Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Effect Evaluation of Benzo[a]pyrene on Multiple Biomarkers in Common Carp (Cyprinus carpio)

잉어 (Cyprinus carpio)의 다중바이오마커를 이용한 Benzo[a]pyrene의 영향평가

  • Kim, Woo-Keun (Environmental Toxicology Team, Korea Institute of Toxicology) ;
  • Kim, Ja-Hyun (Division of Non-Clinical Studies, Korea Institute of Toxicology) ;
  • Yeom, Dong-Hyuk (Division of Non-Clinical Studies, Korea Institute of Toxicology) ;
  • Lee, Sung-Kyu (Environmental Toxicology Team, Korea Institute of Toxicology)
  • 김우근 (안정성평가연구소 환경독성연구팀) ;
  • 김자현 (안전성시험본부) ;
  • 염동혁 (안전성시험본부) ;
  • 이성규 (안정성평가연구소 환경독성연구팀)
  • Published : 2008.09.30
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Abstract

수백여 종의 개별물질이 불완전 연소 혹은 유기물의 열분해로 인해 발생되는 다환방향족 탄화수소(PAHs)는 환경에서 중요한 오염원이 되고 있다. 본 연구는 다양한 바이오마커를 이용하여 수서생태계에 벤조피렌(benzo[a]pyrene)과 같은 다환방향족 탄화수소의 영향을 분석하였고, 이에 대한 통합적 결과 모델을 도출하였다. 즉, 잉어(Cyprinus carpio)를 이용하여 여러 농도의 벤조피렌(3, 12, $34{\mu}g/L$, 측정농도 기준)에 10일간 노출시킨 다음, DNA single-strand break, ethoxyresorufin-O-deethylase (EROD), acetylcholine esterase (AChE)와 vitellogenin (VTG)의 농도를 측정하였다. 벤조피렌은 잉어의 DNA 손상을 유도하였고, 낮은 농도에서 EROD와 VTC의 유의적인 활성을 보였으나, 신경전달물질과 관련이 깊은 AChE 효소활성에는 영향을 미치지 않았다. 이 결과를 star plot를 이용하여 통합 및 분석하였으며, 노출농도에 따른 통합 반응지수(integrated biomarker response value: IBR)로 나타내었다. 이런 다양한 바이오마커의 결과들은 벤조피렌에 대한 어류의 영향과 수생태 모니터링 자료로 이용 가능할 것으로 여겨지며, 통합반응지수는 생태위해성평가에서 유용한 도구로 쓰일 가치가 있는 것으로 평가된다.

Keywords

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