Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effect of Supplementary Actinomycetes (Nocardia sp. CS682) Ferment on the Laying Performance, Blood Parameters, Immunoglobulin and Small Intestinal Microflora Contents in Laying Hens

방선균(Nocardia sp. CS682) 발효물의 급여가 산란계의 생산성, 혈액성상, 면역글로불린 및 소장내 미생물 함량에 미치는 영향

  • Rhee, Ah-Reum (Department of Animal Science and Technology, Chung-Ang University) ;
  • Shin, Dong-Hun (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kim, Chan-Ho (Department of Animal Science and Technology, Chung-Ang University) ;
  • Jung, Byoung-Yun (Department of Poultry Science, The University of Georgia) ;
  • Yoo, Jin-Chul (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Hong, Young-Ho (Department of Animal Science and Technology, Chung-Ang University) ;
  • Paik, In-Kee (Department of Animal Science and Technology, Chung-Ang University)
  • Received : 2010.12.24
  • Accepted : 2011.03.03
  • Published : 2011.03.31
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Abstract

This study was conducted to investigate the effects of dietary supplementation of CS682, a fermentation product of Actinomycetae(Nocardia sp. CS682), and its commercial product DSC682$^{(R)}$ on the performance, blood parameters, intestinal microflora, and immune response in laying hens. Hy-Line Brown$^{(R)}$ laying hens were housed in two bird cages. Feeding trial lasted 5 wk under 16.5 h:7.5 h(L:D) lighting regimen. In Exp.1, a total of 480 birds of 86 wk old were assigned to four dietary treatments: Control, Antibiotics (6 ppm avilamycin), CS682-0.1 (CS682 0.1%) and CS682-1.0 (CS682 1.0% supplementation). Each treatment was replicated five times with 24 birds (or 12 cages) per replication. In Exp. 2, a total of 1,000 birds of 26 wk old were assigned to five dietary treatments: Control, Antibiotics (6 ppm avilamycin), DCS682-0.05 (DCS682 0.05%), DCS682-0.1 (DCS682 0.1%), DCS682-0.2 (DCS682 0.2% supplementation). Each treatment was replicated five times with 40 birds (or 20 cages) per replication. In Exp. 1, there were no significant differences among treatments in egg production, egg weight, broken & soft egg production, feed intake, and feed conversion ratio. Also, there were no significant differences among treatments in eggshell thickness, eggshell color and Haugh unit. However, eggshell strength was significantly (p<0.05) greater in CS682 and Antibiotics treatments than Control, and egg yolk color was significantly (p<0.05) higher in CS682-1.0 than Control. In Exp. 2, feed intake was significantly (p<0.05) lower in DSC682-0.05 than Control. Lightness(L) of Hunter Lab color of eggshell of DCS and Antibiotics treatments was significantly (p<0.05) lower than Control. Egg yolk color of DCS 0.1 and 0.2 treatments was significantly (p<0.05) higher than Control. Haugh unit increased significantly (p<0.05) in Antibiotics and DCS682-0.1 treatments. The immunoglobulin levels of plasma (IgG and IgA) and eggyolk (IgY) were not significantly affected by treatments. Antibiotics and CS682 or DCS682 treatments significantly (p<0.05 or 0.01) influenced some of the erythrocytes and leukocytes parameters in blood. In Exp.1, mean corpuscular volume (MCV) decreased by CS682 treatments and mean corpuscular hemoglobin (MCH) was highest in Antibiotics treatments. In Exp.2, the level of monocyte (MO) decreased in DCS682-0.10 and 0.20 treatments. The cfu of C. perfringens and S. typhimurium in small intestinal content were highest in Control and lowest in Antibiotics in both experiments. In Exp. 2, DSC682-0.05 and -0.1 treatments were highest and Antibiotic treatment was lowest in Lactobacilli spp. The results of the present layer experiments indicated that supplementation of 0.1~0.2% CS682 or DCS682 may increase eggshell strength, color of eggshell and eggyolk, Haugh unit, and control harmful intestinal microbes.

본 연구는 방선균 목 노카르디아 종 CS682 균주의 발효물 CS682와 이를 바탕으로 한 제품 DSC682$^{(R)}$를 산란계에게 급여 시 산란 생산성, 난품질, 혈액 성상, 소장 내 미생물, 면역성에 미치는 효과를 알아보기 위해 실시하였다. 실험은 Hy-Line Brown$^{(R)}$ 갈색 산란계로 실시하였는데, 실험 1은 86주령, 실험 2는 26주령의 실험계를 2단 4열 2수 케이지에 수용하여 실시하였다. 실험 1은 대조구, 항생제구(avilamycin 6 ppm), CS 682-0.1(CS682 0.1%), CS682-1.0(CS682 1.0% 첨가) 등 4 처리였으며, 5반복, 반복당 24수(12케이지), 총 480수로 실시하였고, 실험2는대조구, 항생제구(avilamycin 6 ppm), DCS682-0.05(DCS682$^{(R)}$ 0.05%), DSC682-0.1(DCS682$^{(R)}$ 0.1%), 그리고 DCS682-0.2(DCS682$^{(R)}$ 0.2% 첨가) 등 5처리였으며 5반복, 반복당 40수(20케이지), 총 1,000수로 실시하였다. 실험 1에서는 산란율, 난중, 연 파란율, 사료 섭취량, 사료 전환율 등 산란 생산성에는 유의한 차이가 없었다. 난품질에서 난각 강도는 항생제구와 CSC682 처리구들이 유의적(p<0.05)으로 높았으며, 난황색은 CSC682-1.0 처리구가 유의적(p<0.05)으로 높았다. 실험 2에서는 산란 생산성에서 사료 섭취량이 DCS682-0.05구가 대조구와 비교하여 유의적(p<0.05)으로 낮았다. 난각색에서 Hunter Lab color L(Lightness) 즉 밝기가 항생제구와 DCS682 처리구들이 유의적(p<0.05)으로 낮았다. 난황색은 DCS682-0.1~0.2구들이 유의적(p<0.05)으로 높았으며, Haugh unit은 항생제구와 DCS682-0.1구가 유의적(p<0.05)으로 높았다. 실험 1과 2에서 혈장 면역글로부린(IgG, IgA) 및 난황 면역그로부린(IgY)의 함량은 처리구간에 유의한 차이가 없었다. 실험1에서 erythrocytes 중 평균 적혈구 용적(MCV)은 대조구가 유의적(p<0.05)으로 높았으며, 평균 적혈구 혈색소량(MCH)은 항생제구가 유의적으로 높았다. 실험 2에서는 leukocytes 중 단핵구(MO)에서 대조구와 DCS682-0.05구가 유의적으로(p<0.05 또는 0.01) 높았다. 실험 1에서 C. perfringens수는 항생제구에서 대조구와 비교하여 유의적으로(p<0.05) 감소하였다. S. typhimurium수는 유의적으로(p<0.05) 차이가 있었는데 항생제구가 가장 낮았고 다음으로 CS682 처리구들이었으며 대조구에서 가장 높았다. Universal bacteria, Lactobacillus 그리고 E. coli의 수는 처리간에 유의적인 차이가 없었다. 실험 2에서는 Lactobacillus 수가 DCS682-0.05와 DCS682-0.1구들에서 유의적으로(p<0.05) 높았고 다음으로 대조구와 DCS682-0.2 첨가구였으며 항생제구가 가장 낮았다. C. perfringens 수는 항생제구에서 대조구와 비교하여 유의적(p<0.05)으로 감소하였다. S. Typhimurium은 처리간에 유의적인(p<0.05) 차이가 있었는데 항생제구가 가장 낮았고 다음으로 DCS682-0.2이었으며, DCS682-0.05와 0.1구들은 대조구보다는 낮았으나 유의한(p<0.05) 차이는 없었다. 결론적으로 CS682 0.1% 또는 DCS682 0.1~0.2% 첨가는 난각 강도, 난황색, 난각색, Haugh unit를 개선시키고, 소장 내유해 미생물을 억제하는 긍정적인 효과를 보였다.

Keywords

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