• Title/Summary/Keyword: D10S

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Genetic Variants of Serum Proteins and Enzymes in Holstein-Friesian Cattle (홀스타인종 유우의 혈청단백질 및 효소의 유전적 변이체)

  • Sang, Byung Chan;Ryoo, Seung Heui;Seo, Kil Woong;Lee, Chang Soo
    • Korean Journal of Agricultural Science
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    • v.22 no.2
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    • pp.163-169
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    • 1995
  • This study was carried out to examine the genetic constitution of serum proteins and enzymes in Holstein Friesian cattle population. The genetic variants of post-transferrin-2(pTf-2), transferrin(Tf), post-albumin(pAlb), ceruloplasmin(Cp) and amylase-I(Am-I) were analyzed by using PAGE(polyacrylamide gel electrophoresis) and STAGE(starch gel electrophoresis). In serum proteins, the pTf-2 locus were observed to be controlled by codominant alleles designated F and S, and the distribution of genotypes were 76.34, 14.50 and 9.10% for pTf-2 FF, FS and SS types, respectively. The gene frequencies of the pTf-2 F and S allele were 0.836 and 0.164. The Tf locus were found to be controlled by four alleles, Tf A, D1, D2 and E at a single locus, and the distribution of genotypes were 6.11, 32.06, 19.08, 1.53, 10.69, 18.32, 9.92 and 2.29% for Tf AA, AD1, AD2, AE, D1D1, D1D2, D2D2 and D2E type, respectively. The gene frequencies of the Tf A, D1, D2 and E wee 0.321, 0.359, 0.298 and 0.019. The pAlb locus were identified to be genetically controlled by two alleles, pAlb F and S allele, and the distribution of genotypes were 32.06, 29.77 and 38.17% for pAlb FF, FS and SS types, respectively. The gene frequencies of the pAlb F and S allele were 0.461 and 0.531. The Alb locus were observed to be controlled by Alb A and B allele, and the gene frequencies of these were 0.996 and 0.004. In serum enzymes, the Cp locus were found to be controlled by F and S allele, and the distribution of genotypes were 46.57, 27.48 and 25.95% for Cp FF, FS and SS types, respectively. The gene frequencies of F and S allele were 0.603 and 0.394. The Am-I locus were observed to be controlled by Am-I B and C allele, and the distribution of genotypes were 39.69, 21.73 and 38.93% for Am-I BB, BC and CC types, the gene frequencies of Am-I B and C were 0.503 and 0.497, respectively.

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Impact Properties of S-2 Glass Fiber Composites with Multi-axial Structure (다축 구조 S-2 유리섬유 복합재의 충격 특성)

  • Song, S.W.;Lee, C.H.;Byun, J.H.;Hwang, B.S.;Um, M.K.;Lee, S.K.
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2005.04a
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    • pp.71-75
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    • 2005
  • For the damage tolerance improvement of conventional laminated composites, stitching process have been utilized for providing through-thickness reinforcements. 2D preforms were stacked with S-2 glass plain weave and S-2 glass MWK (Multi-axial Warp Knit) L type. 3D preforms were fabricated using the stitching process. All composite samples were fabricated by RTM (Resin Transfer Molding) process. To examine the damage resistance performance the low speed drop weight impact test has been carried out. For the assessment of damage after the impact loading, specimens were examined by scanning image. CAI (Compressive After Impact) tests were also conducted to evaluate residual compressive strength. Compared with 2D composites, the damage area of 3D composites was reduced by 20-30% and the CAI strength showed 5-10% improvement.

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Effects of Auxin, GA and Cytokinin on the Protein Synthesis (Accumulation) of Soybean (Auxin, GA 및 Cytokinin이 대두의 단백질합성 (축적)에 미치는 영향)

  • Yoo, Ki-Jung;Park, Chang-Kyu;Kim, Su-Il
    • Applied Biological Chemistry
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    • v.29 no.1
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    • pp.73-77
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    • 1986
  • Aqueous solutions of 2,4-D, BA or $GA_3(10^{-6},\;10^{-5},\;and\;10^{-4}M,\;respectively)$ were sprayed onto soybean (Glycine max) plants in the flowering stage, and proteins of immature (33days after flowering) and mature (77days after flowering) seeds were analyzed by electrophoresis to elucidate the effects of the growth reguators on protein synthesis or protein accumulation in the seeds. Accumulations of some proteins were altered by 2,4-D or BA at certain concentrations, but no proteins were affected by $GA_3$. The ${\alpha}\;and\;{\alpha}'$ subunits of 7S and acidic subunit of 11S disappeared in mature seeds after treatments at the flowering stage with 2,4-D or BA. The presence of ${\alpha}\;and\;{\alpha}'$ subunits of 7S and acidic subunit of 11S in immature seeds indicated that the absence of the above polypeptides in mature seeds did not result from inhibitions in syntheses of the polypeptides by the growth regulators. Disappearance of the above proteins in mature seeds seemed to be concerned with the action of specific proteolytic enzyme (s) (metalloendopeptidase?), and 2,4-D and BA might promote gene expression or activation of the enzyme.

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Assessment of Salt Damage for Upland-Crops in Dae-Ho Reclaimed Soil (대호 간척지 토양의 염농도별 밭작물의 염해 평가)

  • Lee, Seung-Heon;Yoo, Sun-Ho;Seol, Su-Il;An, Yeoul;Jung, Yeong-Sang;Lee, Sang-Mo
    • Korean Journal of Environmental Agriculture
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    • v.19 no.4
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    • pp.358-363
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    • 2000
  • This study was carried out to obtain the basic data for selecting the applicable crops in reclaimed land during desalinization period. A pot experiment was conducted with 5 different electrical conductivities of the saturated extracts $(ECe\;1,\;3,\;9,\;14,\;and\;16\;dS{\cdot}m^{-1})$ of soils taken from the Dae-Ho reclaimed tidal lands. Eight crops (Chinese cabbage, radish, tomato, red pepper, buckwheat, soybean, sesame, and green perilla) were grown for 37days. Plant height and number of leaves were surveyed on 2 and 4 weeks after seeding, and on harvest time (5 weeks). After harvest, dry weights of harvested crops were measured and soil chemical properties were analyzed. Emergence rates of crops were comparatively high except sesame. For sesame, there was no emergence at ECe over $3\;dS{\cdot}m^{-1}$. Growth and dry weight decreased significantly as increasing ECe. The ECe which decreased 50% of dry weight index were $14.2\;dS{\cdot}m^{-1}$ for radish, $11.4\;dS{\cdot}m^{-1}$ for Chinese cabbage, $10.2\;dS{\cdot}m^{-1}$ for red pepper, $8.9\;dS{\cdot}m^{-1}$ for buckwheat and green perilla, $8.6\;dS{\cdot}m^{-1}$ for soybean, and $8.9\;dS{\cdot}m^{-1}$ for tomato. At higher ECe that start the growth inhibition, increasing $1\;dS{\cdot}m^{-1}$ in ECe, 7.7, 6.5, 5.9, 5.6, 5.2, and 4.9% of dry weight decreased for buckwheat, green perilla, Chinese cabbage, radish, soybean, and tomato (red pepper), respectively. The critical value of ECe for crop survival except sesame was $15.4\;{\sim}\;23.1\;dS{\cdot}m^{-1}$.

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Electrostatic Immobilization of D-Xylose Isomerase to a Cation Exchanger for the Conversion of D-Xylose to D-Xylulose (D-xylose에서 D-xylulose로의 전환을 위한 D-xylose Isomerase의 정전기적 고정화)

  • Hang, Nguyen Thi;Kim, Sung-Gun;Kweon, Dae-Hyuk
    • Microbiology and Biotechnology Letters
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    • v.40 no.2
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    • pp.163-167
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    • 2012
  • Since D-xylose is not fermentable in Saccharomyces cerevisiae, its conversion to D-xylulose is required for its application in biotechnological industries using S. cerevisiae. In order to convert D-xylose to D-xylulose by way of an enzyme immobilized system, D-xylose isomerase (XI) of Escherichia coli was fused with 10-arginine tag (R10) at its C-terminus for the simple purification and immobilization process using a cation exchanger. The fusion protein XIR10 was overexpressed in recombinant E. coli and purified to a high purity by a single step of cation exchange chromatography. The purified XIR10 was immobilized to a cation exchanger via the electrostatic interaction with the C-terminal 10-arginine tag. Both the free and immobilized XIR10 exhibited similar XI activities at various pH values and temperatures, indicating that the immobilization to the cation exchanger has a small effect on the enzymatic function of XIR10. Under optimized conditions for the immobilized XIR10, D-xylose was isomerized to D-xylulose with a conversion yield of 25%. Therefore, the results of this study clearly demonstrate that the electrostatic immobilization of XIR10 via the interaction between the 10-arginine tag and a cation exchanger is an applicable form of the conversion of D-xylose to D-xylulose.

All-optical wavelength conversion of 2.5 Gb/s optical signals by four-wave mixing in a semiconductor optical amplifier (반도체 광 증폭기내에서의 4광파 혼합을 이용한 2.5Gb/s 광신호의 전광 파장변환)

  • 방준학;서완석;이성은
    • Journal of the Korean Institute of Telematics and Electronics D
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    • v.35D no.8
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    • pp.69-75
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    • 1998
  • We demonstrate wavelength conversion of 2.5Gb/s optical signals by four-wave mixing (FWM) in a semiconductor optical amplifier (SOA). We investigate the effect of input pump and signal powers on the coversion efficiency, optical signal-to-noise ratio (OSNR) and extinction ratio to be a measure of performance in a wavelength converter. As a result, we show that the maximum bit error rate (BER) performance can be obtained by co promising among high-vonversion efficiency (minimum Pprobe), high-OSNR (maximum Pprobe) and low-cross-gain saturation effects (Pprobe kept at least 6dB weaker than Ppump). In our experiment, we obtain optimum performance at +3 dBm pump power and -6dBm signal power. The power penalty incurred in the wavelength conversion can be minimized by careful selection of the input pump and signal powers. We show that about 0.5dB power penalty for 3.2nm wavelength coversion at 10-10 BER is achievable.

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A 2.5 V 10b 120 MSample/s CMOS Pipelined ADC with High SFDR (높은 SFDR을 갖는 2.5 V 10b 120 MSample/s CMOS 파이프라인 A/D 변환기)

  • Park, Jong-Bum;Yoo, Sang-Min;Yang, Hee-Suk;Jee, Yong;Lee, Seung-Hoon
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.39 no.4
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    • pp.16-24
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    • 2002
  • This work describes a 10b 120 MSample/s CMOS pipelined A/D converter(ADC) based on a merged-capacitor switching(MCS) technique for high signal processing speed and high resolution. The proposed ADC adopts a typical multi-step pipelined architecture to optimize sampling rate, resolution, and chip area, and employs a MCS technique which improves sampling rate and resolution reducing the number of unit capacitor used in the multiplying digital-to-analog converter (MDAC). The proposed ADC is designed and implemented in a 0.25 um double-poly five-metal n-well CMOS technology. The measured differential and integral nonlinearities are within ${\pm}$0.40 LSB and ${\pm}$0.48 LSB, respectively. The prototype silicon exhibits the signal-to-noise-and-distortion ratio(SNDR) of 58 dB and 53 dB at 100 MSample/s and 120 MSample/s, respectively. The ADC maintains SNDR over 54 dB and the spurious-free dynamic range(SFDR) over 68 dB for input frequencies up to the Nyquist frequency at 100 MSample/s. The active chip area is 3.6 $mm^2$(= 1.8 mm ${\times}$ 2.0 mm) and the chip consumes 208 mW at 120 MSample/s.

Inducing the Regeneration and Maturation of Free-living Gametophytes of Ecklonia stolonifera Okamura (Laminariales, Phaeophyta) (대형갈조류 곰피 (Ecklonia stolonifera Okamura) 유리배우체의 재생 및 성숙 유도)

  • Hwang, Eun-Kyoung;Gong, Yong-Gun;Ha, Dong-Soo;Park, Chan-Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.3
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    • pp.231-238
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    • 2010
  • The Induction of regeneration and maturation in the free-living gametophytes of Ecklonia stolonifera Okamura was studied at four temperatures (5, 10, 15, and $20^{\circ}C$), four levels of irradiance (5, 10, 20, and 40 ${\mu}mol\;m^{-2}s^{-1}$) and three photoperiods (14:10, 12:12, and 10:14 h L:D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under $5{\sim}10^{\circ}C$, 10 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D), whereas the conditions for male gametophytes were slightly different at $20^{\circ}C$, 40 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D). The sexual maturation of female and male gametophytes was facilitated at $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}s^{-1}$, 14:10 h (L:D). These results provide basic information for controlling the regeneration and maturation of the free-living gametophytes for artificial seed production of E. stolonifera.

Mycological Characteristics and Carbon Source Activities of Unrecorded Wild Yeast Species from Vineyard Soil in Korea (국내 포도밭 토양에서 분리된 야생 효모 미기록종들의 균학적 특성과 탄소원 활성)

  • Bong Soon Yim;Myung Kyum Kim
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.373-381
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    • 2023
  • This study aimed to isolate and characterize wild yeast strains from the vineyard soil in Korea. Twenty yeast strains were isolated from vineyard soil in Gimpo-si, Gyeonggi-do, Korea, where Vitis labrusca cv. Campbell Early is grown. Eighteen strains were previously recorded in Korea. The remaining two, Cyberlindnera mrakii VG-21-10C and Starmerella bacillaris GR9 were not previously recorded in Korea. The mycological characteristics of VG-21-10C and GR9 were investigated. Both were oval-shaped, convex, and smooth. However, differences were evident in colony color and carbon assimilation activities. Strain VG-21-10C is white-colored and assimilates glucose, glycerol, D-xylose, D-cellobiose, D-saccharose, and D-raffinose as carbon sources. Strain GR9 is cream-colored and assimilates glucose, D-saccharose, and D-raffinose as carbon sources.