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Inducing the Regeneration and Maturation of Free-living Gametophytes of Ecklonia stolonifera Okamura (Laminariales, Phaeophyta)

대형갈조류 곰피 (Ecklonia stolonifera Okamura) 유리배우체의 재생 및 성숙 유도

  • 황은경 (국립수산과학원 해조류바이오연구센터) ;
  • 공용근 (국립수산과학원 해조류바이오연구센터) ;
  • 하동수 (국립수산과학원 해조류바이오연구센터) ;
  • 박찬선 (목포대학교 해양수산자원학과)
  • Received : 2010.03.18
  • Accepted : 2010.06.15
  • Published : 2010.06.30

Abstract

The Induction of regeneration and maturation in the free-living gametophytes of Ecklonia stolonifera Okamura was studied at four temperatures (5, 10, 15, and $20^{\circ}C$), four levels of irradiance (5, 10, 20, and 40 ${\mu}mol\;m^{-2}s^{-1}$) and three photoperiods (14:10, 12:12, and 10:14 h L:D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under $5{\sim}10^{\circ}C$, 10 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D), whereas the conditions for male gametophytes were slightly different at $20^{\circ}C$, 40 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D). The sexual maturation of female and male gametophytes was facilitated at $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}s^{-1}$, 14:10 h (L:D). These results provide basic information for controlling the regeneration and maturation of the free-living gametophytes for artificial seed production of E. stolonifera.

Keywords

References

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