• Title/Summary/Keyword: Bioluminescence

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Continuous Water Toxicity Monitoring Using Immobilized Photobacterium phosphoreum

  • Kim, Se-Kwon;Lee, Baek-Seok;Lee, Jeong-Gun;Seo, Hyung-Joon;Kim, Eun-Ki
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.2
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    • pp.147-150
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    • 2003
  • Water toxicity monitoring based on the continuous cultivation of Photobacterium phorphoreum is presented. Normally, after 10 days of operation, a dark variant, which emits no light, appears and dominates the population, resulting in a rapid decrease in bioluminescence. Therefore, to overcome this problem, a fluidized-bed reactor is used in which alginate-immobilized cells are grown and leaking cells are continuously released into the effluent Experimental results revealed that the dominance of dark variants was suppressed inside the immobilized beads, thereby mitigating the rapid loss of bioluminescence. Plus, a high dilution rate (1.2 h$\^$-1/) prevented the occurrence of other microbial contamination in the reactor The concentration and bioluminescence of the released cells were sufficient to measure the water toxicity for more than 4 weeks.

Comparative Studies on the Acute Toxicities of Whole Solids and Solids Aqueous Extracts Based on the Inhibition of Bacterial Bioluminescence Production

  • Kong, In-Chul
    • Environmental Engineering Research
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    • v.16 no.1
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    • pp.41-45
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    • 2011
  • The aim of this investigation was to demonstrate a rapid bioluminescence bioassay for comparison of the toxicity of whole solids and the aqueous extracts of various environmental solid samples. With regard to the toxicities, those for the soil extracts were mostly found to be lower than those of whole soils, which may have been caused by un-extracted pollutants or dilution during the extraction process. Solid samples from dam-reservoir sediments and municipal refuses were also tested. The toxicities of the solid extracts (0-34%; refuses and sediments) were much lower than those of the whole solids (13-91%). The bioluminescence inhibition test indicated that the harmful effects of the contaminated solids samples were greater than those of the solid extracts.

Diagnostic test for bovine mastitis by the determination of ATP based on firefly bioluminescence (Bioluminescence 반응에 의한 ATP 측정을 이용한 젖소 유방염 진단에 관한 연구)

  • Kim, Tae-jong;Kim, Jong-bae;Lee, Seoung-bae;Jeon, Young-soo
    • Korean Journal of Veterinary Research
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    • v.29 no.3
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    • pp.383-391
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    • 1989
  • This study was carried out to diagnostic test for bovine mastitis by the determination of adenosine triphosphate (ATP) based on firefly bioluminescence. The results obtained are follow; 1. The infection rate of bovine mastitis investigated with 521 cows in 47 dairy farms were found to be 3.6% of clinical form and 44.1% of subclinical form according to the degree of infection. 2. The light yield produced in firefly bioluminescence system was proportional to the concentration of ATP giving stright line within the range of 100PM~1uM. 3. When the number of somatic cell in milk was determined by the ATP assay and compared with three conventional methods such Fossomatic, California mastatic test (CMT), and rolling ball viscometer (RBV), it was shown that r=0.92 for Fossomatic, 0.63 for CMT and 0.7 for RBV. 4. The microorganisms causing mastitis were isolated Staphylococcus sp. (53.3%), Streptococcus sp. (17.9%), Micrococcus sp. (13.5%), Gram negative bacilli (6.3%), Gram positive bacilli (5.5%) and Yeast-like fungi (5.4%). 5. The endogeneous ATP levels of bacteria in a raw milk determined by the firefly bioluminescence system and compared with the results of the conventional methods. The correlation was 0.88 for raw milk.

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Application of the Recombinant Bioluminescence Bacterium on the Toxicity Assessment of the Sole Chemicals and Soil Samples (유전자 재조합 생물 발광 균주를 이용한 순수 오염물과 토양시료의 독성도 평가)

  • Kong, In-Chul;Kim, Jin-Yeong;Lee, Sun-Hee;Ko, Kyung-Seok
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.2
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    • pp.136-142
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    • 2012
  • Various factors affecting on the bioassay were investigated. Experiments with a low mixture ratio (cell to toxicant solution) of 0.5 : 9.5 (v/v) produced observable bioluminescence intensity for assay. Both sodium lactate and potassium nitrate stimulate bioluminescence activity; 2.6~4.0 times of control. Distilled water and MSM, which gave non significant effects on the bioluminescence activity, were determined as proper diluent or extract solutions. A wide range of toxic responses of metals and organics were observed. In general, organics were much less sensitive than metals. Samples collected from eleven sites showed the bioluminescence activity ranging from 29 to 111% of the control. Significant correlation between toxicity and total metal contents was not observed, but the toxicity of two groups, sorted based on the contaminated arsenic concentration in soils, was 44% and 20%, showing considerable differences.

Application of ATP bioluminescence assay for effect assessment of hygiene visiting education on children's foodservice facilities in the local small town (ATP bioluminescence assay를 이용한 경북 일부 어린이 급식시설에 대한 위생방문교육의 효과 평가)

  • Pak, Hye-Jin;Cheigh, Chan-Ick
    • Korean Journal of Food Science and Technology
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    • v.53 no.4
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    • pp.501-508
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    • 2021
  • The purpose of this study was to evaluate the application of ATP bioluminescence assay for effect assessment of hygiene visiting education on children's foodservice facilities in the local small town. The sanitary inspection results of the hygiene visiting education showed significant improvement in all items except 'To regularly disinfect cooking facilities, storage rooms, etc.' ATP bioluminescence analysis was performed on cooking utensils to evaluate the educational effect, and the results showed a correlation with the effect in most items. In the case of knives and cutting boards, all facilities showed a value of 20 RLU/cm2 or less after the educational support, and in particular, it was approximately 10 RLU/cm2 or less in national/public, corporation and private facilities. Correlation analysis between the post-education sanitary inspection results and ATP values for three items related to knives and cutting boards showed that they have a negative and high correlations.

Application of ATP Bioluminescence Assay for Measurement of Microbial Contamination in Fresh-cut Produce Processing Lines (신선편이 농식품 생산라인의 환경미생물 오염도 측정을 위한 ATP 검사법의 이용)

  • Choi, Ji-Weon;Lee, Hye-Eun;Kim, Chang-Kug;Kim, Won-Bae;Kim, Ji-Kang
    • Food Science and Preservation
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    • v.19 no.1
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    • pp.62-66
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    • 2012
  • With the rapid growth of fresh-cut produce market, the South Korean fresh-cut industry is facing the challenge of ensuring food safety. As the estimation of the microbial numbers in fresh-cut produce processing lines (tools, and equipment) using the conventional microbiological techniques takes days, so there is a need for faster and easier monitoring methods. This study was conducted to investigate the use of ATP bioluminescence assay to measure the degree of microbial contamination from three actual fresh-cut processing lines. The samples collected from frech-cut vegetables, and fresh-cut fruits processing plants were tested for the estimation of the bacterial number, using the ATP bioluminescence and microbiological methods. The result of former was transferred to log RLU/100 $cm^2$, and that of the latter was transferred to log CFU/100 $cm^2$. A positive linear correlation between the ATP bioluminescence assay value and aerobic-plate count was found for fresh-cut processing lines, with a correlation coefficient of 0.8772 (n=50). The results of this study indicate that ATP bioluminescence assay can be used to monitor microbial contamination in fresh-cut produce processing plants, and can help improve the hygiene therein.

Changes in Bioluminescence of Omphalotus japonicus Mycelia under Environmental Stress Conditions (환경 스트레스 조건에 따른 화경버섯 균사의 생물발광 변화)

  • Park, Mi-Jeong;Lee, Hyorim;Ryoo, Rhim
    • The Korean Journal of Mycology
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    • v.48 no.4
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    • pp.381-388
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    • 2020
  • Bioluminescence refers to the production and emission of light in living organisms. This phenomenon arises from luciferase-catalyzed oxidation reaction of luciferin. Bioluminescence is widely observed in marine vertebrates and invertebrates, as well as in some microorganisms and fungi. To date, approximately 80 species of fungi have been reported to be luminous. One such example is Omphalotus japonicus, which is a luminous fungus found in Korea. In this study, we examined the bioluminescence of Omphalotus japonicus mycelia. Light emission was detected at the edges of mycelia grown on solid agar medium. Notably, the intensity of bioluminescence was found to be significantly enhanced following wound induction. The increase in light intensity peaked at 3 h after mechanical damage. We also investigated the effects of extreme temperatures on bioluminescence. Unlike mechanical damage, high and low temperatures repressed the light emission from mycelia. Further investigations are required to reveal the physiological and ecological properties of fungal bioluminescent responses to environmental stresses.

Trehalose가 발광미생물의 동결건조시 생존 및 발광강도에 미치는 영향과 첨가방법에 대한 연구

  • Park, Ji-Eun;Jang, Deok-Jin
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.390-393
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    • 2000
  • In this work, effects of trehalose on the recovery of bioluminescence and viability of luxAB-containing recombinant bacteria were investigated. In case of a recombinant, UV2, only 2.5% of bioluminescence and 2.7% of cell viability were restored after 3.5hr of freeze-drying without trehalose, which implies that cells were heavily damaged during the dehydration. To improve these losses, trehalose was added before freeze-drying on different modes. Trehalose increased the bioluminescence and viability of freeze-dried UV2 in all conditions tested and it was observed that addition of trehalose into the broth(final concentraion, 0.08M) for 15min before the freeze-drying resulted in 45% of bioluminescence and 50% of cell viability. For the other luminescent recombinant, YH9, trehalose showed a similar efficacy.

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The Effect of Storage Temperature on the Viability and Bioluminescence of Photobacterium phosphoreum (저장 온도에 따른 Photobacterium phosphoreum의 Viability와 Bioluminescene)

  • Kim, Hyeon-Suk;Jeong, Seong-Je;Jeong, Gye-Hun;Jeon, Eok-Han
    • KSBB Journal
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    • v.14 no.5
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    • pp.523-527
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    • 1999
  • A naturally luminescent bacterium, Photobacterium phosphoreum was stored in 2.5% NaCl solution at 2$0^{\circ}C$, 4$^{\circ}C$, -2$0^{\circ}C$ and -7$0^{\circ}C$ for 30 days. In vivo luminescence and concentrations of total and culturable cells were determined by luminometer, spectrophotometer and dilution plate counting, respectively. When stored at 4$^{\circ}C$ and 2$0^{\circ}C$, concentrations of cells were rapidly decreased as a result of cell lysis, leading to adrop in turbidity and cultured counts. The bioluminescence of cells stored at 4$^{\circ}C$ was maintained until 12 days while those of cells starved at other temperatures decreased to background level within 3 days. Following incubation of stored cells in fresh liquid medium, activities of viable cells increased throughout storage period excepting cells stored at 2$0^{\circ}C$. Changes in bioluminescence intensity following addition of 2.5% NaCl solution markedly showed in cells stored at -2$0^{\circ}C$ and -7$0^{\circ}C$ and increased to maximum 8 fold.

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Binary Mixture Toxicity of AROCLOR 1248, Oleic Acid, and Elemental Sulfur to Vibrio fischeri Luminescence

  • Kalciene, Virginija;Dabkeviciene, Daiva;Cetkauskaite, Anolda
    • Journal of Environmental Science International
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    • v.24 no.11
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    • pp.1541-1546
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    • 2015
  • The objective of this research was to evaluate the toxicity of the industial xenobiotic Aroclor 1248 (A) and natural origin substances~elemental sulfur (S80) and oleic acid (OA) and their binary mixtures to V. fischeri bioluminescence during the prolonged exposure time (up to 60 min). The bioluminescence quenching test was used to determine the toxic effects. Full factorial experiment design and multiple regression analysis and the comparison of binary mixture effect with the sum of effects of individual chemicals were used for the evaluation of combined effects of toxicants. The analysis of general trend of mixture toxicity to bioluminescence showed that mixture toxic effects were reversible up to 60 min. Data analysis revealed different joint effects, which were depended on mixture composition. S80 enhanced toxic effect of A and acted additively with synergistic interaction. Hydrophobic OA in mixture with A acted antagonistically and in mixture with sulfur caused an additive effect with antagonistic component of interaction. It was concluded that low concentrations of natural toxic substances present in environmental samples as mixtures of chemicals can define the toxicodynamic character of industrial xenobiotics.