• Molecules and Cells
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• 제42권11호
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• pp.804-809
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• 2019

Oncogenic gain-of-function mutations are clinical biomarkers for most targeted therapies, as well as represent direct targets for drug treatment. Although loss-of-function mutations involving the tumor suppressor gene, STK11 (LKB1) are important in lung cancer progression, STK11 is not the direct target for anticancer agents. We attempted to identify cancer transcriptome signatures associated with STK11 loss-of-function mutations. Several new sensitive and specific gene expression markers (ENO3, TTC39C, LGALS3, and MAML2) were identified using two orthogonal measures, i.e., fold change and odds ratio analyses of transcriptome data from cell lines and tissue samples. Among the markers identified, the ENO3 gene over-expression was found to be the direct consequence of STK11 loss-of-function. Furthermore, the knockdown of ENO3 expression exhibited selective anticancer effect in STK11 mutant cells compared with STK11 wild type (or recovered) cells. These findings suggest that ENO3-based targeted therapy might be promising for patients with lung cancer harboring STK11 mutations.

• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1071-1077
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• 2019

Natural gamma-decalactone (GDL) produced by biotransformation is an essential food additive with a peach-like aroma. However, the difficulty of effectively controlling the concentration of the substrate ricinoleic acid (RA) in water limits the biotransformation productivity, which is a bottleneck for industrialization. In this study, expanded vermiculite (E-V) was utilized as a carrier of RA to increase its distribution in the medium. E-V and three commonly used organic compounds were compared with respect to their effects on the biotransformation process, and the mechanism was revealed. Scanning electron microscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis indicated that RA was physically adsorbed onto the surface of and inside E-V instead of undergoing a chemical reaction, which increased the opportunity for interactions between microorganisms and the substrate. The highest concentration of GDL obtained in the medium with E-V was 6.2 g/l, which was 50% higher than that in the reference sample. In addition, the presence of E-V had no negative effect on the viability of the microorganisms. This study provides a new method for producing natural GDL through biotransformation on an industrial scale.

• Asian-Australasian Journal of Animal Sciences
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• 제4권3호
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• pp.293-298
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• 1991

A study was conducted to determine the effect of environmental temperature and level of food intake on plasma concentration of thyroid hormones. Three dairy heifers were used in an experiment which consisted of three levels of chamber temperature (10, 20 and $30^{\circ}C$) and three levels of food intake (100, 75 and 50% of recommended requirements). The analysis showed significant effects of environmental temperature on plasma triiodothyronine concentration, rectal temperature, respiration rate and heart rate but not on heat production. The range of plasma triiodothyronine was 2.51~1.79 ng/ml when the environmental temperature varied from 10 to $30^{\circ}C$. Effects of feed intake level were significant for heart rate and heal production. Heat production decreased from 25.9 to $20.0kJ/kg^{0.75}{\cdot}h$ when the TDN intake decreased from 66.3 to $35.1g/kg^{0.75}{\cdot}d$. There was no interactive effect of environmental temperature and feed intake level. Plasma triiodothyronine concentration decreased under high environmental temperature without any changes in heat production. The effects of environmental temperature and feed intake level on the physiological function of thyroid gland, as indicated by the relative circulating rate of thyroid hormones, were found to be clear.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.230.2-230.2
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• 2016

A new approach for antimicrobial is based on the overproduction of reactive nitrogen species (RNS), especially; nitric oxide (NO) and peroxinitrite ($ONOO^-$-) are important factors to deactivate the bacteria. Recently, non-thermal atmospheric pressure plasma jet (APPJ) has been frequently used in the field of microbial sterilization through the generation of different kinds of RNS/ROS species. However, in previous study we showed APPJ has combine effects ROS/RNS on bacterial sterilization. It is not still clear whether this bacterial killing effect has been done through ROS or RNS. We need to further investigate separate effect of ROS and RNS on bacterial sterilization. Hence, in this work, we have enhanced NO production, especially; by applying a 1% of HNO3 vapour to the N2 based APPJ. In comparison with nitrogen plasma with inclusion of water vapour plasma, it has been shown that nitrogen plasma with inclusion of 1% of HNO3 vapour has higher efficiency in killing the E. coli and different type of cancer cell through the high production of NO. We also investigate the enhancement of NO species both in atmosphere by emission spectrum and inside the solution by ultraviolet absorption spectroscopy. Moreover, qPCR analysis of oxidative stress mRNA shows higher gene expression. It is noted that 1% of HNO3 vapour plasma generates high amount of NO for killing bacteria and cancer cell killing.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.313-316
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• 2000

When 3 recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B-22-4, $CS13-1.00^{\ast}$ and $CSl3-0.02^{\ast}$, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. Glycine betaine was found to have a strong osmoprotective effect on all 3 rCHO cell lines. Inclusion of 15 mM glycine betaine in hyperosmolar medim enabled rCHO cell lines to grow at 557-573 mOsm/kg where they could not grow in the absence of glycine betaine. However, effect of glycine betaine inclusion in hyperomolar medium on foreign protein production differed among rCHO cell lines. CHO/dhfr-B22-4 cells retained enhanced specific human thrombopoietin (hTPO) productivity in the presence of glycine betaine, and thereby, the maximum hTPO titer obtained at 573 mOsm/kg was increased by 72% over that obtained in the control culture with physiological osmolality (292 mOsm/kg). On the other hand, enhanced specific antibody productivity of $CSl3-1.00^{\ast}$ and $CSl3-0.02^{\ast}$ at elevated osmolality decreased significantly in the presence of glycine betaine at a cost of the recovery of cell growth. As a result, the maximum antibody titer at 557 mOsm/kg was similar to that obtained in the control culture with physiological osmolality. Taken together, efficacy of the simultanous use of hyperosmotic pressure and glycine betaine as a means to improve foreign protein production was variable among different rCHO cell lines.

• 한국가축번식학회지
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• 제19권4호
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• pp.245-250
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• 1996

This study was undertaken to illustrate the uterotonic effect of Leonurus sibiricus. It was dissolved in sterile water and several different dosages were administered both in vitro and in vivo study. Rat uterine tissue for in vitro bioassay was obtained from estrous rat. From the low to high dosages of Leonurus sibiricus were tried and each uterine contraction was recorded and integrated. Anesthetized estrous rat for in vivo study was cannulated into the jugular vein for infusion of the compound. Another cannula with a balloon tipped and water filled was inserted into the uterus to measure uterine activity. While the uterine tissue did not respond to low dosage of compound, high dosage of compound stimulated the tissue to contract less than 1 minute with low amplitude. In vivo rat uterus showed a certain, consistent pattern of contractions which was initial relaxation and followed by prolonged and increased amplitude of contractions. It also caused a short breathing stop which might be due to acute acidosis.

• 한국식품과학회지
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• 제39권5호
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• pp.564-568
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• 2007

본 연구에서는 대장염 유발균인 C, difficile에 대한 항균활성이 우수한 천연항균물질을 검색하기 위하여 40여 가지 생약재 에탄올 추출물에 대하여 C. difficile에 대한 생육저해활성을 측정하였다. 결과, 우슬, 희첨, 황백은 1,000 ppm농도에서 90% 이상의 높은 항균 활성을 나타내었고, 우슬 에틸아세테이트분획물(AJNEA)은 가장 높은 활성을 나타내었으며 이의 최소저해농도(MIC)는 $625{\mu}g/mL$였다. 또한 기능성 소재로의 활용가능성을 확인하기 위하여 AJNEA의 유산균 생육에 미치는 영향을 조사한 결과, B. longum, L. helveticus와 S. thermophilus를 제외한 나머지 균주들에 대해서 생장 억제 효과를 나타내지 않았다. 이들 결과로부터 우슬 추출물은 C. difficile 관련 질환에 대하여 우수한 항균제제로 이용될 수 있을 것으로 확인되었다.

• 아시안잔디학회지
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• 제18권2호
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• pp.71-76
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• 2004

축분 유기질 성분을 고형화된 막대 형태고 조형하여 작물 뿌리 주위에 관입하게 되면 작업과정의 단순화와 비효의 유실을 줄이고 비효 지속 기간을 연장 시키어 작물의 근계 발육에 매우 효과적일 것으로 사료된다. 따라서 본 연구는 화학 비료, 분말 형태의 유기질 비료와 막대형 유기질 비료의 비효를 비교하고 작육의 생장 형태를 분석하여 축분의 막대화에 따른 유기질 비료의 효과지속성을 측정하는데 목적을 두고 실험을 실시하였다. 그 결과, 분말형태의 축분 사용보다는 고형화된 막대형태의 유기질 축분이 비효의 유출을 줄이고 작물 지상부와 근계발달을 촉진시켜 유기질 비료 특유의 완효성 비효의 지속에 효과가 있다는 것이 증명되었다. 특히 멀칭 후에는 분말 형태의 축분 시비가 불가능하나, 이를 막대 형태로 고형화하여 작물 근계 주위에 관입하게 되면 작업과정의 단순화해지며, 수확 후에도 토양 비옥도를 지속적으로 유지 시킬 수 있을 것으로 판단되었다.

• Journal of Ginseng Research
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• 제34권3호
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• pp.205-211
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• 2010

Korean red ginseng (KRG) has been used worldwide as a traditional medicine for the treatment of various diseases, including cancer. In this study, we determined the effect of KRG on the responses of HaCaT cells to peroxynitrite ($ONOO^-$). Cells has been used worldwide as a traditional medicine for the treatment of various diseases, including cancer. In this study, we determined the effect of KRG on the responses of HaCaT cells to peroxynitrite ($ONOO^-$). Cells treated with $ONOO^-$ (2 mM) prior to incubation with control medium for 12 hours displayed reduced viability, as determined using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (viability about 48% of that of non-treated control cells). When KRG was added to the post-incubation medium, the negative effects of $ONOO^-$ on cell viability were significantly reduced. Reverse transcription-polymerase chain reaction analysis indicated that KRG alone did not significantly alter p53 or "growth arrest and DNA damage" (GADD)45 mRNA levels. However, the addition of KRG to the post-incubation medium significantly and dose-dependently reduced levels of p53 and GADD45 mRNA in $ONOO^-$-treated cells. Western blot analyses revealed that incubation with KRG decreased p53 and GADD45 protein levels in $ONOO^-$-treated cells, relative to those in cells incubated with control medium. Collectively, these results suggest that Korean red ginseng extract protects cells against $ONOO^-$-induced genotoxicity by increasing cell viability through modulating the expression of p53 signaling intermediates.

• Korean Chemical Engineering Research
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• 제50권3호
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• pp.556-561
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• 2012

본 연구에서는 고분자형 연료전지(PEMFC) 내의 기체확산층(GDL)에서의 물질 거동 전산해석을 통하여 GDL 물성이 전지성능에 미치는 영향을 알아보았다. GDL 내에서 기상의 산소와 액상의 물의 거동을 계산하기 위하여 multi-phase mixture($M^2$) 모델을 사용하였다. GDL의 접촉각, 기공도, 기체투과도, 두께에 변화를 주며 계산을 실시하여 GDL 내에서의 물질 거동의 변화를 확인 하였고, GDL 물성이 전지성능에 미치는 영향을 파악하였다. 전산해석 결과, GDL의 접촉각과 기공도가 커지고, 두께가 얇아짐에 따라 물질전달 저항이 감소하여 GDL과 촉매층 사이의 계면에서의 물포화도가 낮아지고 산소농도는 증가하여 전지성능이 향상되는 것을 확인하였다.