Spectrophotometric Determination of Ion Pair Extraction of Quaternary Amines with Methyl Orange

Methyl Orange에 의한 4급 Amine류의 Ion Pair 추출 흡광도 정량(I) - Cetyltrimenthylammonium bromide, Brethlium tosylate, Gallamine triethiodide 및 Thiamine의 정량

Methyl orange(MO) and cetyltrimethylammonium bromide(CTA) were mixed and added to 10ml of the buffer solution and then the solution was shaken for 5 minutes. The maximum absorption wavelength of the reaction product was 419nm. Dichloromethane was the best extracting solvent among the several organic solvents and the most suitable pH range was 2~8. When the CTA-MO calibration curve was made in the best experimental condition, the Lambert-Beer's law was obeyed in the range of CTA concentration of $1.5\times$$10^{-5}$~1.0$\times$$10^{-4}M$ by UV spectrophotometer. This method was possible to determine quaternary ammonium salts in the pharmaceutical preparations.