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http://dx.doi.org/10.4491/KSEE.2012.34.2.136

Application of the Recombinant Bioluminescence Bacterium on the Toxicity Assessment of the Sole Chemicals and Soil Samples  

Kong, In-Chul (Department of Environmental Engineering, Yeungnam University)
Kim, Jin-Yeong (Department of Environmental Engineering, Yeungnam University)
Lee, Sun-Hee (Department of Environmental Engineering, Yeungnam University)
Ko, Kyung-Seok (Geologic Environment Division, Korea Institute of Geoscience & Mineral Resources)
Publication Information
Journal of Korean Society of Environmental Engineers / v.34, no.2, 2012 , pp. 136-142 More about this Journal
Abstract
Various factors affecting on the bioassay were investigated. Experiments with a low mixture ratio (cell to toxicant solution) of 0.5 : 9.5 (v/v) produced observable bioluminescence intensity for assay. Both sodium lactate and potassium nitrate stimulate bioluminescence activity; 2.6~4.0 times of control. Distilled water and MSM, which gave non significant effects on the bioluminescence activity, were determined as proper diluent or extract solutions. A wide range of toxic responses of metals and organics were observed. In general, organics were much less sensitive than metals. Samples collected from eleven sites showed the bioluminescence activity ranging from 29 to 111% of the control. Significant correlation between toxicity and total metal contents was not observed, but the toxicity of two groups, sorted based on the contaminated arsenic concentration in soils, was 44% and 20%, showing considerable differences.
Keywords
Bioassessment; Bioluminescence; Heavy Metals; Soils; Toxicity;
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