• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.111-118
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• 2015

The objective of this study was to determine the response of rapeseed (Brassica napus) to different planting date in the spring and varieties on growth and flowering characteristics. Eight rapeseed varieties were sowed at 10 day interval from 1st to 31th of March at Muan and Jeju in Korea. Significant planting dates and rapeseed varieties effects for growth, start of flowering and duration. As the planting date was being delayed, plant length and flower number were decreased, but branch number was increased. And, start of flowering date was retarded and flowering duration decreased with later planting date. Days from planting to flowering was shortened as seeding date was delayed and shortening degree was similar between experimental locations, Muan and Jeju. The days to flowering for rapeseed about 73~94 days for 1st March and then decreased to 57~71 days for the 31th March of planting date. 'Tammiyuchae' and 'Mokpo 111' seeded on each planting date come into blossom more earlier about 10 days as compared to 'Tamlayuchae' and 'Naehanyuchae'. The duration of flowering for the B. napus varieties was shortened as planting date was delayed. The results revealed that flowering characteristics of rapeseed can be greatly enhanced by planting as early as possible, and early flowering varieties i.e. 'Tammiyuchae' and 'Spring' were the most suitable varieties among the tested varieties for planting in the spring.

• Journal of Environmental Science International
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• v.7 no.2
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• pp.123-132
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• 1998

The experiment was performed to elucidate the effect of simulated acrid rain on growth of the seven crops(caucus carota vu sativa DC. , Fagopyrum esculenkm Moench, Brassica cmpespis subsp. napus var. pekinensis Makino , Raphuus satlws vu. hortensis for. acnMormis Makino, Brassica dbogjabra Bailey, Caphslcum mum L., and Perilla frutescens Britton). The pH levels of simulated acid rain ranged pH 3.1, 3.6, 4.1, 4.6, 5.1 and 5.6. The germination 10 each crop was influenced from stimulated acrid rain except buckwheat and kale. A general decrease of growth in crops was observed with Increasing pH concentrations. The pattern of soluble protein was observed a tendency to decrease from acidic pH eradlents. According to acidity, total DNA contents of each crop was showed a definite reduction. In conclusion, plant growth was stimulated decreasln and the chanties of total Protein Patterns and BNA contents extracted from leaves trended with stimulated acrid rain was showed seriously.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.2
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• pp.49-57
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• 1980

A $F_1$ hybrid out of 143 crosses of rape (Brassica napus) using cytoplasmic-genetic male sterility yielded 5.18 tons per hectare. European and native varieties used, were found out to have 78% and 58% of genes restoring fertility, respectively. The $F_1$ hybrids of three way cross using non-isogenic maintainer showed higher heteroses than those of double cross. To produce F 1 hybrids of three way cross, three stages of crossing operations are needed and seeds produced from about 60 hectares can be covered to 60, 000 hectares.

• Molecules and Cells
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• v.22 no.3
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• pp.300-307
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• 2006

Brassica rapa ssp. pekinensis (Chinese cabbage) is an economically important crop and a model plant for studies on polyploidization and phenotypic evolution. To gain an insight into the structure of the B. rapa genome we analyzed 12,017 BAC-end sequences for the presence of transposable elements (TEs), SSRs, centromeric satellite repeats and genes, and similarity to the closely related genome of Arabidopsis thaliana. TEs were estimated to occupy 14% of the genome, with 12.3% of the genome represented by retrotransposons. It was estimated that the B. rapa genome contains 43,000 genes, 1.6 times greater than the genome of A. thaliana. A number of centromeric satellite sequences, representing variations of a 176-bp consensus sequence, were identified. This sequence has undergone rapid evolution within the B. rapa genome and has diverged among the related species of Brassicaceae. A study of SSRs demonstrated a non-random distribution with a greater abundance within predicted intergenic regions. Our results provide an initial characterization of the genome of B. rapa and provide the basis for detailed analysis through whole-genome sequencing.

• Research in Plant Disease
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• v.25 no.2
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• pp.98-101
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• 2019

Pak choi (or a Bok choy; Brassica rapa subsp. chinensis) is a popular brassicaceous vegetable worldwide. In January 2019, a downy mildew symptom on pak choi was found at a farm located in Yongin, Korea. Based on morphological characteristics and molecular phylogenetic inference, the pathogenic oomycete was identified as Hyaloperonospora brassicae. To our knowledge, this is the first report of downy mildew disease occurring on pak choi in Korea. Considering the increasing demand for this crop, this pathogen would be a potentially new threat to the cultivation of pak choi in Korea.

• BMB Reports
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• v.40 no.2
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• pp.247-260
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• 2007

Cinnamate 4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which synthesizes numerous secondary metabolites to participate in development and adaption. Two C4H isoforms, the 2192-bp BnC4H-1 and 2108-bp BnC4H-2, were cloned from oilseed rape (Brassica napus). They both have two introns and a 1518-bp open reading frame encoding a 505-amino-acid polypeptide. BnC4H-1 is 57.73 kDa with an isoelectric point of 9.11, while 57.75 kDa and 9.13 for BnC4H-2. They share only 80.6% identities on nucleotide level but 96.6% identities and 98.4% positives on protein level. Showing highest homologies to Arabidopsis thaliana C4H, they possess a conserved p450 domain and all P450-featured motifs, and are identical to typical C4Hs at substrate-recognition sites and active site residues. They are most probably associated with endoplasmic reticulum by one or both of the N- and C-terminal transmembrane helices. Phosphorylation may be a necessary post-translational modification. Their secondary structures are dominated by alpha helices and random coils. Most helices locate in the central region, while extended strands mainly distribute before and after this region. Southern blot indicated about 9 or more C4H paralogs in B. napus. In hypocotyl, cotyledon, stem, flower, bud, young- and middle-stage seed, they are co-dominantly expressed. In root and old seed, BnC4H-2 is dominant over BnC4H-1, with a reverse trend in leaf and pericarp. Paralogous C4H numbers in Brassicaceae genomes and possible roles of conserved motifs in 5' UTR and the 2nd intron are discussed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.237-241
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• 2006

This experiment was carried out comparison with haploid plants productivity by microspore culture among domestic cultivars of Brassica napus L. Isolated microspore from flower buds were cultured on NLN medium supplemented with 13% sucrose, $0.05mg/{\ell}$ BA and $0.5mg/{\ell}$ NAA. Genotype was important factor in haploid embryo productivity 'Tamlayuchae' showed the highest haploid embryo production frequency (176 embryos formed from 1 flower bud). But, 'Hallayuchae' and 'Youngsanyuchae' were not generated embryo even cell division. When suspension culture on NLN liquid medium at 100 rpm, embryos were developed multilobe abnormal embryo cluster. Multilobe abnormal embryos on MS medium basal solid medium were regenerated multiple shoots. Regenerated haploid plant with well developed shoots and roots on MS basal medium were successfully transferred to pots.

• The Plant Pathology Journal
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• v.37 no.1
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• pp.47-56
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• 2021

Plants protect against viruses through passive and active resistance mechanisms, and in most cases characterized thus far, natural recessive resistance to potyviruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimolecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and translation initiation factors to reveal the working of key amino acids.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.332-340
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• 2016

Seed size traits are controlled by multiple genes in crops and determine grain yield, quality and appearance. However, the molecular mechanisms controlling the size of plant seeds remain unclear. We performed functional analysis of BrPATL4 encoding Sec14-like protein to determine the genetic architecture of seed size, shape and their association analyses. We used 60 $T_3$ transgenic rice lines to evaluate seed length, seed width and seed height as seed size traits, and the ratios of these values as seed shape traits. Pleiotropic effects on general architecture included small seed size, erect panicles, decreased grain weight, reduced plant height and increased sterility, which are common to other mutants deficient in gibberellic acid (GA) biosynthesis. To test whether BrPATL4 overexpression is deleterious for GA signal transduction, we compared the relative expression of GA related gene and the growth rate of second leaf sheath supplied with exogenous $GA_3$. Overexpression of BrPATL4 did not affect GA biosynthesis or signaling pathway, with the same response shown under GA treatment compared to the wild type. However, the causal genes for the small seed phenotype (D1, SRS1, and SRS5) and the erection of panicles showed significantly decreased levels in mRNA accumulation compared to the wild type. These results suggest that the overexpression of BrPATL4 can control seed size through the suppression of those genes related to seed size regulation. Although the molecular function of BrPATL4 is not clear for small seed and erect panicles of BrPALT4 overexpression line, this study provides some clues about the genetic engineering of rice seed architecture.

• Journal of Environmental Science International
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• v.33 no.1
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• pp.27-41
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• 2024

In this study, we evaluated productivity, soil physiochemical properties, and soil microbial characteristics in Kimchi cabbage(Brassica rapa subsp. pekinensis) cultivation within a highland environment during summer. Specifically, we examined the effect of different cropping systems, namely monoculture and rotation with soybean, over an 8-year cropping period. The results of our investigation revealed that significant differences were absent in terms of yield and soil physiochemical properties between the two cropping systems. However, microbial characteristics exhibited distinctive patterns. Bacterial diversity was significantly higher in the rotation system that in the monoculture, whereas fungal diversity demonstrated a preference for rotation although the result was not significant. Our findings identified the presence of Bradyrhizobium stylosanthis, a nitrogen-fixation symbiont, as an indicator ASV (amplicon sequence variant) in the rotation system, where it displayed significantly higher abundances. These observations suggest a potential positive effect of the rotation system on nitrogen fixation. Notably, throughout the cultivation period, both cropping systems did not exhibit critical disease incidences. However, Fusarium oxysporum, a well-known pathogen responsible for inducing fusarium wilt disease in Kimchi cabbage, was detected with significantly higher abundance in the monoculture system. This finding raises concerns about the potential risk associated with Kimchi cabbage cultivation in a long-term monoculture system.