• Korean Journal of Clinical Laboratory Science
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• v.55 no.3
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• pp.184-194
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• 2023

In this study, we investigated whether p-anisaldehyde (PAA), the main component of essential oils derived from anise seeds, influences the differentiation of mouse C2C12 myoblasts. Cells were induced to differentiate over 5 days using a differentiation medium with or without PAA (50 or 200 mg/mL). Myotube length and diameter were measured, and the expressions of myogenic markers (myoblast determination protein 1, myogenin, myocyte enhancer factor 2, muscle creatine kinase, and myosin heavy chain) and atrophy-related genes (atrogin-1 and muscle ring finger-1 [MuRF-1]) were assessed by quantitative real-time polymerase chain reaction. Additionally, protein kinase B (Akt) phosphorylation was monitored by western blotting. PAA significantly induced the formation of smaller and thinner myotubes and reduced myogenic marker expression. Furthermore, PAA increased the expressions of atrogin-1 and MuRF-1 and simultaneously reduced Akt phosphorylation. Our findings indicate that PAA inhibits the myogenic differentiation of C2C12 cells by reducing the phosphorylation and activation of Akt.

• Journal of Families and Better Life
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• v.6 no.1
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• pp.189-203
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• 1988

The main objective of this dissertation is to find out the measurement methods of household work organization and manger style and the quantitative analysis methods of influential factors on household work organization and manager style. Subjects were homemakers living in metropolitan , medium and small cities. Questionnaires were sent to subjects and usable responses were 1139 out of 1239 received questionnaires. Data collected through both preliminary and main survey were analyzed by using frequencies, percentage, analysis of variance, chi-square test and pearson's correlation analysis. Results are as follows; 1) The degree of household work organization was distributed on upper middle level exceeding middle score. Among subsystems of household work such as task assignment , test regularization, task arrangement and task standardization. subjects show the highest positive attitude in task standardization. 2) The characteristics of management in household work have task centered orientation. By the prority of task centered orientation, the order was integrated style. dedicated style. It means that the most ideal style is an integrated style which accounts for one-third of the total homemakers. 3) Household organization's subsystems and household work manager style are related with socio-demographic variables. Integrated style is significantly related with homemaker's employment and having helpers for household work. homemakers who are employed and have helpers for household work exhibit high scores in task assignment and task arrangement. Dedicated style is significantly related with homemaker's age and the number of children. Homemakers who are older and have many children exhibit high scores in task assignment. Related style is significantly related with marriage duration, education level and income level. Homemakers who have a long marriage duration exhibit high scores in task assignment and who have high education and income level exhibit high scores in task regularization. Seperates style is significantly related with homemaker's age m marriage duration an family life cycle. homemakers who ar older, have a long marriage duration and are of late stage in family life cycle exhibit seperated style and high scores in organization. To summarize research findings, household work manger and household work organization are related with family life experiences, and task assignment is an influential variable on manager style. in conclusion, it is evident that household work organization emphasizes efficiency as cognitive component and such organization. Finally it is necessary to broaden this research considered social psychological variables. And also it needs to build household manger style models appropriate fro the characteristics of each style.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.3
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• pp.367-373
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• 2014

In this study, we investigated the anticancer activity of methyl gallate (MG), which is the major biologically active component of Galla Rhois, in RC-58T/h/SA#4 human prostate cancer cells. MG inhibited cell proliferation in a dose-dependent manner. Cell death induced by MG increased the population of cells in sub-G1 phase, formation of apoptotic bodies, nuclear condensation, and DNA fragmentation. Apoptosis induced by MG was associated with activation of initiator caspases-8 and -9 as well as effector caspase-3. Endocrine disruptors such as dioxin and bisphenol A increased growth of RC-58T/h/SA#4 cells in charcoal-treated FBS (cFBS) medium. Cell proliferation was highest upon treatment with 1 nM and $0.1{\mu}M$ dioxin and bisphenol A, respectively. MG also dose-dependently inhibited cell proliferation in RC-58T/h/SA#4 cells treated with endocrine disruptors. These results indicate that MG exerts anticancer effects on RC-58T/h/SA#4 primary human prostate cancer cells.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.1
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• pp.1-17
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• 2020

Three-dimensional microscopic approaches in histopathology display multiplex properties that present puzzling questions for specimens as related to their comprehensive volumetric information. This information includes spatial distribution of molecules, three-dimensional co-localization, structural formation and whole data set that cannot be determined by two-dimensional section slides due to the inevitable loss of spatial information. Advancement of optical instruments such as two-photon microscopy and high performance objectives with motorized correction collars have narrowed the gap between optical theories and the actual reality of deep tissue imaging. However, the benefits gained by a prolonged working distance, two-photon laser and optimized beam alignment are inevitably diminished because of the light scattering phenomenon that is deeply related to the refractive index mismatch between each cellular component and the surrounding medium. From the first approaches with simple crude refractive index matching techniques to the recent cutting-edge integrated tissue clearing methods, an achievement of transparency without morphological denaturation and eradication of natural and fixation-induced nonspecific autofluorescence out of real signal are key factors to determine the perfection of tissue clearing and the immunofluorescent staining for high contrast images. When performing integrated laboratory workflow of tissue for processing frozen and formalin-fixed tissues, clear lipid-exchanged acrylamide-hybridized rigid imaging/immunostaining/in situ hybridization-compatible tissue hydrogel (CLARITY), an equipment-based tissue clearing method, is compatible with routine procedures in a histopathology laboratory.

• Journal of Life Science
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• v.21 no.1
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• pp.68-73
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• 2011

$\beta$-1,3-glucanase from Pyrococcus furiosus was applied for the saccharification of laminarin, which is a major oligo-saccharide component of brown algae, and the reaction mixture produced from laminarin was utilized as a substrate for alcohol fermentation using yeast. To prepare the recombinant $\beta$-1,3-glucanase, a $\beta$-1,3-glucanase gene was overexpressed in Escherichia coli and purified. Laminarin was degraded to an oligo- and mono-saccharide, such as glucose, after reaction with the purified recombinant $\beta$-1,3-glucanase, and the products after enzymatic treatment were confirmed by TLC and HPLC analysis. Decomposed laminarin after enzyme reaction was only added to the medium as a C-source for yeast alcohol production reaction. 0.3% alcohol production was detected from the cultured broth by gas chromatography after 48 hr of incubation. Further evaluation for optimal conditions of saccharification and alcohol fermentation can be suggested, as well as the possibility of using this enzymatic method to produce ethanol using laminarin.

• Journal of Korea Entertainment Industry Association
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• v.14 no.8
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• pp.141-152
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• 2020

This thesis is a study on the creation of characters of "personality actors and character actors" among the concepts of "star persona" of S. Prince. This study selected 7 representative films of Jung Woo-sung, a representative personality actor in the Korean film industry, and analyzed how 'Jung Woo-sung-character' is embodied in the film as a character component suggested by R. Dyer. In the traditional sense, the actor's acting has been highly valued for transformation and impersonation in character creation, but discussing the method of creating a character focusing only on the actor's acting in a movie acting implemented in combination with advanced technology I don't think it's suitable for movie acting research. Theorists such as R.Dyer and Edgar Morin, who studied film actors and stars, also emphasize that 'movie actors are different from theater actors' (by the film medium). Therefore, in this thesis, a detailed analysis of the acting of a personality actor was avoided. As Morin pointed out that the character in the movie can be implemented through the actor's image and type, not playing the type of role. I tried to propose the significance of this character creation method of the personality actor by analyzing actor Jung Woo-sung and his character.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.358-364
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• 2006

This study was conducted to develop optimal solid culture medium for Tricholoma matsutake. As the solid matrix, granitic soil, perlite, vermiculate, pine sawdust and peat moss were compared regarding their effected on mycelial growth. Ergosterol content which is a fungal wall component was used as the growth index of the mycelia. Among the various solid matrixes, the granitic soil, perlite and mixture of the two supported the growth most. Barely flour appeared to be very effective on the stimulating of the mycelial growth when added to the solid matrix. An mixture of the matrix contained an even (1:1:1:1, v/v/v/v) mixture of granitic soil, perlite, vermiculate and pine sawdust. T. matsutake started growth 2 weeks after inoculation and reached stationary growth phase after 8th weeks in the solid matrix mixture. The mycelial density in the solid matrix was 7 times higher than that in fairy-ring soil. In addition, 30~70% water content and 10% humus soil in the solid matrix also supported good growth suggesting that T. matsutake needs humus soil for a nutrient sources. The solid matrix developed in the present study could be used to study physiological characteristics of T. matsutake as well.

• Journal of Life Science
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• v.29 no.10
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• pp.1120-1125
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• 2019

The short-lived nematode Caenorhabditis elegans has been used as a model organism for many studies, including lifespan extension. To screen common seaweeds for natural anti-aging agents, the lifespan of C. elegans (N2 wild-type strain) was measured by its hatch rate, growth rate, survival rate, chemotaxis, brood size, and egg-laying time after exposure to nematode growth medium (NGM) containing seaweed extracts. Approximately 30 animals synchronized at the first larval stage were incubated until they reached their adult stages before laying their eggs and were transferred to fresh NGM every 3 days. We also identified the major active compound from the seaweed by gas chromatography-mass spectrometry and tested its optimal dose for longevity. Of 13 common seaweed species, an ethanol extract of the brown seaweed Hizikia fusiformis showed the greatest effect on hatching, growth, and survival rates. The lifespan of C. elegans was significantly expanded 1.54-fold and 1.23-fold in the presence of the ethanol extract (0.05 mg/ml) and the main active component, fucosterol (0.05 mg/ml), respectively. Exposure to the ethanol extract also increased chemotaxis 1.13-fold, decreased brood size 0.74-fold, and shortened egg-laying time 0.96-fold. These results suggest that the aquaculturable H. fusiformis may be a promising source of a diet supplement to support health care.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.18 no.2
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• pp.113-126
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• 2023

In this study, a total of two hypotheses were established and tested to find out how entrepreneurship in manufacturing SMEs affects organizational effectiveness through technological innovation. The key results of this paper are as follows. First, it was confirmed that innovativeness, a component of entrepreneurship, did not affect organizational commitment, but it did affect job satisfaction. On the other hand, it was confirmed that proactiveness and risk-taking did not affect both job satisfaction and organizational commitment. Second, process innovation played a mediating role in the relationship between innovativeness and job satisfaction, proactiveness and job satisfaction, and proactiveness and organizational commitment. However, it was confirmed that product innovation did not play a mediating role at all in the relationship between the components of entrepreneurship and the components of organizational effectiveness. This study provided academic and practical implications by identifying the antecedent factors that affect the organizational effectiveness of manufacturing SMEs. From an academic point of view, previous studies did not differentiate by industry or mainly selected and studied sample subjects from industries such as IT, hotel, service, and tourism. However, this study investigated how entrepreneurship affects organizational effectiveness through technological innovation targeting manufacturing SMEs, and provides the research results. In addition, from a practical point of view, manufacturing SMEs need to make efforts to improve workers' proactiveness, innovativeness, and process innovation capabilities in order to improve workers' job satisfaction and organizational commitment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.2
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• pp.190-194
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• 2008

Physicochemical properties of the green tea component enriched mushroom (Flammulina velutipes) were investigated. The yield of mushroom was highest with green tea powder untreated sample and the yield was decreased by the addition of green tea powder. However, hardness was increased by the addition of green tea powder. Analysis of catechins and caffeine in mushrooms showed that catechins were not transferred into mushrooms, whereas caffeine content was increased. The content of total polyphenol in mushroom was not affected by the addition of green tea powder and crude catechins. Analyses of aroma patterns using the electronic nose based on GC with SAW sensor showed that new peaks occurred from 3 to 6 sec in green tea added mushroom. This study showed that functional components and quality of mushroom were possibly improved by incorporating green tea powder in growth medium.