• Journal of Life Science
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• v.26 no.3
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• pp.364-375
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• 2016

Post-translational modification is an efficient process to rapidly transduce external stimulus into cellular response. Ubiquitination is a typical post-translational modification which is a highly conserved process in eukaryotes. UPS (Ubiquitin/Proteasome System) mediated by the ubiquitination is to target diverse cellular proteins for degradation. Among E3 ubiquitin ligases that function as the key determinant for substrate recognition, CRL (cullin–RING E3 ubiquitin ligase) is the largest family and forms the complex composed of cullin, RBX1, adaptor and substrate receptor. Although CRL1, also known as SCF complex, has been widely researched for its biological role, the functional studies of CRL4 have been relatively elusive. In Arabidopsis, there are 119 substrate receptors named DCAF (DDB1 CUL4 Associated Factor) proteins for CRL4 and a fraction of DCAF proteins have been identified for their potential functions so far. In this paper, current understanding on structure and biological roles of plant CRL4 complexes in a diverse of cellular events is reviewed, especially focusing on CRL4 substrate receptors. Moreover, the regulatory mechanism of CRL4’s activity is also introduced. These studies will be helpful to further understand the signal transduction pathways in which such CRL4 complexes are involved and give a clue to establish the action network of entire CRL4 complexes in plants.

• Journal of Life Science
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• v.28 no.10
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• pp.1132-1139
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• 2018

The stability of diverse cellular proteins in eukaryotes is regulated via ubiquitination. Moreover, E3 ligase plays a crucial role in determining substrate specificity and transfers ubiquitins into the substrates during the ubiquitination process. As a type of multi-subunit E3 ligase, cullin4 (CUL4)-based E3 ligase (CRL4) complex is involved in a variety of cellular processes, such as hormonal and stress responses in plants. In spite of several reports on the versatile roles of CRL4 in various signalings in Arabidopsis, CRL4's function in rice has been poorly known. To learn about CRL4-mediated cellular processes in rice in more detail, OsCUL4 that exhibits the highest homology with Arabidopsis CUL4 was isolated, and its expression patterns in various tissues and in response to plant hormones and abiotic stresses were monitored. Exogenous application of ABA or cytokinin increased the transcript levels of the OsCUL4 gene. Moreover, OsCUL4 was significantly upregulated in response to drought and salt stresses. These findings imply that OsCUL4 may be functionally related to ABA- and/or cytokinin-mediated cellular responses. OsCUL4 directly interacted with OsDDB1, an adaptor protein of CRL4, indicating that OsCUL4 can act as a scaffold protein of CRL4. An expression study on the OsCUL4 gene from this report could be used as a starting point to elucidate cellular responses in which a CRL4-mediated ubiquitination process is involved in rice.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.12 no.4
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• pp.15-27
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• 2002

The purpose of this paper is to present both the specification of delta-CRL and the polices for delta CRL in order to solve the problem involved in issuing and maintaining the certificate revocation lists for the mobile communication network. If the user request to revoke the certificate issued by certification authority, the certification should be revoked and listed up in the certificate revocation list. In general, the certificate revocation list is issued regularly. Therefore PKI application should download the CRL and prove the validity of CRL. The traffic size of the exchanged traffic should be reduced for the mobile communication environment. The result if this paper can be used for the mobile communication various environments to reduce the size of CRL.

• Journal of the Korean Operations Research and Management Science Society
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• v.30 no.4
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• pp.1-14
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• 2005

We propose a VSSI-CRL(Variable Sampling Size and Samplina Interval-Conforming Run length) synthetic control chart in order to improve the statistical characteristics of both the VSSI chart and the CRL synthetic chart. The VSSI-CRL chart utilizes VSSI sampling scheme, but it produces a signal only when the CRI is less than a given limit. An algorithm for calculating the ARL(Average Run length) and ATS(Average Time to Signal) of the VSSI-CRL chart is developed by employing Markov chain method. We present some lemmas for describing the statistical characteristics of the VSSI-CRL chart under in-control state. A procedure for designing the VSSI-CRL chart is proposed based on the lemmas. Extensive comparative studios show that the VSSI-CRL chart is superior to the CRL synthetic chart or the VSSI chart in general, and is comparable to the EWMA chart in ATS performance.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.21 no.4
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• pp.161-169
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• 2011

There is a consensus in the field of vehicular network security that public key cryptography should be used to secure communications. A certificate revocation list (CRL) should be distributed quickly to all the vehicles in the network to protect them from malicious users and malfunctioning equipment as well as to increase the overall security and safety of vehicular networks. Thus, a major challenge in vehicular networks is how to efficiently distribute CRLs. This paper proposes a CRL distribution method aided by terrestrial digital multimedia broadcasting (T-DMB). By using T-DMB data broadcasting channels as alternative communication channels, the proposed method can broaden the network coverage, achieve real-time delivery, and enhance transmission reliability. Even if roadside units are not deployed or only sparsely deployed, vehicles can obtain recent CRLs from the T-DMB infrastructure. A new transport protocol expert group (TPEG) CRL application was also designed for the purpose of broadcasting CRLs over the T-DMB infrastructure.

• Korean Journal of Veterinary Research
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• v.32 no.3
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• pp.309-319
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• 1992

This study was undertaken to establish the sequence of development of ages and its time of the fetal endochondral ossification in the scapula of the Korean native cattle. This study was also designed to confirm through histological observation the earliest stages of both chondrification and ossification. Thirty eight scapulae, a series of embryos and fetuses from the pregnant Korean native cattle ranging from 11 to 110mm in crown-rump (C-R) length, were used. The following results were obtained. The ossification center was observed in the supra- and infra- spinous fossa in the 5th group (CRL 51-60mm), that was markedly ossified in the 6th group (CRL 61~70mm) by Alizarin red S stain. The chondrogenic center of scapula was observed in the 1st group (CRL 11~20mm). The primary ossification center was presented in the 4th group (CRL 41~50mm). In the 5th group(CRL 51~60mm), the endochondral ossification progressed actively. Alcianophility was markedly increased in the interterritorial matrix in the 3rd group (CRL 31~40mm. However this reaction was markedly decreased in the interterritorial matrix the adjacent portion to the marrow cavity and trabecula in the 5th group (CRL 51~60mm).

• Journal of Korean Society of Industrial and Systems Engineering
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• v.28 no.4
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• pp.85-93
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• 2005

This paper is designed a VSI ${\overline{X}}$-CRL synthetic control chart in aspect of economy. We found the optimal sampling interval and various control limit factors under various cost parameters using cost function, proposed Lorenzen and Vance. Optimal design parameters include the sample size, control limit width, sampling interval, CRL/S chart control limit; L. Comparison and analysis of cost parameters are applied between synthetic VSI ${\overline{X}}$-CRL chart and FSI ${\overline{X}}$-CRL chart. The result of this paper shows that VSI ${\overline{X}}$-CRL chart brings cost-cutting effect of 3.04% control expense less than FSI control chart. It may not be difficult to establish the optimal economic control parameters to apply the practical cost parameters in the field.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.4
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• pp.800-806
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• 2015

WAVE(Wireless Access in Vehicular Environments) system is wireless communication technology that vehicle sends and receives packets between vehicles or between vehicles and infrastructure in a high-speed mobile environment. In this study, we have designed and implemented a CRL(Certificate Revocation List) download protocol that is used to verify certificate revocation status of the other party when the vehicles communicate with WAVE system. This protocol operates over UDP. And to support security features, also, ECDSA(Elliptic Curve Digital Signature Algorithm) is used for mutual authentication and ECIES(Elliptic Curve Integrated Encryption Scheme) is used to ensure the confidentiality. Moreover, this protocol ensures the integrity of data by adding MAC(Message Authentication Code) to the end of packet and support the error and flow control mechanisms.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.14 no.4
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• pp.25-37
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• 2004

A certificate is expected to use for its entire validity period. However, a false information record of user and compromise of private key may cause a certificate to become invalid prior to the expiration of the validity period. The CA needs to revoke the certificate. The CA periodically updates a signed data structure called a certificate revocation list(CRL) at directory server. but as CA updates a new CRL at directory server. the user can use a revoked certificate. Not only does this paper analyzes a structure of CRL and a characteristic of certificate status conviction, OCSP method but also it proposes a new certificate status verification method adding an observer information in handshake process between user and server.

• Microbiology and Biotechnology Letters
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• v.28 no.4
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• pp.223-227
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• 2000

A novel two-step acetone treatment method was developed to enhance the enantioselectivity of Candida rugosa lipase (CRL) toward the hydrolysis of racemic naproxen methyl ester. The acetone-teated CRL was considerably more enantioselective than the crude CRL, yielding an enantiomeric excess of 98~100%. The crude and acetone-treated CRLs were subjected to anion exchange chromatography, and their chromatography profiles were compared. In consequence, both chromatography profiles were found to be almost identical, resulting in two separate lipase peaks (lipase A and B). The lipase B, which is known to be less enantioselective, was treated with acetone using a two-step treatment method. The enantioselectivity of acetone-treated lipase B was dramatically increased, yielding an enantiomeric excess of 99%.