• Journal of Oral Medicine and Pain
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• 제20권2호
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• pp.497-513
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• 1995

Human genomic Deoxyribonucleic acid(DNA) was extracted from teeth by boiling, salting-out, phenol, boiling-phenol methods. The author compared DNA concentration and its purity, the accuracy of sex determination and the results of the D1S80 locus detection among above 4 methods. The following results were obtained : 1. DNA concentration was the highest in pulp with salting-out method and DNA purity was higher in pulp with salting-out and phenol methods than other 2 methods. 2. Sex determination was possible using of the pulp and the dentin of the teeth with four methods but, it was impossible in the enamel and some pulp with boiling method. 3. Amplification of D1S80 locus occurred from pulp and dentin with salting-out, phenol, and boiling-phenol methods. 4. There are no differences among the amplification of X-Y homologus amelogenin gene by application of 4 methods and salting-out, phenol methods efficiently makes available to amplification of D1S80 locus. From the investigation DNA extraction, sex determination, amplification of D1S80 locus was successfully accomplished with salting-out, phenol, boiling-phenol methods Therefore above 3 methods are available and applicable as forensic odontology for individual identification.

• 대한전자공학회논문지SD
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• 제47권6호
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• pp.35-42
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• 2010

본 논문에서는 무선 통신 응용 시스템에 적합 하도록 10비트 80MHz 전류구동 방식의 D/A 변환기를 제안하였다. 제안한 회로는 $0.18{\mu}m$ CMOS n-well 1-poly 6-metal 공정을 이용하여 구현하였다. 10비트 중에서 LSB 4비트는 이진 디코더를 사용 하였으며, ULSB 3비트와 MSB 3비트는 온도계 디코더를 사용한 혼합구조를 채택하였다. 구현된 D/A 변환기의 측정결과, 샘플링 주파수가 80MHz, 입력 주파수 1MHz에서 SFDR은 60.42 dBc, 유효비트수는 8.75 비트를 보여주었다. INL/DNL은 ${\pm}$0.38LSB/${\pm}$0.32LSB로 측정되었으며, 글리치 에너지는 4.6 $pV{\cdot}s$로 나타났다. 전력 소모는 1.8V 전원전압에서 최대 속도인 80MHz일 때 48mW로 측정되었다.

• 한국수산과학회지
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• 제48권1호
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• pp.76-81
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• 2015

The effects of temperature, photon irradiance, and photoperiod on the growth of Sargassum horneri embryos were examined for female plants collected at Chokpo in Tongyeoung, Gyeongnam Prefecture, Korea on 27 December 2011. Mature plant receptacles were detached, and fertilized eggs were cultured in the laboratory at temperatures of $5-25^{\circ}C$ with photon irradiances of $10-80{\mu}mol\;m^{-2}s^{-1}$ under 14L:10D and 10L:14D photoperiods. Germination and embryo growth were rapid at $20-25^{\circ}C$ and $40{\mu}mol\;m^{-2}s^{-1}$ under 14L:10D. The number of rhizoids in the germinated young thalli was high at high photon irradiances ($40-80{\mu}mol\;m^{-2}s^{-1}$) and $15-20^{\circ}C$. At $20^{\circ}C$ and 14L:10D, vigorous growth of main and lateral branches was observed; however, at $5^{\circ}C$ under both photoperiods, plant growth decreased markedly. In the present study, S. horneri grew well at high temperatures ($20-25^{\circ}C$) and high photon irradiances ($40-80{\mu}mol\;m^{-2}s^{-1}$) with a 14L:10D photoperiod.

• 대한전자공학회논문지SD
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• 제45권4호
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• pp.13-20
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• 2008

본 논문에서는 무선 통신 응용 시스템에 적합하도록 슈도-세그멘테이션 기법을 이용하여 저 전력 12비트 80MHz D/A 변환기를 CMOS 0.18um n-well 1-Poly/6-Metal 공정으로 설계하였다. 슈도-세그멘테이션 기법은 간단한 병렬 버퍼로 구성된 이진 디코더를 사용함으로써 구조적으로 간단해지며 저 전력으로 구현이 가능하다. 또한, 스위칭 코어 회로에 글리치 억제 회로와 입력신호의 스윙을 감소시키는 구동 회로를 설계함으로써 추가적인 스위칭 잡음을 줄일 수 있었다. 측정 결과 제안한 저 전력 12bit 80MHz CMOS D/A 변환기는 샘플링 주파수 80MHz일 때, 입력 주파수 1MHz에서 SFDR은 66.01dBc, 유효비트수는 10.67비트를 보여주었다. INL/DNL은 ${\pm}1.6LSB/{\pm}1.2LSB$로 측정되었으며, 글리치 에너지는 $49pV{\cdot}s$로 나타났다. 전력 소모는 1.8V 전원 전압에서 최대 속도인 80MHz일 때 46.8mW로 측정되었다.

• 한국건설순환자원학회논문집
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• 제7권4호
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• pp.375-382
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• 2019

본 연구에서는 개발된 프린팅 재료 및 장비를 사용하여 대상 구조물의 시공성을 파악하기 위하여 기초 성능평가 프린팅 실험을 수행하였다. 3D 프린팅 콘크리트 구조물의 시공성은 콘크리트 필라멘트 재료 특성, 시공 프로세스 및 구조물의 기하특성에 영향을 받는다. 따라서, 콘크리트 구조물의 적층하는 동안의 시공성을 파악하기 위한 실험변수로서 노즐 이동속도(=80 및 100mm/s), 토출 버킷의 스크류 분당회전속도 (RPM) 및 구조물 벽체 길이의 변장비(1.67 및 5.00)를 고려하였다. 실험변수에 따른 3D 콘크리트 구조물의 최대 적층 수 및 파괴 패턴을 토대로 시공성을 분석하였다. 본 연구에서, 80mm/s의 노즐 이동속도 및 1.67의 변장비가 3D 프린팅 시공성에 유리함을 나타낸다. 또한, 적층 시 구조물 하단부의 상대변위측정을 통해 구조물의 전도 파괴 과정을 분석하였다.

• 한국잡초학회지
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• 제16권1호
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• pp.36-41
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• 1996

본(本) 실험(實驗)은 논 난방제(難防除) 잡초(雜草)로 지적(指摘)되고 있는 벗풀에 대(對)한 2,4-D, 2,4-D ethylester 및 bentazone의 살초효과(殺草效果)와 효과적(效果的)인 사용법(使用法)을 밝히고자 수행(遂行)되었다. 1. 2,4-D와 2,4-D ethylester는 80일(日)까지도 벗풀에 대(對)하여 100%의 살초효과(殺草效果)를 나타내었으나, bentazone은 60일(日) 이후(以後) 처리(處理)에서 살초효과(殺草效果)가 감소(減少)되었다. 2. Bentazone은 생장점(生長點)으로 이행(移行)이 어려운 반면(反面), 2,4-D와 2,4-D ethylester는 생육후기(生育後期)임에도 지하부(地下部)의 생장점(生長點)으로 쉽게 이행(移行)되어 생장점(生長點)을 파양(破壤)하였다. 3. 2,4-D와 2,4-D ethylester는 3cm 담수구(湛水區)에서도 80일(日)까지 완전방제(完全防除)가 가능(可能)한 반면(反面), bentazone은 1cm 이상(以上) 담수구(湛水區)에서 효과(效果)가 현저(顯著)히 감소(減少)되었다. 4 괴경현성(塊莖形成)에 있어서 2,4-D와 2,4-D ethylester의 한계(限界) 처리수준(處理水準) 및 시기(時期)는 각각(各各) 기준량(基準量)의 1/4량(量)인 7g ai/10a과 1/8량(量)인 6g ai/10a 60일(日)이었다.

• Journal of Oral Medicine and Pain
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• 제20권2호
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• pp.515-528
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• 1995

The human genomic deoxyribonucleic acid(DNA) was extracted from the pulp, dentin of 22 teeth by clelex, phenol methods. Samples of the tooth-derived DNA were amplified by polymerase chain reaction(PCR), electrophosed for sex determination by detection of X-Y homologus amelogenin gene and D1S80 locus detection The following results have been achieved. 1. Chelex and phenol method are effective to sex determination in the pulp and dentin 2. Chelex method is not suitable for detection of D1S80 locus. 3. Concentration and purity of DNA for teeth using chelex method is lower than using phenol method. From the above investigation, chelex method is simple, rapid for sex determination, but it is not suitable for detection of VNTRs.

• BMB Reports
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• 제28권4호
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• pp.359-362
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• 1995

The highly polymorphic variable number of tandem repeat (VNTR) loci in the human genome are informative markers for the genetic characterization of individuals in the paternity test and forensic science as well as for the study of human disease. In this study, VNTR loci D1S80 and D2S123 have been amplified by PCR and the amplified length polymorphic alleles were detected with a discontinuous vertical PAGE system and silver staining. For explicit DNA typing, PCR optimization, in which amplification efficiencies are similar over a wide range of allele sizes, non-specific amplifications are minimal, and new longer alleles have high amplification efficiency, has been performed by changing the PCR reaction buffer composition and thermal cycling conditions. It turned out that adding an appropriate amount of Tween 20 and NP40 to the PCR reaction buffer and raising the annealing temperature to $68^{\circ}C$ in thermal cycling made it possible for optimal VNTR loci amplification. A modified PAGE system for VNTR separation was established. Under these conditions, new longer alleles in the 01580 locus were discovered and 025123 pattern changes in colorectal tumors were observed. These technical tips are valuable for detecting various amplified fragment length polymorphisms.

• KSBB Journal
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• 제22권1호
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• pp.58-61
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• 2007

본 연구에서는 은행잎에 존재하는 생리활성 물질이 추출용매와 추출조건에 따른 D. pteronyssinus에 대한 살충 실험을 통해 최적 추출 조건을 확립하였다. 은행잎 성분 중 bilobalide가 D. pteronyssinus에 살충효과가 있음을 검증하였으며, 다양한 은행잎 추출조건에 따른 살충실험을 한 결과 48시간 이후 약 68%$\sim$80%의 치사율을 보였다. 종합적으로 판단해 볼 때 경제성과 치사율을 고려하면 80$^{\circ}C$에서 온수추출물 (GLW80)이 가장 최적추출 조건이다.

• 핵의학기술
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• 제22권1호
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• pp.80-83
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• 2018

Purpose Assessment of Serum Thyroglobulin (sTg) value in total thyroidectomy patients having an ablation dose of radioactive iodine indicates remaining cancer or metastasis. Especially, sTg in patients on withdrawal thyroxine or thyrogen administration for radioiodine ablation is an important indicator to determine the direction of further treatment and prognosis. Current guidelines suggest measurement of sTg is performed at 72 hours after the last injection of thyrogen. and assumes that sTg reaches maximum serum levels at that time. The purpose of this study is to evaluate the variation of sTg measured after thyrogen administration. Materials and Methods We compared with sTg performed at 24hours(D0) and 72hours(D2) after the last injection of thyrogen. We reviewed D0 and D2 from 276 patients were divided them into three groups according to ablation dose of radioactive iodine, 5mCi(A group), 30~80mCi(B group) and 100~200mCi(C group). We used T-test for comparison between D0 and D2. sTg was measured in serum using immunoradiometric assay (Tg-plus RIA; BRAHMS, Berlin, Germany). Results There is no critical variation between D0 and D2 in A group(n=100)(P=0.32), The case of increase(D2>D0) is 45, no change(D2=D0) is 23, decrease(D2D0 is 91, D2=D0 is 28, D2D0 is 19, D2=D0 is 2. The biggest increase is 143.6 ng/mL from 98.4 to 242. Conclusion There was a significant difference in the group over 30mCi. and the case of D2>D0 is 45%, 58.7%, 90.5% for each group. therefore, D2 increased as the dose of radioactive iodine increased. Furthermore, the most sTg values of D0 and D2 are variation under 2.0 ng/mL, so reproducibility as well as sensitivity of sTg will be important at values below 2ng/mL.