Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of AL072, a Novel Anti-Legionella Antibiotic, on Growth and Cell Morphology of Legionella pneumophila

  • Kang, Byeong-Cheol (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Jae-Hak (Department of Laboratory Animal Science, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Yong-Soon (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Suh, Jung-Woo (R&D Center, Cheiljedang Corp.) ;
  • Chang, Jun-Hwan (R&D Center, Cheiljedang Corp.) ;
  • Lee, Chul-Hoon (Department of Medical Genetics, College of Medicine, Hanyang University)
  • Published : 1999.06.01
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Abstract

AL072 is a potent anti-Legionella antibiotic produced by Streptomyces strain AL91. The minimum inhibitory concentration (MIC) of AL072 against Legionella pneumophila was 0.2$\mu$g/ml. Bacterial growth was rapidly inhibited at the dose range between the MIC and 20 times of the MIC when the antibiotic was added at the mid-exponential phase. Ultrastructural changes in L. pneumophila were observed upon treatment with AL072. Under electron microscopical observation, the organisms treated with AL072 exhibited characteristic morphological changes in the cellular outer coat. Also irregular morphological changes, such as the formation of filamentous materials in the cytoplasm, an increase in the size and number of cytoplasmic vacuoles, the extruding of cytoplasmic contents, the formation of spheroplast and ghost cells, and blebbings in the cell wall were observed. Furthermore, immunoelectron microscopical observation of the group treated with the MIC showed that the immune complex attached mainly to the cell wall. The results of these experiments indicate that AL072, like the inhibitors of cell wall synthesis, act selectively on the cell wall of L. pneumophila.

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References

  1. Am. J. Epidemiol. v.130 Community-acquired Legionnaires disease associated with a cooling tower: Evidence for longer distance transport of Legionella pneumophila Addiss, D. G.;J. P. Davis;M. La Venture;P. J. Wand;M. A. Hutchinson;R. M. McKinney
  2. J. Med. Microbiol. v.23 The effect of antibiotics on the cell morphology of Legionella pneumophilla Chan, E. L.;R. C. Haris;H. P. Dalton
  3. J. Assoc. Off. Analytical Chem. v.71 Competitive direct enzyme linked immunosorbent assay for detection of sulfamethazine residues in swine urine and muscle tissue Dixson-Holland, D. E.;S. E. Katz
  4. N. Engl. J. Med. v.302 An outbreak of Legionnaires disease associated with a contaminated air-conditioning cooling tower Dondero, T. J. ;R. C. Rentdorff;G. F. Malllison;R. M. Weeks;J. S. Levy;E. W. Wong;W. Schaffner
  5. Lancet v.ii Evalution of ultra violet light for disinfection of hospital water contaminated with Legionella Farr, B. M.;J. C. Gratz;J. C. Tartaglino;S. I. Getchell-White;D. H. M. Groschell
  6. N. Engl. J. Med. v.297 Legionnaires disease. Description of an epidemic of pneumonia Fraster, D. W.;T. R. Tsai;W. Orenstein; W. E. Parkin;H. J. Beecham;R. G. Sharrar;J. Harris;G. F. Mallison;S. M. Martin;J. E. McDade;C. C. Shepard;P. S. Brachman;the Field Investigation Team
  7. Am. J. Public Health v.77 Pontiac fever outbreak associated with a cooling tower Friedman, S.;K. Spitalny;J. Barbaree;Y. Faur;R. MaKinney
  8. JAMA-J. Am.Med.Assn. v.254 Nosocomial Legionnaires disease: Epidemiologic demonstration of cooling tower as a source Garbe, P. L.;B. J. Davis;J. S. Weisfeld;L. Markowitz;P. Miner;F. Garrity;J. M. Barbaree;A. L. Reingold
  9. J. Clin. Microbiol. v.19 Comparison of Legionella pneumophila. L. micdadei, L. bozemanii, and L. dumoffi by transmission electron microscopy Hebert, G. A.;C. S. Callaway;E. P. Jr Ewing
  10. J. Fd. Hyg. Safety v.10 Experimental study on development of ELISA method for the detection of sulfamethazine residues Kim, S. H.;Y. K. Lim
  11. J. Antimicrob. Chemother. v.27 Inhibition of Legionella pneumophila in guinea pig peritoneal macrophages by new quinolone, macrolide and other antimicrobial agents Kitsukawa, K.;J. Hara;A. Saito
  12. FEMS Microbiol. Ecol. v.12 Biofilms as major sources of Legionella spp. in hydrothermal areas and their dispersion into stream water Marrao, G.;A. Verissimo;R. G. Bowker;M. S. daCosta
  13. J. Antimicrob. Chemother. v.22 Sensitivity and resistance of Legionella pneumophila to some antibiotics and combinations of antibiotics Moffie, B. G.;R. P. Mouton
  14. J. Clin. Microbiol. v.6 Cellular fatty acid composition of isolates from Legionnaires disease Moss, C. W.;R. E. Weever;S. B. Dees;W. B. Cherry
  15. J. Clin. Microbiol. v.9 Further studies of the celllular fatty acid composition of Legionnaires disease bacteria Moss, C. W.;S. B. Dees
  16. Appl. Environ. Microbiol. v.53 Comparative assessment of chlorine, heat. ozone, and UV light for killing Legionella pneumophila within a model plumbing system Muraca, P.;J. E. Stout;V. L. Yu
  17. J. Med. Microbiol. v.31 The effect of antibiotics that inhibit cell wall, protein, and DNA synthesis on the growth and morphology of Legionella pneumophila Rodgers, F. G.;A. O. Tzianabos;T. S. J. Elliott
  18. Bacterial Pathogenesis a molecular approach Salyers, A. A.;D. D. Whitt
  19. Zentralbl. Bakteriol. Mikrobiol. Hyg. v.[A]269 An electron microscopy study Legionella pneumophila after in vitro and in vivo culture Surgot, M.;C. M. Barioz;M. Nowicki;N. Bornstein;J. Fleurette
  20. Appl. Environ. Microbiol. v.49 Effect of temperature, pH, and oxygen level on the multiplication of naturally occurring Legionella pneumophila on potable water Wadowsky, R. M.;R. Wolford;A. M. McNamara;R. B. Yee
  21. Water Res. Bull. v.19 The stability in water of new chloramine disinfectant Worley, S. D.;W. B. Wheatley;H. H. Kohl;J. A. Van Hoose;H. D. Burkett;N. Bodor
  22. Report of the Expert Advisory Committee on Biocides Wright, A. E.
  23. J. Antibiot. v.48 AL072, A novel anti-Leginoella antibiotic produced by Streptomyces sp. Yon, C. S.;J. W. Suh;J. H. Chang;Y. H. Lim;C.-H. Lee;Y. S. Lee;Y. W. Lee