Browse > Article
http://dx.doi.org/10.7847/jfp.2022.35.1.065

Effects on lethal concentration 50%, hematological parameters and plasma components of mirror carp, Cyprinus carpio nudus exposed to waterborne ammonia  

Jeong, Ji-Ho (Sun Moon University, Department of Aquatic Life and Medical Science)
Eun, Ji-Su (Sun Moon University, Department of Aquatic Life and Medical Science)
Joo, Chang-Hoon (Sun Moon University, Department of Aquatic Life and Medical Science)
Jo, A-Hyun (Sun Moon University, Department of Aquatic Life and Medical Science)
Hong, Su-Min (Sun Moon University, Department of Aquatic Life and Medical Science)
Kim, Jun-Hwan (Sun Moon University, Department of Aquatic Life and Medical Science)
Publication Information
Journal of fish pathology / v.35, no.1, 2022 , pp. 65-75 More about this Journal
Abstract
Mirror carp, Cyprinus carpio nudus (weight 26.2±3.1 g, length 11.8±0.5 cm) were exposed to waterborne total ammonia nitrogen (TAN) at 0, 2, 4, 8, 16, 32, 64 and 128 mg TAN/L. The lethal concentration 50 (LC50) of mirror carp, C. carpio nudus exposed to waterborne ammonia was 60.38 mg TAN/L. The red blood cell (RBC) count was significantly reduced by ammonia exposure, whereas there was no significant changes in the hemoglobin concentration and hematocrit value. In the plasma components, glucose, total protein, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased by ammonia exposure. The results of this study suggest that the ammonia exposure to C. carpio nudus affects the survival rates, hematological parameters and plasma components as toxicity.
Keywords
Ammonia exposure; $LC_{50}$; Hematological parameters; Plasma components; Mirror carp;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Das, P.C., Ayyappan, S., Jena, J.K. and Das, B.K.: Acute toxicity of ammonia and its sub-lethal effects on selected haematological and enzymatic parameters of mrigal, Cirrhinus mrigala (Hamilton). Aquac Res, 35:134-143, 2004.   DOI
2 Debbarma, R., Biswas, P. and Singh, S.K.: An integrated biomarker approach to assess the welfare status of Ompok bimaculatus (Pabda) in biofloc system with altered C/N ratio and subjected to acute ammonia stress. Aquaculture, 545:737184, 2021.   DOI
3 Deguchi, Y., Toyoizumi, T., Masuda, S., Yasuhara, A., Mohri, S., Yamada, M. and Kinae, N.: Evaluation of mutagenic activities of leachates in landfill sites by micronucleus test and comet assay using goldfish. Mutat Res Genet Toxicol Environ Mutagen, 627:178-185, 2007.   DOI
4 do Carmo Neves, L., e Silva, W.D.S., Ferreira, A.L., Favero, G.C., Beier, S.L., Palheta, G.D.A. and Luz, R.K.: Physiological responses of juvenile Colossoma macropomum after different periods of air exposure. Aquaculture, 548:737583, 2022.   DOI
5 Fan, B., Li, J., Wang, X., Chen, J., Gao, X., Li, W. and Liu, Z.: Ammonia spatiotemporal distribution and risk assessment for freshwater species in aquatic ecosystem in China. Ecotoxicol Environ Saf, 207:111541, 2021.   DOI
6 Forouhar Vajargah, M., Imanpoor, M.R., Shabani, A., Hedayati, A. and Faggio, C.: Effect of long-term exposure of silver nanoparticles on growth indices, hematological and biochemical parameters and gonad histology of male goldfish (Carassius auratus gibelio). Microsc Res Tech, 82:1224-1230, 2019.   DOI
7 Hoseini, S.M., Yousefi, M., Hoseinifar, S.H. and Van Doan, H.: Antioxidant, enzymatic and hematological responses of common carp (Cyprinus carpio) fed with myrcene-or menthol-supplemented diets and exposed to ambient ammonia. Aquaculture, 506:246-255, 2019.   DOI
8 Gu, P., Li, Q., Zhang, W., Gao, Y., Sun, K., Zhou, L. and Zheng, Z.: Biological toxicity of fresh and rotten algae on freshwater fish: LC50, organ damage and antioxidant response. J Hazard Mater, 407:124620, 2021.   DOI
9 Harikrishnan, R., Balasundaram, C. and Heo, M.S.: Herbal supplementation diets on hematology and innate immunity in goldfish against Aeromonas hydrophila. Fish Shellfish Immunol, 28:354-361, 2010.   DOI
10 Hoseini, S.M., Mirghaed, A.T., Iri, Y., Hoseinifar, S.H., Van Doan, H. and Reverter, M.: Effects of dietary Russian olive, Elaeagnus angustifolia, leaf extract on growth, hematological, immunological, and antioxidant parameters in common carp, Cyprinus carpio. Aquaculture, 536:736461, 2021.   DOI
11 Iftikhar, N. and Hashmi, I.: Assessment of immunohematological, hematological and biochemical responses in cultivable fish Cyprinus carpio exposed to an antibiotic sulfamethoxazole (SMX). J Water Health, 19:108-119, 2021.   DOI
12 Jee, J.H., Masroor, F. and Kang, J.C.: Responses of cypermethrin- induced stress in haematological parameters of Korean rockfish, Sebastes schlegeli (Hilgendorf). Aquac Res, 36:898-905, 2005.   DOI
13 Abbas, H.H.: Acute toxicity of ammonia to common carp fingerlings (Cyprinus carpio) at different pH levels. Pak J Biol Sci 9: 2215-2221, 2006.   DOI
14 Ananias, I.D.M.C., de Melo, C.L., Costa, D.C., Ferreira, A.L., Martins, E.D.F.F., Takata, R. and Luz, R.K.: Menthol as anesthetic for juvenile Lophiosilurus alexandri: Induction and recovery time, ventilatory frequency, hematology and blood biochemistry. Aquaculture, 546:737373. 2022.   DOI
15 Aranda-Morales, S.A., Pena-Marin, E.S., Jimenez-Martinez, L.D., Martinez-Burguete, T., Martinez-Bautista, G., Alvarez-Villagomez, C.S. and Alvarez-Gonzalez, C.A.: Expression of ion transport proteins and routine metabolism in juveniles of tropical gar (Atractosteus tropicus) exposed to ammonia. Comp Biochem Physiol C Toxicol Pharmacol, 250:109166, 2021.   DOI
16 Yu, H., Yang, L., Li, D. and Chen, Y.: A hybrid intelligent soft computing method for ammonia nitrogen prediction in aquaculture. Inf Process Agric, 8:64-74, 2021.
17 Barbieri, E. and Bondioli, A.C.V.: Acute toxicity of ammonia in Pacu fish (Piaractus mesopotamicus, Holmberg, 1887) at different temperatures levels. Aquac Res, 46:565-571, 2015.   DOI
18 Yang, W., Xiang, F., Sun, H., Chen, Y., Minter, E. and Yang, Z.: Changes in the selected hematological parameters and gill Na+/K+ ATPase activity of juvenile crucian carp Carassius auratus during elevated ammonia exposure and the post-exposure recovery. Biochem Syst Ecol, 38:557-562, 2010.   DOI
19 Yousefi, M., Abtahi, B., Adineh, H., Hoseinifar, S.H., Taheri Mirghaed, A., Paolucci, M. and Van Doan, H.: Effects of dietary arginine supplementation on cytokine-and antioxidant-related gene expressions in common carp (Cyprinus carpio) fingerling during ammonia toxicity. Aquac Res, 52:2751-2758, 2021b.   DOI
20 Bhakta, J.N.: Ammonia toxicity to four freshwater fish species: Catla catla, Labeo bata, Cyprinus carpio and Oreochromis mossambica. eJBio, 2:39-41, 2006.
21 Kavitha, C., Malarvizhi, A., Kumaran, S.S. and Ramesh, M.: Toxicological effects of arsenate exposure on hematological, biochemical and liver transaminases activity in an Indian major carp, Catla catla. Food Chem Toxicol, 48:2848-2854, 2010.   DOI
22 Kim, J.H., Park, H.J., Hwang, I.K., Han, J.M., Kim, D. H., Oh, C.W. and Kang, J.C.: Toxic effects of juvenile sablefish, Anoplopoma fimbria by ammonia exposure at different water temperature. Environ Toxicol Pharmacol, 54:169-176, 2017b.   DOI
23 Yousefi, M., Vatnikov, Y.A., Kulikov, E.V., Ahmadifar, E., Mirghaed, A.T., Hoseinifar, S.H. and Van Doan, H.: Effects of dietary Hibiscus sabdariffa supplementation on biochemical responses and inflammatory- related genes expression of rainbow trout, Oncorhynchus mykiss, to ammonia toxicity. Aquaculture, 533:736095, 2021a.   DOI
24 Kumar, R. and Banerjee, T.K.: Arsenic induced hematological and biochemical responses in nutritionally important catfish Clarias batrachus (L.). Toxicol Rep, 3:148-152, 2016.   DOI
25 Lee, G.H., Harwanto, D., Park, S.M., Choi, J.S., Kim, M.R. and Hong, Y.K.: Hot water extract of leather carp (Cyprinus carpio nudus) improves exercise performance in mice. Prev Nutr Food Sci, 20:246, 2015.   DOI
26 Magouz, F.I., Mahmoud, S.A., El-Morsy, R.A., Paray, B.A., Soliman, A.A., Zaineldin, A.I. and Dawood, M.A.: Dietary menthol essential oil enhanced the growth performance, digestive enzyme activity, immune- related genes, and resistance against acute ammonia exposure in Nile tilapia (Oreochromis niloticus). Aquaculture, 530:735944, 2021.   DOI
27 Zhao, L., Cui, C., Liu, Q., Sun, J., He, K., Adam, A. A. and Yang, S.: Combined exposure to hypoxia and ammonia aggravated biological effects on glucose metabolism, oxidative stress, inflammation and apoptosis in largemouth bass (Micropterus salmoides). Aquat Toxicol, 224:105514, 2020.   DOI
28 Zeitoun, M.M., EL-Azrak, K.E.D.M., Zaki, M.A., Nemat- Allah, B.R. and Mehana, E.S.E.: Effects of ammonia toxicity on growth performance, cortisol, glucose and hematological response of Nile Tilapia (Oreochromis niloticus). Aceh J Animal Sci, 1:21-28, 2016.   DOI
29 Zhang, M., Li, M., Wang, R. and Qian, Y.: Effects of acute ammonia toxicity on oxidative stress, immune response and apoptosis of juvenile yellow catfish Pelteobagrus fulvidraco and the mitigation of exogenous taurine. Fish Shellfish Immunol, 79:313-320, 2018.   DOI
30 Zhao, H., Peng, K., Wang, G., Mo, W., Huang, Y. and Cao, J.: Metabolic changes, antioxidant status, immune response and resistance to ammonia stress in juvenile yellow catfish (Pelteobagrus fulvidraco) fed diet supplemented with sodium butyrate. Aquaculture, 536:736441, 2021.   DOI
31 Shalaby, A.M., Khames, M.K., Fathy, A., Gharieb, A.A. and Abdel-Hamid, E.A.: The Impact of Zeolite on Ammonia Toxicity, Growth Performance and Physiological Status of the Nile Tilapia (Oreochromius niloticus). Egypt J Aquat Biol Fish, 25:643-663, 2021.
32 Prakash, S. and Verma, A.K.: Effect of arsenic on lipid metabolism of a fresh water cat fish, Mystus vittatus. Joural of Fisheries and Life Sciences, 4: 33-35, 2019.
33 Mangang, Y.A. and Pandey, P.K.: Hemato-biochemical responses and histopathological alterations in the gill and kidney tissues of Osteobrama belangeri (Valenciennes, 1844) exposed to different sub-lethal unionized ammonia. Aquaculture, 542:736887, 2021.   DOI
34 Molayemraftar, T., Peyghan, R., Jalali, M.R. and Shahriari, A.: Single and combined effects of ammonia and nitrite on common carp, Cyprinus carpio: Toxicity, hematological parameters, antioxidant defenses, acetylcholinesterase, and acid phosphatase activities. Aquaculture, 548:737676, 2022.   DOI
35 Paterson, B.D., Rimmer, M.A., Meikle, G.M. and Semmens, G.L.: Physiological responses of the Asian sea bass, Lates calcarifer to water quality deterioration during simulated live transport: acidosis, red-cell swelling, and levels of ions and ammonia in the plasma. Aquaculture, 218:717-728, 2003.   DOI
36 Seriani, R., de Souza Abessa, D.M., Kirschbaum, A.A., Pereira, C.D. S., Romano, P. and Ranzani-Paiva, M.J.T.: Relationship between water toxicity and hematological changes in Oreochromis niloticus. Braz J Aquat Sci Tech, 15:47-53, 2011.
37 Shin, K.W., Kim, S.H., Kim, J.H., Hwang, S.D. and Kang, J.C.: Toxic effects of ammonia exposure on growth performance, hematological parameters, and plasma components in rockfish, Sebastes schlegelii, during thermal stress. Fish Aquatic Sci, 19:1-8, 2016.   DOI
38 Shokr, E.S.A.: Effect of ammonia stress on blood constitutes in Nile tilapia. Egyptian Academic Journal of Biological Sciences, B. Zoology, 7:37-44, 2015.   DOI
39 Wilkie, M.P.: Mechanisms of ammonia excretion across fish gills. Comp Biochem Physiol A Physiol, 118:39-50, 1997.   DOI
40 Liu, M.J., Guo, H.Y., Zhu, K.C., Liu, B.S., Liu, B., Guo, L. and Zhang, D.C.: Effects of acute ammonia exposure and recovery on the antioxidant response and expression of genes in the Nrf2-Keap1 signaling pathway in the juvenile golden pompano (Trachinotus ovatus). Aquat Toxicol, 240:105969, 2021.   DOI
41 Carneiro, W.F., Castro, T.F.D., Reichel, T., de Castro Uzeda, P.L., Martinez-Palacios, C.A. and Murgas, L.D.S.: Diets containing Arthrospira platensis increase growth, modulate lipid metabolism, and reduce oxidative stress in pacu (Piaractus mesopotamicus) exposed to ammonia. Aquaculture, 547:737402, 2022.   DOI
42 Wright, P.A. and Wood, C.M.: Seven things fish know about ammonia and we don't. Respir Physiol Neurobiol, 184:231-240, 2012.   DOI
43 Gao, X.Q., Fei, F., Huang, B., Meng, X.S., Zhang, T., Zhao, K.F. and Liu, B.L.: Alterations in hematological and biochemical parameters, oxidative stress, and immune response in Takifugu rubripes under acute ammonia exposure. Comp Biochem Physiol C Toxicol Pharmacol, 243:108978, 2021.   DOI
44 Kim, J.H., Park, H.J., Hwang, I.K., Han, J.M., Kim, D. H., Oh, C.W. and Kang, J.C.: Alterations of growth performance, hematological parameters, and plasma constituents in the sablefish, Anoplopoma fimbria depending on ammonia concentrations. Fish Aquatic Sci, 20:1-6, 2017a.   DOI
45 Boudreaux, P.J., Ferrara, A.M. and Fontenot, Q.C.: Acute toxicity of ammonia to spotted gar, Lepisosteus oculatus, alligator gar, Atractosteus spatula, and paddlefish, Polyodon spathula. J WorldAquac Soc, 38:322-325, 2007.
46 Bu-Olayan, A.H. and Thomas, B.V.: Trace metals toxicity and bioaccumulation in mudskipper Periophthalmus waltoni Koumans 1941 (Gobiidae: Perciformes). Turk J Fish Aquat Sci, 8: 215-218, 2008.