Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Validation of Reference Genes for Quantifying Changes in Physiological Gene Expression in Apple Tree under Cold Stress and Virus Infection

저온과 바이러스 감염에 노출된 사과나무의 생리적 유전자 정량 측정용 유전자들의 발현 분석 및 검증

  • Yoon, Ju-Yeon (Department of Horticultural and Herbal Crop Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Jeong, Jae-Hoon (Department of Fruit Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Seung-Kook (Department of Research Planning and Coordination, Rural Development Administration)
  • 윤주연 (국립원예특작과학원 원예특작환경과) ;
  • 정재훈 (국립원예특작과학원 과수과) ;
  • 최승국 (농촌진흥청 연구정책국 연구운영과)
  • Received : 2020.07.12
  • Accepted : 2020.08.31
  • Published : 2020.09.30
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Abstract

Quantitative reverse transcription PCR is used for gene expression analysis as the accurate and sensitive method. To analyze quantification of gene expression changes in apple plants, 10 housekeeping genes (ACT, CKL, EF-1α, GAPDH, MDH, PDI, THFs, UBC, UBC10, and WD40) were evaluated for their stability of expression during infection by Apple stem grooving virus (ASGV) or in cold-stress apple plant buds. Five reference-gene validation programs were used to establish the order of the most stable genes for ASGV as CKL>THFs>GAPDH>ACT, and the least stable genes WD40CKL>UBC10, and the least stable genes were ACT

정량적역전사중합효소연쇄반응(quantitative reverse transcription PCR)은 정확하고 민감한 방법으로 유전자 발현분석에 사용된다. 사과 식물에서 유전자 발현 변화의 정량적 변화를 분석하기 위해, 사과 잎검은점 바이러스(Apple stem grooving virus, ASGV)에 의한 감염 동안 발현의 안정성에 대해 10개 참조유전자들(ACT, CKL, EF-1α, GAPDH, MDH, PDI, THF, UBC, UBC10 및 WD40)을 평가하였다. AGSV 감염 또는 저온 처리된 사과 식물에서의 10개 참조유전자 발현의 안정은 5가지 프로그램을 사용하여 분석하였다. ASGV 감염 사과식물의 잎 조직에서는 CKL>THFs>GAPDH>ACT 순서로 가장 안정한 유전자로 분석되었으며 WD40CKL>UBC10이고 가장 안정하지 않은 유전자는 ACT

Keywords

References

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