• 한국임상수의학회지
• /
• 제33권1호
• /
• pp.70-73
• /
• 2016

Necrotizing meningoencephalitis (NME) is a unique idiopathic nonsuppurative inflammatory disease of central nervous system in small-sized breed dogs. A 9-year-old intact male Shih-Tzu dog with anorexia, vomiting, salivation and intermittent seizures was submitted to the Jeju National University for diagnosis. Grossly, there were no obvious lesions in the brain, except dilatation of most blood vessels in meninges. Histopathologically, brain revealed severe multifocal nonsuppurative inflammation in perivascular area of meninges and cerebral cortex. Some areas of cerebral parenchyma were replaced with lots of macrophages contained periodic acid-Schiff positive materials. Many new-formed blood vessels were observed around the necrotic regions using Gomori reticulum stain. Immunohistochemistry and reverse transcription-polymerase chain reaction were negative for toxoplasmosis and canine distemper virus. Based on the gross, histopathologic features and antigen detection methods, this case was diagnosed as NME. Here we reported the NME in relatively uncommon breed, Shih-Tzu dog, than other small breed dogs.

• 한국임상수의학회지
• /
• 제24권2호
• /
• pp.177-181
• /
• 2007

In the present study, a total of 547 half milk samples were collected from 274 dairy goats to perform somatic cell counts (SCC) and California mastitis test (CMT). Milk smear was stained with Pyronin Y-Methyl Green stain were classified into either epithelial or blood cells, etc. Of the 547 halves the percentage of CMT negative milk samples were 86%. Among these, 58.2% were CMT negative with SCC<500,000/ml, while 27.8% were CMT negative with SCC>500,000 ml. As expected, CMT score increased with the increase of SCC. The number of epithelial cells decreased with the increasing number of somatic cells, while the opposite was observed with the number of blood cells. These results indicate that the critical point in milk quality & CMT should be considered on the false (pseudo-SC) SCC in dairy goat.

• 한국염색가공학회:학술대회논문집
• /
• 한국염색가공학회 2008년도 제38차 학술발표대회
• /
• pp.181-183
• /
• 2008

Four different subtilisins of protease were investigated to see their effects on the cleaning activity. The cleaning solution was formulated with various non-ionic surfactants and other additives such as propylene glycol, triethanolamine, pH balancer etc. to evaluate their effect on enzyme activity as well. Evaluation of formulated cleaning solution was carried under K0120 using pre-soiled textiles from EMPA. The results showed that the cleaning activity on soil removal was not severly influenced by surfactant but the enzyme mostly. In addition, the activity of enzymes was not much affected by the type of surfactants as long as the surfactants were non-ionic. Liquinase among the four enzymes used in this study showed the best performance on soil removal, especially blood stain.

• Parasites, Hosts and Diseases
• /
• 제33권4호
• /
• pp.391-394
• /
• 1995

말라리아의 진단 방법으로 흔히 사용되고 있는 Giemsa 염색법과 형광염색법중 Acridine orange(AO) 염색법을 비교하였다. 말라리아 환자의 혈액을 채취하여 Giemsa와 AO로 염색하여 각각 광학현미경 및 형광현미경으로 관찰하였다. AO 염색법은 Giemsa 염색법에 비해 저배율에서도 쉽게 말라리아 원충을 찾을 수 있어 빠르고 정확한 말라리아의 진단을 위해 Acridineorange 염색법이 더 효과적이라고 생각된다.

• 한국치위생학회지
• /
• 제7권2호
• /
• pp.189-196
• /
• 2007

A study of the J health college dept of dental hygiene practice vistant a total of 35 supragingival calculus and subgingival calculus picking SEM observation and bacteria identification of the result are followings. 1. As observed by dental calculus SEM, the surface roughness appeared as peaks, valleys, and pits. 2. About bacteteria morphology blood agar plate small green zone partial hemolysis colony streptococcus observation 3. Isolated colony gram stain gram are positive display 4. Supragingival calculus at Lactococcus lactis spp. Leuconostoc spp. Streptococcus mitis, Aerococcus viridans bacteria 1, 3, 3, 16 species detection 5. Subgingival calculus at Aerococcus viridans, Leuconostoc spp. bacteria 5, 1 species detection.

• Imaging Science in Dentistry
• /
• 제41권3호
• /
• pp.123-128
• /
• 2011

Arteriovenous malformations are extremely rare conditions in that can result from abnormalities in the structure of blood vessels, which may be potentially fatal. A 30-year-old female patient visited our hospital with a complaint of swelling on the right maxillary posterior gingiva along with the large port-wine stain on right side of face. On clinical examination, the swelling was compressible and pulsatile. Radiographic examination revealed a lytic lesion of maxilla. Diagnostic angiography revealed a high-flow arteriovenous malformation of maxilla which was treated by selective transarterial embolization of maxillary artery using polyvinyl alcohol particles.

• Parasites, Hosts and Diseases
• /
• 제54권4호
• /
• pp.393-397
• /
• 2016

Low parasitemic condition in malaria remains a diagnostic challenge; as the available diagnostic methods failed to detect. Currently, hemozoin (Hz) pigment is gaining attention in the diagnosis of malaria. The major drawback is ease of detection of Hz in routine practice. A pilot study was conducted to evaluate the role of Hz pigment and to compare the performance of quantitative buffy coat assay (QBC) and PCR in such conditions. Clinically suspected cases of malaria were examined by both Giemsa stain and immunochromatographic test (ICT). Samples positive by ICT and negative by Giemsa stain were further examined by nested PCR targeting 18S rRNA and QBC for the presence of malaria parasites and pigments. Thirty blood samples fulfilled the inclusion criteria out of which 23 were Plasmodium vivax (Pv), 4 Plasmodium falciparum (Pf), and 3 mixed (Pv and Pf) by immunochromatographic test. Twenty-one out of 30 (70%) were positive by nested PCR in comparison to 25/30 (83%) by QBC. Samples containing both malaria parasites and Hz pigment by QBC completely showed concordance with the PCR result. However, 61% of total samples containing only Hz pigment were observed positive by PCR. Hz pigment remains an important tool for malaria diagnosis. Identification of leukocytes containing pigments by QBC not only indicates recent malarial infections but also puts light on severity of the disease. QBC assay is a rapid, highly sensitive, and cost-effective method to detect malaria parasites and Hz pigment especially in low parasitemic conditions.

• Archives of Plastic Surgery
• /
• 제36권3호
• /
• pp.254-261
• /
• 2009

Purpose: The objective of this study was to evaluate the heparin effect for a viability of random - pattern dorsal flap in hairless mouse. Methods: A caudally - based random dorsal flap, measuring $1.5{\times}5cm$, was designed and heparin was applied topically after microneeding. Twenty five male hairless mice were randomly divided into control (Group1, n=5); received only microneedling (Group 2, n=5), only heparin(Group3, n=5), microneedling with saline(Group 4, n=5), and microneedling with heparin group(group5,n=5) to the flap during 7 days. The number of the capillaries were compared between the experimental groups and control group with respect to neovascularization after heparin application using imaging analysis program under hematoxylin - eosin stain. The capillary blood flow was measured by laser Doppler flowmetry. After seven days each animal was evaluated for the percentage area of the flap survival. Mann - Whitnety U test and Kruskal - Wallis statistical analysis of survival relationships was performed. Results: It can be observed increased number of the blood vessels in the experimental groups however it was not statistically significant. Blood flow of the haparin with microneedling group maintained higher than other experimental groups. Treated microneeding and heparin mice were significantly better flap viability than in controls (flap survival 67% and $54.4mm^2$ respectively; p<.01). Positive correlation was shown between flap survival rate and laser Doppler flux value only at first day after surgery. Conclusion: Heparin has a beneficial effect on capillary flow and improve peripheral circulatory disturbances in random pattern flaps.

• Journal of Korean Neurosurgical Society
• /
• 제29권10호
• /
• pp.1289-1295
• /
• 2000

Purpose : Recent evidence suggests a possible role for leukocytes in brain injury following ischemia and reperfusion. This study examined the temporal profile of ischemic tissue damage and leukocyte response after transient middle cerebral artery occlusion(MCAO) with reperfusion in the mouse. Methods : Focal cerebral ischemia was made by temporary occluding of the stem of the proximal MCA. Two groups of the mouse were investigated : (1) sham operation(n＝10), and (2)those having the arterial occlusion released after 90 minute(n＝20). By 4 hours(n＝10) and 24 hours(n＝10) after the onset of ischemia-reperfusion, fluorescein videoimages were under-taken in the pial venules of the mouse using a closed cranial window technique. Rhodamine 6G was administered as a $80-100{\mu}l/min$ i.v. loading dose and a $30-40{\mu}l/min$ i.v. maintenance dose in saline to selectively label circulating leukocytes. Neuropathologic evaluation for brain injury was accomplished using the histochemical stain 2,3,5-triphen-yltetrazolium chloride(TTC) and hematoxylin and eosin(H & E) stain. Results : The mean number of adherent leukocytes to cerebral venules in the 90 minutes MCAO and 24 hours reperfusion group were $306{\pm}24$ compared with $72{\pm}8$ in the sham operation group. In the TTC staining method, the cortical infarct affecting 34.8% of hemispheric volume were created in all of animals (n＝10) undergoing 90 minute MCAO with 24 hours reperfusion, but the infarcted area were not found in the other(sham operation and 90 minute MCAO with 4 hours reperfusion)groups. In the H & E stain, the brain tissue following 90 minute MCAO with 4 hours reperfusion revealed only a pyknosis of the nuclei with shrunken cytoplasm, but infiltrated leukocytes were not observed. After 24 hours of reperfusion, a many leukocytes were infiltrated within parenchyma and blood vessles. Conclusions : These findings demonstrate the feasiblity of continous in vivo monitoring of leukocyte adherence in cerebral venules and suggest that reperfusion induced leukocyte adherence to venular endothelium may contribute to tissue injury following focal cerebral ischemia.

• 대한치과교정학회지
• /
• 제28권3호
• /
• pp.419-429
• /
• 1998

본 실험은 유성견에서 인위적인 정출 시 조직학적, 임상학적인 반응과 시간경과에 따른 치아 및 치아 주위조직의 회복을 연구하기 위해 생후 평균 10 개월 웅성 잡종 유성견 하악 소구치를 대상으로 Energy chain을 이용한 정출 실험으로, 실험 1 군은 힘을 작용 시킨 후 일 주일만에 희생시키고 실험 2 군은 정출력을 가한 후 일 주일 후, 정출력이 제거되고 3 주간의 회복 기간 경과 후에 희생시켰다. HE 및 Masson's Trichrome 염색법을 이용하여 치아와 주위 조직의 반응을 치경부, 치근 중앙부, 치근단부, 치수부로 분류하여 광학 현미경하에서 관찰하여 다음과 같은 결과를 얻었다. 1. 힘을 가한 후 일 주일 후, 모든 부위에서 치주인대의 주행방향이 치아를 향하여 사선 방향을 나타내 주었으며 치주인대의 간격이 전반적으로 증가되었다. 또 부분적인 치주 인대의 파열이 치근단부에서 뚜렷하게 관찰되었다. MT 소견에서는 대조군에 비해 치조능 부위에서 미성숙 골의 형성을 보여주었다. 주위 치조골에서는 편측으로 활발한 골조직의 형성이 관찰되었다. 치수에서는 치근단 부위에서 많은 혈관의 파열을 보여 주었고 공포의 형성과 충혈을 나타냈다. 2. 힘을 제거 된 후3주간의 보정을 한 군의 모든 치주인대의 주행 방향과 간격이 대조군과 같이 회복되었으며 밀도는 치밀하게 나타났다. MT소견에서 1군에서 형성된 치조능의 미성숙 골이 석회화가 이루어짐을 보여주었고 치수의 공포와 충혈의 감소를 보여주었다. 수복과정에서의 치수의 섬유화가 더욱 진행되었다. 이상의 결과를 종합해 볼 때, 3주간의 회복 기간 중에도 치주 인대 부위에서는 대조군과 유사한 조직 소견을 보여 상당한 회복능력을 보였지만 치수에서는 섬유화가 진행됨을 보여 더욱 긴 회복 기간이 필요함을 보여주었다.