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Development of a Highly Active Fluorescence-Based Detector for Yeast G Protein-Coupled Receptor Ste2p

  • Hong, Jin Woo;Ahn, Hee Jun;Baek, Jee Su;Hong, Eun young;Jin, Dong Hoon;Khang, Yong Ho;Hong, Nam Joo
    • Journal of Microbiology and Biotechnology
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    • v.28 no.10
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    • pp.1589-1603
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    • 2018
  • Twenty analogs of $[Orn^6,D-Ala^9]{\alpha}-factor$ were synthesized and assayed for their biological activities: seven analogs of $[Orn^6,X^9]{\alpha}-factor$, seven analogs of $[X^6,D-Ala^9]{\alpha}-factor$, five analogs of $[X^5,X^6,D-Ala^9]{\alpha}-factor$, and native ${\alpha}-factor$ (X = amino acids). Their biological activities (halo, gene induction, and affinity) were measured using S. cerevisiae Y7925 and LM102 and compared with those of native ${\alpha}-factor$ (100%). G protein-coupled receptor was expressed in strain LM102 containing pESC-LEU-STE2 vector. $[Dap^6,D-Ala^9]{\alpha}-factor$ with weak halo activity (10%) showed the highest receptor affinity (> 230%) and the highest gene induction activity (167%). $[Arg^6,D-Ala^9]{\alpha}-factor$ showed the highest halo activity (2,000%). The number of active binding sites per cell (about 20,000 for strain LM102) was determined using a newly-designed fluorescence-based detector, $[Arg^6,D-Ala^9]{\alpha}-factor-Edan$, with high sensitivity (12,500-fold higher than the absorption-based detector $[Orn^6]{\alpha}-factor-[Cys]_3$).

Design of Two-Stage X-Band Power Amplifier Using GaN-HEMT (GaN-HEMT를 이용한 X-대역 이단 전력증폭기 설계)

  • Lee, Wooseok;Lee, Hwiseob;Park, Seungkuk;Lim, Wonseob;Han, Jaekyoung;Park, Kwanggun;Yang, Youngoo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.27 no.1
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    • pp.20-26
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    • 2016
  • This paper presents an X-band two-stage power amplifier using GaN-HEMT. Two-stage structure was adopted to take its high gain and simple inter-stage matching network. Based on a 3D EM simulation, the bond-wire inductance and the parasitic capacitance were predicted. By reducing bond-wire inductance, Q of the matching network is decreased and the bandwidth is improved. The implemented two-stage PA shows a power gain of more than 16 dB, saturated output power of more than 42.5 dBm, and a efficiency of more than 35 % in frequency range of 8.1~8.5 GHz with an operating voltage of 40 V.

MULTIVARIATE DISTRIBUTIONS WITH SELFDECOMPOSABLE PROJECTIONS

  • Sato, Ken-Iti
    • Journal of the Korean Mathematical Society
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    • v.35 no.3
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    • pp.783-791
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    • 1998
  • A random vector X on $R^{d}$ with the following properties is constructed: the distribution of X is infinitely divisible and not selfdecomposable, but every linear transformation of X to a lower-dimensional space has a selfdecomposable distribution.

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SUBTOURNAMENTS ISOMORPHIC TO W5 OF AN INDECOMPOSABLE TOURNAMENT

  • Belkhechine, Houmem;Boudabbous, Imed;Hzami, Kaouthar
    • Journal of the Korean Mathematical Society
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    • v.49 no.6
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    • pp.1259-1271
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    • 2012
  • We consider a tournament T = (V,A). For each subset X of V is associated the subtournament T(X) = (X,$A{\cap}(X{\times}X)$) of T induced by X. We say that a tournament T' embeds into a tournament T when T' is isomorphic to a subtournament of T. Otherwise, we say that T omits T'. A subset X of V is a clan of T provided that for a, $b{\in}X$ and $x{\in}V{\backslash}X$, $(a,x){\in}A$ if and only if $(b,x){\in}A$. For example, ${\emptyset}$, $\{x\}(x{\in}V)$ and V are clans of T, called trivial clans. A tournament is indecomposable if all its clans are trivial. In 2003, B. J. Latka characterized the class ${\tau}$ of indecomposable tournaments omitting a certain tournament $W_5$ on 5 vertices. In the case of an indecomposable tournament T, we will study the set $W_5$(T) of vertices $x{\in}V$ for which there exists a subset X of V such that $x{\in}X$ and T(X) is isomorphic to $W_5$. We prove the following: for any indecomposable tournament T, if $T{\notin}{\tau}$, then ${\mid}W_5(T){\mid}{\geq}{\mid}V{\mid}$ -2 and ${\mid}W_5(T){\mid}{\geq}{\mid}V{\mid}$ -1 if ${\mid}V{\mid}$ is even. By giving examples, we also verify that this statement is optimal.

Design of X-band Core Chip Using 0.25-㎛ GaAs pHEMT Process (0.25 ㎛ GaAs pHEMT 공정을 이용한 X-대역 코아-칩의 설계)

  • Kim, Dong-Seok;Lee, Chang-Dae;Lee, Dong-Hyun;Yeom, Kyung-Whan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.29 no.5
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    • pp.336-343
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    • 2018
  • We herein present the design and fabrication of a Rx core chip operating in the X-band (10.5~13 GHz) using Win's commercial $0.25-{\mu}m$ GaAs pHEMT process technology. The X-band core chip comprises a low-noise amplifier, a four-bit phase shifter, and a serial-to-parallel data converter. The size is $1.75mm{\times}1.75mm$, which is the state-of-the-art size. The gain and noise figure are more than 10 dB but less than 2 dB, and both the input and output return losses are less than 10 dB. The RMS phase error is less than $5^{\circ}$, and the P1dB is 2 dBm at 12.5 GHz, the performance of which is equivalent to other GaAs core chips. The fabricated core chip was packaged in a QFN package type with a size of $3mm{\times}3mm$ for the convenience of assembly. We confirmed that the performance of the packaged core chip was almost the same as that of the chip itself.

Strength Characteristics of 3D Printed Concrete According to the Stacking Direction (적층 방향에 따른 3D 프린팅 콘크리트의 강도 특성)

  • Won, Hee-Jae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.2
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    • pp.632-637
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    • 2021
  • In order to develop future construction technology, research is actively being conducted on concrete construction technology using 3D printing, which is currently in the spotlight as a future industry in domestic and foreign construction industries and academia. However, 3D printing technology is currently being developed and does not meet the requirements for proper construction technology and the properties of concrete materials, and it is difficult to apply in the actual field. Research is also needed for the durability management and maintenance of constructed structures. This work compares the compressive and flexural strength to that produced in conventional molds by dividing the 3D printed concrete output by the laminated X, Y, and Z axes. The compressive strength of a test specimen in the II Z-axis test direction was 8-10% higher than that of the other test directions (I and III Y axes and X axis). The strength was 4% lower than that of a molded test specimen. As of 28th of the age, the bending strength of the test specimen in the Z-axis direction was 5 to 7% higher than that of the I and III Y, and X-axis test directions, and the strength was 2% lower than that of the molded test specimen.

Study on a 3 Dimension Game Engine: Development of the Game ″3D Fortress″ (3D 게임 엔진 연구: ″3D 포트리스″ 게임 개발 사례)

  • 우석진;이동희;이동건;신일경;박승호;장효선;김호준;최승관;김경식
    • Proceedings of the Korea Multimedia Society Conference
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    • 2000.11a
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    • pp.463-466
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    • 2000
  • 온라인 3D 컴퓨터 게임에서 크게 대두되는 두 가지 기술 분야는 3D 연출 기술과 온라인 기술이다. 3D 연출 기술은 3D 가속기의 출현으로 더욱 발전을 할 수 있었고, Microsoft사의 DirectX가 나옴으로써 다양한 하드웨어를 좀더 쉽게 제어할 수 있게 되어 게임 산업은 한층 더 발전하게 되었다. 그러나 DirectXrl반의 3D 기술을 응용하여 게임 엔진을 만드는데는 게임의 특징에 따라 엔진 구성이 크게 차이가 나기 때문에 최종 게임 엔진까지는 사용자가 직접 만들어야 하는 어려움이 있다. 본 연구에서는 DirectX를 이용해서 3D 온라인 기반의 게임을 제작할 수 있는 3D 엔진을 구현하였고 포트리스 3D 라는 3차원 온라인 슈팅 게임의 개발에 적용한 사례를 발표한다.

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On a Question of Closed Maps of S. Lin

  • Chen, Huaipeng
    • Kyungpook Mathematical Journal
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    • v.50 no.4
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    • pp.537-543
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    • 2010
  • Let X be a regular $T_1$-space such that each single point set is a $G_{\delta}$ set. Denot 'hereditarily closure-preserving' by 'HCP'. To consider a question of closed maps of S. Lin in [6], we improve some results of Foged in [1], and prove the following propositions. Proposition 1. $D\;=\;\{x{\in}X\;:\;\mid\{F{\in}\cal{F}:x{\in}F\}\mid{\geq}{\aleph}_0\}$ is discrete and closed if $\cal{F}$ is a collection of HCP. Proposition 2. $\cal{H}\;=\;\{{\cup}\cal{F}'\;:\;F'$ is an fininte subcolletion of $\cal{F}_n\}$ is HCP if $\cal{F}$ is a collection of HCP. Proposition 3. Let (X,$\tau$) have a $\sigma$-HCP k-network. Then (X,$\tau$) has a $\sigma$-HCP k-network F = ${\cup}_n\cal{F}_n$ such that such tat: (i) $\cal{F}_n\;\subset\;\cal{F}_{n+1}$, (ii) $D_n\;=\;\{x{\in}X\;:\;\mid\{F{\in}\cal{F}_n\;:\;x{\in}F\}\mid\;{\geq}\;{\aleph}_0\}$ is a discrete closed set and (iii) each $\cal{F}_n$ is closed to finite intersections.

A study on fabrecation and characteristics of short channel SNOSFET EEPROM (Short channel SNOSFET EEPROM의 제작과 특성에 관한 연구)

  • 강창수;김동진;서광열
    • Electrical & Electronic Materials
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    • v.6 no.4
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    • pp.330-338
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    • 1993
  • Channel의 폭과 길이가 15 x 15.mu.m, 15 x 1.5.mu.m, 1.9 x 1.7.mu.m인 비휘발성 SNOSFET EEPROM 기억소자를 CMOS 1 Mbit 설계규칙에 의하여 제작하고 체널크기에 따른 $I_{D}$- $V_{G}$특성 및 스위칭 특성을 조사하여 비교하였다. 게아트에 전압을 인가하여 질화막에 전하를 주입시키거나 소거시킨 후 특성을 측정한 결과, 드레인전류가 적게 흐르는 저전도상태와 전류가 많이 흐르는 고전도상태로 되는 것을 확인하였다. 15 x 15.mu.m의 소자는 전형적인 long channel특성을 나타냈으며 15 x 1.5.mu.m, 1.9 x 1.7.mu.m는 short channel특성을 보였다. $I_{D}$- $V_{G}$ 특성에서 소자들의 임계 문턱전압은 저전도상태에서 $V_{W}$=+34V, $t_{W}$=50sec의 전압에서 나타났으며 메모리 윈도우 폭은 15 x 15.mu.m, 15 x 1.5.mu.m, 1.9 x 1.7.mu.m의 소자에서 각각 6.4V, 7.4V, 3.5V였다. 스위칭 특성조사에서 소자들은 모두 논리스윙에 필요한 3.5V 메모리 윈도우를 얻을 수 있었으며 논리회로설계에 적절한 정논리 전도특성을 가졌다.특성을 가졌다.다.다.

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ITERATIVE PROCESS FOR FINDING FIXED POINTS OF QUASI-NONEXPANSIVE MULTIMAPS IN CAT(0) SPACES

  • Pitchaya Kingkam;Jamnian Nantadilok
    • Korean Journal of Mathematics
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    • v.31 no.1
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    • pp.35-48
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    • 2023
  • Let 𝔼 be a CAT(0) space and K be a nonempty closed convex subset of 𝔼. Let T : K → 𝓟(K) be a multimap such that F(T) ≠ ∅ and ℙT(x) = {y ∈ Tx : d(x, y) = d(x, Tx)}. Define sequence {xn} by xn+1 = (1 - α)𝜈n⊕αwn, yn = (1 - β)un⊕βwn, zn = (1-γ)xn⊕γun where α, β, γ ∈ [0; 1]; un ∈ ℙT (xn); 𝜈n ∈ ℙT (yn) and wn ∈ ℙT (zn). (1) If ℙT is quasi-nonexpansive, then it is proved that {xn} converges strongly to a fixed point of T. (2) If a multimap T satisfies Condition(I) and ℙT is quasi-nonexpansive, then {xn} converges strongly to a fixed point of T. (3) Finally, we establish a weak convergence result. Our results extend and unify some of the related results in the literature.