Journal of Korean Neurosurgical Society
- Volume 29 Issue 10
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- Pages.1289-1295
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- 2000
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- 2005-3711(pISSN)
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- 1598-7876(eISSN)
Leukocyte-Endothelial Cell Adhesion Induced by Ischemia and Reperfusion Observed with in vivo Videomicroscopy
허혈-재관류에 의해 유도된 백혈구-혈관내피세포 유착에 대한 Videomicroscopy 영상소견
- Lee, Young Bae (Department of Neurosurgery, College of Medicine, Dong Guk University) ;
- Kang, Han Sug (Department of Neurosurgery, College of Medicine, Dong Guk University) ;
- Park, Shin Byung (Department of Neurosurgery, College of Medicine, Dong Guk University)
- Received : 2000.05.15
- Accepted : 2000.07.18
- Published : 2000.10.28
Abstract
Purpose : Recent evidence suggests a possible role for leukocytes in brain injury following ischemia and reperfusion. This study examined the temporal profile of ischemic tissue damage and leukocyte response after transient middle cerebral artery occlusion(MCAO) with reperfusion in the mouse. Methods : Focal cerebral ischemia was made by temporary occluding of the stem of the proximal MCA. Two groups of the mouse were investigated : (1) sham operation(n=10), and (2)those having the arterial occlusion released after 90 minute(n=20). By 4 hours(n=10) and 24 hours(n=10) after the onset of ischemia-reperfusion, fluorescein videoimages were under-taken in the pial venules of the mouse using a closed cranial window technique. Rhodamine 6G was administered as a
Keywords
- Transient middle cerebral artery occlusion;
- Ischemia;
- Reperfusion;
- Leukocytes;
- Videomicroscopy imaging