• Korean Journal of Microbiology
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• v.52 no.2
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• pp.140-147
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• 2016

A process for manufacturing virally-safe porcine bone hydroxyapatite (HA) has been developed to serve as advanced xenograft material for dental applications. Porcine bone pieces were defatted with successive treatments of 30% hydrogen peroxide and 80% ethyl alcohol. The defatted porcine bone pieces were heat-treated in an oxygen atmosphere box furnace at $1,300^{\circ}C$ to remove collagen and organic compounds. The bone pieces were ground with a grinder and then the bone powder was sterilized by gamma irradiation. Morphological characteristics such as SEM (Scanning Electron Microscopy) and TEM (Transmission Electron Microscopy) images of the resulting porcine bone HA (THE Graft$^{(R)}$) were similar to those of a commercial bovine bone HA (Bio-Oss$^{(R)}$). In order to evaluate the efficacy of $1,300^{\circ}C$ heat treatment and gamma irradiation at a dose of 25 kGy for the inactivation of porcine viruses during the manufacture of porcine bone HA, a variety of experimental porcine viruses including transmissible gastroenteritis virus (TGEV), pseudorabies virus (PRV), porcine rotavirus (PRoV), and porcine parvovirus (PPV) were chosen. TGEV, PRV, PRoV, and PPV were completely inactivated to undetectable levels during the $1,300^{\circ}C$ heat treatment. The mean log reduction factors achieved were $${\geq_-}4.65$$ for TGEV, $${\geq_-}5.81$$ for PRV, $${\geq_-}6.28$$ for PRoV, and $${\geq_-}5.21$$ for PPV. Gamma irradiation was also very effective at inactivating the viruses. TGEV, PRV, PRoV, and PPV were completely inactivated to undetectable levels during the gamma irradiation. The mean log reduction factors achieved were $${\geq_-}4.65$$ for TGEV, $${\geq_-}5.87$$ for PRV, $${\geq_-}6.05$$ for PRoV, and $${\geq_-}4.89$$ for PPV. The cumulative log reduction factors achieved using the two different virus inactivation processes were $${\geq_-}9.30$$ for TGEV, $${\geq_-}11.68$$ for PRV, $${\geq_-}12.33$$ for PRoV, and $${\geq_-}10.10$$ for PPV. These results indicate that the manufacturing process for porcine bone HA from porcine-bone material has sufficient virus-reducing capacity to achieve a high margin of virus safety.

• Korean Journal of Plant Resources
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• v.25 no.6
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• pp.682-692
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• 2012

Sea Buckthorn (Hippophae rhamnoides L.) has been used in traditional medicine for the treatment of cough, indigestion, circulatory problems and pain. The associated anti-inflammatory effect of this agent is achieved via the inhibition of Nf-${\kappa}B$ signaling, a property that has been demonstrated to effectively control the symptoms of various skin disorders, including atopic dermatitis. Accordingly, the purpose of this study was to assess the efficacy of Sea Buckthorn in reducing the production of lipopolysaccharide (LPS) activated nitric oxide (NO) by inhibiting the Nf-${\kappa}B$ pathway, as measured by the symptoms of atopic dermatitis (AD) occurring secondarily to inflammation and immune dysregulation. Our data demonstrate that Sea Buckthorn significantly decreased the LPS-induced production of NO (p<0.001). Atopic dermatitis was induced by repeated application of 2,4-dinitrochlorobenzene to the dorsal skin of mice. Topical application of 5% Sea Buckthorn extract improved the symptoms of AD, specifically reducing disease severity scores, scratching behaviors and epidermal thickness. When compared to the control group, animals treated with Sea Buckthorn exhibited increased serum IL-12 levels and decreased serum TNF-${\alpha}$, IL-4 and IL-5 levels. Such a modulation of biphasic T-helper (Th)1/Th2 cytokines may result in a reduction in serum IgE levels. Our findings suggest that mechanism of action of Sea Buckthorn in the treatment of AD is associated with a marked anti-inflammatory effect as well as an inhibition of Th2-mediated IgE overproduction via the modulation of biphasic Th1/Th2 cytokines. Such results suggest that topical Sea Buckthorn extract may prove to be a novel therapy for AD symptoms with few side effects.

• Journal of Bio-Environment Control
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• v.7 no.3
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• pp.191-205
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• 1998

Among cauliflower, ‘Snow king’ and ‘Snow crown’, which are early maturing cultivars, showed active early vegetative growth. However, ‘Snow dress’ showed good curd characteristics and in marketability, ‘Green beauty’was superior and can also be grown in Cheju. All the cauliflower and broccoli Plots transplanted on Sep. 3 and on Sep. 17 had fewer number of days from the planting date to the harvesting date than did the plots transplanted on Oct. 1. h the case of broccoli, those transplanted on Oct. 1 had the highest marketability. In the case of cauliflower cultivars, ‘Snow dress’a late maturing cultivar, demonstrated the greatest reduction in growing stage when cultivated in an unheated plastic film houses and broccoli, growing stage is similar between those grown in an open field and those grown in unheated plastic film houses. For all the cauliflower and broccoli, the contents of vitamin A and C showed no significant difference between those grown in an open field and those grown in unheated plastic film houses.

• Journal of Bio-Environment Control
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• v.29 no.1
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• pp.73-79
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• 2020

The objective of this study is to analyze the heat transfer loss of covering materials in a single-span plastic greenhouse under the steady-state wind environment. To achieve this objective, the following were conducted: (1) design of a small-scaled wind tunnel (SCWT) to analyze heat losses of the greenhouse and its performance; (2) determination of the overall heat transfer coefficient (OHTC) for the covering materials using a small-scaled greenhouse model. The SCWT consists of the blowing, dispersion, steady flow, reduction and testing areas. Each part of the SCWT was customized and designed to maintain air flow at steady state and to minimize the variances in the SCWT test. In this study, the OHTCs of the covering materials were calculated by separating each with the roof, side wall, front and back of the small-scaled greenhouse model. The results of this study show that the OHTC of the roof increases as wind speed increases but the zones in which the increase rate of the OHTC decreased, were distinguished by wind tunnel wing speed of 2 ms^-1. For the side wall, the increase rate of the OHTC was particularly higher in the 0-1 ms^-1 zone.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.91-115
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• 1996

In the recent years, protected horticultural facilities have been modernized and glasshouses are also propagating in Korea, even most vegetables production are conducted in the traditional plastic houses covered with, for example, PVC film for just temperature keeping. It would limit the productivity and competitivity of the vegetable production industry without automatization and high quality year round production. A plant factory, aimed to produce vegetables in the limited areas, was initiated in Christensen farm, Denmark in 1957, and widely propagated in some developed countries. As it has the automatized system which enables to keep optimized environment conditions, it will be the best facility for high quality products as well as year round planned production. However, we have not even started the plant factory production. Since the plant factory is requiring lots of resources, besides plant cultivation technologies, such as environment control, automatic engineering and robotics, our approach to the development of plant factories should be minded on Practical Plant Factories considering our current farming practices and least capital needs rather than blindly employing the advanced technologies from developed countries. Thus, Korean plant factory development can be initiated with year round leaf vegetables production in NFT or DFT cultivation system instead of the moval bed system, in which aerial environment factors such as light, temperature, humidity and CO$_2$ concentration and root environment ones such as solution concentration, temperature, pH and water soluble oxygen shall be automatically controlled. And the seeding, seedling and transplanting operations shall be accomplished in the house entrance, and the harvesting and grading opreations shall be conducted in the house exit. For practical plant factories, environment control technologies including artificial light source, illumination and air conditioning, automatic management for nutrient solution and automatic production line of moval bed system, transplanting and harvest should be developed along with researches on the cost reduction of factory building construction.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.12
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• pp.1-8
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• 2016

This study assessed the feasibility of odor removal by the application of Bacillus sp. that has many advantages in sewage treatment to sewage sludge. The NH3 removal rates in the treatment of primary sludge using only aeration were measured at 24, 48, and 72 hours of treatment and the results were 12.5 %, 12 %, and 42.1 %, respectively. The NH3 removal rates of a reactor injected with BIO-CLOD made by solidifying Bacillus sp. concentrated 10 % together with other substances were measured after 24, 48, and 72 hours of treatment and the results were 43 %, 70 %, and 81 % respectively. In the cases where the Bacillus sp. cultured in NB medium was injected into the primary sludge reactor to reach injection rates of 0 %, 1.7 %, 3.3 %, and 6.7(v/v%), the TVOC removal rates measured when 72 hours had passed after the injection were 59 %, 71 %, 88 %, and 98 % respectively, which were higher than the NH3 removal rates as the NH3 removal rates measured at the same time were shown to be 29 %, 25 %, 31 %, and 48 %, respectively. In the sludge dewaterability conducted with various Bacillus sp. injection concentrations, a Bacillus sp. concentration of 4(v/v%) was considered to be suitable. The Bacillus sp. concentrations and reduction in the bad odor substances were correlated with each other. The results showed that aeration and Bacillus sp. injection will assist biological oxidation so that the bad odor substances can be removed. Based on the SRF values of the primary sludge and digested sludge, in which Alum and PAC were used, the appropriate amount of Alum aggregate reagent was judged to be 500 mg/L, and when PAC was used, 6 mg/L was judged to be appropriate.

• Preventive Nutrition and Food Science
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• v.11 no.1
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• pp.48-53
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• 2006

Although garlic has been reported to have impressive effects in lowering serum lipids, there have been controversial evaluations on these effects. To find the potential fator causing the inconsistency in the previous studies, we examined the effects of two types of garlic according to the producing-area (hangihyung garlic, nangihyung garlic) on serum lipid profiles and leptin level. Thirthy six of 4 wk old Sprague Dawley male rats fed high fat diet (40% of calories as fat) for 6 wks to induce obesity, and subsequently fed 5% garlic powder supplemented (HF+H: hangihyung garlic powder, HF+N: nangihyung garlic powder) high fat diets (w/w) for further 5 wk. For the comparison, normal control group fed AIN-76A diet (11.7% of calories as fat). Supplementation with hangihyung and nangihyung garlic resulted in a significant reduction of high fat induced body weight gain, white fat (i.e., epididymal, visceral and peritoneal fat) development, adipocyte hypertrophy and the development of hyperinsulinemia and hyperliptinemia. Serum triglyceride and total cholesterol level was greatly reduced by hangihyung garlic supplementation (p<0.05). The HDL-cholesterol level was increased by dietary hangihyung and nangihyung garlic. There were slight non-significant decreases in triglyceride and total cholesterol of HF+N group as compared to those of HF group. Leptin level of HF+H group was found to be significantly lower than HF group (p<0.05). There was no significant difference among N group and HF+N group. These results suggest that hangihyung garlic may lead to the higher activity in improving lipid profiles than nangihyung garlic. Whether the hypolipidemic effect of garlic increases in a species-dependent has yet to be determined and awaits further research.

• Journal of Bio-Environment Control
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• v.14 no.1
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• pp.7-14
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• 2005

This study was conducted to develop a chain conveyor-type, row-spacing system for a plant factory. The developed system was evaluated for its practical use in growing plants. Results are intended to provide technical suggestions on the row spacing for hydroponically grown vegetables in a plant factory. The designed row-spacing system for a plant factory could be adjusted by four stages with a conveying speed range of $5.3\~15.8cm{\cdot}s^{-1}$ and with a row-space range of 10-25cm. The torque of driving shaft was measured with a torque range of 11.7-33.3 N$\cdot$m according as a trough weight changes with a range of $17\~935$ N. A measured value was $5.9\~9.8\;N{\cdot}m$ lower than a theoretical value. Travel reduction rate was shown up $1.6\~1.9\%$. The conveying time of trough was shown up 2.24 seconds in case that the designed value was 2.26 seconds. The system was evaluated to be functioning effectively according to the initial design factors in the test.

• Journal of Bio-Environment Control
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• v.5 no.1
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• pp.65-70
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• 1996

Water potential which is an physical chemistry and thermodynamic indicator expressed water status of plant root, stem and fruit could be use as a useful indicator for growth control of hydroponically grown muskmelon plant. Linear relationship was observed between EC and water potential of nutrient solution, consequently increment of EC related to the decreasing water potential and resulted with the alteration of water potential and solute potential of upper leaves. Rapid reduction in growth was observed in over 5${\mu}{\textrm}{m}$ of Cu concentration in the media and same tendency was recorded in the shoot fresh weight, root dry weight and chlorophyll content. Increment of Cu concentration in the nutrient solution leads to lower the growth rate and then the water potential of upper leaves. Turgor pressure was not affected the growth of hydroponically grown muskmelon and also Cu concentration of nutrient solution was not recognized the direct relationship to the growth characteristics of muskmelon. These results demonstrated that water potential of nutrient solution can be use as an useful indicator for water physiological comparison of plant growth in hydroponically grown muskmelon.

• Journal of Bio-Environment Control
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• v.9 no.1
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• pp.20-26
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• 2000

This study aims at analyzing physical and chemical properties of perlite substrate. Particles of substrate were divided into five categories In size: gravel, very-coarse, coarse, medium and fine grade. Particles of very-coarse and greater grades in perlite substrate occupied 98.5％ of total particles. The air phase of total particles was distributed between 76.8％ and 87.7％ with especially showing that of very-coarse grade was lower than that of coarse or smaller one. However, the liquid and solid phases were vice versa. The cation exchange capacity (CEC) measured was highest in the fine grade. In drainage experiments, the water being drained from the substrate increased with the ratio of drain area, and the 65~70％ of total water reduced within five minutes after irrigation. The drained volume was proportional to the depth of the substrate, which contained about 2mL.cm$^2$：by depth (cm). Due to quick reduction of the water in the substrate, the pF value increased in four minutes after irrigation and showed the highest negative correlation ($R^2$＝0.997) with the moisture content of the substrate. The physical and chemical properties including drainage characteristics analyzed in this study can be, therefore, utilized to control the moisture content of perlite substrate, efficiently.