• Title/Summary/Keyword: Biosensors

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Immunosensors for Food Safety: Current Trends and Future Perspectives

  • Daliri, Frank;Aboagye, Agnes Achiaa;Kyei-Baffour, Vincent;Elahi, Fazle;Chelliah, Ramachandran;Daliri, Eric Banan-Mwine
    • Journal of Food Hygiene and Safety
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    • v.34 no.6
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    • pp.509-518
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    • 2019
  • To monitor the levels of antimicrobials, allergens, pathogens and other contaminants in foods meant for human consumption, it is imperative to have quick, accurate and low-cost tests. Advanced techniques (e.g. label-free biosensor assays) have been developed over the past 10-15 years to solve some of these problems. As biosensors, immunosensors can provide real-time measurements, a high degree of automation, and improved throughput and sensitivity. By comparison with conventional methods, immunosensors are less expensive, less sophisticated physicochemical instruments that require less time for analysis while also being more user-friendly. In this review, we discuss our current knowledge about immunosensors, their strengths and weaknesses, as well as the future of these biosensors in food safety.

The Classification and Investigation of Smart Textile Sensors for Wearable Vital Signs Monitoring (웨어러블 생체신호 모니터링을 위한 스마트텍스타일센서의 분류 및 고찰)

  • Jang, Eunji;Cho, Gilsoo
    • Fashion & Textile Research Journal
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    • v.21 no.6
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    • pp.697-707
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    • 2019
  • This review paper deals with materials, classification, and a current article investigation on smart textile sensors for wearable vital signs monitoring (WVSM). Smart textile sensors can lose electrical conductivity during vital signs monitoring when applying them to clothing. Because they should have to endure severe conditions (bending, folding, and distortion) when wearing. Imparting electrical conductivity for application is a critical consideration when manufacturing smart textile sensors. Smart textile sensors fabricate by utilizing electro-conductive materials such as metals, allotrope of carbon, and intrinsically conductive polymers (ICPs). It classifies as performance level, fabric structure, intrinsic/extrinsic modification, and sensing mechanism. The classification of smart textile sensors by sensing mechanism includes pressure/force sensors, strain sensors, electrodes, optical sensors, biosensors, and temperature/humidity sensors. In the previous study, pressure/force sensors perform well despite the small capacitance changes of 1-2 pF. Strain sensors work reliably at 1 ㏀/cm or lower. Electrodes require an electrical resistance of less than 10 Ω/cm. Optical sensors using plastic optical fibers (POF) coupled with light sources need light in-coupling efficiency values that are over 40%. Biosensors can quantify by wicking rate and/or colorimetry as the reactivity between the bioreceptor and transducer. Temperature/humidity sensors require actuating triggers that show the flap opening of shape memory polymer or with a color-changing time of thermochromic pigment lower than 17 seconds.

Improvement in Sensitivity of Electrochemical Glucose Biosensor Based on CuO/Au@MWCNTs Nanocomposites (CuO/Au@MWCNTs 나노복합재 기반 전기화학적 포도당 바이오센서의 민감도 개선)

  • Park, Mi-Seon;Bae, Tae-Sung;Lee, Young-Seak
    • Applied Chemistry for Engineering
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    • v.27 no.2
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    • pp.145-152
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    • 2016
  • In this study, CuO was introduced on MWCNTs dispersed with Au nanoparticles to improve the glucose sensing capability of electrochemical biosensors. Nano-cluster shaped CuO was synthesized due to the presence of Au nanoparticle, which affects glucose sensing performance. The biosensor featuring CuO/Au@MWCNTs nanocomposite as an electrode material when 0.1 mole of CuO was synthesized showed the highest sensitivity of $504.1{\mu}A\;mM^{-1}cm^{-2}$, which is 4 times better than that of MWCNTs based biosensors. In addition, it shows a wider linear range from 0 to 10 mM and lower limit of detection (LOD) of 0.008 mM. These results demonstrate that CuO/Au@MWCNTs nanocomposite sensors are superior to other CuO based biosensors which are attributed that the nano-cluster shaped CuO is favorable for the electrochemical reaction with glucose molecules.

A Study on the Detection Characteristics in Glucose and Fabrication of Bi-Enzyme Electrode using Electrochemical Method (전기화학적 방법을 이용한 다중 효소 전극 제작 및 글루코스 검출 특성에 관한 연구)

  • Han, Kyoung Ho;Shin, In Seong;Yoon, Do-Young
    • Journal of the Korean Electrochemical Society
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    • v.23 no.3
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    • pp.66-72
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    • 2020
  • In this study, the development of biosensors capable of bi-enzyme reactions by including Horseradish peroxidase and glucose oxidase was carried out for detection of glucose. The sensors were manufactured using electro deposition method to reduce production time, and screen printed electrodes (SPE) were used to produce economical sensors. To check the bienzyme effect, the sensor was compared and analyzed with single enzyme biosensor. The characteristics of the sensor were evaluated using scanning electron microscopy(SEM), cyclic voltammetry(CV), electrochemical impedance spectroscopy(EIS), chronoamperometry(CA), and flow injection analysis(FIA). Analysis results from SEM, CV and EIS confirmed that the enzymes are well fixed to the electrode surface. In addition, it was confirmed that bi-enzyme biosensors manufactured from the CA method improved signal performance by 200% compared to single enzyme biosensors. From this results, we were able to explain that HRP and GOD react catalyzed to each other. And the results of FIA showed that the intensity of each current signal was constant when the same concentration of glucose was injected four times. In addition, by analyzing the intensity of current signals for glucose concentrations, the biosensors manufactured in this study showed excellent trends in signal sensitivity, reproducibility and stability.

The Theory and Application or Piezoelectric Quartz Crystal Microbalance[PZ QCM] for Biosensor (압전 수정 결정 미량 천평[PZ QCM] 바이오센서의 원리와 응용)

  • 김의락
    • KSBB Journal
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    • v.18 no.2
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    • pp.79-89
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    • 2003
  • This article contains an overview of acoustic wave devices, the theory and application of piezoelectric quartz crystal microbalances(PZ QCM), clinical analysis, gas phase detection, DNA biosensors, drug analysis, food microbial analysis and environmental analysis.