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http://dx.doi.org/10.5657/kfas.2010.43.3.231

Inducing the Regeneration and Maturation of Free-living Gametophytes of Ecklonia stolonifera Okamura (Laminariales, Phaeophyta)  

Hwang, Eun-Kyoung (Seaweed Research Center, NFRDI)
Gong, Yong-Gun (Seaweed Research Center, NFRDI)
Ha, Dong-Soo (Seaweed Research Center, NFRDI)
Park, Chan-Sun (Department of Marine and Fisheries Resources, Mokpo National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.43, no.3, 2010 , pp. 231-238 More about this Journal
Abstract
The Induction of regeneration and maturation in the free-living gametophytes of Ecklonia stolonifera Okamura was studied at four temperatures (5, 10, 15, and $20^{\circ}C$), four levels of irradiance (5, 10, 20, and 40 ${\mu}mol\;m^{-2}s^{-1}$) and three photoperiods (14:10, 12:12, and 10:14 h L:D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under $5{\sim}10^{\circ}C$, 10 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D), whereas the conditions for male gametophytes were slightly different at $20^{\circ}C$, 40 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D). The sexual maturation of female and male gametophytes was facilitated at $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}s^{-1}$, 14:10 h (L:D). These results provide basic information for controlling the regeneration and maturation of the free-living gametophytes for artificial seed production of E. stolonifera.
Keywords
Ecklonia stolonifera; Free-living gametophyte; Maturation; Regeneration;
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