YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Production of Monoclonal Antibodies Specific to Korean Mistletoe pectin (KML-C) and Their Characterization

한국산 겨우살이 렉틴 (KML-C)에 대한 단일크론항체의 생산과 특성

  • 윤택준 (한동대학교 생의학연구소, 경희대학교 동서의학대학원 종양연구팀) ;
  • 유영춘 (건양대학교 의과대학 미생물학 교실) ;
  • 강태봉 (한동대학교 생의학연구소) ;
  • 김성훈 (경희대학교 동서의학대학원 종양연구팀) ;
  • 김갑수 (카프리바이오텍 주식회사)
  • Published : 2001.04.01
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Abstract

We have reported that water-extracted Korean mistletoe (KM-110) had various biological activities such as antitumor and immunomodulatory activity, and the pectin fraction (KML-C) of the extract was one of major factors related to its biological functions. In this paper, we produced murine monoclonal antibody (mAb) against KML-C. The cAbs obtained were largely classified into two groups according to specificity to KML-C and ML-I, a pectin from European mistletoe. One group mAbs (9H7-D10 and 3C2-lH4) strongly reacted with KML-C, but not ML-I. In contrast, another group cAbs (8Bll-2C5, BE12-3E9 and 5E10-Fl) reacted with both KML-C and ML-1. The subisotypes of these mobs were shown to be IgGl (9H7-lD10, 3C2-lH4 and 8Bll-2C5) or IgM (8E12-3E9 and 5E10-Fl). To develop an assay system for determination of the amount of KML-C, we established the sandwich ELISA (enzyme-linked immunosorbent assay) method using these mAbs and horse radish peroxidase (HRP)-labelled cAbs. In various combinations of the cAbs for coated antibody and detection antibody, the sandwich ELISA quantitatively detected KML-C, showing the detection limit ranging from 7-5,000 ng/ml. Especially reproducibility (C.V) of the sandwich ELISA, in which 8E12-3E9 was used for coating antibody and 8Bll-2C5-HRP for detection antibody, was 4.59-5.83 in intra assay, and 3.9-9.4 in inter assay.

Keywords

References

  1. Jpn. J. Cancer Res. v.89 Oral administration of Kampo (Japans Herbal) medicine Juzen-taito-to inhibits metastasis of colon 26L5 carcinoma cells Dhmashi, Y.;Fujii, H.;Hayakawa, Y.;Sakukawa, R.;Yamarura, T.;Sukamoto, T.;Tsukada, K.;Fujimaki, M.;Numome, S.;Komatsu, Y.;Saiki, I.
  2. J. immunol. v.163 Monoclonal antibodies that distinguish between two related digitals glycosides, Ouabain and Digoxin Parhami-Seren, B.;Bell, C.;Margolies, M.N.;Haupert, G.T.Jr.
  3. Lap Invest. v.44 Biologic properties of Iscador;A Viscum album proparation;Ⅰ. Hyperplasia of thr thymic cortex and accelerated regeneration of hematopoietic cells following X-irradiation Rentea, R.;Lyon, E.;Hunter, R.
  4. Immunopharmacology v.17 Biochemical characterization of a component in extracts of Viscum album enhancing human NK cytotoxicity Mueller, E.A.;Hamprecht, K.;Anderer, F. A.
  5. Kor. J. Pharmcogn. v.25 Effect of Korean mistletoe extracts on the induction of IL-1 and TNF-a from mouse macrophages Yoon, T.J.;Yoo, Y.C.;Hong, E.K.;Cho, Y.H.;Lee, S.W.;Azuma, I.;Yoo, B.L.;Kim, J.B.
  6. Anticancer Res. v.18 Mistletoe in immunology and the clinic Stein, G.M.;Schietzel, M.;Bussing, A.
  7. Anticancer Drugs. v.8 no.S53(Sup.) Clinical relevance of immunoactive mistletoe lectin-Ⅰ Beuth, J.
  8. J. Biol Regul Homeost Agents. v.7 Mistletoe (Viscum album) lectins as cytokine inducers and immunoadjuvant in tumor therapy Bocci, V.
  9. Cancer Lett. v.97 Inhibitory effect of Korean mistletoe(Viscum album coloratum) extract on tumour angiogenesis and metastasis of haematogenous and non-haematogenous tomour cells in mice Yoon, T.J.;Yoo, Y.C.;Choi, O.B.;Do, M.S.;Kang, T.B.;Lee, S.W.;Azuma, I.;Kim, J.B.
  10. Int. J. Immunpharmacol. v.20 Prophylactic effect of Korean mistletoe (Viscum album coloratum) extract on tumor metastasis is mediated by enhancement of NK cell activity Yoon, T.J.;Yoo, Y.C.;Kang, T.B.;Baek. Y.J.;Huh, C.S.;Song, S.K.;Lee, K.H. Azuma, I.;Kim, J.B.
  11. Cancer Lett. v.136 Lectins isolated from Korean mistletoe (Viscum album coloratum) induce apoptosis in tumor cells Yoon, T.J.;Yoo, Y.C.;Kang, T. B.;Shimazaki, K.;Song, S.K.;Lee, K.H.;Kim, S.H.;Park, C.H.;Azuma, I.;Kim, J.B.
  12. Oncology v.43 no.sup. Mistletoe lectins and their A and B chains Franz, H.
  13. Anticancer Drugs. v.8 no.S43(Sup.) Immunomodulatory effects of Viscum album agglutinin-1 on natural immunity Hajto, T.;Hostanska, K.;Fischer, J.;Saller, R.
  14. Arzneimittelforschung v.49 Study of heterogeneity of lectins in mistletoe preparations by monoclonal antibodies to their A-subunits Tonevitsky, A.G.;Agapov, I.;Temiakov, D.;Moisenovich, M.;Maluchenko, N.;Solopova, O.;Wurzner, G.;Pfueller, U.
  15. Arzneimittelforschung v.45 Quantitative determination of mectins in mistletoe preparations Jaggy, C.;Musielski, H.;Urech, K.;Schaller, G.
  16. Immunol Lett. v.46 Hybridoma cells producing antibodies against A-chain of mistletoe lectin I are resistant to this toxin Tonevitsky, A.G.;Shamshiev, A.T.;Prokoph'ev, S.A.;Agapov, Ⅱ;Toptygin, AYu
  17. Immunopharmacol Immunotoxilcol. v.22 Protein kinase A or C modulates the apoptosis induced by lectin Ⅱ isolated from Korean mistletoe, Vuscum album car. Coloratum, in the human leukemic HL-60 cells Pae, H.O.;Seo, W.G.;Shin, M.;Lee, H.S.;Lee, H.S.;Kim, S.B.;Chung, H.T.
  18. Arch Pharm Res. v.21 Isolation and characterization of beta-galactoside specific lectin form Korean mistletoe(Viscum album var. coloratum) with lactose-BSA-sepharose 4B and changes of lectin conformation Park, W.B.;Ju, Y.J.;Han, S.K.
  19. Int. J. immunopharm. Cellular and humoral adjuvant activity of lectins isolated form Korean mistletoe(Viscum album colaratum) Yoon, T.J.;Yoo, Y.C.;Kang, T.B.;Kim, S-H.;Azuma, I.;Kim, J.B.
  20. Steroids v.38 Use of monoclonal antidodies to pregnanediol-3 alpha glucuronide for the development of a solid phase chemiluminesence immunoassay Eshhar, Z.;Kim, J.B.;Barnard, G.;Collins, W.P.;Gilad, S.;Lindner, H.R.;Kohen, F.
  21. Clin. Chem. v.28 Measurement of plasma estradiol-17 solid-phase chemiluminescence immunoassay Kim, J.B.;Barnard, G.J.;Collins, W.P.;Kohen, F.;Lindner, H.R.;Eshhar, Z.
  22. J. Steroid Biochem. v.27 Recent advances in chemiluminescence-based immunoassays for steroid hormones Kohen, F.;De Boever, J.;Kim, J.B.