한국물환경학회지 (Journal of Korean Society on Water Environment)

  • 제25권1호
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  • Pages.84-89
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  • 2009
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  • 2289-0971(pISSN)
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  • 2289-098X(eISSN)
한국물환경학회 (Korean Society on Water Environment)
권호 표지

증강 화학발광 기법을 이용한 horseradish peroxidase(HRP)의 검량

Determination of Horseradish Peroxidase (HRP) using an Enhanced Chemiluminescence Assay

  • 투고 : 2008.01.23
  • 심사 : 2008.11.10
  • 발행 : 2009.01.30
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초록

Our nation's water resources remain susceptible to contamination by phenolic agrichemicals. These compounds can be toxic to a variety of organisms including humans. Their disposal is restricted in many countries with strict limits for acceptable concentrations in drinking water. Enzyme-mediated in situ stabilization has been advocated as an approach for the treatment of phenolic compounds in soils and groundwater. This study reports the development of a new approach to quantify the activity of the HRP enzyme in aqueous systems. The method is based on the coupled processes of energy transfer and enhanced chemiluminescence using a luminol-$H_2O_2$-HRP system. In this study, the effects of solution pH, ionic strength and aqueous concentrations of HRP, $H_2O_2$ and enhancer were evaluated on the p-iodophenol-enhanced, HRP-catalyzed chemiluminescence reaction intensity in Tris-HCl buffer. All assay components were found to affect the maximum chemiluminescene intensity. The calibration curve for HRP showed the linear relationship with maximum light intensity.

키워드

과제정보

연구 과제 주관 기관 : 관동대학교

참고문헌

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