• Journal of Microbiology and Biotechnology
• /
• 제17권11호
• /
• pp.1868-1874
• /
• 2007

We have developed a robotic system for an automated parallel cell cultivation process that enables screening of induction parameters for the soluble expression of recombinant protein. The system is designed for parallelized and simultaneous cultivation of up to 24 different types of cells or a single type of cell at 24 different conditions. Twenty-four culture vessels of about 200 ml are arranged in four columns${\times}$six rows. The system is equipped with four independent thermostated waterbaths, each of which accommodates six culture vessels. A two-channel liquid handler is attached in order to distribute medium from the reservoir to the culture vessels, to transfer seed or other reagents, and to take an aliquot from the growing cells. Cells in each vessel are agitated and aerated by sparging filtered air. We tested the system by growing Escherichia coli BL21(DE3) cells harboring a plasmid for a model protein, and used it in optimizing protein expression conditions by varying the induction temperature and the inducer concentration. The results revealed the usefulness of our custom-made cell cultivation robot in screening optimal conditions for the expression of soluble proteins.

• International Journal of Computer Science & Network Security
• /
• 제22권10호
• /
• pp.207-213
• /
• 2022

The article reveals the problems of aesthetic education of young people as a necessary condition for the cultural development of the individual in the modern conditions of the information society. Aesthetic education should contribute to the formation of a creatively active personality. The basis of aesthetic education of young people in the modern conditions of the information society is the core of artistic culture - art as a unique form of public consciousness that contributes to the spiritual development and improvement of the inner world of a person. The main tasks of aesthetic education are highlighted. It is focused on the formation of aesthetic consciousness and aesthetic behavior of the individual. The formation of true aesthetic and spiritual values of students is impossible without a deep awareness of the national foundations of culture, which combines science (including technology), education, art, morality, way of life and worldview, and most importantly its information component - information culture. The effectiveness of aesthetic education of students largely depends on the skillful use of various methods and means by teachers. Aesthetic education of students involves a qualitative change in the level of their aesthetic culture in the modern conditions of the information society. In the era of information and computer technologies, the main Institute for aesthetic education of young people, as a necessary condition for the cultural development of the individual, is mass media. Television stands out especially because it has several information series (audio and video sequence), multiplied by the efficiency of providing information that increases several times compared to paper media, which allows you to report directly during the event.

• ALGAE
• /
• 제38권4호
• /
• pp.283-294
• /
• 2023

Previous research indicated that free-living sporangial filament keep hollow morph under high-culture density and form bipartite cells under low-culture density, while the following conchospore release was inhibited by high light. Here, we further explored the molecular bases of these affects caused by light and culture density using a transcriptome analysis. Many differentially expressed genes (DEGs) related to carbon dioxide concentration and fixation, photosynthesis, chlorophyll synthesis and nitrogen absorption were upregulated under high-light conditions compared with low-light conditions, indicating the molecular basis of rapid vegetative growth under the former. The stress response- and ion transport-related DEGs, as well as the gene encoding the vacuole formation-brefeldin A-inhibited guanine nucleotide exchange protein (BIG, py05721), were highly expressed under high-density conditions, indicating the molecular basis of the hollow morph of free-living sporangial filaments under high-culture density conditions. Additionally, the brefeldin A treatment indicated that the hollow morph was directly influenced by vacuole formation-related vesicle traffic. Others DEGs related to cell wall components, zinc-finger proteins, ASPO1527, cell cycle and cytoskeleton were highly expressed in the low density with low-light group, which might be related to the formation and release of conchospores. These results provide a deeper understanding of sporangial filaments in Neopyropia yezoensis and related species.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 1999년도 춘계 학술대회지
• /
• pp.20-21
• /
• 1999

• 과학기술학연구
• /
• 제1권1호
• /
• pp.5-27
• /
• 2001

이 글은 한국의 과학문화를 역사적으로 서술하려는 것이 아니라 앞으로 한국인이 지향하여야 하는 과학문화의 재구성을 위한 예비적 시론을 하고자 한다. 그 작업에 착수하기 위해서는 과학문화의 개념부터 규정해야 하므로 기존의 과학문화 개념들을 분류하고 이를 기반으로하여 인간화된 과학문화(Humanized Science Culture)를 잠정적 목표로 제시한다. 그 구체적인 사례 연구로서는 2061 프로젝트에 나타난 과학문화인(Science Literacy)의 개념을 분석하고 실제로 한국의 과학문화를 설계할 사람인 KDSC(Korean Designer of Science Culture)를 상정한다. KDSC는 자연인이 아니라 바람직한 한국인을 대표할 이성적 인간(理人)을 지칭한다. 따라서 KDSC가 될 수 있는 조건을 아울러 제시하고 그 요건들의 철학적 기반을 음미한다. 끝으로 KDSC의 시각에서 한국의 과학문화를 설계하고 실현하기 위한 프로그램을 개발하는데 필요할 것으로 생각되는 몇 가지를 제시한다.

• Clinical and Experimental Reproductive Medicine
• /
• 제47권4호
• /
• pp.284-292
• /
• 2020

Objective: This study investigated whether adding outer-well medium to inhibit osmotic changes in culture media in a dry-type incubator improved the clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) cycles. Methods: In culture dishes, the osmotic changes in media (20 µL)-covered oil with or without outer-well medium (humid or dry culture conditions, respectively) were compared after 3 days of incubation in a dry-type incubator. One-step (Origio) and G1/G2 (Vitrolife) media were used. Results: The osmotic changes in the dry culture condition (308 mOsm) were higher than in the humid culture conditions (285-290 mOsm) after 3 days of incubation. In day 3 IVF-ET cycles, although the pregnancy rate did not significantly differ between the dry (46.2%) and humid culture (51.0%) groups, the rates of abortion and ongoing pregnancy were significantly better in the humid culture group (1.5% and 49.5%, respectively) than in the dry culture group (8.3% and 37.8%, respectively, p<0.05). In day 5 IVF-ET cycles, the abortion rate was significantly lower in the humid culture group (2.2%) than in the dry culture group (25.0%, p<0.01), but no statistically significant difference was observed in the rates of clinical and ongoing pregnancy between the dry (50.0% and 25.0%, respectively) and humid culture groups (59.5% and 57.3%, respectively) because of the small number of cycles. Conclusion: Hyperosmotic changes in media occurred in a dry-type incubator by evaporation, although the medium was covered with oil. These osmotic changes were efficiently inhibited by supplementation of outer-well medium, which resulted in improved pregnancy outcomes.

• Fisheries and Aquatic Sciences
• /
• 제5권2호
• /
• pp.141-143
• /
• 2002

The treatment efficacy of oral administration of praziquantel against the blood-sucking monogenean Microcotyle sebastis under the commercial rockfish-culture conditions was evaluated. The quantity of praziquantel added to the food was calculated so that the amount of praziquantel ingested daily by fish was 200mg or 400mg/kg body weight (bw). The fish were fed a moist-pelleted fish meal supplemented with praziquantel at an rate of 0 (control), 200 or 400 mg of praziquantel per kg bw, respectively, for 3 times at an interval of 24 h. On 1 day to 8 days after the treatment, 10 fish were taken randomly from each net-pen daily (80 fish in each group in total), and the efficacy of each treatment was confirmed. Abundance of worms were significantly reduced in groups fed praziquantel-supplemented diets. The results clearly demonstrated that feeding the praziquantel-supplemented diet was effective for controlling M. sebastis infestation in practical commercial rockfish-culture systems without imposing any handling stress.

• Journal of Microbiology and Biotechnology
• /
• 제23권6호
• /
• pp.802-810
• /
• 2013

This work aims at investigating the production of yellow, orange, and red natural colorants in a submerged culture of Penicillium purpurogenum DPUA 1275. For this purpose, different experimental conditions evaluating the effect of incubation time, type and size of inoculum, and different carbon and nitrogen sources were performed. Furthermore, the growth kinetics were obtained in the conditions of $10^8$ spores/ml and 5 mycelia agar discs during 360 h. These experiments showed that 5 mycelia agar discs and 336 h promoted the highest yellow (3.08 $UA_{400nm}$), orange (1.44 $UA_{470nm}$), and red (2.27 $UA_{490nm}$) colorants production. Moreover, sucrose and yeast extract were the most suitable carbon and nitrogen sources for natural colorants production. Thus, the present study shows a new source of natural colorants, which can be used as an alternative to others available in the market after toxicological studies.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제7권2호
• /
• pp.89-94
• /
• 2002

The continuous production of citric acid from dairy wastewater was investigated using calcium-alginate immobilized Asrergillus niger ATCC 9142. The citric acid productivity and yield were strongly affected by the culture conditions. The optimal pH, temperature, and dilution rate were 3.0, 30$^{\circ}C$, and 0.025 h$\^$-1/, respectively. Under optimal culture conditions, the maximum productivity, concentration, and yield of citric acid produced by the calcium-alginate immobilized Aspergillus niger were 160 mg L$\^$-1/ h$\^$-1/, 4.5 g/L, and 70.3%, respectively, The culture was continuously perfored for 20 days without any apparent loss in citric acid productivity. Conversely, under the same conditions with a batch shake-flask culture, the maximum productivity, citric acid concentration, and yield were only 63.3 mg L$\^$-1/h$\^$-1/, 4.7 g/L and 51.4%, respectively, Therefore, the results suggest that the bioreactor used in this study could be potentially used for continuous citric acid production from dairy wastewater by applying calcium-alginate immobilized Aspergillus niger.

• Journal of Microbiology and Biotechnology
• /
• 제17권2호
• /
• pp.195-201
• /
• 2007

This study investigated the effects of various culture parameters (carbon sources, temperature, initial pH of culture, NaCl concentration, and light) on the growth and canthaxanthin production by Dietzia natronolimnaea HS-1. The results showed that the most effective carbon source for growth and canthaxantin production was glucose, and the best pH and temperature were 7 and $31^{\circ}C$, respectively. In addition, the biomass and canthaxanthin production increased in a medium without NaCl and in the presence of light. Under the optimized conditions, the maximum biomass, total carotenoid, and canthaxanthin production were $6.12{\pm}0.21g/l,\;4.51{\pm}0.20mg/l,\;and\;4.28{\pm}0.15mg/l$, respectively, in an Erlenmeyer flask system, yet increased to 7.25 g/l, 5.48 mg/l, and 5.29 mg/l, respectively, in a batch fermenter system.