• 제목/요약/키워드: Cell Observation

검색결과 913건 처리시간 0.024초

Serum Tumor Markers, Hypoxia-Inducible factor-1α HIF-1α and Vascular Endothelial Growth Factor, in Patients with Non-small Cell Lung Cancer Before and after Intervention

  • Liang, Jun;Qian, Ying;Xu, Dan;Yin, Qun;Pan, Hui-Juan
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권6호
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    • pp.3851-3854
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    • 2013
  • Objective: To explore changes in the serum tumor makers, hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) and vascular endothelial growth factor (VEGF) level and their relations in patients with non-small cell lung cancer (NSCLC) before and after intervention. Materials and Methods: Forty patients with NSCLC and 40 healthy individuals undergoing physical examination in our hospital provided the observation and control groups. HIF-$1{\alpha}$ and VEGF levels in serum were detected by enzyme-linked immuno-sorbent assay (ELISA) in the observation group before and after intervention and in control group on the day of physical examination, along with serum carcino-embryonic antigen (CEA), neuron-speci ic enolase (NSE) and squamous cell carcinoma antigen (SCC) levels in the observation group with a fully automatic biochemical analyzer. Clinical effects and improvement of life quality in the observation group were also evaluated. Results: The total effective rate and improvement of life quality after treatment in observation group were 30.0% and 32.5%, respectively. Serum HIF-$1{\alpha}$ and VEGF levels in the control group were lower than that in observation group (p<0.01), but remarkably elevatedafter intervention (p<0.01). In addition, serum CEA, NSE and SCC levels were apparently lowered by treatment (p<0.01). Serum HIF-$1{\alpha}$ demonstrated a positive relation with VEGF level (p<0.01) and was inversely related with CEA, NSE and SCC levels (p<0.01). Conclusions: Significant correlations exist between marked increase of serum HIF-$1{\alpha}$ and VEGF levels and decrease of indexes related to hematological tumor markers in NSCLC patients after intervention.

A Study on an Automatic Multi-Focus System for Cell Observation

  • Park, Jaeyoung;Lee, Sangjoon
    • Journal of Information Processing Systems
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    • 제15권1호
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    • pp.47-54
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    • 2019
  • This study is concerned with the mechanism and structure of an optical microscope and an automatic multi-focus algorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cell images quickly, a z-axis actuator with a resolution of $0.1{\mu}m$ was designed to control an optical microscope Moreover, a lighting control system was constructed to select the color and brightness of light that best suit the object being viewed. Cell images are captured by the instrument and the sharpness of each image is determined using Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are applied to automatically find the most vivid points among multiple images of a single object. A cancer cell imaging experiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained using this microscope. The proposed system is expected to save time and effort required to extract suitable cell images and increase the convenience of cell analysis.

Effect of AL072, a Novel Anti-Legionella Antibiotic, on Growth and Cell Morphology of Legionella pneumophila

  • Kang, Byeong-Cheol;Park, Jae-Hak;Lee, Yong-Soon;Suh, Jung-Woo;Chang, Jun-Hwan;Lee, Chul-Hoon
    • Journal of Microbiology and Biotechnology
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    • 제9권3호
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    • pp.371-375
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    • 1999
  • AL072 is a potent anti-Legionella antibiotic produced by Streptomyces strain AL91. The minimum inhibitory concentration (MIC) of AL072 against Legionella pneumophila was 0.2$\mu$g/ml. Bacterial growth was rapidly inhibited at the dose range between the MIC and 20 times of the MIC when the antibiotic was added at the mid-exponential phase. Ultrastructural changes in L. pneumophila were observed upon treatment with AL072. Under electron microscopical observation, the organisms treated with AL072 exhibited characteristic morphological changes in the cellular outer coat. Also irregular morphological changes, such as the formation of filamentous materials in the cytoplasm, an increase in the size and number of cytoplasmic vacuoles, the extruding of cytoplasmic contents, the formation of spheroplast and ghost cells, and blebbings in the cell wall were observed. Furthermore, immunoelectron microscopical observation of the group treated with the MIC showed that the immune complex attached mainly to the cell wall. The results of these experiments indicate that AL072, like the inhibitors of cell wall synthesis, act selectively on the cell wall of L. pneumophila.

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UMTS 단말기 모뎀의 초기 셀 탐색 파라미터의 영향에 대한 연구 (A Study on Initial Cell Search Parameters in UMTS Terminal Modem)

  • 류동렬;김용석;옥광만;최형진
    • 한국통신학회논문지
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    • 제28권5A호
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    • pp.267-275
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    • 2003
  • UMTS는 1) 슬롯 동기 획득, 2)코드그룹 및 프레임 동기 획득, 3) 코드 식별 과정으로 구성된 초기 셀 탐색방법을 사용한다. 단말 모뎀의 초기 셀 포착 성능은 탐색기의 관찰 구간과 임계값 등의 탐색 파라미터에 의하여 크게 영향을 받는다. 본 논문의 목적은 최소 평균 포착 시간을 구하기 위한 단계별 관찰 구간과 임계값을 구하는데 있다. 본 논문에서는 3단계 탐색과정을 상태 다이어그램을 통하여 모델링하고 이로부터 단계별 평균 검출 시간과 전체 평균 포착 시간을 유도한다. 또한 초기 발진기 오차를 고려한 셀 탐색 알고리즘을 제시하고, 모의 실험결과와 유도한 단계별 평균 검출 시간을 이용하여 적절한 각 단계별 검출 시간을 설정한다. 각 단계의 최적 임계값은 유도된 전체 평균 포착 시간으로부터 결정될 수 있으며, 결정된 파라미터를 이용하여 다중경로 페이딩 채널에서 500ms이내의 성능을 얻을 수 있었다.

BOES 데이터를 이용한 정밀 시선 속도 추정 프로그램 RVI2CELL 개발 (DEVELOPMENT OF RVI2CELL - A PRECISE RADIAL VELOCITY ESTIMATION PROGRAM WITH BOES DATA)

  • 한인우;김강민;이병철
    • 천문학논총
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    • 제22권3호
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    • pp.75-81
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    • 2007
  • In this study we present basic principles and features of RVI2CELL, a precise RV (radial velocity) estimation program to process stellar spectra obtained through iodine cell. RVI2CELL is very robust and fast program. The instrument profile can be modeled as a sum of Gaussian functions or a non-parametric arbitrary shape. The RV accuracy estimated by observation of a RV standard star Tau Ceti indicates about 9 m/s.

Viola속 식물의 원형질체 및 융합세포의 전자현미경 관찰 (Electron Microscopic Observations of Protoplast and Fusion Cell of Viola Species)

  • 정용모;임현희;손병구;서정해;정정한;권오창
    • 생명과학회지
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    • 제7권4호
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    • pp.282-288
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    • 1997
  • To obtain a basic information on the development of Genus Viola, ultrastructure and electrofusion process between the two protoplasts from wild Viola callus cells and pansy mesophyll cells were observed with a scanning electron microscopy(SEM) and transmission electron microscopy(TEM). In the ultrastructural observation of wild viola callus protoplasts and pansy mesophyll protoplasts using SEM, their cell walls were removed completely. A knob-like formation was observed on the enlarge surface of viola callus protoplasts. On the surface of pansy mesophyll protoplasts net-like chloroplasts were observed. In SEM observation of pansy mesophyll protoplasts, chloroplasts devoid of membrane were observed on the surface the protoplasts. Pearl chain was formed by applying AC field of 200 V/cm at 1.0 MHz for 43 sec. The lysis of plasma membranes and fusion process occurred by applying a 1,600 V/cm DC pulse twice for 1 sec. After 1-2 hours of a DC pulse application, it was observed that the two protoplasts were fused completely into one cell. In TEM observation of the fused cell, many small vacuoles were located in the fusion area of the two protoplasts. Indeed, two distinct regions were observed during fusing process; in one region, a nucleus was found, while in the other region, both nucleus and nucleous were found.

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Streptomyces mitakaensis의 원형질체 형성과정의 전자현미경적 연구 (Electron Microscopy Observation of Protoplast Formation of Streptomyces mitakaensis)

  • 한순옥;정미경;이형환
    • 한국미생물·생명공학회지
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    • 제15권2호
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    • pp.95-97
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    • 1987
  • Streptomyces mitakaensis 균사체를 lysozyme으로 처리하여 원형질체를 형성하면서 형태변이를 전자현미경으로 관찰하였다. 정상 균사체는 균사를 형성하고 포자를 형성했으며, 세포벽과 세포막이 완전한 상태로 나타났으나, lysozyme을 처리한 후에는 균사체 말단에서 단일세포가 분리되고, 세포벽이 분리되기 시작하여 30분-60분 후에는 원래 세포의 약 3배 이상 팽대한 원형질체를 관찰할 수 있었다.

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연료전지 무인항공기의 고도와 체공시간에 대한 특성 분석 및 최신 연구동향 (Research Trend and Analysis of Altitude and Endurance for Fuel Cell Unmanned Aerial Vehicles)

  • 조성현;김민진;손영준;양태현
    • 한국수소및신에너지학회논문집
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    • 제25권4호
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    • pp.393-404
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    • 2014
  • Unmanned aerial vehicles (UAVs) have been applied to not only military missions like surveillance and reconnaissance but also commercial missions like meteorological observation, aerial photograph, communication relay, internet network build and disaster observation. Fuel cells make UAVs eco-friendly by using hydrogen. Proton exchange membrane fuel cells (PEMFCs) show low operation temperature, high efficiency, low noise and high energy density and those characterisitcs are well fitted with UAVs. Thus Fuel cell based UAVs have been actively developed in the world. Recently, fuel cell UAVs have started to develope for high altitude UAVs because target altitude of UAVs is expanded upto stratosphere altitude. Long endurance of UAVs is essential to improve effects of the missions. Improvement of UAV endurance time could be fulfilled by developing a hydrogen fuel storage system with high energy density and reducing the weight of UAVs. In this paper, research trend and analysis of fuel cell UAVs are introduced in terms of their altitude and endurance time and then the prospect of fuel cell UAVs are shown.

CYTOTOXICITY OF PATULIN AND ITS EFFECT ON THE LAMBDA DNA CLEAVAGE BY RESTRICTION ENDONUCLEASE

  • Lee, Kil-Soo
    • Toxicological Research
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    • 제7권2호
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    • pp.157-163
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    • 1991
  • The effect of patulin, a mycotoxin, on the growth of Escherichia coli cell was investigated. E. coli cell elongation usually shown in SOS-response for DNA repair was induced by 20 mg of patulin per ml. After staining the E. coli chromosome with fluorescence dye(DAPI, 4', 6-diamino-2-phenyl-indole), chromosomal DNA partitioning was not affected by patulin. The observation indicateds that patulin acts as a DNA damaging agent which is effective for E. coli cell elongation introduced by the inhibition of septum formation.

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Single Cell PCR과 현미경을 통한 바지락 및 백합 유생의 동정 (Identification of Ruditapes philippinarum and Meretrix lusoria Larvae Using Single Cell PCR Analysis and Microscopic Observation)

  • 정승원;김창수;유재원;김영옥;이진환;홍재상
    • Ocean and Polar Research
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    • 제32권3호
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    • pp.247-254
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    • 2010
  • Single cell PCR analysis and light and scanning electron microscopic techniques were utilized to identify free living bivalve larvae in the coastal waters of Tae-an, on the west coast of Korea. Through DNA sequencing, venerid clam larvae were isolated and identified as Ruditapes philippinarum (99% similarity) and Meretrix lusoria (99%). Under microscopic observation, the D-veliger stage of R. philippinarum exhibited symmetrical shoulder angles and an elliptical ventral form. In contrast, M. lusoria displayed asymmetrical shoulder angles and a round ventral form in the umbonal stage. Size of the R. philippinarum larvae was $156{\pm}22{\mu}m$ in length, $126{\pm}12{\mu}m$ in height, $92{\pm}14{\mu}m$ in width with a length: height ratio of 1.23. Meretrix lusoria was $202{\pm}44{\mu}m$ in length, $161{\pm}35{\mu}m$ in height, $96{\pm}38{\mu}m$ in width with a length: height ratio of 1.25. Experimental results indicate that morphological and molecular characteristics provide evidence for the larval identification of these two venerid clam larvae species in nature.