• 제목/요약/키워드: CopA3

검색결과 447건 처리시간 0.026초

9-Meric Peptide Analogs of Defensin-like Antimicrobial Peptide Coprisin with Potent Antibacterial Activities with Bacterial Sell Selectivites

  • Shin, Areum;Lee, Eunjung;Kim, Jin-Kyoung;Bang, Jeong-Kyu;Kim, Yangmee
    • Bulletin of the Korean Chemical Society
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    • 제35권9호
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    • pp.2809-2812
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    • 2014
  • The 43-residue defensin-like peptide coprisin, which is isolated from dung bettle, Copris tripartitus, is a potent antimicrobial peptide. In our previous work, we determined the tertiary structure of coprisin and found that alpha helical region of coprisin from residue 19 to residue 30 is important for its antimicrobial activities. Here, we designed cop12mer and cop9mer analogs of coprisin based on the tertiary structure of coprisin. To investigate the relationship between hydrophobicity and antimicrobial activities and develop the potent peptide antibiotics, we designed cop9mer-1 with substitution of $His^2$ with Trp in cop9mer. The results showed that cop9mer-1 has higher toxicities as well as improved antimicrobial activities compared to cop9mer. In order to reduce the toxicity of cop9mer-1, we designed cop9mer-2 and cop9mer-3 with substitution of $Cys^3$ with Lys or Ser. Substitution of $Cys^3$ with these hydrophilic amino acids results in lower cytotoxicities compared to cop9mer-1. Cop9mer-2 with substitution of $Cys^3$ with Lys in Cop9mer-1 showed high antibacterial activities against drug resistant bacteria without cytotoxicity. Antibiotic action of cop9mer-1 analog appears to involve permeabilization of the bacterial cell membrane while cop9mer-2 and cop9mer-3 may have different mechanism of action. These results imply that that optimum balance in hydrophobicity and hydrophilicity in these 9-meric peptides plays key roles in their antimicrobial activities as well as cytotoxicities.

Dynamic Profile of the Copper Chaperone CopP from Helicobacter Pylori Depending on the Bound Metals

  • Hyun, Ja-shil;Park, Sung Jean
    • 한국자기공명학회논문지
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    • 제20권3호
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    • pp.76-81
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    • 2016
  • Copper is an elemental ion in living organisms. CopP from Helicobacter Pylori (HpCopP) is a copper(I)-binding protein and was suggested as regulator of copper metabolism in vivo. Previously, the metal binding property of HpCopP for Ag(I), Cu(I), and Cu(II) as well as the tertiary structure of HpCopP was shown. In this study, the dynamic profiles of HpCopP depending on metal binding were studied using ${^1H}-^{15}N$ steady-state NOE analysis. The heteroNOE experiment was performed for apo-CopP or metal-bound CopP. The obtained NOE values were analyzed and compared to figure out the effect of metals on the structural flexibility of HpCopP. As a result, Ag(I) and Cu(I) ions improved the rigidity of the structure while Cu(II) ion increased the flexibility of the structure, suggesting the oxidation of the CXXC motif decreases the structural stability of HpCopP.

Effects of the Synthetic Coprisin Analog Peptide, CopA3 in Pathogenic Microorganisms and Mammalian Cancer Cells

  • Kim, In-Woo;Kim, Soon-Ja;Kwon, Yong-Nam;Yun, Eun-Young;Ahn, Mi-Young;Kang, Dong-Chul;Hwang, Jae-Sam
    • Journal of Microbiology and Biotechnology
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    • 제22권1호
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    • pp.156-158
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    • 2012
  • A synthetic coprisin analog peptide, 9-mer dimer CopA3 (CopA3) was designed based on a defensin-like peptide, Coprisin, isolated from the bacteria-immunized dung beetle Copris tripartitus. Here, CopA3 was investigated for its antimicrobial activity and cancer cell growth inhibition. CopA3 showed antimicrobial activities against various pathogenic bacteria and yeast fungus with MIC values in 2~32 ${\mu}M$ ranges, and inhibited the cell viabilities of pancreatic and hepatocellular cancer cells, except MIA-Paca2, Hep3B, and HepG2 cells, in a dose-dependent manner. The average $IC_{50}$ values of CopA3 against pancreatic and hepatocellular cancer cells were 61.7 ${\mu}M$ and 67.8 ${\mu}M$, respectively. The results indicate that CopA3 has potential in the treatments of pancreatic and hepatocellular cancers as well as microorganism infection disease.

전사 수준에서 repABC 유전자 발현을 조절하는 CopA 단백질의 역할 (Role of CopA to Regulate repABC Gene Expression on the Transcriptional Level)

  • 김삼웅;갈상완;지원재;방우영;김태완;백인규;방규호
    • 생명과학회지
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    • 제34권2호
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    • pp.86-93
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    • 2024
  • 플라스미드의 복제는 엄격하게 조절되어야 하기 때문에 일반적으로 rolling circle 복제를 수행하는 플라스미드들은 복제 개시인자인 RepB는 전사 및 번역 수준에서 엄격하게 조절되어 일정한 copy number를 유지한다. 플라스미드 pJB01에는 단일 오페론으로 구성된 세 개의 orfs (copA, repB, repC 또는 repABC)가 포함되어 있다. 아미노산 서열 분석에서 pJB01 CopA는 다른 플라스미드의 복제 수 조절 단백질로서 Cops와 상동성을 보였다. pMV158의 CopG와 비교할 때, CopA는 플라스미드의 일반화된 억제자의 모티브로 알려진 RHH (ribbon-helix-helix)를 형성하는 것으로 추정된다. gel mobility shift assay 결과 정제된 융합 단백질이 repABC 오페론의 operator 영역에 결합하는 것으로 나타났다. 전사 수준에 대한 CopA의 기능적 역할을 조사하기 위해 CopA R16M, K26R 및 E50V와 같은 세 개의 포인트 돌연변이가 CopA의 코딩 프레임에서 구성되었다. CopA R16M, K26R 및 E50V 돌연변이의 repABC mRNA 수준은 CopA wt보다 각각 1.84, 1.78 및 2.86배 증가했다. 또한 세 개의 CopA 유전자의 돌연변이로 인한 복제 수도 CopA wt보다 각각 1.86, 1.68 및 2.89배 증가했다. 이러한 결과는 CopA가 전사 억제자이며 복제 개시자로서 repABC mRNA 및 RepB 단백질 수를 감소시킴으로써 pJB01의 복제 수를 감소시키는 것으로 제의된다.

Y-Balance Test 시 지지발이 활동발에 미치는 영향 (Effects of support feet on active feet during Y-Balance Test)

  • 우병훈
    • 한국응용과학기술학회지
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    • 제40권6호
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    • pp.1249-1258
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    • 2023
  • 본 연구의 목적은 YBT(Y-balance test) 시 지지발의 COP 변인이 활동발의 상대도달거리와 종합점수에 미치는 영향을 밝히는 것이다. 연구의 대상자로 20대 성인 남성 26명(연령: 24.4±3.0 년, 신장: 171.0±10.5 cm, 체중: 72.1±12.1 kg 다리길이: 88.2±5.8 cm)이 연구에 참여하였다. 연구의 측정도구인 YBT를 통하여 활동발의 상대도달거리, 종합점수, 지지발의 COP 변인들을 좌우발에서 측정하여 결과를 도출하였다. YBT 시 지지발의 COP 변인이 상대도달거리와 종합점수가 미치는 영향을 알아보기 위하여 다중회귀분석을 이용하였다. 이에 따른 연구결과로, YBT 동안 오른발 지지 시 후외측 방향에서 전후, 좌우 COP속도, COP속도의 영향이 크고, 왼발 지지 시 후내측 방향에서 전후, 좌우 COP속도, COP속도의 영향으로 상대도달거리 및 종합점수를 향상시켰다.

Characterization of CaCOP1 Gene in Capsicum annuum Treated with Pathogen Infection and Various Abiotic Stresses

  • Guo, Jia;Seong, Eun-Soo;Wang, Myeong-Hyeon
    • Journal of Applied Biological Chemistry
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    • 제50권4호
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    • pp.227-233
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    • 2007
  • We characterized a full-length cDNA of CaCOP1 from pepper. Phylogenetic analysis based on the deduced amino acid sequence of CaCOP1 cDNA revealed high sequence similarity to the COP1 gene in Oryza sativa (84% identity). CaCOP1 shares high sequence identity with regulatory protein in Arabidopsis (84%), constitutively photomorphogenic 1 protein in Pisum sativum (81%) and COP1 homolog in Lycopersicon esculentum (79%). CaCOP1 gene exists single copy in the chili pepper genome. Expression of CaCOP1 was reduced in response to inoculation of non-host pathogens. The expression of this gene under abiotic and oxidative stresses was investigated, including 200 mM NaCl, 200 mM mannitol, cold ($4^{\circ}C$), 100 ${\mu}M$ abscisic acid (ABA), and 10 mM hydrogen peroxide ($H_2O_2$). CaCOP1 was induced significantly 3 h after low temperature treatment but not by dehydration or high salinity. Moreover, CaCOP1 was not induced by plant hormone ABA. These observations suggest that CaCOP1 gene plays a role in abiotic stress and may be belong to ABA-independent regulation system.

보행 시 부하 위치에 따른 족저압 중심(COP) 이동 분석 (The Analysis of Center of Pressure(COP) Displacement under Loading Position during Walking)

  • 박수진;김진상
    • 대한물리의학회지
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    • 제5권1호
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    • pp.15-24
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    • 2010
  • Purpose : The purpose of this study was to investigate the effect of loading position on plantar center of pressure(COP) displacement when carrying a schoolbag during walking. Methods : Forty-four normal subjects were randomly assigned to five groups according to the method of carrying a schoolbag. The carrying a schoolbag methods were classified into five conditions: no bag (condition 1), a backpack (condition 2), a shoulder bag (condition 3), a cross bag (condition 4), a one-hand bag (condition 5). COP displacement such as anteroposterior distance and mediolateral distance of COP were measured with F-scan system. The repeated one-way analysis of variance (ANOVA) and independent t-test were used to confirm the statistical significance. Results : In the comparison of parameters of COP displacement between conditions, anteroposterior distance and mediolateral distance in the left foot and mediolateral distance in the right foot were not significantly different(p>.05), but anteroposterior diatance in the right foot was significantly different(p>.05). Between left and right foot, at condition 1 and 5 the mediolateral distance of COP was significantly different(p<.05) but anteroposterior distance at condition 1 and 5, anteroposterior distance and mediolateral distance of COP at condition 2, 3 and 4 were not significantly different(p>.05). Conclusion : These findings showed that the various loading position by five types of carrying a schoolbag didn't have influence significantly on COP displacement on during walking because of mechanism of postural adaption.

건축물 용도별 내부 발열부하에 따른 수열원, 지열원 및 하이브리드 히트펌프 시스템의 에너지 성능 비교 분석 (Comparative Analysis of Energy Performance of Hydrothermal, Geothermal Source and Hybrid Heat Pump System According to Internal Heat Load for Office, School and Smart Farm)

  • 박시훈;민준기
    • 한국지열·수열에너지학회논문집
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    • 제18권3호
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    • pp.19-30
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    • 2022
  • In this study, comparative analysis of energy performance in Taebaek city, a test area, by applying hydrothermal, geothermal source and hybrid heat pump system to office, school and smart farms with different internal heat loads. The conclusion is as follows. In the load characteristics by use of buildings, it was found that office had a large cooling load compared to heating load, school had a large heating load compared to cooling load, and smart farm had only cooling load year-round. Performance analysis of the heat pump system in office shows that the cooling COP of the hydrothermal source is 5.12% and the heating COP is 3.22% lower based on the geothermal source, the cooling COP of the hybrid is 0.41% higher, and the heating COP is the difference in performance appeared sparsely. The performance analysis of the heat pump system in school showed that the cooling COP of the hydrothermal source was 10.44% and the heating COP 3.22% lower based on the geothermal source, and the performance difference between the hybrid cooling and heating COP was insignificant. Heat pump system performance analysis in smart farm only occurred with cooling load. Based on geothermal sources, the cooling COP of the hydrothermal source was 46% and the cooling COP of the hybrid was 19.65%, respectively.

지열히트펌프의 작동시간 경과에 따른 COP 변화에 대한 연구 (Study on COP Variations with the duration of Ground Source Heat Pump Systems Operation)

  • 이용규;백남춘;윤응상
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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    • pp.198.2-198.2
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    • 2010
  • In this study, the COP variation with the duration of Ground Source Heat Pump (GSHP) systems operation was analyzed by experiment. This experimental facility was installed in residential house as a back-up device of solar thermal heating system. The capacity of heat pump is 2.5 kW with a vertical bore hole of 150m depth. The COP of GSHP is varied, depending on the ground temperature which is used as a heat source. The ground heat source temperature influencing heating COP is the soil or rock temperature which adjoin with geo-source heat exchanger. This temperature is decreased rapidly according to the operation duration of heat pump. As a result, COP of GSHP is decreased to 3 in one hour of continuous operation time.

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열원 및 부하조건에 따른 물-공기 히트펌프 시스템의 성능분석 (Performance Analysis of Water-to-Air Heat Pump System under Water Temperature and Load Ratio)

  • 조용;이동근
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2011년도 춘계학술대회 초록집
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    • pp.194.2-194.2
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    • 2011
  • Heating and cooling performance has been analyzed for the water-source heat pump system using raw water from Daechung reservoir. During heating operation from March to May, water temperature is not good condition for a heat source due to the higher atmospheric temperature. Avearged heating load ratio is only 14.3%, and the averaged unit COP and system COP are estimated to be 2.46 and 2.15 respectively. The COP is affected considerably by the water temperature, and the unit COP is increased from 2.16 at $5^{\circ}C$ to 2.95 at $11^{\circ}C$. Cooling performance is analyzed with the measured data from June to August. During cooling operation, raw water has lower temperature by 4. $5^{\circ}C{\sim}4.7^{\circ}C$ than the atmosphere. The load ratio is 39.2%, and the averaged unit COP and system COP are estimated to be 7.25 and 6.13 respectively. The heating COP is affected by the load ratio rather than water temperature. The COP is increased for 20%~40% load ratio, while is decreased for 40%~60% load ratio. It is estimated that the compressor operation combination for 3 (two constant speed and one inverter) compressors is changed for the load ratio.

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