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Determination of Horseradish Peroxidase (HRP) using an Enhanced Chemiluminescence Assay  

Kim, Wongee (GreenTech.Co., Ltd.)
Kim, Keunhan (Sunjin Environment Co., Ltd.)
Lee, Seungmok (Department of Environmental Engineering, Kwandong University)
Publication Information
Journal of Korean Society on Water Environment / v.25, no.1, 2009 , pp. 84-89 More about this Journal
Abstract
Our nation's water resources remain susceptible to contamination by phenolic agrichemicals. These compounds can be toxic to a variety of organisms including humans. Their disposal is restricted in many countries with strict limits for acceptable concentrations in drinking water. Enzyme-mediated in situ stabilization has been advocated as an approach for the treatment of phenolic compounds in soils and groundwater. This study reports the development of a new approach to quantify the activity of the HRP enzyme in aqueous systems. The method is based on the coupled processes of energy transfer and enhanced chemiluminescence using a luminol-$H_2O_2$-HRP system. In this study, the effects of solution pH, ionic strength and aqueous concentrations of HRP, $H_2O_2$ and enhancer were evaluated on the p-iodophenol-enhanced, HRP-catalyzed chemiluminescence reaction intensity in Tris-HCl buffer. All assay components were found to affect the maximum chemiluminescene intensity. The calibration curve for HRP showed the linear relationship with maximum light intensity.
Keywords
Calibration curve; Chemiluminescence; Horseradish Peroxidase (HRP); Ionic strength;
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1 Thorpe, G. H. G., Kricka, L. J., Moseley, S. B., and Whitehead, T. P. (1985a). Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence-monitored enzyme immunoassays. Clinical Chemistry, 31, pp. 1335-1341   PUBMED
2 Motsenbocker, M. A. and Kondo, K. (1994). Improvement to enhanced horseradish peroxidase detection sensitivity. Journal of Bioluminescence and Chemiluminescence, 9, pp. 15-20   DOI   ScienceOn
3 Nicell, J. A., Bewtra, J. K., Biswas, N., St. Pierre, C., and Taylor, K. E. (1993). Enzyme catalyzed polymerization and precipitation of aromatic compounds from aqueous solution. Canadian Journal of Civil Engineering, 20, pp. 725-735   DOI   ScienceOn
4 Borgesa, E. P. and Reis, B. F. (2005). An enzymatic flowinjection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process. Journal of Brazilian Chemical Society, 16, pp. 1226-1232   DOI   ScienceOn
5 Osman, A. M., Zomer, G., Laane, C., and Hilhorst, R. (2000). Comparative studies of the chemiluminescent horseradish peroxidase-catalyzed peroxidation of acridan (GZ-11) and luminol reactions: effect of pH and scavengers of reactive oxygen species on the light intensity of these systems. Luminescence, 15, pp. 189-197   DOI   ScienceOn
6 Ilyina, A. D., Martinez Hernández, J. L., Mauricio Benavides, J. E., Lopez Luján, B. H., Bogatecheva, E. S., Romero Garcia, J., and Rodiriguez Martínez, J. (2003). Determination of phenol using an enhanced chemiluminescent assay. Luminescence, 18, pp. 31-36   DOI   ScienceOn
7 Thorpe, G. H. G., Kricka, L. J., Gillespie, E., Moseley, S., Amess, R., Baggett, N., and Whitehead, T. P. (1985b). Enhancement of the horseradish peroxidase-catalyzed chemiluminescent oxidation of cyclic diacyl hydrazide by 6-hydroxybenzothiazoles. Analytical Biochemistry, 145, pp. 96-100   DOI   ScienceOn
8 Cormier, M. J. and Prichard, P. M. (1968). An investigation of the mechanism of the luminescent peroxidation of luminol by stopped flow techniques. Journal of Biological Chemistry, 243, pp. 4706-4714
9 Kricka, L. K. and Thorpe, G. H. G. (1983). Chemiluminescent and bioluminescent methods in analytical chemistry. Analyst, 108, pp. 1274-1293   DOI
10 Li, W., Chen, K., Zhao, Y., Chen, L., Toselli, P., Chou, I. N., and Stone, P. (2004). Transcriptional perturbation of lysyl oxidase by cigarette smoke condensate in cultured lung fibroblasts. Toxicologist, 78, pp. 262 (abstract)
11 Thorpe, G. H. G., Gillespie, E., Haggart, R., Kricka, L. J., and Whitehead, T. P. (1984). Analytical applications of bioluminescence and chemiluminescence. Academic, London
12 Ii, M., Yoshida, H., Aramaki, Y., Masuya, H., Hada, T., Terada, M., Hatanaka, M., and Ichimori, Y. (1993). Improved enzyme immunoassay for human basic fibroblast growth factor using a new enhanced chemiluminescence system. Biochemical and Biophysical Research Communications, 193, pp. 540-545   DOI   ScienceOn
13 Klibanov, A. M., Tu, T. M., and Scott, K. P. (1983). Peroxidase catalyzed removal of phenols from coal-conversion wastewaters. Science, 221, pp. 259-261   PUBMED
14 Wu, Y., Taylor, K. E., Biswas, N., and Bewtra, J. K. (1997). Comparison of additives in the removal of phenolic compounds by peroxidase-catalyzed polymerization. Water Research, 31, pp. 2699-2704   DOI   ScienceOn
15 Yasaei, P. M., Yang, G. C., Warner, C. R., Daniels, D. H., and Kau, Y. (1996). Singlet oxygen oxidation of lipids resulting from photochemical sensitizers in presence of antioxidants. Journal of the American Oil Chemists' Society, 73, pp. 1177-1181   DOI   ScienceOn
16 Thorpe, G. H. G. and Kricka, L. J. (1986). Enhanced chemiluminescent reactions catalyzed by horseradish peroxidase. Methods Enzymol., 133, pp. 331-353   DOI   PUBMED
17 Yeh, H. C. and Lin, W. Y. (2002). Enhanced chemiluminescence for the oxidation of luminol with m-chloroperoxybenzoic acid catalyzed bymicroperoxidase. Analytical and Bioanalytical Chemistry, 372, pp. 525-531   DOI   ScienceOn