Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Application of the Recombinant Bioluminescence Bacterium on the Toxicity Assessment of the Sole Chemicals and Soil Samples

유전자 재조합 생물 발광 균주를 이용한 순수 오염물과 토양시료의 독성도 평가

  • Kong, In-Chul (Department of Environmental Engineering, Yeungnam University) ;
  • Kim, Jin-Yeong (Department of Environmental Engineering, Yeungnam University) ;
  • Lee, Sun-Hee (Department of Environmental Engineering, Yeungnam University) ;
  • Ko, Kyung-Seok (Geologic Environment Division, Korea Institute of Geoscience & Mineral Resources)
  • 공인철 (영남대학교 환경공학과) ;
  • 김진영 (영남대학교 환경공학과) ;
  • 이선희 (영남대학교 환경공학과) ;
  • 고경석 (한국지질자원연구원 지구환경연구본부)
  • Received : 2011.03.31
  • Accepted : 2012.02.24
  • Published : 2012.02.29
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Abstract

Various factors affecting on the bioassay were investigated. Experiments with a low mixture ratio (cell to toxicant solution) of 0.5 : 9.5 (v/v) produced observable bioluminescence intensity for assay. Both sodium lactate and potassium nitrate stimulate bioluminescence activity; 2.6~4.0 times of control. Distilled water and MSM, which gave non significant effects on the bioluminescence activity, were determined as proper diluent or extract solutions. A wide range of toxic responses of metals and organics were observed. In general, organics were much less sensitive than metals. Samples collected from eleven sites showed the bioluminescence activity ranging from 29 to 111% of the control. Significant correlation between toxicity and total metal contents was not observed, but the toxicity of two groups, sorted based on the contaminated arsenic concentration in soils, was 44% and 20%, showing considerable differences.

본 연구에서는 중금속 오염물 생물 검정 수행에 영향을 미치는 조건들에 대해서 조사하였다. 오염 시료의 희석 최소화의 필요 조건인 낮은 균주와 시료의 비율(0.5 : 9.5)에서도 실험을 수행할 정도의 발광 활성이 관찰되었다. Sodium lactate와 $KNO_3$를 첨가한 조건에서 대조군에 대해 약 2.6~4.0배의 발광이 관찰되었다. 시료 희석(추출)용액으로는 발광 영향에 중요한 끼치지 않는 증류수와 MSM이 적절하였다. 본 방법은 중금속에 대해서는 매우 민감하였지만 유기오염물에 대해서는 민감하지 않은 것으로 나타났다. 11지역의 토양 시료추출액은 대조군의 29~111% 발광활성을 나타내었다. 각 시료별 총중금속 농도와 독성 영향 간의 상관성을 예측하기는 어려웠지만 시료의 중요 오염물(비소)에 기준한 두 그룹 간에 통계적 차이의 가능성이 있음을 확인하였다(높은 비소와 낮은 비소 그룹은 각 44%와 20%의 독성도).

Keywords

References

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