• Title/Summary/Keyword: C.C.M system

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Production of Monoclonal Antibodies Specific to Korean Mistletoe pectin (KML-C) and Their Characterization (한국산 겨우살이 렉틴 (KML-C)에 대한 단일크론항체의 생산과 특성)

  • 윤택준;유영춘;강태봉;김성훈;김갑수
    • YAKHAK HOEJI
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    • v.45 no.2
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    • pp.180-189
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    • 2001
  • We have reported that water-extracted Korean mistletoe (KM-110) had various biological activities such as antitumor and immunomodulatory activity, and the pectin fraction (KML-C) of the extract was one of major factors related to its biological functions. In this paper, we produced murine monoclonal antibody (mAb) against KML-C. The cAbs obtained were largely classified into two groups according to specificity to KML-C and ML-I, a pectin from European mistletoe. One group mAbs (9H7-D10 and 3C2-lH4) strongly reacted with KML-C, but not ML-I. In contrast, another group cAbs (8Bll-2C5, BE12-3E9 and 5E10-Fl) reacted with both KML-C and ML-1. The subisotypes of these mobs were shown to be IgGl (9H7-lD10, 3C2-lH4 and 8Bll-2C5) or IgM (8E12-3E9 and 5E10-Fl). To develop an assay system for determination of the amount of KML-C, we established the sandwich ELISA (enzyme-linked immunosorbent assay) method using these mAbs and horse radish peroxidase (HRP)-labelled cAbs. In various combinations of the cAbs for coated antibody and detection antibody, the sandwich ELISA quantitatively detected KML-C, showing the detection limit ranging from 7-5,000 ng/ml. Especially reproducibility (C.V) of the sandwich ELISA, in which 8E12-3E9 was used for coating antibody and 8Bll-2C5-HRP for detection antibody, was 4.59-5.83 in intra assay, and 3.9-9.4 in inter assay.

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c-fos mRNA Expression in the Vestibular System following Hypergravity Stimulation in Rats

  • Jin Guang-Shi;Lee Jae-Hyo;Lee Jae-Hee;Lee Moon-Young;Kim Min-Sun;Jin Yuan Zhe;Song Jeong-Hoon;Park Byung-Rim
    • The Korean Journal of Physiology and Pharmacology
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    • v.11 no.1
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    • pp.1-7
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    • 2007
  • Altered environmental gravity, including both hypo- and hypergravity, may result in space adaptation syndrome. To explore the characteristics of this adaptive plasticity, the expression of immediate early gene c-fos mRNA in the vestibular related tissues following an exposure to hypergravity stimulus was determined in rats. The animals were subjected to a force of 2 g (twice earth's gravity) for 1, 3, or 12 h, and were examined poststimulus at 0, 2, 6, 12, and 24 h. RT-PCR (reverse transcription polymerase chain reaction) and real-time quantitative RT-PCR were adopted to analyze temporal changes in the expression of c-fos mRNA. The hypergravity stimulus increased the expression of c-fos mRNA in the vestibular ganglion, medial vestibular nucleus, inferior vestibular nucleus, hippocampus, cerebellum, and cortex. The peak expression occurred at 0 h poststimulation in animals stimulated with hypergravity for 1 h, and at 6 h poststimulus in those stimulated for 3 h. In contrast, those stimulated for 12 h exhibited dual peaks at 0 and 12 h poststimulus. Bilateral labyrinthectomy markedly attenuated the degree of c-fos mRNA expression. Glutamate receptor antagonist also dramatically attenuated the degree of c-fos mRNA expression. These results indicate that expression of c-fos mRNA in response to hypergravity occurs in the vestibular related tissues of the central nervous system, in which peripheral vestibular receptors and glutamate receptors play an important role. The temporal pattern of c-fos mRNA expression depended on the duration of the hypergravity stimulus.

Study on the Southern Coastal Waters of Korea by NOAA Image (NOAA영상자료에 의한 한국 남해안연안수 조사연구)

  • 김복기
    • Korean Journal of Remote Sensing
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    • v.5 no.1
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    • pp.57-67
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    • 1989
  • This study on the southern coastal waters of Korea has been made by analysis of NOAA image and oceanographic observation data from October 1987 to August 1988. The results obtained from the study are as follow: Horizontal distributions of water temperature in different layers in winter ranged from 6.07 to 18.62$^{\circ}C$ at 0m layer, 6.02 to 18.54$^{\circ}C$ at 30m layer and 7.19 to 18.69$^{\circ}C$ at 50m layer. Consequently its vertical distribution showed homogeneity. Horizontal water temperature gradients were 0.28$^{\circ}C$/mile between the coastal waters and Tsushima warm waters. In summer, its horizontal distribution varied from 19.37 to 29.92$^{\circ}C$ at 0m layer, 13.26 to 27.11$^{\circ}C$ at 30m layer and 7.36 to 26.6$0^{\circ}C$ at 50m layer, and its vertical profile showed stratified structure. Vertical water temperature gradients were 0.44$^{\circ}C$/m between 30 and 50m layers. It was remarkable that distribution of southern coastal water system analysed by NOAA image coincided with relatively the oceanographic observation data but SST from NOAA image seemed to be 2-4$^{\circ}C$ lower in winter and 4-6$^{\circ}C$ lower in summer than the oceanographic data.

A Study on the Stability of Langmuir-Blodgett(LB) Films of Saturated Fatty Acid Monolayer (포화지방산 단분자층 LB막의 안정성에 관한 연구)

  • Park, Keun-Ho
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.3
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    • pp.352-358
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    • 2014
  • We were investigated the stability through the electrochemical characteristics of saturated fatty acid(C12, C14, C16, C18) monolayer LB films by cyclic voltammetry. Saturated fatty acid monolayer LB films was deposited on the indium tin oxide(ITO) glass by the LB method. The electrochemical properties were measured by cyclic voltammetry with a three-electrode system in 0.1 N $NaClO_4$ solution. The measuring range was continuously oxidized to 1650 mV, with an initial potential of -1350 mV was reduced. Scanning rates of 50, 100, 150, 200, and 250 mV/s were set. As a result, LB monolayer films of saturated fatty acid were appeared on irreversible processes by the oxidation current from the cyclic voltammogram. Diffusion coefficient(D) of saturated fatty acid(C12, C14, C16, and C18) was calculated 22.231, 2.461, 7.114 and 2.371 ($cm^2s^{-1}{\times}10^{-4}$) in 0.1 N $NaClO_4$ solution, respectively.

The Simplified Air Barrier System in the Perimeter Area of Building (간이형 에어베리어 시스템 적용사례 분석)

  • Cho, Jin-Kyun;Shin, Seon-Joon;Cha, Ji-Hyoung;Sung, Jae-Ho;Hong, Min-Ho
    • Proceedings of the SAREK Conference
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    • 2008.06a
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    • pp.906-911
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    • 2008
  • Because the perimeter of buildings is strongly influenced by solar and the outdoor air temperature, the area has different environmental properties compared to the interior of a building, as in summer heat gain, and in winter heat loss. In particular, if the external wall is glass, the characteristics of the glass material make it pervious to outside conditions, thereby making big changes to the thermal environment. By combining shading device and the efficient exhaust system, an energy saving can be achieved compared to no air barrier systems. The simplified air barrier system is developed with the idea that energy could be conserved by carefully and effectively blowing out the air caught between the glass surface and the roller blind. The way it is configured is therefore by making the roller blind's air-path, and by placing the air output ducts in the most optimum positions. This simplified air barrier system will give improvement in the thermal environment of the parameter area that is strongly affected by solar and the outdoor condition.

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Success Factor of m-commerce; A case of DoCoMo′s i-Mode Service (기술언어가 m-commerce의 성공에 미치는 영향에 관한 소고 - i-mode 서비스를 중심으로 -)

  • 김광희
    • Proceedings of the Korea Inteligent Information System Society Conference
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    • 2001.06a
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    • pp.305-314
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    • 2001
  • 본 논문해서는 m-커머스의 대표적인 성공 사례로 꼽히고 있는 NTT DoCOMo의 i-mode 서비스에 초점을 맞추어 성공요인과 그러한 성공을 이끌어 내는데 결정적인 역할을 담당한 웹 페이지 기술언어에 대해 살펴보았다. 분석 결과에 근거하면, i-mode 서비스의 대대적인 성공은 끊임없이 Positive Feedback의 계기를 만들어 냄으로써 가능하게 되었는데, 그것은 다름 아닌 C-HTML이라는 웹페이지 기술언어의 채용이 많은 역할을 하고 있다.

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The Development of Fertical type LPE System for Single Crystal Growth (단결정 성장을 위한 수직형 LPE 장치의 제작)

  • 오종환
    • Proceedings of the Optical Society of Korea Conference
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    • 1989.02a
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    • pp.201-205
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    • 1989
  • In this study, the vertical type LPE system has been developed by fully hand-made. The temperature fluctuation and minimum cooling rate of this LPE system are within $\pm$0.05$^{\circ}C$ and 0.15$^{\circ}C$/min, respectively. It is considered that these properties are enough to grow III-V semiconductor compounds single crystals by liquid phase epitaxy method. Futhermore in this study. 1.3${\mu}{\textrm}{m}$ GaInAsP/InP single crystal growing has been carried out by this system. It has been obtained that the growing rate was about 0.72${\mu}{\textrm}{m}$/min for InP binary layer and 0.36${\mu}{\textrm}{m}$/min for GaInAsP quarternary layer under 0.6$^{\circ}C$/min cooling rate condition.

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Fabrications and Characteristics of Microfluidic Systems Actuated by Thermopneumatic Method (열공압 방식으로 구동되는 매세 유체 제어 시스템의 제작 및 특성)

  • Yoo Jong-Chul;Kang C. J.;Kim Yong-Sang
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.55 no.2
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    • pp.88-92
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    • 2006
  • We present a microfluidic system with microvalves and a micropump that are easily integrated on the same substrate using the same fabrication process. The fabricated microfluidic system is suitable for use as a disposable device and its characteristics are optimized for use as a micro chemical analysis system (micro-TAS) and lab-on-a-chip. The system is realized by means of a polydimethylsiloxane (PDMS)-glass chip and an indium tin oxide (ITO) heater. We demonstrate the integration of the micropump and microvalves using a new thermopneumatic-actuated PDMS-based microfluidic system. A maximum pumping rate of about 730 nl/min is observed at. a duty ratio of 1 $\%$ and a frequency of 2 Hz with a fixed power of 500 mW. The measured power at flow cut-off is 500 mW for the microvalve whose channel width, depth and membrane thickness were 400 $\mu$m, 110 $\mu$m, and 320 $\mu$m, respectively.

Low System Complexity Bit-Parallel Architecture for Computing $AB^2+C$ in a Class of Finite Fields $GF(2^m)$ (시스템 복잡도를 개선한 $GF(2^m)$ 상의 병렬 $AB^2+C$ 연산기 설계)

  • 변기령;김흥수
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.40 no.6
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    • pp.24-30
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    • 2003
  • This study focuses on the arithmetical methodology and hardware implementation of low system-complexity A $B^2$+C operator over GF(2$^{m}$ ) using the irreducible AOP of degree m. The proposed parallel-in parallel-out operator is composed of CS, PP, and MS modules, each can be established using the array structure of AND and XOR gates. The proposed multiplier is composed of (m+1)$^2$ 2-input AND gates and (m+1)(m+2) 2-input XOR gates. And the minimum propagation delay is $T_{A}$ +(1+$\ulcorner$lo $g_2$$^{m}$ $\lrcorner$) $T_{x}$ . Comparison result of the related A $B^2$+C operators of GF(2$^{m}$ ) are shown by table, It reveals that our operator involve more lower circuit complexity and shorter propagation delay then the others. Moreover, the interconnections of the out operators is very simple, regular, and therefore well-suited for VLSI implementation.