[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.3745/JIPS.04.0099

A Study on an Automatic Multi-Focus System for Cell Observation

Park, Jaeyoung (Division of Mechanics and ICT Convergence Engineering, Sunmoon University)
Lee, Sangjoon (Division of Smart Automotive Engineering, Sunmoon University)

Publication Information

Journal of Information Processing Systems / v.15, no.1, 2019 , pp. 47-54 More about this Journal

Abstract

This study is concerned with the mechanism and structure of an optical microscope and an automatic multi-focus algorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cell images quickly, a z-axis actuator with a resolution of $0.1{\mu}m$ was designed to control an optical microscope Moreover, a lighting control system was constructed to select the color and brightness of light that best suit the object being viewed. Cell images are captured by the instrument and the sharpness of each image is determined using Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are applied to automatically find the most vivid points among multiple images of a single object. A cancer cell imaging experiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained using this microscope. The proposed system is expected to save time and effort required to extract suitable cell images and increase the convenience of cell analysis.

Keywords

Automatic Multi-Focus; Cell Observation; Cubic Spline Interpolation; Peak Detection;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)

Reference
Cited By KSCI

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