• Title/Summary/Keyword: Buffer curve

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Growth of $CaF_{2}:R^{+3}$ (R=Nd, Er) layers by molecular beam epitaxy (Molecular beam epitaxy법에 의한 희토류 이온$(Nd^{3+},\;Er^{3+})$ 첨가 $CaF_{2}$ 박막의 성장)

  • ;Yefen Chen;Tsuguo Fukuda
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.1
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    • pp.1-5
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    • 1999
  • The rare-earth ions ($R^{3+}$, R=Nd, Er) doped $CaF_{2}$ layers have been grown on $CaF_{2}$ (111) substrate by molecular beam epitaxy. The surface structure and the crystallinity of $CaF_{2}:R^{3+}$ layers depending on the doping concentration of $R^{3+}$ and layer thickness were studied by reflection high-energy electron diffraction (RHEED). In aspect of application as buffer layer in semiconductor-related hybrid structure, the lattice displacement between $CaF_{2}:R^{3+}$ layers and $CaF_{2}$ (111) substrate was investigated by X-ray rocking curve analysis.

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Determination of Horseradish Peroxidase (HRP) using an Enhanced Chemiluminescence Assay (증강 화학발광 기법을 이용한 horseradish peroxidase(HRP)의 검량)

  • Kim, Wongee;Kim, Keunhan;Lee, Seungmok
    • Journal of Korean Society on Water Environment
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    • v.25 no.1
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    • pp.84-89
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    • 2009
  • Our nation's water resources remain susceptible to contamination by phenolic agrichemicals. These compounds can be toxic to a variety of organisms including humans. Their disposal is restricted in many countries with strict limits for acceptable concentrations in drinking water. Enzyme-mediated in situ stabilization has been advocated as an approach for the treatment of phenolic compounds in soils and groundwater. This study reports the development of a new approach to quantify the activity of the HRP enzyme in aqueous systems. The method is based on the coupled processes of energy transfer and enhanced chemiluminescence using a luminol-$H_2O_2$-HRP system. In this study, the effects of solution pH, ionic strength and aqueous concentrations of HRP, $H_2O_2$ and enhancer were evaluated on the p-iodophenol-enhanced, HRP-catalyzed chemiluminescence reaction intensity in Tris-HCl buffer. All assay components were found to affect the maximum chemiluminescene intensity. The calibration curve for HRP showed the linear relationship with maximum light intensity.

Verifing Formation of Area of Influence of Subway Station through Land Value Distribution Analysis - Case Study on Seoul

  • Lee, Byoungkil;Lee, Sangkyeong
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.34 no.4
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    • pp.403-411
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    • 2016
  • This research has the purpose to develop a method to evaluate whether station’s area of influence has been formed, and verify formation of the area of influence through empirical analysis of all subway stations in Seoul. First, we created buffers of 100m intervals from 100m to 1000m, based on subway station exits, calculated the average land price of each buffer, and divided station areas of influence into 10 clusters using K-means clustering with the average land prices as values of observation. Subsequently, we have assumed a decreasing price curve from increasing distance from a nearby subway station, estimated a price curve and evaluated whether the area of influence actually exists using regression analysis of each cluster. The 10 area of influence clusters were largely divided into strong, weak, and no area of influence of subway station. The stations where the strong areas of influence are formed are mainly located in center, sub-centers, and local centers; stations where weak and no areas of influence are formed are mostly located in the adjacent areas of center or sub-centers or suburbs.

Efficient Rate Control by Lagrange Multiplier Using Adaptive Mode Selection in Video Coding (비디오 코팅시 Lagrage 승수를 조정하여 적응 모드 선택에 따른 비트율의 제어)

  • Ryu, Chul;Kim, Seung P.
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.1B
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    • pp.77-88
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    • 2000
  • This paper presents an approach for rate control by adaptively selecting macroblock modes in video coding.The problem of rate control has been investigated by many authors where quantizer level is adjustedbased on the buffer fullness. The proposed approach is different fron the previous ones [4] id that it finds the optimal decision curve rather than finding a set of the modes. Proposed algorithm extends the coding decision options for rate control to motion/no-motion compensation as well as inter/intra decisions. Instead of having a fixed motion/no-notion compensation or inter/intra decision curve, one can utilize an adaptive decision curvebased on the characteristics of input frames so that the PSNR at a given bit rate is maximized. Therefore, the proposed approach provides better rate control than simple quantizer feedback approach interns of visual quality. The curve is obtained by utilizing simulated annealing optimization technique. Thealgorithm is implemented and simulations are compared with other approaches within H.261 video codec.

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Spectrophotometric Determination of Ion Pair Extraction of Quaternary Amines with Methyl Orange (Methyl Orange에 의한 4급 Amine류의 Ion Pair 추출 흡광도 정량(I) - Cetyltrimenthylammonium bromide, Brethlium tosylate, Gallamine triethiodide 및 Thiamine의 정량)

  • 최종환;김영수
    • YAKHAK HOEJI
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    • v.31 no.2
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    • pp.45-51
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    • 1987
  • Methyl orange(MO) and cetyltrimethylammonium bromide(CTA) were mixed and added to 10ml of the buffer solution and then the solution was shaken for 5 minutes. The maximum absorption wavelength of the reaction product was 419nm. Dichloromethane was the best extracting solvent among the several organic solvents and the most suitable pH range was 2~8. When the CTA-MO calibration curve was made in the best experimental condition, the Lambert-Beer's law was obeyed in the range of CTA concentration of $1.5\times$$10^{-5}$~1.0$\times$$10^{-4}M$ by UV spectrophotometer. This method was possible to determine quaternary ammonium salts in the pharmaceutical preparations.

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Determination of Lead(II) at Nafion-DTPA-Glycerol-Modified Glassy Carbon Electrodes

  • Park, Eun-Heui;Park, Chan-Ju;Chung, Keun-Ho
    • Proceedings of the Korean Environmental Health Society Conference
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    • 2003.06a
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    • pp.204-207
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    • 2003
  • Determination of Lead(II) using nafion-DTPA (diethylene triamine pentaacetic acid)-glycerol-modified glassy carbon electrodes is described. Lead(II) is accumulated at the electrode by complexing with the DTPA, reduced, and detected by differential pulse voltammetry. In this study, we demonstrate that at a preconcentration time of 5min the nafion-DTPA-glycerol-modified glassy carbon electrode has a linear calibration curve at range 1.0${\times}$10$\^$-9/M∼1.0${\times}$10$\^$-7/M in pH 4.0 buffer solution. The detection limit(3$\sigma$) is as low as 5.0${\times}$10$\^$-6/M. This method is applied to the determination of lead(II) in certified reference material and the result agrees satisfactorily with the certified value.

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Spectrophotometric Determination of Ion Pair Extraction of Quaternary Amines with Metanil Yellow (Metanil Yellow에 의한 4급 Amine류의 Ino Pair 추출 흡광도 정량 (II) - Benzalkonium Chloride, Cetylpyridinium Chloride 및 Dimenhydrinate의 정량)

  • 김영수;최종환
    • YAKHAK HOEJI
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    • v.35 no.1
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    • pp.15-21
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    • 1991
  • Quaternary amines which are widely used as medicines are nitrogen compounds. Metanil yellow(MY) and benzalkonium chloride(BKC) were mixed and added to 10ml of the buffer solution and then the solution was shaken for 5 minutes. The maximum absorption wavelength of the reaction product was 402 nm. Dichloromethane was the best extracting solvent among the several organic solvents and the most suitable pH range was 2~8. When the BKC-MY calibration curve was made in the best experimental condition, the Lambert-Beer's law was obeyed in the range of BKC concentration of 2$\times$$10^{-6}$~9$\times$$10^{-6}$M by UV spectrophotometer. This method was possible to determine quaternary ammonium salts in the pharmaceutical preparation.

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A Study of Structure Properties of GaN films on Si(111) by MOCVD (Si 기판을 이용한 GaN 박막의 구조적 특성 연구)

  • Kim, Deok-Kyu;Kim, Kyoung-Min;Kim, Jin-Sa;Park, Choon-Bae
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2005.11a
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    • pp.59-60
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    • 2005
  • The characteristics of GaN epitaxial layers grown on silicon (111) substrates by metalorganic vapor phase epitaxy have been investigated. The only control of AIN thickness was found to decrease the stress sufficiently for avoiding crack formation in an overgrown thick ($2.6{\mu}m$) CaN layer. X-ray diffraction measurementsare used to determine the effect of AIN thickness on the strain in the subsequent GaN layers. The 437arcsec linewidth on the (002) x-ray rocking curve also attest the high crystalline quality of GaN on Si (111).

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Alkaline Dyeing and Color Fastness of Polyester Fiber (폴리에스테르 섬유의 알칼리 염색과 견뢰도)

  • 정동석;오준석;이문철
    • Textile Coloration and Finishing
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    • v.12 no.4
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    • pp.248-255
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    • 2000
  • Polyester fibers and fabrics have been dyed with disperse dyes in alkaline dyebath such as alkaline buffer and alkaline auxiliary(JPH-95) comparing a traditional acidic dyeing. After dyeing the samples were extracted with 100% DMF, and washing and rubbing fastnesses were measured. In dyeing at $100^\circ{C}$ the dyeing rate increased with decreasing fiber denier, regardless of dye baths, whereas the dyeing rates of the same denier fiber increased in the order of alkaline dyeing>acidic dyeing>JPH-95 dyeing. In dyeing at $130^\circ{C}$ the dyeing rate of PET fiber in JPH-95 dye bath decreased compared with the other two types of dye baths. In the time and temperature curve the dye uptake of JPH-95 dyeing was higher than the other two types of dye baths in the range of low temperature$(95~115^\circ{C})$. The equilibrium dye uptake increased in the order of 0.52d>2.04d>0.05d fiber. Washing fastness had no change in all three types of dye baths. But rubbing fastness was not good for alkaline dyeing except black dyes.

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Development of Homogeneous Enzyme Immunoassay for Serum 3,5,3'-Triiodothyronine Determination (혈청 3,5,3'-Triiodothyronine 측정을 위한 효소-면역 분석의 개발 연구)

  • 이희주
    • YAKHAK HOEJI
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    • v.27 no.2
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    • pp.117-124
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    • 1983
  • For development of $EMIT-T_{3}$ assay, the conjugation of 3, 5, 3'-triiodothyroformic acid NHS ester to G6PDH was attempted in various reaction conditions. Up to now, the best conjugation condition was the ratio of $T_{3}$-NHS:G6PDH=100 in 25% carbitol-Tris buffer at pH 9, $0^{\circ}C$ during overnight. The obtained $T_{3}$-G6PDH conjugates usually had 20% residual enzyme activity which was inhibited by 40-70% with various $anti-T_{3}$ antibodies. Utilizing the conjugate I and an antibody (S2633G), a useful standard curve for $T_{3}$ assay was obtained in the range of 0 to 5ng/ml with 499 EMIT units of separation.

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