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Culture of Tricholoma matsutake Mycelium using Solid Matrix  

Lee, Wi Young (Div. of Biotechnology, Korea Forest Research Institute)
Ahn, Jin Kwon (Div. of Biotechnology, Korea Forest Research Institute)
Ka, Kang Hyeon (Div. of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Park, Hyun (Div. of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.95, no.3, 2006 , pp. 358-364 More about this Journal
Abstract
This study was conducted to develop optimal solid culture medium for Tricholoma matsutake. As the solid matrix, granitic soil, perlite, vermiculate, pine sawdust and peat moss were compared regarding their effected on mycelial growth. Ergosterol content which is a fungal wall component was used as the growth index of the mycelia. Among the various solid matrixes, the granitic soil, perlite and mixture of the two supported the growth most. Barely flour appeared to be very effective on the stimulating of the mycelial growth when added to the solid matrix. An mixture of the matrix contained an even (1:1:1:1, v/v/v/v) mixture of granitic soil, perlite, vermiculate and pine sawdust. T. matsutake started growth 2 weeks after inoculation and reached stationary growth phase after 8th weeks in the solid matrix mixture. The mycelial density in the solid matrix was 7 times higher than that in fairy-ring soil. In addition, 30~70% water content and 10% humus soil in the solid matrix also supported good growth suggesting that T. matsutake needs humus soil for a nutrient sources. The solid matrix developed in the present study could be used to study physiological characteristics of T. matsutake as well.
Keywords
Tricholoma matsutake; solid media; ergosterol; mycelial culture; humus soil;
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Times Cited By KSCI : 2  (Citation Analysis)
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