• Journal of fish pathology
• /
• v.35 no.1
• /
• pp.65-75
• /
• 2022

Mirror carp, Cyprinus carpio nudus (weight 26.2±3.1 g, length 11.8±0.5 cm) were exposed to waterborne total ammonia nitrogen (TAN) at 0, 2, 4, 8, 16, 32, 64 and 128 mg TAN/L. The lethal concentration 50 (LC₅₀) of mirror carp, C. carpio nudus exposed to waterborne ammonia was 60.38 mg TAN/L. The red blood cell (RBC) count was significantly reduced by ammonia exposure, whereas there was no significant changes in the hemoglobin concentration and hematocrit value. In the plasma components, glucose, total protein, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased by ammonia exposure. The results of this study suggest that the ammonia exposure to C. carpio nudus affects the survival rates, hematological parameters and plasma components as toxicity.

• Korean Journal of Environmental Agriculture
• /
• v.6 no.2
• /
• pp.66-72
• /
• 1987

Sensitivity of the three freshwater fish, Cyprinus carpio, Oryzias latipes (wildtype indigenous to Korea), and O. latipes (Japanese killifish) to 30 pesticide formulations were studied in terms of 48 hr $LC_{50}$ determined with the static method. The correlation between C. carpio and O. latipes (Japanese killifish) was higher than that between C. carpio and O. latipes with correlation coefficients of 0.89 and 0.80, respectively. The sensitivity of O. latipes and O. latipes (Japanese killifish) to pesticides showed very high correlation with a coefficient of 0.93. Therefore, it is suggested that the acute toxicity data concerning O. latipes (Japanese killifish) could represent those C. carpio or O. latipes which are indigenous species in Korea. Also, it is found that the present protocol for the toxicity test with carp in Korea has difficulties in maintaining the proper concentration of dissolved oxygen in the test chamber because of the abrupt decrease of dissolved oxygen to 2mg/l, which is not acceptable according to general guidelines of foreign countries.

• Environmental Analysis Health and Toxicology
• /
• v.23 no.3
• /
• pp.171-178
• /
• 2008

수백여 종의 개별물질이 불완전 연소 혹은 유기물의 열분해로 인해 발생되는 다환방향족 탄화수소(PAHs)는 환경에서 중요한 오염원이 되고 있다. 본 연구는 다양한 바이오마커를 이용하여 수서생태계에 벤조피렌(benzo[a]pyrene)과 같은 다환방향족 탄화수소의 영향을 분석하였고, 이에 대한 통합적 결과 모델을 도출하였다. 즉, 잉어(Cyprinus carpio)를 이용하여 여러 농도의 벤조피렌(3, 12, $34{\mu}g/L$, 측정농도 기준)에 10일간 노출시킨 다음, DNA single-strand break, ethoxyresorufin-O-deethylase (EROD), acetylcholine esterase (AChE)와 vitellogenin (VTG)의 농도를 측정하였다. 벤조피렌은 잉어의 DNA 손상을 유도하였고, 낮은 농도에서 EROD와 VTC의 유의적인 활성을 보였으나, 신경전달물질과 관련이 깊은 AChE 효소활성에는 영향을 미치지 않았다. 이 결과를 star plot를 이용하여 통합 및 분석하였으며, 노출농도에 따른 통합 반응지수(integrated biomarker response value: IBR)로 나타내었다. 이런 다양한 바이오마커의 결과들은 벤조피렌에 대한 어류의 영향과 수생태 모니터링 자료로 이용 가능할 것으로 여겨지며, 통합반응지수는 생태위해성평가에서 유용한 도구로 쓰일 가치가 있는 것으로 평가된다.

• Journal of fish pathology
• /
• v.23 no.2
• /
• pp.165-176
• /
• 2010

In May 2007, mass mortality of Israeli carp, Cyprinus carpio L., was occured on a pond farm located in Jeollabukdo Province, Korea. The mortality rates reached up to 2% of the total fish in the farm per day. Typical clinical signs were abdominal distension, reddish foci on the skin, enteritis, liver congestion and enlarged spleen and kidney. On the basis of biochemical characteristics and 16S rDNA sequence, the causative bacteria isolated from affected carp were identified as Aeromonas veronii. Histologically, degeneration of hepatocytes and congestion in sinusoids were observed in the liver. Spleen showed hemorrhage and the destruction of the sheathed tissues. In kidney, necrotized renal tubules and glomerular destructions were observed. Intestinal tissues revealed necrotized and severe hemorrhage. Mass hemorrhage was observed in muscles. This is the first report that A. veronii caused mortality in cultured Israeli carp in Korea.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.54 no.6
• /
• pp.954-964
• /
• 2021

Mirror carp Cyprinus carpio nudus (weight 42.0±3.8 g, length 14.3±0.4 cm) were exposed to different concentrations of waterborne copper (0, 100, 200, 400, 800, and 1,600 ㎍ Cu²⁺·L^-1) at 20.3℃ for 96 h. The lethal concentration 50 of waterborne copper was 1,176.45 ㎍ Cu²⁺·L^-1. Among hematological parameters, red blood cell count was significantly decreased, whereas there were no significant changes in the hemoglobin concentration and hematocrit value. Among the inorganic plasma components, calcium was significantly decreased following copper exposure. Conversely, organic plasma components such as glucose and total protein were significantly increased. Similarly, enzymatic components, such as aspartate transaminase, alanine transaminase, and alkaline phosphatase, were also significantly increased. These findings suggest that the copper exposure is detrimental to the survival rates and physiology of C. carpio nudus.

• Journal of Applied Biological Chemistry
• /
• v.61 no.2
• /
• pp.151-155
• /
• 2018

In this study, cinnamon essential oil (CEO) was formulated as emulsifiable concentrate (EC) and a granule. For the evaluation of their negative effects on the ecosystem, acute toxicities against Cyprinus carpio was determined in a static condition. The formulations were made using CEOs extracted by 3 different methods (steam distillation (SD), solvent extraction and supercritical fluid extraction (SFE)) and were tested to obtain $LC_{50}$ values. Among the ECs, EC including CEO extracted by SFE showed highest acute toxicity against C. carpio. Among the granules, a granule including CEO extracted by SD showed highest acute toxicity against C. carpio. Nevertheless, $LC_{50}$ of EC and a granule formulation with CEOs was higher than toxicity level III of pesticide standardized by Korea rural development administration. These results were similar to those using zebrafishes. Chronic toxicities were not found for 45 days in zebrafishes until $500{\mu}gL^{-1}$ level of EC formulation including CEO obtained by the SD. Based on these results, EC formulation of CEOs may be considered to be used as environmental-friendly natural insecticides in accordance with the standards.

• The Korean Journal of Pesticide Science
• /
• v.11 no.4
• /
• pp.217-221
• /
• 2007

In order to elucidate the behavior of the fungicide hexaconazole (1-(6-chloro-3-pyrldyhnethyl)N-nitroimidazolidin-2-ylideneamine) in carp (Cyprinus carpio L.), carps were exposed to [$^{14}C$]hexaconazole at a predicted environmental concentration (PEC) of 0.32 mg $L^{-1}$ for 4 days under static conditions. Hexaconazole in water was absorbed into carps to reach the maximum concentration 2 days after exposure. The amounts of the [$^{14}C$]hexaconazole and its metabolites absorbed in gall were much higher than those in the other organs and especially those in gall 2 days after exposure were 25 and 67 times higher than those in liver and kidney, respectively, strongly suggesting that biliary excretion involving enterohepatic recirculation could be an import route for the elimination of hexaconazole absorbed in carps.

• Applied Biological Chemistry
• /
• v.36 no.1
• /
• pp.64-69
• /
• 1993

This study was performed to investigate detoxifying enzyme activities of carboxylesterase(CE), glutathione S-transferase(GST) and lactate dehydrogenase(LDH) at variable toxicity levels in fresh water fish, carp(Cyprinus carpio L.). The carp was exposed to single and combined pesticides of IBP, isoprothiolane and cartap for 48 hr at sublethal doses, $LC_{10}$ and $LC_{26}$. The detoxifying enzyme activities were assayed for the liver, head and gut of the carp. The enzyme activities we discovered were as follows: Both activities of CE and GST were increased at the sublethal doses but were declined by increasing doses. In the gut, we found that the CE activity had high levels in the treatment groups of isoprothiolane+IBP and isoprothiolane+cartap. In the head, the CE activity had high levels in the treatment groups of cartap, IBP and isoprothiolane. However, the GST activities were inconsistent in the head and gut of the fish. Also, the GST activity was declined by increasing protein contents. The highest LDH activity was shown in the isoprothiolane treated fish, while the lowest activity was observed in the isoprothiolane+cartap treatment.

• Korean Journal of Environmental Agriculture
• /
• v.14 no.2
• /
• pp.163-170
• /
• 1995

This study was carried out to compare the acute toxicity(96hr) of 13 chemicals to carp (Cyprinus carpio L.) and israeli carp (Cyprinus israeli carpio L.) and the activities of acetylcholinesterase (AchE) and glutathione S-transferase (GST) in israeli carp exposed to five insecticides (diazinon, malathion, carbofuran, cartap, methomyl). $LC_{50}$ values of acute toxicity of the chemicals to israeli carp were endosulfan 0.0061ppm, captafol 0.041ppm, chlorothalonil 0.073ppm, butachlor 0.48ppm, captan 0.14ppm, carbofuran 1.13ppm, cartap 1.15ppm, diazinon 1.35ppm, nitrofen 3.72ppm, methomyl 4.39ppm, propanil 10.61ppm, malathion 11.78ppm and isoprothiolane 12.81ppm. The acute toxicity of endosulfan 0.0061ppm was 2100 times higher than that of isoprothiolane 12.81ppm. $LC_{50}$ values of acute toxicity of the chemicals to carp were endosulfan 0.0026ppm, captafol 0.062ppm, chlorothalonil 0.078ppm, captan 0.14ppm, and butachlor 0.47ppm, carbofuran 0.52ppm, nitrofen 0.58ppm, diazinon 0.81ppm, cartap 0.82ppm, methomyl 5.03ppm, propanil 10.67ppm, malathion 11.92ppm, and isoprothiolane 13.20ppm. The acute toxicity of endosulfan was 5,000 times higher than that of isoprothiolane. The toxicity of diazinon, carbofuran, cartap, endosulfan, and nitrofen to carp was approximately 2-6 times as high as that to israeli carp, but the toxicity of malathion, methomyl and captafol to israeli carp was slightly higher than that to carp. AchE activity was inhibited by 31% and 52% after 96hr’s exposure of israeli carp to diazinon and malathion respectively. GST activity in israeli carp was significantly induced by methomyl exposure for 96 hr.

• Korean Journal of Environmental Biology
• /
• v.22 no.1
• /
• pp.111-119
• /
• 2004

The ichthyofauna and community structure in Lake Paro, Korea, was investigated from December 2002 to september 2003. Buying the surveyed period 45 species belonging 12 families were collected. There were 15 Korean endemic species 33.33％, including Hemibarbus mylodon, Aeheilognathus yamatsutae nnd Ooreoperca hem. Dominant species were Zacco platypus (72.41％) and subdominant species were Hemibarbus labeo (9.75％). Also, Opsariichthys uncirostris amurensis (5.98％), Zacco temmincki (2.42％), Rhinogobius brunneus (2.01％) and Hypomesus olidus (0.88％) were numerous. The biomass of collected fishes were Zacco platypus (82,803.15 g), Hemibarbus labeo (32,942.63 g), Opsariichthys uncirostris amurensis (27,482.39 g), Cyprinus carpio (6,502.87 g), Anguilla japonica (3,022.15 g) and Siniperca scherzeri (2,711.05 g). Of the 10 introduced fishes in Lake Paro Cyprinus carpio (Israeli type), Carassius cuvieri, Micropterus salmoides and Lepomis macrochirus were originated form foreign countries but Anguillta japonica, Hemiculter eigenmanni, Hypomesus olidus, Oncorhynchus masou mason, Chaenogobius urotaenius, Rhinogobius giurinus were introduced from other native river systems.