{st}$ harvest was high in shrub, but the grass composition was high in $2^{nd}$ and $3^{rd}$ harvest. Fresh and DM yield were higher in Orchardgrass and Perennial ryegrass (p<0.05); whreras, Kentucky bluegrass was the lowest. In seed mixture pasture, yields of T6 plot (Tall fescue) were higher than those of T7 (Kentucky bluegrass). Average CP (crude protein) content of $2^{nd}$ and $3^{rd}$ cutting time was 16.91 and 14.79%, respectively and Kentucky blue grass was the highest in $3^{rd}$ cutting time by 17.57%. IVDMD (in vitro dry matter digestibility) showed low in $1^{st}$ cutting and Kentucky bluegrass was the lowest in every cutting time. TDN (total digestible nutrient) content was the highest in $3^{rd}$ cutting and the lowest in $1^{st}$ cutting. These results indicated that Orchardgrass, Perennial ryegrass and T6 are recommendable for productivity and Timothy, Perennial ryegrass and T7 are recommendable for forage quality.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.266-272
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• 2018

In this study, we created grassland establishment(paddock No.39), grassland improvement(Paddock No.44), and tall fescue seeding(paddock No.64), and the rate botanical composition, DM yield, nutritive value were examined through 2016 to 2017. The rate of botanical composition was 81% in 2016, 75% in 2017, up to 21% higher than No.39 67%, 60%, No.44 58% and 54%. The annual average DM yield was the highest at 13,234kg/ha in the district, followed by No.39, No.44, followed by 10,636kg/ha and 10,235kg/ha, respectively. The crude protein content was the highest at No.39 12.16%, with No.44 and No.64 showing 10.7%, respectively. NDF content (average for two years) was 55.90% in No.44 and No.39, slightly lower than 58.42% and 57.00%, respectively. The two-year ADF average content was 31.07% for the same trend as the NDF capacity in the No.44, below 31.71% and 32.65%, respectively. To sum up the results, Although there was a high level of incentive sowing plot(No.39) in nutritive value, considering the botanical composition and productivity of the pasture, it is also deemed desirable to have a tall fescue plot(No.64).