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http://dx.doi.org/10.5352/JLS.2019.29.10.1152

Inhibitory Effect of the Ethanol Extract of Rudbeckia laciniata var. hortensis Bailey on Adipocyte Differentiation in 3T3-L1 Cells  

Nam, Gun He (Department of Biological Science and Biotechnology, Hannam University)
Wee, Ji-Hyang (Department of Food Science & Bio Technology, Shinansan University)
Kim, Sang Yung (Department of Food Science & Bio Technology, Shinansan University)
Baek, Ji-Young (Department of Pharma-Gene Co., Ltd.)
Kim, Young Min (Department of Biological Science and Biotechnology, Hannam University)
Publication Information
Journal of Life Science / v.29, no.10, 2019 , pp. 1152-1158 More about this Journal
Abstract
Rudbeckia laciniata var. hortensis Bailey is used in home remedy for colic and gastritis in South Korea. Although Rudbeckia laciniata var. hortensis Bailey is used extensively for home remedies, no single study on its efficacy exists. In this study, we investigated the anti-obesity effects of Rudbeckia laciniata var. hortensis Bailey. The anti-obesity effect of a 70% ethanol extract from Rudbeckia laciniata var. hortensis Bailey on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated with an Oil Red O assay, western blot analysis, and mRNA analysis. Compared to the control (only treated with DM), the 70% ethanol extract of Rudbeckia laciniata var. hortensis Bailey significantly inhibited adipocyte differentiation and intracellular triglyceride (TG) levels at a concentration of $100{\mu}g/ml$. To determine how the TG content was reduced, we measured the level of protein and mRNA expression of obesityrelated agents, such as peroxisome proliferators-activated receptor ${\gamma}$ ($PPAR{\gamma}$), CCAAT/enhancer- binding protein ${\alpha}$ ($C/EBP{\alpha}$), AMP-activated protein kinase (AMPK) phosphorylation, LPL, and FAS. As a result, the 70% ethanol extract of Rudbeckia laciniata var. hortensis Bailey significantly increased the expression of AMPK and decreased the expression of genes related to adipogenesis and fat storage, such as $PPAR{\gamma}$, $C/EBP{\alpha}$, LPL, and FAS.
Keywords
Adipocytes; $C/EBP{\alpha}$; FAS; $PPAR{\gamma}$; Rudbeckia laciniata var. hortensis Bailey;
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