• ALGAE
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• 제32권2호
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• pp.139-153
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• 2017

The ability of a red tide species to take up nutrients is a critical factor affecting its red tide dynamics and species competition. Nutrient uptake by red tide species has been conventionally measured by incubating nutrient-depleted cells for a short period at 1 or 2 light intensities. This method may be applicable to certain conditions under which cells remain in oligotrophic water for a long time and high nutrients are suddenly introduced. Thus, a new method should be developed that can be applicable to the conditions under which cells are maintained in eutrophicated waters in healthy conditions and experience light and dark cycles and different light intensities during vertical migration. In this study, a new repletion method reflecting these conditions was developed. The nitrate uptake rates of the red tide dinoflagellate Prorocentrum micans originally maintained in nitrate repletion and depletion conditions as a function of nitrate concentration were measured. With increasing light intensity from 10 to $100{\mu}E\;m^{-2}s^{-1}$, the maximum nitrate uptake rate ($V_{max}$) of P. micans increased from 3.6 to $10.8 pM\;cell^{-1}d^{-1}$ and the half saturation constant ($K_{s-NO3}$) increased from 4.1 to $6.9{\mu}M$. At $20{\mu}E\;m^{-2}s^{-1}$, the $V_{max}$ and $K_{s-NO3}$ of P. micans originally maintained in a nitrate repletion condition were similar to those maintained in a nitrate depletion condition. Thus, differences in cells under nutrient repletion and depletion conditions may not affect $K_{s-NO3}$ and $V_{max}$. Moreover, different light intensities may cause differences in the nitrate uptake of migratory phototrophic dinoflagellates.

• 한국환경보건학회지
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• 제39권5호
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• pp.391-407
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• 2013

Micropollutants emerge in surface water through untreated discharge from sewage and wastewater treatment plants (STPs and WWTPs). Most micropollutants resist the conventional systems in place at water treatment plants (WTPs) and survive the production of tap water. In particular, pharmaceuticals and endocrine disruptors (ECDs) are micropollutants frequently detected in drinking water. In this review, we summarized the distribution of micropollutants at WTPs and also scrutinized the effectiveness and mechanisms for their removal at each stage of drinking water production. Micropollutants demonstrated clear concentrations in the final effluents of WTPs. Although chronic exposure to micropollutants in drinking water has unclear adverse effects on humans, peer reviews have argued that continuous accumulation in water environments and inappropriate removal at WTPs has the potential to eventually affect human health. Among the available removal mechanisms for micropollutants at WTPs, coagulation alone is unlikely to eliminate the pollutants, but ionized compounds can be adsorbed to natural particles (e.g. clay and colloidal particles) and metal salts in coagulants. Hydrophobicities of micropollutants are a critical factor in adsorption removal using activated carbon. Disinfection can reduce contaminants through oxidation by disinfectants (e.g. ozone, chlorine and ultraviolet light), but unidentified toxic byproducts may result from such treatments. Overall, the persistence of micropollutants in a treatment system is based on the physico-chemical properties of chemicals and the operating conditions of the processes involved. Therefore, monitoring of WTPs and effective elimination process studies for pharmaceuticals and ECDs are required to control micropollutant contamination of drinking water.

• 한국조경학회지
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• 제34권6호
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• pp.66-77
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• 2007

This study intends to analyze urban peoples' leisure choice attributes of rural areas from a rural demand perspective. For this purpose, this study investigates regional attributes considered in decision making processes for rural tourism destinations of urban residents using a conjoint model as a stated preference model. Based on literature reviews, two questionnaire surveys were conducted. The first questionnaire survey was performed in 4 cities including Seoul, Daejeon, Suwon and Chuncheon with 408 urban residents. The Second questionnaire survey was performed in 5 cities including Seoul, Chuncheon, Daejeon, Jeonju and Busan with about 1,060 urban residents. The study results suggest that the most important attribute in selecting rural ares for tourism are activity programs and convenience of facility, according to part-worth and vector model. The fitness level of model is 0.986, which is very significant. Among the 5 attribute's levels, the rural residents' kindness, the traditional and the ecological programs and the facilities about sign and lodging are more critical factors than other levels. Utilities of each levels decreases as cost and arrival time increases. Regarding the result of market segmentations, respondents having intention to visit can be divided into 4 group; (1) facility or program oriented type, (2) resident's kindness oriented type, (3) arrival time oriented type, and (4) negatively participant type. The results of leisure choice attributes can provide insightful information for regional planning strategies, such as selection of the type of market segments and the key factor of facility and space planning.

• Journal of Korean Biological Nursing Science
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• 제22권3호
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• pp.213-221
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• 2020

Purpose: The purpose of this study was to explore the role performance and related factors of clinical research nurses (CRN) in the process of clinical trials of new drug development. Methods: Study participants were CRN whose affiliation was in non-capital areas and who had been working longer than three months at the time of the data collection. The data collection was conducted with a semi-structured questionnaire development by Hwang & Go, 2011 comprising 16 items of participants' characteristics and 60 items of role performance via in-person and online. Additionally, opinions which would be necessary to establish the CRN role were questioned for the future. A total of 141 questionnaires of 151 questionnaires responses were analyzed using descriptive statistics, t-test, and ANOVA for data analysis as appropriate. Results: The level of overall role performance was above average (5.61±0.90) with the highest in subject care (6.04 ± 0.96) and management and the lowest in self-development (4.39 ± 1.52). The only factor affecting overall role performance was employment status and the only sub-category affected by factors of employment status, and some general characteristics was self-development. Conclusion: From study results, it can be concluded that the CRN seem to perform their proper role. Nonetheless, self-development should be considered as critical aspect for better CRN competency, which is an important aspect toward improving the CRN role performance. Additionally, efforts to improve the level of role performance must be established through stable employment and concrete departmental placement as suggested in this study.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제21권4호
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• pp.401-406
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• 1999

편측성 혹은 양측성 구순구개열환자에서 일차 구순 성형술후 나타나는 이차 구순 기형은 세심한 관찰을 통한 문제점에 대한 정확한 인식을 통해 상순, 홍순부, 피부와 근육등의 적절한 교정술로 심미성, 기능적 회복을 이룰 수 있다. 저자들은 이차 구순 기형으로 나타난 구륜근의 부정유합, 휘파람 변형과 부족한 입술등에 대해 적절한 성형술로 심미적, 기능적으로 양호한 결과를 얻었기에 보고하는 바이다.

• Journal of Species Research
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• 제10권3호
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• pp.246-254
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• 2021

Subalpine and alpine ecosystems are especially vulnerable to temperature increases. Betula ermanii Cham. (Betulaceae) is a dominant broad-leaved tree species in the subalpine zone and is designated as a 'Climate-sensitive Biological Indicator Species' in South Korea. This study aimed to predict the potential distribution of B. ermanii under current and future climate conditions in South Korea using the MaxEnt model. The species distribution models showed an excellent fit (AUC=0.99). Among the climatic variables, the most critical factors shaping B. ermanii distribution were identified as the maximum temperature of warmest month (Bio5; 64.8%) and annual mean temperature (Bio1; 20.3%). Current potential habitats were predicted in the Baekdudaegan mountain range and Mt. Hallasan, and the area of suitable habitat was 1531.52 km², covering 1.57% of the Korean Peninsula. With global warming, future climate scenarios have predicted a decrease in the suitable habitats for B. ermanii. Under RCP8.5-2070s, in particular, habitat with high potential was predicted only in several small areas in Gangwon-do, and the total area suitable for the species decreased by up to 97.3% compared to the current range. We conclude that the dominant factor affecting the distribution of B. ermanii is temperature and that future temperature rises will increase the vulnerability of this species.

• 해양환경안전학회지
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• 제21권1호
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• pp.83-90
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• 2015

육지와 도서를 연결하는 차도선은 교통수단 기능과 물자수송을 담당하는 중요한 역할을 하고 있다. 이에 따라 차도선의 효율성 증대와 안전성 향상 및 경제적 요건 등을 고려한 선박이 개발 중이다. 유럽에서는 승객의 편의성 및 경제성을 고려하여 양방향 차도선(Double-ended Car Ferry)의 개발이 활발히 진행 중이며 국내에서도 이에 대한 연구 개발이 필요하다. 본 연구에서는 유한요소법을 이용하여 차도선 제작 시 축계의 제작 및 강도의 문제를 Campbell Diagram 및 Modal 해석을 통한 동특성 분석을 하였다. 이를 통하여 양방향 차도선의 추진 방향 및 후진 방향 축계 작동 시 공진 현상 및 위험속도를 분석 결과 안정성을 보인 것으로 판단된다.

• IMMUNE NETWORK
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• 제5권2호
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• pp.110-116
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• 2005

Background: As an attempt to develop a strategy to improve the protective immune response to GM-CSF-secreting CT26 (GM-CSF/CT26) tumor vaccine, we have investigated whether the apoptogenic treatment of GM-CSF/CT26 prior to vaccination enhances the induction of anti-tumor immune response in mouse model. Methods: A carcinogeninduced mouse colorectal tumor, CT26 was transfected with GM-CSF gene using a retroviral vector to generate GM-CSF-secreting CT26 (CT26/GM-CSF). The CT26/GM-CSF was treated with ${\gamma}$-irradiation or mitomycin C to induce apoptosis and vaccinated into BALB/c mice. After 7 days, the mice were injected with a lethal dose of challenge live CT26 cells to examine the protective effect of tumor vaccination in vivo. Results: Although both apoptotic and necrotic CT26/GM-CSF vaccines were able to enhance anti-tumor immune response, apoptotic CT26/GM-CSF induced by pretreatment with ${\gamma}$-irradiation (50,000 rads) was the most potent in generating the anti-tumor immunity, and thus 100% of mice vaccinated with the apoptotic cells remained tumor free for more than 60 days after tumor challenge. Conclusion: Apoptogenic pretreatment of GM-CSF-secreting CT26 tumor vaccine by ${\gamma}$-irradiation (50,000 rads) resulted in a significant enhancement in inducing the protective anti-tumor immunity. A rapid induction of apoptosis of CT26/GM-CSF tumor vaccine at the vaccine site might be critical for the enhancement in anti-tumor immune response to tumor vaccine.

• 동의생리병리학회지
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• 제24권5호
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• pp.831-836
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• 2010

In addition to spice, black pepper (Piper nigrum Linne : PnL) has been used as herbal medicine because of its function in anti-oxidation, anti-inflammation, and anti-carcinogenesis. Recently, it has been reported that piperine, a component of PnL, inhibits adipocyte differentiation by repressing various adipogenic gene expressions. In this study, we determined whether piperine is a major constituent of PnL that confers the anti-adipogenic activity at whole genome level. Differentiation of 3T3-L1 pre-adipocytes was induced in presence of PnL extract or piperine. To compare genes that are regulated by PnL extract or piperine, we performed expression profiling using microarrays (Agilent Mouse 44k 4plex). RNA samples were labeled with Cy3 and Cy5, respectively. Labeled samples were hybridized to the microarrays. Results were filtered and cut off set p<0.05. Genes exhibiting significant differences in expression level were classified into Gene Ontology (GO)-based functional categories (http://www.geneontology.org) and KEGG (http://www.genome.jp/kegg/). Extract of PnL and its component piperine reduced lipid accumulation in 3T3-L1 cells during adipogenesis. Such anti-adipogenic activity appears to result from down-regulation of transcription factor genes involved in adipogenesis, and other genes involved in fatty acid synthesis, transport, triglyceride synthesis, and carbohydrate metabolism. These genome-wide studies lead to conclude that piperine, as a critical component of PnL, plays common role with PnL in anti-adipogenesis.

• 대한기계학회논문집A
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• 제39권10호
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• pp.1011-1019
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• 2015

Insulated gate bipolar transistor (IGBT) 소자는 전동차, 항공기 및 전기 자동차에 가장 많이 사용되는 고전압, 고전력용 전력 반도체이다. 그러나 IGBT 전력소자는 동작 시 발열 온도가 매우 높고, 이로 인해, IGBT 소자의 신뢰성 및 성능에 큰 영향을 미치고 있다. 따라서 발열 문제를 해결하기 위한 IGBT 모듈 패키지의 방열 설계는 매우 핵심적인 기술이며, 특히, 소자가 동작 한계 온도에 올라가지 않도록 방열 설계를 적절히 수행하여야 한다. 본 논문에서는 전동차에 사용되는 1200 A, 3.3 kV 급 IGBT 모듈 패키지의 열 특성에 대해 수치해석을 이용하여 분석하였다. IGBT 모듈 패키지에 사용되는 다양한 재료 및 소재의 두께에 대한 영향을 분석하였으며, 실험계획법을 이용한 최적화 설계를 수행하였다. 이를 통하여 열 저항을 최소화하기 위한 최적의 방열 설계 가이드 라인을 제시하고자 하였다.