• Korean Journal of Poultry Science
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• v.38 no.1
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• pp.71-80
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• 2011

This study was conducted to investigate the effects of dietary supplementation of CS682, a fermentation product of Actinomycetae Nocardia sp. CS682, and DCS682$^{(R)}$, a commercial product, on the performance, blood parameters, small intestinal microflora, and immunoglobulin contents in broilers. In Exp. 1, a total of 240 ROSS$^{(R)}$ broiler chickens of 1d old were assigned to six dietary treatments: Control, Antibiotics (6 ppm avilamycin), CS682-0.25 (CS682 0.25%), CS682-0.50, CS682-0.75 and CS682-1.00. There were significant (p<0.05) differences among treatments in feed conversion. The CS682-0.25 treatment was significantly (p<0.05) lower than Antibiotics and other CS682 treatments in 0~2 wk feed conversion. The CS682 treatments influenced MCV (mean corpuscular volume) in blood. The cfu of Escherichia coli in small intestinal content was lowest in Antibiotics treatment followed by CS682 treatments and Control. In Exp. 2, a total of 1,000 ROSS$^{(R)}$ broiler chickens of 1 d old were assigned to five dietary treatments: Control, Antibiotics (6 ppm avilamycin), DCS682-0.05 (DCS682$^{(R)}$ 0.05%), DCS682-0.10 and DCS682-0.20. There were significant differences (p<0.05) among treatments in mortality. The DCS682-0.20 treatment was lower than DCS682-0.10 in 0~3 wk and lower than Control in 0~5 wk mortality. Antibiotics treatment was lowest in all microbial population in small intestinal content. The cfu of E. coli and Salmonella typhimurium of DCS682 treatments were higher than Antibiotics treatment but lower than the Control. The results of present broiler experiments indicated that supplementation of 0.20~0.25% CS682 and DCS682, improve feed conversion, mortality and control harmful intestinal microbes.

• Korean Journal of Poultry Science
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• v.38 no.1
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• pp.59-69
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• 2011

This study was conducted to investigate the effects of dietary supplementation of CS682, a fermentation product of Actinomycetae(Nocardia sp. CS682), and its commercial product DSC682$^{(R)}$ on the performance, blood parameters, intestinal microflora, and immune response in laying hens. Hy-Line Brown$^{(R)}$ laying hens were housed in two bird cages. Feeding trial lasted 5 wk under 16.5 h:7.5 h(L:D) lighting regimen. In Exp.1, a total of 480 birds of 86 wk old were assigned to four dietary treatments: Control, Antibiotics (6 ppm avilamycin), CS682-0.1 (CS682 0.1%) and CS682-1.0 (CS682 1.0% supplementation). Each treatment was replicated five times with 24 birds (or 12 cages) per replication. In Exp. 2, a total of 1,000 birds of 26 wk old were assigned to five dietary treatments: Control, Antibiotics (6 ppm avilamycin), DCS682-0.05 (DCS682 0.05%), DCS682-0.1 (DCS682 0.1%), DCS682-0.2 (DCS682 0.2% supplementation). Each treatment was replicated five times with 40 birds (or 20 cages) per replication. In Exp. 1, there were no significant differences among treatments in egg production, egg weight, broken & soft egg production, feed intake, and feed conversion ratio. Also, there were no significant differences among treatments in eggshell thickness, eggshell color and Haugh unit. However, eggshell strength was significantly (p<0.05) greater in CS682 and Antibiotics treatments than Control, and egg yolk color was significantly (p<0.05) higher in CS682-1.0 than Control. In Exp. 2, feed intake was significantly (p<0.05) lower in DSC682-0.05 than Control. Lightness(L) of Hunter Lab color of eggshell of DCS and Antibiotics treatments was significantly (p<0.05) lower than Control. Egg yolk color of DCS 0.1 and 0.2 treatments was significantly (p<0.05) higher than Control. Haugh unit increased significantly (p<0.05) in Antibiotics and DCS682-0.1 treatments. The immunoglobulin levels of plasma (IgG and IgA) and eggyolk (IgY) were not significantly affected by treatments. Antibiotics and CS682 or DCS682 treatments significantly (p<0.05 or 0.01) influenced some of the erythrocytes and leukocytes parameters in blood. In Exp.1, mean corpuscular volume (MCV) decreased by CS682 treatments and mean corpuscular hemoglobin (MCH) was highest in Antibiotics treatments. In Exp.2, the level of monocyte (MO) decreased in DCS682-0.10 and 0.20 treatments. The cfu of C. perfringens and S. typhimurium in small intestinal content were highest in Control and lowest in Antibiotics in both experiments. In Exp. 2, DSC682-0.05 and -0.1 treatments were highest and Antibiotic treatment was lowest in Lactobacilli spp. The results of the present layer experiments indicated that supplementation of 0.1~0.2% CS682 or DCS682 may increase eggshell strength, color of eggshell and eggyolk, Haugh unit, and control harmful intestinal microbes.

• Journal of Life Science
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• v.20 no.2
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• pp.231-236
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• 2010

Antibiotics in veterinary medicine have been used to treat disease, promote growth rate and improve feed efficiency. However, alternative sources are needed because of of bacterial resistance to antibiotics and residues of antibiotics. The present study was carried out to evaluate the effect of CS682, a fermentation product of Korean soil bacteria, on safety, growth rate and feed efficiency, and to evaluate its value as an alternative for antibiotics used as a feed additive. Two dosages of CS682 (0.1% and 1%) were fed to chickens for 28 days. The results showed that, when compared to chickens in the control group, growth and feed efficiency was improved. Also, mortality, hematology, general clinical signs and necropsy were examined. Chickens in the treatment groups showed no adverse effects. A total of 72 weaning pigs were used to confirm the effects of CS682 at one dose level (0.1%) regarding feed efficiency. Supplementation with 0.1% CS682 also resulted in improved weight gain and feed efficiency ratio. Based on these results, CS682 may be effective in improving feed efficiency safely as a feed additive.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1127-1132
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• 2012

Nargenicin $A_1$ is a 28-membered polyketide macrolide, with antibacterial activity against methicillin-resistant Staphylococcus aureus, produced by Nocardia sp. CS682. In this study, the production of nargenicin $A_1$ was improved by enhancing the supply of different biosynthetic precursors. In Nocardia sp. CS682 (KCTC11297BP), this improvement was ~4.62-fold with the supplementation of 30 mM methyl oleate, 4.25-fold with supplementation of 15mM sodium propionate, and 2.81-fold with supplementation of 15 mM sodium acetate. In Nocardia sp. metK18 and Nocardia sp. CS682 expressing S-adenosylmethionine synthetase (MetK), the production of nargenicin $A_1$ was improved by ~5.57-fold by supplementation with 30 mM methyl oleate, 5.01-fold by supplementation with 15 mM sodium propionate, and 3.64-fold by supplementation with 15 mM sodium acetate. Furthermore, supplementing the culture broth of Nocardia sp. ACC18 and Nocardia sp. CS682 expressing the acetyl-CoA carboxylase complex (AccA2 and AccBE) with 30 mM methyl oleate, 15 mM sodium propionate, or 15 mM sodium acetate resulted in ~6.99-, 6.46-, and 5.58-fold increases, respectively, in nargenicin $A_1$ production. Our overall results showed that among the supplements, methyl oleate was the most effective precursor supporting the highest titers of nargenicin $A_1$ in Nocardia sp. CS682, Nocardia sp. metK18, and Nocardia sp. ACC18.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.24 no.5
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• pp.682-685
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• 2020

In this paper, we analyze the dominant noise source of conventional inductively degenerated common-source (CS) cascode low noise amplifier (LNA) when width and gate length of stacked transistors vary. Analytical MOSFET and its noise model are used to estimate the contributions of noise sources. All parameters are based on measured data of 60nm, 90nm and 130nm CMOS devices. Based on the noise analysis for different frequencies and device parameters including process nodes, the dominant noise source can be analyzed to optimize noise figure on the configuration. We verified analytically that the intuctively degenerated CS topology can not sustain its benefits in noise above a certain operation frequency of LNA over different process nodes.

• Journal of Microbiology and Biotechnology
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• v.33 no.7
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• pp.949-954
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• 2023

Type III polyketide synthase (PKS) found in bacteria is known as 1,3,6,8-tetrahydroxynaphthalene synthase (THNS). Microbial type III PKSs synthesize various compounds that possess crucial biological functions and significant pharmaceutical activities. Based on our sequence analysis, we have identified a putative type III polyketide synthase from Nocardia sp. CS682 was named as ThnA. The role of ThnA, in Nocardia sp. CS682 during the biosynthesis of 1,3,6,8 tetrahydroxynaphthalene(THN), which is the key intermediate of 1-(α-L-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene (IBR-3) was characterized. ThnA utilized five molecules of malonyl-CoA as a starter substrate to generate the polyketide 1,3,6,8-tetrahydroxynaphthalene, which could spontaneously be oxidized to the red flaviolin compound 2,5,7-trihydroxy-1,4-naphthoquinone. The amino acid sequence alignment of ThnA revealed similarities with a previously identified type III PKS and identified Cys¹³⁸, Phe¹⁸⁸, His²⁷⁰, and Asn³⁰³ as four highly conserved active site amino acid residues, as found in other known polyketide synthases. In this study, we report the heterologous expression of the type III polyketide synthase thnA in S. lividans TK24 and the identification of THN production in a mutant strain. We also compared the transcription level of thnA in S. lividans TK24 and S. lividans pIBR25-thnA and found that thnA was only transcribed in the mutant.

• Proceedings of the Korean Nuclear Society Conference
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• 1998.05b
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• pp.677-682
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• 1998

영광원전주변 해양에서 조사된 환경방사능 조사결과를 토대로 Cs-137과 Sr-90 방사성물질의 해수와 부유물에서 분포특성과 해양생물로의 전이.농축특성을 분석하였다. 방사성물질의 분포특성 분석은 해양에서 방사성물질의 용해성과 부유물에의 흡착성 평가뿐 아니라 방사성물질의 해양확산을 평가하는데 필수적 요소이다. 지금까지는 방사성물질의 해양확산 평가시 완전 용해성으로 가정하여 단순한 해수유동 특성만을 고려하여 평가하였으나, 흡착성 등 물리화학적 거동특성을 평가함으로써 좀더 사실적인 해양확산을 평가할 수 있다. 평가결과 Cs-137과 Sr-90의 분포특성을 나타내는 분배계수가 각각 8.1$\pm$1.4E-4, 7.4$\pm$2.3E-5 로 나타났다. 이는 두 핵종 모두 용해성이 높고 흡착성이 낮음을 보여준다. 그리고 Cs-137과 Sr-90에 비해 상대적으로 흡착성이 높게 나타나고 있다. 또 전이.농축특성 분석결과는 김에서 Cs-137과 Sr-90의 전이.농축계수가 66과 3, 서대와 병어에서는 122.5와 6, 패류에서는 Sr-90의 전이.농축계수가 6으로 나타났다. Sr-90은 전반적으로 전이.농축계수가 낮게 나타나 생물체에유입되더라도 쉽게 배출돼 축적경향이 매우 낮음을 보여준다. 반면 Cs-137은 Sr-90에 비해 상대적으로 농축특성이 높게 나타나고 있다. 향후 이를 토대로 해역의 고유 환경특성에 맞는 방사성물질의 해양중 거동특성을 고려한 해양확산평가 및 해양감시가 이뤄져야할 것이다.

• Nuclear Engineering and Technology
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• v.45 no.5
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• pp.675-682
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• 2013

The sequential separation process, composed of an oxygen sparging process for separating lanthanides and a zone freezing process for separating Group I and II fission products, was evaluated and tested with a surrogate eutectic waste salt generated from pyroprocessing of used metal nuclear fuel. During the oxygen sparging process, the used lanthanide chlorides (Y, Ce, Pr and Nd) were converted into their sat-insoluble precipitates, over 99.5% at $800^{\circ}C$; however, Group I (Cs) and II (Sr) chlorides were not converted but remained within the eutectic salt bed. In the next process, zone freezing, both precipitation of lanthanide precipitates and concentration of Group I/II elements were preformed. The separation efficiency of Cs and Sr increased with a decrease in the crucible moving speed, and there was little effect of crucible moving speed on the separation efficiency of Cs and Sr in the range of a 3.7 - 4.8 mm/hr. When assuming a 60% eutectic salt reuse rate, over 90% separation efficiency of Cs and Sr is possible, but when increasing the eutectic salt reuse rate to 80%, a separation efficiency of about 82 - 86 % for Cs and Sr was estimated.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.66 no.4
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• pp.682-691
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• 2017

In this paper, we propose the fusion design methodology of both pedestrian detection and object tracking system realized with the aid of HOG-PCA based RBFNN pattern classifier. The proposed system includes detection and tracking parts. In the detection part, HOG features are extracted from input images for pedestrian detection. Dimension reduction is also dealt with in order to improve detection performance as well as processing speed by using PCA which is known as a typical dimension reduction method. The reduced features can be used as the input of the FCM-based RBFNNs pattern classifier to carry out the pedestrian detection. FCM-based RBFNNs pattern classifier consists of condition, conclusion, and inference parts. FCM clustering algorithm is used as the activation function of hidden layer. In the conclusion part of network, polynomial functions such as constant, linear, quadratic and modified quadratic are regarded as connection weights and their coefficients of polynomial function are estimated by LSE-based learning. In the tracking part, object tracking algorithms such as mean shift(MS) and cam shift(CS) leads to trace one of the pedestrian candidates nominated in the detection part. Finally, INRIA person database is used in order to evaluate the performance of the pedestrian detection of the proposed system while MIT pedestrian video as well as indoor and outdoor videos obtained from IC&CI laboratory in Suwon University are exploited to evaluate the performance of tracking.

• Korean Journal of Organic Agriculture
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• v.22 no.3
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• pp.469-481
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• 2014

IIn vitro antimicrobial and anti-inflammatory activities of nargenicin and its derivatives were investigated. Nargenicin, an unusual macrolide antibiotic with potent anti-MRSA (methicilin-resistant Staphylococcus aureus) activity, was purified from the culture broth of Nocardia sp. CS682. And variety of novel nargenicin derivatives was synthesized from nargenicin. Two compounds (4 and 5) exhibit a broad spectrum of antimicrobial activities against infectious bacteria. The antimicrobial activity of derivatives against fifteen organisms was assessed using the minimum inhibitory concentration (MIC). The MIC values were in the ranges of $0.15{\sim}80{\mu}g/mL$ (w/v) for compound 1 and 2, $5{\sim}80{\mu}g/mL$ (w/v) for compound 3, $1.25{\sim}40{\mu}g/mL$ (w/v) for compound 4, and $1.25{\sim}80{\mu}g/mL$ (w/v) for compound 5, depending on the pathogens studied. In vitro, we investigated cytotoxicity and inhibition of nitric oxide (NO) production of synthesized compounds 1-5 in Raw 264.7 cells. LPS-induced nitric oxide releases were significantly blocked by compound 3, 4 and 5 in a dose-dependent manner. At high concentrations ($5{\mu}g/mL$) compound 5 inhibited the NO production by 95%. Compound 4 inhibited the release of NO in LPS-activated Raw 264.7 cells by 75% at the concentration of $10{\mu}g/mL$. Compound 3 inhibited the release of NO in LPS-activated Raw 264.7 cells by 65% at the concentration of $100{\mu}g/mL$. On the other hand, nargenicin, compound 1 and 2 did not inhibit NO production. These results demonstrated that compound 4 and 5 displayed antimicrobial activity and blocked LPS-induced pro-inflammatory mediators such as NO in macrophages, which might be responsible for its therapeutic application.