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Validation of Reference Genes for Quantifying Changes in Physiological Gene Expression in Apple Tree under Cold Stress and Virus Infection (저온과 바이러스 감염에 노출된 사과나무의 생리적 유전자 정량 측정용 유전자들의 발현 분석 및 검증)

  • Yoon, Ju-Yeon;Jeong, Jae-Hoon;Choi, Seung-Kook
    • Research in Plant Disease
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    • v.26 no.3
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    • pp.144-158
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    • 2020
  • Quantitative reverse transcription PCR is used for gene expression analysis as the accurate and sensitive method. To analyze quantification of gene expression changes in apple plants, 10 housekeeping genes (ACT, CKL, EF-1α, GAPDH, MDH, PDI, THFs, UBC, UBC10, and WD40) were evaluated for their stability of expression during infection by Apple stem grooving virus (ASGV) or in cold-stress apple plant buds. Five reference-gene validation programs were used to establish the order of the most stable genes for ASGV as CKL>THFs>GAPDH>ACT, and the least stable genes WD40CKL>UBC10, and the least stable genes were ACT

Characteristics of Mulberry Cultivar "Daejappong" (Morus Lhou(Ser.) Koidz.) for Mulberry Fruit Production (오디 생산용 뽕나무 품종 "대자뽕"의 특성)

  • Sung, Gyoo Byung;Kim, Hyun-Bok;Kang, Pil Don;Kim, Ki Young;Ji, Sang Duk
    • Journal of Sericultural and Entomological Science
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    • v.51 no.1
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    • pp.56-62
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    • 2013
  • We bred new mulberry cultivar through local adaptability test, which was registered as a new cultivar for fruit production. Local adaptability test had been carried out at three places(Suwon, Chuncheon and Jinju) for four years since 2003. This is tetraploidy variety belonging to (Morus Lhou(Ser.) Koidz.) made by colchicine treatment on growing point of winter buds. Daejappong was high yielding in productivity by 3% compared to control cultivar "Chungilppong" for two years.The sugar contents of Daejappong was higher than that of control cultivar 'Chungilppong(Morus alba L.)'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

Phytotoxic Response of Some Fruit Trees to Oxyfluorfen (Oxyfluorfen 처리(處理)에 따른 과수종(果樹種) 및 품종간(品種間)의 약해반응(藥害反應))

  • Cho, Y.W.;Pyon, J.K.;Guh, J.O.
    • Korean Journal of Weed Science
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    • v.7 no.3
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    • pp.337-347
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    • 1987
  • Spary and vapor drift injuries of apple, pear, and peach seedling caused by soil-applied oxyfluorfen were studied in a greenhouse. Bud bursting rate of all fruit trees was reduced by both spray and vapor drifts of oxyfluorfen, but reduction in bud bursting rate of pear and peach was greater than that of apple trees. Reduction in the number of leaves per shoot of apple and peach was greater than that of pear trees. Leaf injury of pear was most severe and occurred earliest, but leaves of peach were least injured, Leaf injuries of pear and apple were caused by both spray and vapor drifts, but leaves of peach was injured largely by vapor drift. Reduction in shoot growth of and pear was greater than that of peach trees. Shoot growth of pear was more rapidly retared compared with apple trees. In the field, oxyfluorfen delayed the time of bud bursting in young apple trees. Oxyfluorfen applied between initiation and completion of bud bursting delayed bud bursting more than earlier application although ultimate number of bursted buds was similar to control. The number of leaves per shoot and total length of shoots were lower than control until 40 days after application of oxyfluorfen, and then were similar to control because of vigorous growth after May.

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Studies on the Cause and Control for Non-Sprouting in the Mulberry Field of Kyung-pook Province (경북지방의 뽕밭에 발생한 발아불량현상의 원인 및 방제에 관한 연구)

  • Yu, Geun-Seop;Kim, Gyu-Rae;Kim, Nak-Sang
    • Journal of Sericultural and Entomological Science
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    • v.28 no.2
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    • pp.1-8
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    • 1986
  • This study was conducted to bring light on inducing factors for non-sprouting occured in the mulberry field of Kyungpook Province in 1983. The results are as follws ; 1. In spring, winter buds were suddenly died during germination, necrosis appeared in pholem and cortex of non-sprouting stem and measles at the bottom of non-sprouting stem. 2. The depth of available of soil was more shallow in the non-sproutiong field than in healthy field. 3. There was no signifioant difference between the healthy field and non-sprouting field in siol PH, the content of organic matter, available phosphorous and exchangeble cations. 4. Available boron content in soil was significantly more in healthy field than in non-sprouting field. 5. Boron content in leaf and bark was significantly lower in the non-sproutiong tree than in the healthy tree. 6. The non-sprouting fields were completely controlled by the application of 6kg/10a borax. 7. Cold tolerance of the mulberry tree was higher in the mulberry tree with boron than in the mulberry tree without boron. 8. Mulberry tree in Kyungpook Province in 1983 may absorb water earlier compared with the average year because of high temperature at the end of March, 1983 and they had been damaged by frost injuary due to the lowest temperature (-6.4$^{\circ}C$) on the grass at the beginning of April 1983. As the above results, non-sprouting bud in the mulberry fields of Kyungpook Province in 1983 had been occured by low temperature and boron deficiency and boron deficiency of mulberry trees accelerared the frost injury.

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Characteristics of Major Diseases causing Eleutherococcus senticosus Max (가시오갈피에서 발생하는 주요 병해충 특성)

  • Lee, Jae-Hong;Jeong, Haet-Nim;Kang, An-Seok;Choi, Kang-Jun
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.3
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    • pp.199-202
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    • 2007
  • This study was carried out to provide pest control information for the cultivation of Eleutherococcus senticosus Max. As a result of investigation of the diseases and insect pests, three pathogens and three insect pests were identified from the field sample, respectively. The identified insects pests were aphids, stinkbugs and Bothrogonia japonica. Occurrenre peaks of aphids were occurred on June and August, and generally young leaves and flower buds were injured mainly. The identified diseases were black ring spot caused by Phoma sp., gray mold caused by Botrytis cinerea and leaf blights caused by Rhizoctonia solani. The black ring spot was the most severe disease and was investigated from the beginning of June, and the incidence of the disease was 30% higher than that on September. As a result of growth test under different temperature conditions on PDA, these pathogens showed the best mycelial growth rate between 25 $^{\circ}$C and 30 $^{\circ}$C, and these results indicate that these diseases occur on hat summer season.

Environmental and Ecological Characteristics Distribution of Natural Growth Region in Rhododendron Brachycarpum (만병초 자생지의 환경생태학적 특성)

  • Lee, Byung-Chul;Shim, Ie-Sung
    • Journal of Environmental Science International
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    • v.20 no.10
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    • pp.1319-1328
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    • 2011
  • Rhododendron brachycarpum is a evergreen broad-leaved shrub and belongs to the Ericaceae family and the Rhododendron genus. It is well known for its beautiful leaves and flowers. There are 11 species of the Rhododendron genus in Korea. It includes 3 species - Rhododendron brachycarpum, Rhododendron aureum Georgi and Rhododendron brachycarpum var. roseum Koidz. They grow naturally over 1,000 meters above sea level of the Baekdu Mountain Range in Korea. These habitats, according to investigations of 9 Rhododendron brachycarpum natural habitats, are mostly located on the slope of mountains facing north at an altitude of 1,200 m to 1,526 m above sea level with angle of inclination from 30 degrees to 45 degrees. Based on the result of vegetation analysis of dominance species in the quadrates, there are Quercus aliena, Quercus mongolica Fisch. ex Ledeb, Abies holophylla in species of upper trees, and so on. Dominant species of woody plants in tree layer are Quercus aliena, Quercus mongolica Fisch. ex Ledeb, Abies holophylla, Betula platyphylla and Veeatrum patulum Loes. fil, Erythronium japonicum, Dryopteris crassirhizoma, Paeonia japonica var. glabra Makino are founded in herbaceous plants. And we can see another result of the investigation that the flowering rates of the plants with the buds are highly ranked mountains such as Mt. Hambaek 68%, Mt. Gyebang 40%, Mt. Yagksu 9%, Mt. Gaein 7% and Mt. Seolag 0%. The results show that there are 24 over 15-year-old Rhododendron brachycarpums in Mt. Odae and are 56 under 15-year-old trees in Mt. Hambaek and are no trees in Mt. Gyebang and are 9 over 30-year-dead trees only in Mt. Taebaeg. Out of found trees, the highest tree is 7 m in height and 0.6 m in diameter. Also this result shows what are the vulnerability factors of the natural habitats. They are as follows: indiscriminate trails in mountains, damages by mountain climbers, uncareful plant collecting, the fierce competitions with other plants such as Acer pseudosieboldianum var. ishidoyanum Uyeki, Quercus aliena, Celastrus orbiculatus and damages by disease and insect, unusual temperature in natural habitats, etc. Rhododendron brachycarpums have high ornamental value and excellent pharmaceutical effect. But the areas of its habitats decrease dramatically. So we need measures to protect and their natural habitats. It is necessary that we conductfurther investigations to designate conservation area for Rhododendron brachycarpums.

Food Quality Comparison of Dried Persimmons (Diospyros kaki THUNB) When using Medicinal Plant Extracts and Food Additives during Drying Process (약용식물 추출물 및 식품첨가제가 곶감 식품학적 품질 비교)

  • Kim, Ki-Ho;Kim, Kyung-Min
    • Current Research on Agriculture and Life Sciences
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    • v.32 no.1
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    • pp.10-17
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    • 2014
  • This study presents an eco-friendly persimmon drying system to satisfy consumer preferences and provide a popular food for both the domestic and international markets. The most effective antimicrobial compounds were developed from a combination of plant extracts (18.18% clove buds, 9.90% cinnamon, 9.09% licorice, 4.55% cnidium, 4.55% seed of grapefruit, and 54.54% apple vinegar). The dried persimmons were evaluated as regards their moisture and sugar content, weight, hardness, and color value. During the drying process, the overall moisture content of the persimmons increased, along with the sugar content. The hardness was almost the same for each region and decreased on an average of 0.5~0.86 after 6 weeks. As regards the chromaticity, ${\Delta}E$ decreased during the drying process, while L-value became darker and a-value showed a dark red color over time.

Flowering, Fruiting, Seed Fall and Seed Viability of Acer ukurunduense in Mt. Jungwang, Gangwondo (강원도 중왕산 지역에서 부게꽃나무의 개화, 결실, 종자낙하량 및 종자활력)

  • Kim, Gab Tae;Kim, Hoi Jin
    • Journal of Korean Society of Forest Science
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    • v.103 no.2
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    • pp.153-158
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    • 2014
  • To examine the natural regeneration in the natural mixed-broadlived forest, flowering, fruiting, seed-fall, and seed viabilities of Acer ukurunduense Trautv. & C.A. Mey. (AU) were investigated in Mt. Jungwang, Gangwon-do, from 2009 to 2013. The flower of AU consisited many male and bisexual flowers on the raceme (unusual panicle) and the arrangement of two sex morphs are differed for each inflorescence. Flowering dates are differed between sex morph in the same inflorescence. Stamens are stop growing and disappeared after pollinated pistil begin to grow in bisexual flowers, and male flowers have vestial pistil. The flowers of AU might be pollinated by Apis mellifera, Thyris fenestrella seoulensis, Cerambycidae sp., Andrenidae sp. and Ctenophora sp., and had some mechanism to prevent feom self pollination. The number of flower buds per inflorescence is 189 on June 8, and that of young samaras per inflorescence is 41.2 on June 21. At last ripened samaras per inflorescence is reduced 33.4 on September 5. Mean annual seedfall of AU was 6,720 ea/ha (ranged 670~17,930). Rates of sound seeds are highest 43.2%, and those of damaged or decayed seeds are 41.8%,. Those of undeveloped and empty seeds are 10.2% and 4.8%, respectively. Successful regeneration of AU might be in masting year and on the gap sites with proper conditions to germinate and grow. To understand the natural regeneration of the species, Genus Acer, further study on the fruiting habit, pre- and post-dispersal seed viability, and annual variation on these factor should be needed.

Culture of Potted $Viola{\times}wittrockiana$ 'Majestic GT Scarlet Shadow' in Various Mixtures of Recycled Perlite and CGF in a Mat Subirrigation System (재사용한 펄라이트 및 다공성 CGF의 혼합배지와 매트저면관수를 이용한 분화$Viola{\times}wittrockiana$ 'Majestic GT Scarlet Shadow'의 재배)

  • Kim Gyeong-Hee;Lee Kang-Mo;Jeong Byoung-Ryong
    • Journal of Bio-Environment Control
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    • v.15 no.3
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    • pp.239-243
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    • 2006
  • This research compared growth and flowering of potted $Viola{\times}wittrockiana$ 'Majestic GT Scarlet Shadow' in various mixtures of recycled perlite and cellular glass foam (CGF). The crop was cultured in a mat subirrigation system. Numbers of leaves, opened flowers, flower buds, and branches, fresh and dry weights of shoot, leaf area, and total chlorophyll concentration were the greatest in plants grown in the medium of used perlite + granular rockwool+ peatmoss (25:50:25, v/v/v). Plant height and length of the longest root were the highest and longest, respectively, in plants grown in the medium of CGF+peatmoss (25:75, v/v). The medium of CGF+peatmoss (25:75, v/v) was better than the medium of used perlite+peatmoss (25:75, v/v) in plant growth. The medium of CGF + granular rockwool (25:75, v/v) was poorer than the medium of recycled perlite + granular rockwool (25:75, v/v).

Enfluence of the Number of the Lower Scaffold Limbs in Slender Spindle Form on the Tree Growth and Development of 'Fuji' Apple Trees ('후지' 사과나무 세장방추형에서 하단측지수가 수체생육에 미치는 영향)

  • Park, Moo-Yong;Yang, Sang-Jin;Park, Jeung-Kwan;Choi, Dong-Geun;Kang, In-Kyu
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.258-263
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    • 2007
  • This study was carried out to investigate the effects of number of the lower scaffold limbs on tree growth, light penetration, fruit yield, and fruit quality in slender spindle in 6-year-old 'Fuji'/M.9 apple trees. With regard to the growth by the numbers of the lower scaffold limbs, the width of the tree was wide and the growth of new shoots was increased when the number of the lower scaffold limbs was five. Compare with other treatments, five lower scaffold limbs showed high light-interception on the upper (150 cm above the ground) and middle (100 cm above the ground) canopy. There was no difference in the total number of the flower buds of the spurs according to the number of scaffold limbs, but the number and cross section area of flower bud on the lower canopy (120 cm above the ground) were increased where the number of the lower scaffold limbs was five. Fruit yield was highest in the treated with five lower scaffold limbs and fruit weight tended to increase where the number of the lower scaffold limbs was five or eight. With regard to fruit quality, there showed no difference in fruit shape index, firmness, acid content, Hunter L and b value according to the location of canopy and the number of the lower scaffold limbs, but the content of soluble solids was highest treated with five lower scaffold limbs. Hunter a value indicating fruit color was found to be highest treated with five lower scaffold limbs whose light interception was highest.